FlyBase:RNAi
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RNAi Design/Analysis Tools
| Resource | Description | Author/Source |
|---|---|---|
| NEXT-RNAi | Software for the design and evaluation of genome-wide RNAi libraries. It performs all steps; from the prediction of specific and efficient RNAi target sites to the visualization of designed reagents in their genomic context. | Boutros lab DKFZ Heidelberg, Germany |
| SnapDragon | A tool for the design of long dsRNAs for specific cell-based gene knockdown in Drosophila melanogaster via an RNAi approach. | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
RNAi Stocks for in vivo studies (fly stocks)
| Resource | Description | Author/Source |
|---|---|---|
| UP-TORR, Updated Targets of RNAi Reagents | A lookup tool for searching BDSC, TRiP, VDRC and NIG-FLY in vivo RNAi collections. | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
| BDSC RNAi Home Page | Links to lists and sublists of RNAi and miRNA sponge insertions at Bloomington. Most, but not all, of the insertions are under the control of UAS and therefore require GAL4 for expression. | Bloomington Drosophila Stock Center (BDSC) Indiana University Bloomington, IN, USA |
| NIG-FLY | NIG-FLY in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request. | NIG-FLY National Institute of Genetics Mishima, Japan |
| TRiP Transgenic RNAi Project | The TRiP Stock Collection contains over 9,000 lines, 1,575 of which correspond to orthologs of human-disease-associated genes. The stocks can be obtained from the BDSC. | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
| THFC, Tsinghua Fly Center | THFC RNAi Stock Collection for triggering RNAi in soma and germline | Tsinghua Fly Center Beijing, China |
| VDRC, Vienna Drosophila Resource Center | The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries | Vienna Drosophila Resource Center (VDRC) Vienna, Austria |
Cell-based RNAi Reagents
| Resource | Description | Author/Source |
|---|---|---|
| DRSC Libraries | Genome-wide libraries for cell-based RNAi screening and validation (on-site or shipped). | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
RNAi Screening Centers
| Resource | Description | Author/Source |
|---|---|---|
| DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) | DRSC/TRiP Functional Genomics Resources at Harvard Medical School provides high throughput RNAi screens. | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
RNAi Results, Validation and Phenotypes
| Resource | Description | Author/Source |
|---|---|---|
| GenomeRNAi | GenomeRNAi is a database containing phenotypes from cell-based and in vivo RNA interference (RNAi) screens in Drosophila. | GenomeRNAi DKFZ Heidelberg, Germany |
| RSVP | RNAi Stock Validation and Phenotypes for in vivo RNAi studies. | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
| Cell-Based RNAi Screen Summary | A summary of public cell-based DRSC screens. | DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA |
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