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Overview
Authors of the papers listed below suggest that they include a technical advance, new type of reagent, or resource likely to be useful for other researchers. Author suggestions have been collected via the Fast-Track Your Paper tool since October 2020, with those most recently submitted at the top. Please note, there will be a lag time before listed papers can be found on FlyBase (FBrf links will become active when available) and the submission date does not necessarily match the publication date of the paper.
Technical Advances
2024
Oct 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| This protocol involves imaging the same larvae class IV neurons without the use of anesthetics and for a short duration of time using spinning disk confocal microscope. | Protocol for Imaging the Same Class IV Neurons at Different Stages of Development. | Bio Protoc. | 39210953 | FBrf0260316 |
| LexAop-PiGM-Iq: optogenetic tool to disrupt G protein alpha subunit q signaling through membrane recruitment of a minimal regulator of G protein signaling (RGS) domain. | Selective optogenetic inhibition of Gαq or Gαi signaling by minimal RGS domains disrupts circuit functionality and circuit formation. | Proc. Natl. Acad. Sci. U.S.A. | 39190348 | FBrf0260277 |
| We present a Hidden Markov Model trained to detect seizure-related behavioral phenotypes in a model for Dup15q syndrome. | Glial expression of Drosophila UBE3A causes spontaneous seizures that can be modulated by 5-HT signaling. | Neurobiol. Disease | 39197537 | FBrf0260426 |
| Synthetic venerose. 1-O-[4-O-(2-aminoethylphosphate)-β-D-galactopyranosyl]-x-glycerol in a R and S isomer mix was custom synthesized by WuXi AppTec Co (China). | A sexually transmitted sugar orchestrates reproductive responses to nutritional stress. | Nat. Commun. | 39353950 | FBrf0260550 |
| Optimisation of hybridisation-chain reaction (HCR) for imaging single molecules of RNA in drosophila larvae. Includes a free-to-use, customisable probe designer tool and protocol details. | Optimization of hybridization chain reaction for imaging single RNA molecules in Drosophila larvae. | Fly | 39351922 | FBrf0260562 |
| New platform for generating histone mutant genotypes for use in epigenetic studies. | Redesigning the Drosophila histone gene cluster: an improved genetic platform for spatiotemporal manipulation of histone function. | Genetics | 39039029 | FBrf0260395 |
| To reach the resolution required to resolve germ granule organization by super resolution microscopy, the distance between the sample and the objective was reduced by slicing the posterior of embryos. | Spatial organization of translation and translational repression in two phases of germ granules. | Nat. Commun. | 39271704 | FBrf0260451 |
| This paper includes a new type of reagents to study protein complex and protein recruitment in cell culture system. | Dachsous and Fat coordinately repress the Dachs-Dlish-Approximated complex to control growth. | J. Cell Biol. | 39373700 | FBrf0260621 |
| Isolation of mitotic and meiotic cell populations by FACS based on the restrictive expression pattern and properties of Bam::GFP and Wcd::RFP transgenic proteins. This allowed processing the RNA for high-throughput sequencing. | Transcriptomic analysis of meiotic genes during the mitosis-to-meiosis transition in Drosophila females. | Genetics | 39225982 | FBrf0260629 |
| This paper shows that BoID technique can be used to identify light-dependent or -induced interaction using Drosophila cell line S2. | Interactions of drosophila cryptochrome. | Photochem. Photobiol. | 38314442 | FBrf0260474 |
| An interactive online application (Meiotic Navigator) compiling new functional data on male fertility and associated testicular microscopy images of 920 evolutionarily conserved spermatocyte genes is available at https://pages.igc.pt/meionav. | The conserved genetic program of male germ cells uncovers ancient regulators of human spermatogenesis. | eLife | 39388236 | FBrf0260647 |
Sep 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Quantitative analysis of both cuticular and gut microbiotas of D. melanogaster after topical challenge with different strains of the entomopathogenic fungus Metarhizium robertsii. | Engineered fungus containing a caterpillar gene kills insects rapidly by disrupting their ecto- and endo-microbiomes. | Commun. Biol. | 39112633 | FBrf0260201 |
| We designed TF-High-Evolutionary (TF-HighEvo), a transcription factor (TF) fused with a base editor (activation-induced deaminase), to continuously induce germline mutations at TF-binding sites across regulatory networks in Drosophila. | TF-High-Evolutionary: In Vivo Mutagenesis of Gene Regulatory Networks for the Study of the Genetics and Evolution of the Drosophila Regulatory Genome. | Mol. Biol. Evol. | 39117360 | FBrf0260285 |
| The method to quantify muscle damage that can be used comparatively across different fly lines and genotypes. | Muscle degeneration in aging Drosophila flies: the role of mechanical stress. | Skelet. Muscle | 39164781 | FBrf0260281 |
| A panel of 12 transgenic gRNA lines targeting different numbers of non-coding genomic sites (individual gRNAs targeting between two and 53 sites in the diploid genome). gRNAs, expressed ubiquitously under the U6 promoter, were inserted into the attP2 site. | Tolerance thresholds underlie responses to DNA damage during germline development. | Genes Dev. | 39054057 | FBrf0260254 |
| A CRISPR/Cas9 method for knock-in and knock-out experiments in S2 cells, whereby an antibiotic-resistance gene is inserted in-frame with the coding region of a gene-of interest. We isolate null cells using antibiotics in feeder layers followed by expansion to make clonal lines. | Generating CRISPR-edited clonal lines of cultured Drosophila S2 cells. | Biol Methods Protoc | 39206452 | FBrf0260333 |
| We have developed a tool (NP-HMMer) to identify neuropeptides based on Hidden Markov Models. Using NP-HMMer we identified phoenixin neuropeptide (CG34242) in Drosophila. | Proteome-wide neuropeptide identification using NeuroPeptide-HMMer (NP-HMMer). | Gen. Comp. Endocrinol. | 39084320 | FBrf0260384 |
| The Double-Headed EP (DEP) transposon, used for insertional mutagenesis, is a P element derivative. The DEP element has Gal4-activatable, outward-directed UAS-promoters at both ends which can be deleted separately in vivo by the FLP or Cre recombinases, respectively. | Dominant suppressor genes of p53-induced apoptosis in Drosophila melanogaster. | G3 (Bethesda) | 38985658 | FBrf0260371 |
| We generated a subtype-specific split-Gal4 for pC1a, a subtype of pC1 neurons. | Male cuticular pheromones stimulate removal of the mating plug and promote re-mating through pC1 neurons in Drosophila females. | eLife | 39255004 | FBrf0260363 |
| Novel concussion model for Drosophila, modified negative geotaxis assay, AI-based tracking of individual flies during negative geotaxis. | Adverse impact of female reproductive signaling on age-dependent neurodegeneration after mild head trauma in Drosophila. | eLife | 39213032 | FBrf0260349 |
Aug 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| New protocol for quantifying vacuoles in fly brain. | Protocol to analyze 3D neurodegenerative vacuoles in Drosophila melanogaster. | STAR Protoc | 38635393 | FBrf0259828 |
| A new PacBio sequencing library construction method named LILAP. | Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes. | Nat. Commun. | 38969648 | FBrf0259990 |
| Technical guidance on dissection and immunolabeling of 3rd instar larvae. | Dissection and Immunolabeling of the Central and Peripheral Nervous System of Drosophila Larvae. | Cold Spring Harb. Protoc. | 37399181 | FBrf0259843 |
| Technical description and protocol for proximity ligation assay in 3rd instar larvae. | Proximity Ligation Assay (PLA) for Fillets of Drosophila Larvae. | Cold Spring Harb. Protoc. | 37399180 | FBrf0259865 |
| Technical description and protocol for cryo-sectioning of Drosophila larvae for greater imaging resolution. | Whole-Larva Cryosectioning and Immunolabeling of Drosophila Larvae. | Cold Spring Harb. Protoc. | 37399178 | FBrf0259906 |
| Con-DAM integrates the Con-Ex (Consumption-Excretion) assay and the DAM (Drosophila Activity Monitor) into a single unit. We expect Con-DAM to serve as an easy method for various purposes that require quantifying food consumption and sleep/activity in the same individual flies. | Con-DAM: Simultaneous measurement of food intake and sleep in Drosophila at the single fly resolution. | MicroPubl Biol | 39005561 | FBrf0259963 |
| DNA barcoding is a reliable method for species identification, while traditional morphological identification struggles with cryptic species. DNA barcoding democratizes scientific research by enabling reproducibility and consistency across global studies. | Species identification of livefood flightless fly (Torinido-shoujoubae) through DNA barcoding. | Ecol. Evol. | 38979002 | FBrf0259947 |
| This protocol provides details on studying plasma membrane stretching in developing Drosophila ovarian cysts using Flipper-TR dye and fluorescence lifetime imaging microscopy. | A protocol for measuring plasma membrane tension in the Drosophila ovary using fluorescence lifetime imaging microscopy. | STAR Protoc | 38489272 | FBrf0259826 |
| 1) Screen for nuclear architecture proteins that are required for peripheral chromatin positioning 2) Interactors of Stwl in cultured cells 3) RNA-seq from female GSC-like cells upon Stwl overexpression 4) CUT&RUN data for genomic targets of Stwl in female GSC-like cells. | A nuclear architecture screen in Drosophila identifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate. | Genes Dev. | 38866555 | FBrf0259861 |
| Software for behavioral phenotyping. | LarvaTagger: manual and automatic tagging of Drosophila larval behaviour. | Bioinformatics | 38970365 | FBrf0260086 |
| Smc1 tag-switch transgene. mCherry tag flanked by FRT sites. Superfolder GFP follows the mCherry cassette. Before FRT expression, Smc1-mCherry is expressed. FRT excision results in expression of Smc1-mCherry. So one can "switch" the tag by inducing FRT expression. | Chromatin-associated cohesin turns over extensively and forms new cohesive linkages in Drosophila oocytes during meiotic prophase. | Curr. Biol. | 38870933 | FBrf0259933 |
| Smc1 tag-switch transgene. mCherry tag flanked by FRT sites. Superfolder GFP follows the mCherry cassette. Before FRT expression, Smc1-mCherry is expressed. FRT excision results in expression of Smc1-mCherry. So one can "switch" the tag by inducing FRT expression. | Chromatin-associated cohesin turns over extensively and forms new cohesive linkages in Drosophila oocytes during meiotic prophase. | Curr. Biol. | 38870933 | FBrf0259933 |
| We developed a proteomic method (VESPA, Variant Enabled SILAC Proteomic Analysis) to test the hypothesis that Drosophila male seminal fluid RNA is translated by females. | Females translate male mRNA transferred during mating. | iScience | 39108707 | FBrf0260210 |
| Transgenic flies with tub-Mer-mNG, tub-MermutCC-mNG, tub-MermutCC-DIX-mNG, tub-MermutCC-FUS-mNG, or tub-GFP-2xOsh2-PH (PI4P reporter). | PI4P-mediated solid-like Merlin condensates orchestrate Hippo pathway regulation. | Science | 39116228 | FBrf0260187 |
| Characterisation of sperm metabolism during sperm storage application of fluorescence lifetime imaging microscopy. | Drosophila melanogaster sperm turn more oxidative in the female. | J. Exp. Biol. | 39023115 | FBrf0260184 |
| cMyc-wt and cMyc-5P were generated in this study. | Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability. | PNAS Nexus | 39161732 | FBrf0260233 |
Jul 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Reporter for recruitment of the Male Specific Lethal dosage compensation complex. | Identification of X chromatin is modulated by complementary pathways in Drosophila melanogaster. | G3 (Bethesda) | 38491905 | FBrf0259697 |
| Gene level promoter evolution rates of genes implicated in transposon silencing, as well as germ cell-specific and somatic cell-specific genes and their relative interspecies expression divergence between D. melanogaster and D. yakuba. | Rapid evolution of promoters from germline-specifically expressed genes including transposon silencing factors. | BMC Genomics | 38977960 | FBrf0259952 |
| Expansion microscopy (ExM) The following transgenic fly lines: dj-(MTS)tdTomato (red-MD) dj-CPV (sperm plasma membrane) UASp-eGFP-hCD63 rubicon-tdTomato (endogenous tag) UASz-rubicon-eGFP UASz-hCD63-tdTomato | Egg multivesicular bodies elicit an LC3-associated phagocytosis-like pathway to degrade paternal mitochondria after fertilization. | Nat. Commun. | 38977659 | FBrf0259998 |
| We generated a number of GFP-tagged alleles of the Cheerio gene including point mutations that affect the mechanical signalling of filamin and small deletion that remove one or more protein domains. | Filamin protects myofibrils from contractile damage through changes in its mechanosensory region. | PLoS Genet. | 38905299 | FBrf0259956 |
| Tripartite FLP recombinase-based conditional transgene expression. | Conditional Inhibition of Eip75B Eliminates the Effects of Mating and Mifepristone on Lifespan in Female Drosophila. | Cells | 38994975 | FBrf0259922 |
| Strains that express dsRNA to additional 1.688[X] repeats. | siRNA that participates in Drosophila dosage compensation is produced by many 1.688X and 359 bp repeats. | Genetics | 38718207 | FBrf0259937 |
| Expressing a human KCNJ2 transgene (either classically-used GFP-tagged version or more recently created HA-tagged version; BDSC #6595 or #92026, respectively) inhibits injury-induced calcium influx in neurons and possibly other cell types. | Calcium plays an essential role in early-stage dendrite injury detection and regeneration. | Prog. Neurobiol. | 38825174 | FBrf0259891 |
| We have designed and manufactured a device to study a highly complex trait such as flight. This device is relatively easy to make and use, as well as inexpensive. We have also developed a suitable software to analyze the traits derived from the videos in a more automated manner. | The relationship between morphology and flight in Drosophila: a study of two pairs of sibling species from a natural population. | Insect Sci. | 37689967 | FBrf0259792 |
| In this paper, we present Au-doped ZnO nanocrystals (NCs) with enhanced biocompatibility. The study reveals luminescent properties of ZnO:Au NCs, suggesting potential for bioimaging and drug delivery tracking. | Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals. | ACS Omega | 38799310 | FBrf0259601 |
Jun 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| This atlas of larval VNC cell types describes immature.(future adult VNC) neurons- organized in a trajectory, and mature interneurons, glia and motoneurons. It links individual clusters to known neurons and uncovers new pathways and genes, i.e. ukar - a new glutamate receptor. | scRNA-seq data from the larval Drosophila ventral cord provides a resource for studying motor systems function and development. | Dev. Cell | 38569548 | FBrf0259499 |
| Novel method for a compound retention in Drosophila, mRNA multiplexing of the Drosophila genes | Sex-specific pharmacokinetic response to phytoestrogens in Drosophila melanogaster. | Biomed. Pharmacotherapy | 38663102 | FBrf0259642 |
| Protocol for Drosophila nervous system imaging. | Ex Vivo Brain Imaging in Drosophila. | Cold Spring Harb. Protoc. | 37197828 | FBrf0259614 |
| Protocol for Drosophila nervous system imaging. | Imaging Olfactory Learning-Induced Plasticity in Vivo in the Drosophila Brain. | Cold Spring Harb. Protoc. | 37197829 | FBrf0259663 |
| Protocol for Drosophila nervous system imaging. | Functional Imaging of Learning-Induced Plasticity in the Central Nervous System with Genetically Encoded Reporters in Drosophila. | Cold Spring Harb. Protoc. | 37197830 | FBrf0259620 |
| Protocol for Drosophila nervous system imaging. | Modeling neurodegenerative and neurodevelopmental disorders in the Drosophila mushroom body. | Learn. Mem. | 38876485 | FBrf0259776 |
| Extracellular superoxide dismutase 3 (SOD3) assay with whole ovaries. | Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster. | Int. J. Mol. Sci. | 38892326 | FBrf0259794 |
May 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We have used the SBF-SEM to visualize the organelle contacts. | ATF4-dependent increase in mitochondrial-endoplasmic reticulum tethering following OPA1 deletion in skeletal muscle. | J. Cell. Physiol. | 38419397 | FBrf0259269 |
| A transgenic chromatin interaction peptide-fused Cas9 exhibits higher mutagenesis efficiency. Additionally, a collection of UAS-sgRNAs transgenic flies have been shown to be valuable for chromoconnectome gene manipulation. | Conditional chemoconnectomics (cCCTomics) as a strategy for efficient and conditional targeting of chemical transmission. | eLife | 38686992 | FBrf0259397 |
| We established improved methods for collecting hemolymph and evaluating its osmolality more accurately and reproducibly. | Refinement of a technique for collecting and evaluating the osmolality of haemolymph from Drosophila larvae. | J. Exp. Biol. | 38634259 | FBrf0259426 |
| We created a suite of new DSCP-based reporter vectors for diverse insect species, leveraging dual compatibility with piggyBac and PhiC31-integration, the 3xP3 universal eye marker, GATEWAY cloning, different colors of reporters and markers, as well as Gal4-UAS binary expression. | A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species. | Sci. Rep. | 38698030 | FBrf0259440 |
| Resource: Drosophila adult eye proteome. | Eye proteome of Drosophila melanogaster. | Proteomics | 37963819 | FBrf0259492 |
| A new drosophila mutant with a loss of function mutation in the gene adipose. The previously used mutant adp[60] line is no longer available and this line adp[1] can replace it in functional studies as it phenocopies the increased fat storage. | A novel adipose loss-of-function mutant in Drosophila. | Fly | 38741287 | FBrf0259509 |
| This paper references the expression patterns of long non-coding RNAs available on the Fly-FISH database (link to database: https://fly-fish.ccbr.utoronto.ca/). | Spatially revealed roles for lncRNAs in Drosophila spermatogenesis, Y chromosome function and evolution. | Nat. Commun. | 38714658 | FBrf0259500 |
| It is a new resource. | Embryonic spatiotemporal expression pattern of Folded gastrulation suggests roles in multiple morphogenetic events and regulation by AbdA. | G3 (Bethesda) | 38366558 | FBrf0259445 |
| A set of IDR alignments across 33 Drosophila genomes where biochemical features of those IDRs have been scored for conservation using models of trait evolution | Evolutionary analyses of intrinsically disordered regions reveal widespread signals of conservation. | PLoS Comput. Biol. | 38662765 | FBrf0259452 |
Apr 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Set of 52 genes were discovered in the paper that interact genetically with rdgB. | A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sites. | Life Sci Alliance | 38499328 | FBrf0259024 |
| Method for building a device for administering time- and depth- calibrated closed-head injury to Drosophila. | dTBI2: A Calibrated, Tunable Device for Administering Traumatic Brain Injury in Drosophila. | Curr Protoc | 38511393 | FBrf0259091 |
| This is a protocol for the mass isolation of staged pupal intestines during Drosophila melanogaster development based on buoyancy in sucrose for biochemical evaluation of protein ubiquitylation. This protocol can be extended to investigate biochemical changes in other tissues. | Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylation. | STAR Protoc | 37950865 | FBrf0258960 |
| Automatic system for recognizing fly courtship. | An automatic system for recognizing fly courtship patterns via an image processing method. | Behav Brain Funct | 38493127 | FBrf0259038 |
| Ex-vivo live imaging of Drosophila pupal midgut intestianl stem cell divisions. | A live-imaging protocol for tracking intestinal stem cell divisions in the Drosophila melanogaster pupal midgut. | STAR Protoc | 38041821 | FBrf0258953 |
| This paper describes an automated robotic microinjection system for Drosophila and zebrafish. | High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot. | Genetics | 38373262 | FBrf0259210 |
| We have developed a tissue-specific O-GlcNAcylation profiling tool to enrich candidate substrates. Through this tool, we mapped the O-GlcNAc interactome and created an O-GlcNAc atlas for various brain regions of Drosophila (tsOGA, http://www.kyuanlab.com/tsOGA). | Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning. | eLife | 38619103 | FBrf0259241 |
| Split inteins are incorporated into Trojan-exon (Diao F et al, 2015) to make gene specific drivers. | Hox gene-specific cellular targeting using split intein Trojan exons. | Proc. Natl. Acad. Sci. U.S.A. | 38602904 | FBrf0259289 |
| A method to synchronise female germline stem cell differentiation in vivo, generating sufficient material for 'omics or biochemistry experiments at specified stages of differentiation. The study includes RNA-seq and Ribo-seq from this material, at 6 stages of differentiation. | Two distinct waves of transcriptome and translatome changes drive Drosophila germline stem cell differentiation. | EMBO J. | 38480936 | FBrf0259258 |
| The use of pseudo-time analysis in single-embryo RNA-sequencing data to determine developmental stage instead of manual embryo staging, thus avoiding human errors. Furthermore, we validated an approach to identify male and female embryos using their transcriptomes. | Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila. | STAR Protoc | 37682716 | FBrf0258985 |
Mar 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Single cell sequencing of pupal to adult midgut development. Provides information about cell types and genes expressed in these as the gut undergoes final adult maturation. | The emergence of circadian timekeeping in the intestine. | Nat. Commun. | 38413599 | FBrf0258833 |
| A transgene encoding a membrane-tethered form of Noktochor, a protein that is normally secreted from astrocytes and neurons to regulate sleep. | Drosophila noktochor regulates night sleep via a local mushroom body circuit. | iScience | 38380256 | FBrf0258837 |
| A collection of cell-type-specific stable-split GAL4 reagents for courtship song neurons. https://splitgal4.janelia.org/precomputed/Lillvis%202023.html | Nested neural circuits generate distinct acoustic signals during Drosophila courtship. | Curr. Biol. | 38295797 | FBrf0258823 |
| A new drug stabilizable GAL80ds variant was created by fusing it to a low-background DHFR-22-DD. | A drug stabilizable GAL80[ds] for conditional control of gene expression via GAL4-UAS and CRISPR-Cas9 systems in Drosophila. | Sci. Rep. | 38467687 | FBrf0259011 |
| Transformer female-specific introns from three speices (D. melanogaster, D. suzukii, and C. capitata) were tested to show female-specificity at different developmental stages in D. melanogaster. This can be used for female-specific expression study. | A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster. | GEN Biotechnol | 38415050 | FBrf0258926 |
| We generated a tool kit to express each Zika virus protein in Drosophila. | A Zika virus protein expression screen in Drosophila to investigate targeted host pathways during development. | Dis. Model Mech. | 38214058 | FBrf0258902 |
| A protocol for studying the neural circuit of female receptivity in Drosophila. | A protocol for measuring the sexual receptivity of female Drosophila. | STAR Protoc | 37703181 | FBrf0259009 |
| We have developed a glyoxal-based fast fixation method for IF, FISH and FISH-IF in Drosophila embryos. We also show that some antibodies like Bic-D, Dynein Heavy Chain and a-Actinin that failed to stain in formaldehyde fixed embryos, were stained beautifully with glyoxal fixation | Glyoxal-based fixation of Drosophila embryos for immunofluorescence staining and RNA in situ hybridization. | STAR Protoc | 37405926 | FBrf0258995 |
| New Ca2+ imaging probes targets to subcellular compartments. | Ca[2+] imaging of synaptic compartments using subcellularly targeted GCaMP8f in Drosophila. | STAR Protoc | 38198278 | FBrf0259069 |
Feb 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Laser capture microdissection of Drosophila Lymph gland. | A conserved nutrient responsive axis mediates autophagic degradation of miRNA-mRNA hybrids in blood cell progenitors. | Nucleic Acids Res. | 37994707 | FBrf0258524 |
| Single-cell transcriptome. | Ribogenesis boosts controlled by HEATR1-MYC interplay promote transition into brain tumour growth. | EMBO Rep. | 38225354 | FBrf0258545 |
| dsRNA complexation with diblock copolymers is a promising strategy for RNAi-based species-specific knock down of gene expression. | Effective delivery and selective insecticidal activity of double-stranded RNA via complexation with diblock copolymer varies with polymer block composition. | Pest Manag. Sci. | 37759365 | FBrf0258536 |
| We present a combined light microscopy, micro-computed tomography (micro-CT), and Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) approach for high-resolution correlative three-dimensional (3D; volume imaging) to characterize internal soft, delicate tissues. | Three-dimensional correlative microscopy of the Drosophila female reproductive tract reveals modes of communication in seminal receptacle sperm storage. | Commun. Biol. | 38321098 | FBrf0258684 |
| We have generated new UAS-APEX2-GBP strains to facilitate high resolution analysis of any GFP-tagged protein that is expressed in somatic D. melanogaster tissues by electron microscopy. | Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling. | Dev. Cell | 38134928 | FBrf0258581 |
| mRNA pellet/EDTA-pellet ratio calculation. Pellet: mRNA-trapping ribosomes; EDTA-pellet: assemblysomes from RNCs. Shift, e.g., elevated DmBLM pellet/EDTA-pellet post-X-ray, indicates assemblysome to ribosome shift, ensuring swift BLM protein access during DNA damage. | 1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster. | Int. J. Mol. Sci. | 38338890 | FBrf0258769 |
| Protocol describes how to perform live imaging of male meiotic cytokinesis in individual spermatocyte cysts and in intact adult testes. | Live Imaging and Analysis of Meiotic Cytokinesis in Drosophila Testes. | Bio Protoc. | 38268980 | FBrf0258608 |
| Comprehensive resource of stocks for genetic analysis of all protein coding genes on the 4th chromosome. | Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks. | Genetics | 37981656 | FBrf0258725 |
| Advanced light sheet microscopy technique based on convolutional neural networks to augment live-imaging data with deep-tissue images taken on fixed samples. | Image restoration of degraded time-lapse microscopy data mediated by near-infrared imaging. | Nat. Methods | 38177507 | FBrf0258736 |
| A method called DISSECT offers the opportunity to extract automatically, in strongly deformed epithelia, a precise characterization of the spatial organization of a given cytoskeletal network combined with morphological quantifications in highly remodeled three-dimensional (3D) epithelial tissues. | DISSECT is a tool to segment and explore cell and tissue mechanics in highly deformed 3D epithelia. | Dev. Cell | 37586367 | FBrf0257870 |
Jan 2024
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Visual Conditional Learning. | A New Behavioral Paradigm for Visual Classical Conditioning in Drosophila. | Bio Protoc. | 37969763 | FBrf0258115 |
| The paper presents an algorithm to quantify image resolution (quality) as a function of depth. The method is demonstrated on microscopy stacks of Drosophila brains using various imaging parameters. An open source ImageJ plugin implementation is made available. | mtFRC: depth-dependent resolution quantification of image features in 3D fluorescence microscopy. | Bioinform Adv | 38146539 | FBrf0258342 |
| This paper describes the methods to express and purify catalytically active Drosophila insulin receptor kinase domain and its mutants from insect cells. Enzyme characterization was done using biochemical techniques including in-vitro kinase and thermal shift assays. | Biochemical characterization of the Drosophila insulin receptor kinase and longevity-associated mutants. | FASEB J. | 38071609 | FBrf0258264 |
| A novel analysis framework for genetic signatures of neuronal phenotypes. | Factorized discriminant analysis for genetic signatures of neuronal phenotypes. | Front. Neuroinform. | 38156117 | FBrf0258365 |
| Expansion microscopy of epithelial tissues (airways) in embryos. | A surfactant lipid layer of endosomal membranes facilitates airway gas filling in Drosophila. | Curr. Biol. | 37992718 | FBrf0258245 |
| TubULAR is a tissue cartography tool that maps whole-organ tissue deformations on dynamic surfaces and decomposes them into interpretable components. | TubULAR: tracking in toto deformations of dynamic tissues via constrained maps. | Nat. Methods | 38057529 | FBrf0258287 |
| This paper develops a graph neural network model for inferring Drosophila gene regulatory networks. Some interactions recorded in flybase are used as evidence to support our computational inferring results. Our model assigns a high prediction score to these gene interactions. | MIGGRI: A multi-instance graph neural network model for inferring gene regulatory networks for Drosophila from spatial expression images. | PLoS Comput. Biol. | 37939200 | FBrf0258170 |
| We generated 3XHA labeled EcI genomic knock-in stock to indicate the expression pattern of EcI. We made UAS-E22O and UAS-E22O-NRT transgenic flies to degenerate the ecdysteroids. We generated phm-T2A-Gal4, spok-Gal80 to specifically manipulate the gene expression in DIOMs. | Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila. | Dev. Cell | 38096823 | FBrf0258487 |
| We construct Pros::GFP KI flies to show the endogenous Pros distribution in pupal intestinal stem cells. | Apical-basal polarity precisely determines intestinal stem cell number by regulating Prospero threshold. | Cell Rep. | 36773292 | FBrf0257653 |
| Generated transgenic UAS-human PPFIA3 reference and variant fly alleles. | A syndromic neurodevelopmental disorder caused by rare variants in PPFIA3. | Am. J. Hum. Genet. | 38181735 | FBrf0258489 |
| We have reported for the first time, the enhancer activity pattern of olf413 in late pupal brain and have demonstrated that olf413 function is essential for motor activity, gravity sensing and spatial balancing of the body against gravity during climbing. | olf413, a putative octopamine biosynthesis pathway gene is required for negative geotactic motor function in Drosophila melanogaster. | J. Genet. | 38073169 | FBrf0258269 |
| We designed a conduction-dominated cryomesh to cryopreserve Drosophila embryos for long-term storage. It increased the hatch rate after rewarming at least 3 times higher than the previous Nylon mesh-based cryopreservation. | Conduction-Dominated Cryomesh for Organism Vitrification. | Adv Sci (Weinh) | 38018294 | FBrf0258552 |
2023
Dec 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| GeneSwitch. | An Adapted GeneSwitch Toolkit for Comparable Cellular and Animal Models: A Proof of Concept in Modeling Charcot-Marie-Tooth Neuropathy. | Int. J. Mol. Sci. | 38003325 | FBrf0258128 |
| New single cell genomics atlas of the Drosophila larval eye disc. | A single cell genomics atlas of the Drosophila larval eye reveals distinct photoreceptor developmental timelines. | Nat. Commun. | 37938573 | FBrf0258045 |
| Miniaturized ultrasound device For Drosophila brain stimulation. | A Miniature Ultrasound Source for Neural Modulation. | IEEE Trans Ultrason Ferroelectr Freq Control | 37812556 | FBrf0257932 |
| New technique: Use of split-GFP to indicate when a transmembrane protein is inserted into the cell membrane. GFP(11) is added to the extracellular domain of the protein of interest; secreted-GFP(1-10) is expressed outside the cell of interest (for example, by fat bodies). | Coordination of Pickpocket ion channel delivery and dendrite growth in Drosophila sensory neurons. | PLoS Genet. | 37943859 | FBrf0258102 |
| Introduced a Yolked Control Paradigm for Drosophila Sleep Deprivation Experiments to Account for the Sleep-Independent Effects of Mechanical Deprivation. | Homeostatic control of deep sleep and molecular correlates of sleep pressure in Drosophila. | eLife | 37906092 | FBrf0258058 |
| CUT&Tag profiling of RNA polymerase II and active and repressive histone modifications in the male germline detailing the timing of gene activation. | Chromosome-specific maturation of the epigenome in the Drosophila male germline. | eLife | 38032818 | FBrf0258215 |
| Materials used in biosensors. | Nanoporous MoS2 Field-Effect Transistor Based Artificial Olfaction: Achieving Enhanced Volatile Organic Compound Detection Inspired by the Drosophila Olfactory System. | ACS Nano | 37902651 | FBrf0258166 |
Nov 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| An alternative method for isolating hemolymph and hemocytes from single Drosophila melanogaster adult flies. | A Simple Protocol for Isolating Hemolymph from Single Drosophila melanogaster Adult Flies. | Methods Protoc | 37888032 | FBrf0257920 |
| Real-time imaging of exosome and dense-core granule biogenesis using Drosophila secondary cells. | A Rab6 to Rab11 transition is required for dense-core granule and exosome biogenesis in Drosophila secondary cells. | PLoS Genet. | 37844085 | FBrf0257910 |
| It describes an open source hardware solution to record and manipulate the behaviour of Drosophila larvae and adults. It describes an open source software to track both on-line and off-line the behaviour of Drosophila larvae and adults. | OptoPi: An open source flexible platform for the analysis of small animal behaviour. | HardwareX | 37795340 | FBrf0257761 |
| A bicistronic transgene in multiple binary expression systems for labeling active zones and cell membranes with only one construct. | SynLight: a bicistronic strategy for simultaneous active zone and cell labeling in the Drosophila nervous system. | G3 (Bethesda) | 37757863 | FBrf0258005 |
| Single-cell RNA sequencing datasets of embryos at gastrulation. | Single-cell transcriptomics illuminates regulatory steps driving anterior-posterior patterning of Drosophila embryonic mesoderm. | Cell Rep. | 37858470 | FBrf0257948 |
| We created an easy to use executable file to analyze data from the DAM system. | HoTDAM! An easy-to-use automated assay expands the inducible thermotolerance phenotype in Drosophila melanogaster: Heat hardening reduces motility. | Comp. Biochem. Physiol. A, Mol. Integr. Physiol. | 37742820 | FBrf0257831 |
Oct 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| A tool called PL-Seq to investigate genes of interest for coordinated 3' alternative polyadenylation and alternative splicing. | Coordination of alternative splicing and alternative polyadenylation revealed by targeted long read sequencing. | Nat. Commun. | 37679364 | FBrf0257510 |
| We describe a protocol to perform RNA FISH on polytene chromosomes, in combination with immunofluorescence, to detect protein-locus colocalization. | A hybrid RNA FISH immunofluorescence protocol on Drosophila polytene chromosomes. | BMC Res. Notes | 37679799 | FBrf0257523 |
| Drosophila as a model organism to determine DNA damage response pathways induced by environmental contaminants. | ATM/Chk2 and ATR/Chk1 Pathways Respond to DNA Damage Induced by Movento[®] 240SC and Envidor[®] 240SC Keto-Enol Insecticides in the Germarium of Drosophila melanogaster. | Toxics | 37755764 | FBrf0257673 |
| C. elegans expressing Drosophila SVIP. | Tubular lysosome induction couples animal starvation to healthy aging. | Nat Aging | 37580394 | FBrf0257584 |
| Panel of Drosophila TAFAZZIN mutants in 10 genetically distinct backgrounds as a new resource for testing emerging therapeutics for the treatment of Barth Syndrome while accounting for the influence of genetic diversity on efficacy. | A novel panel of Drosophila TAFAZZIN mutants in distinct genetic backgrounds as a resource for therapeutic testing. | PLoS ONE | 37756350 | FBrf0257669 |
| Identification of protein interactions | Conformational changes in the negative arm of the circadian clock correlate with dynamic interactomes involved in post-transcriptional regulation. | Cell Rep. | 37043358 | FBrf0257722 |
| Single cell RNA-sequencing dataset of pupal Drosophila hemocytes. | Single-cell transcriptomics identifies new blood cell populations in Drosophila released at the onset of metamorphosis. | Development | 37681301 | FBrf0257675 |
| Behavior tracking protocol. | Methods for Exploring the Circuit Basis of Ethanol-Induced Changes in Drosophila Group Locomotor Activity. | Cold Spring Harb. Protoc. | 37019608 | FBrf0257704 |
| Behavior tracking protocol. | Ethanol Behavioral Responses in Drosophila. | Cold Spring Harb. Protoc. | 37019606 | FBrf0257681 |
| ChIP-nexus, ChIP-seq, ATAC-seq, and MNase-seq data were generated. Mutant ATAC-seq data were generated. Deep learning models trained on ChIP-nexus and ATAC-seq datasets were generated (https://zenodo.org/record/8075860). Binding motifs for transcription factors were mapped. | Chromatin accessibility in the Drosophila embryo is determined by transcription factor pioneering and enhancer activation. | Dev. Cell | 37557175 | FBrf0257743 |
| Live imaging with FUCCI cell cycle phase reporters for limited periods in many cells was used to measure the average duration of each phase and hence, division rate for a given cell population. Also, H2B-RFP pulse-chase. | Spatial regulation of Drosophila ovarian Follicle Stem Cell division rates and cell cycle transitions. | PLoS Genet. | 37747936 | FBrf0257769 |
| Microfluidic technology to automate and standardize the dissociation of CNS tissue into viable neurons for primary culture | Drosophila CASK regulates brain size and neuronal morphogenesis, providing a genetic model of postnatal microcephaly suitable for drug discovery. | Neural Dev. | 37805506 | FBrf0257764 |
| Transcriptional profile of larval and adult astrocytes | Actin-dependent astrocytic infiltration is a key step for axon defasciculation during remodeling. | Cell Rep. | 36790930 | FBrf0257708 |
| Method for making tandem duplications of an existing gene. | Demonstration of in vivo engineered tandem duplications of varying sizes using CRISPR and recombinases in Drosophila melanogaster. | G3 (Bethesda) | 37462278 | FBrf0257718 |
| Detailed instructions on how to work with FRET sensors in intact fly organs. | Metabolic FRET sensors in intact organs: Applying spectral unmixing to acquire reliable signals. | Biol. Open | 37671927 | FBrf0257767 |
| Four supervised machine-learning models were used to analyze the trajectories of three mutants and Canton-S during exploratory activity. | Drosophila genotypes can be predicted from their exploration locomotive trajectories using supervised machine learning. | Behav. Processes | 37717930 | FBrf0257793 |
Sep 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Endogenously tagged Fs(1)h. | Stepwise modifications of transcriptional hubs link pioneer factor activity to a burst of transcription. | Nat. Commun. | 37563108 | FBrf0257271 |
| We describe a novel method for conditional protein aggregation that results in nearly instantaneous protein inactivation. This technique is immediately applicable to any GFP-tagged protein. | Evidence that tissue recoil in the early Drosophila embryo is a passive not active process. | Mol. Biol. Cell | 37405768 | FBrf0257294 |
| Recombination mediated cassette exchange (RMCE) of MiMIC cassette insertion within the Sex Peptide Receptor (SPR) allele for a LexA::QFAD expressing cassette. | Heterogeneous receptor expression underlies non-uniform peptidergic modulation of olfaction in Drosophila. | Nat. Commun. | 37644052 | FBrf0257390 |
| Toxicology Screening approach Applications of metabolomics Applications of genomics. | Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science. | Curr Protoc | 37639638 | FBrf0257380 |
| Optogenetic control over terminal patterning combined with live imaging of target gene transcription and protein accumulation. | Dynamics of an incoherent feedforward loop drive ERK-dependent pattern formation in the early Drosophila embryo. | Development | 37602510 | FBrf0257429 |
| The snRNA-seq dataset has been made publicly available at DRSC/TRiP Functional Genomics Resources data portal (link) to allow users to query the expression of any gene of interest in thoraces. | REPTOR and CREBRF encode key regulators of muscle energy metabolism. | Nat. Commun. | 37582831 | FBrf0257286 |
| We have developed a better system for measurement of thermal preference in Drosophila flies. In our paper we describe the potential pitfalls of a measuring device and demonstrate how to perform thermal preference assay in a careful way. | Wolbachia has subtle effects on thermal preference in highly inbred Drosophila melanogaster which vary with life stage and environmental conditions. | Sci. Rep. | 37612420 | FBrf0257370 |
| Several UAS-driven transgenes, including: - Zdk1 domain targeted to the outer mitochondrial membrane (via dMiro TM domain) for association to LOV2-tagged proteins - mCherry-tagged dMiro (RE isoform) - mCherry/LOV2-tagged dMiro (RE isoform) aa 1-642. | Optogenetic cleavage of the Miro GTPase reveals the direct consequences of real-time loss of function in Drosophila. | PLoS Biol. | 37590319 | FBrf0257379 |
| Python program for designing triplex-forming oligonucleotides for RNA targets. | TFOFinder: Python program for identifying purine-only double-stranded stretches in the predicted secondary structure(s) of RNA targets. | PLoS Comput. Biol. | 37624852 | FBrf0257547 |
| In this study, we developed a system to isolate large amounts of tissues from staged Drosophila melanogaster during development based on buoyancy in sucrose for biochemical evaluation of protein ubiquitination. | PINK1, Keap1, and Rtnl1 regulate selective clearance of endoplasmic reticulum during development. | Cell | 37633267 | FBrf0257587 |
| splitgal4.org (link): This is a centralized database for the gene-specific split-GAL4 lines generated by this study (~100 split-GAL4 lines!) and others in the fly community. | Using single-cell RNA sequencing to generate predictive cell-type-specific split-GAL4 reagents throughout development. | Proc. Natl. Acad. Sci. U.S.A. | 37523539 | FBrf0257150 |
Aug 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Software/code. | Stochastic motion and transcriptional dynamics of pairs of distal DNA loci on a compacted chromosome. | Science | 37384691 | FBrf0256956 |
| We build a substrate-laden wheel upon which Drosophila can walk and lay eggs, while we record neural activity. The wheel may be useful to study other behaviors involving substrate interactions. We also build several chamber setups to study aspects of egg-laying behavior in flies. | A rise-to-threshold process for a relative-value decision. | Nature | 37407812 | FBrf0257087 |
| Gene manipulation in both the Drosophila cardiac model and human induced pluripotent stem cell cardiomyocytes identified interactions between phospholamban/sarcolamban and NCX in the control of cardiac rhythm. | Multiplatform modeling of atrial fibrillation identifies phospholamban as a central regulator of cardiac rhythm. | Dis. Model Mech. | 37293707 | FBrf0257020 |
| Generation of transgenic fly lines that can express human DHX9. | Heterozygous loss-of-function DHX9 variants are associated with neurodevelopmental disorders: Human genetic and experimental evidences. | Eur J Med Genet | 37369308 | FBrf0257121 |
| An automated transport robot that can move crawling larvae around an arena for long-term controlled experiments. | Continuous, long-term crawling behavior characterized by a robotic transport system. | eLife | 37535068 | FBrf0257220 |
| As new resource, in this paper, we report a single-cell transcriptome atlas of Drosophila gastrulae, divided into 77 transcriptomically distinct clusters. With this scRNA-seq data, the genome-wide and accurate spatial patterns of gene expression were also reconstructed. | Single-cell transcriptome atlas of Drosophila gastrula 2.0. | Cell Rep. | 37433294 | FBrf0257125 |
| New approach based on CRIPSPR-Cas9 technique to generate partial or complete deletions of the Y chromosome. | Y chromosome toxicity does not contribute to sex-specific differences in longevity. | Nat. Ecol. Evol. | 37308701 | FBrf0257212 |
| The paper reports stripe as an immediate early gene. It includes data showing that endogenous stripe mRNA or protein expression, or stripe-GAL4 (sr[md710])-driven reporter expression can be used as a neural activity marker. | Cell assembly analysis of neural circuits for innate behavior in Drosophila melanogaster using an immediate early gene stripe/egr-1. | Proc. Natl. Acad. Sci. U.S.A. | 37549285 | FBrf0257229 |
Jul 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Botulinum neurotoxin engineered for Gal4-mediated expression. When expressed in neurons, silences both evoked and spontaneous release. | Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons. | J. Neurosci. | 37221096 | FBrf0256863 |
| We are providing a fully automated computational tool that performs a molecular evolution analysis (detection of positive selection) of any Drosophila melanogaster gene using mostly long-read assemblies of publicly available Drosophila clade genomes. | FREEDA: An automated computational pipeline guides experimental testing of protein innovation. | J. Cell Biol. | 37358475 | FBrf0256908 |
| Software to analyze rhythmic feeding. | CRUMB: a shiny-based app to analyze rhythmic feeding in Drosophila using the FLIC system. | F1000Res. | 37396048 | FBrf0256911 |
| A healthy and stable increased fat storage phenotype in Drosophila melanogaster induced by a protein restricted diet. | A protein restricted diet induces a stable increased fat storage phenotype in flies. | Toxicol Rep | 37396850 | FBrf0256923 |
| Imaging larval tissue differentiation. | Differentiation and function of cardiac valves in the adult Drosophila heart. | J. Exp. Biol. | 37306013 | FBrf0256950 |
| Advanced MARZ algorithm for analysis of DNA sequences. | Investigating the sequence landscape in the Drosophila initiator core promoter element using an enhanced MARZ algorithm. | PeerJ | 37366427 | FBrf0256894 |
| A single-cell atlas of the male first tarsal segment during pupal development. | A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development. | PLoS Biol. | 37379332 | FBrf0256994 |
| Rbp4-Gal4 germline driver that activates in meiotic cells. | Rbp4-Gal4, a germline driver that activates in meiosis, reveals functions for VCP in spermatid development. | Fly | 37436409 | FBrf0257022 |
| FruC fine-tunes gene transcription by promoting low-levels of H3K27me accumulation. | Low-level repressive histone marks fine-tune gene transcription in neural stem cells. | eLife | 37314324 | FBrf0257033 |
| Describes an in vitro morphogen gradient reconstitution system. | Reconstitution of morphogen shuttling circuits. | Sci. Adv. | 37436981 | FBrf0257046 |
| Machine learning-based automatic fly-behavioral detection and annotation (MAFDA) system; anti-FruALL antibody. | Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte Nuclear Factor 4. | Sci. Adv. | 37390217 | FBrf0256900 |
| Optimization of the Gal80DD tool for maximal repression of Gal4 expression of RNAi during developmental stages. | An optimized temporally controlled Gal4 system in Drosophila reveals degeneration caused by adult-onset neuronal Vps13D knockdown. | Front. Neurosci. | 37457002 | FBrf0257041 |
Jun 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| A novel AstA33-Gal4 line that predominantly targets all AstA enteroendocrine cells in the gut but very limited AstA neurons in the brain. | Gut AstA mediates sleep deprivation-induced energy wasting in Drosophila. | Cell Discov. | 37221172 | FBrf0256538 |
| High speed voltage imaging with VsLFM. | Virtual-scanning light-field microscopy for robust snapshot high-resolution volumetric imaging. | Nat. Methods | 37024654 | FBrf0256521 |
| The technique involved forming a whole-cell recording on an MB neuron and applying an electrical stimulus to AL neurons to induce evoked EPSPs from AL to MB. | Efficient strategies based on behavioral and electrophysiological methods for epilepsy-related gene screening in the Drosophila model. | Front. Mol. Neurosci. | 37152436 | FBrf0256425 |
| Proteomic approach to identify changes in cysteine availability using Drosophila cells or tissue samples based on iodo-TMT probes. This technique can be used to identify potential redox changes at specific cysteine residues proteome-wide under different experimental conditions. | Proteome-wide quantitative analysis of redox cysteine availability in the Drosophila melanogaster eye reveals oxidation of phototransduction machinery during blue light exposure and age. | Redox Biol. | 37146512 | FBrf0256685 |
| We developed a machine learning (SVR) model for the Drosophila DPR (downstream promoter region, which spans from +17 to +35 nt relative to the +1 transcription start site). The SVR model can be used to predict the transcriptional activity of any test sequence. | Analysis of the Drosophila and human DPR elements reveals a distinct human variant whose specificity can be enhanced by machine learning. | Genes Dev. | 37163335 | FBrf0256650 |
| Full-length mRNA sequencing in Drosophila tissues (heads, ovaries, embryos at14-16h and 18-22 AEL) and new annotation of transcript isoforms in the indicated tissues. | Sites of transcription initiation drive mRNA isoform selection. | Cell | 37178687 | FBrf0256598 |
| Refined method for charactering the small RNA pools expressed in different cell niche in Drosophila ovaries. The method was originally described in Malone, et al., Cell, 2009 (PMID: 19395010). | The absence of core piRNA biogenesis factors does not impact efficient transposon silencing in Drosophila. | PLoS Biol. | 37279192 | FBrf0256641 |
| Single cell RNAseq resource of adult elav>tauR406W brain tissue. | Tau polarizes an aging transcriptional signature to excitatory neurons and glia. | eLife | 37219079 | FBrf0256723 |
| Live imaging of ex vivo larval NMJ. | Drosophila motor neuron boutons remodel through membrane blebbing coupled with muscle contraction. | Nat. Commun. | 37291089 | FBrf0256774 |
| Genetic tools that allow visualization and genetic manipulations of cells in the lineage of the adult-type fat body (AFB) from early in metamorphosis. | Dynamic de novo adipose tissue development during metamorphosis in Drosophila melanogaster. | Development | 37092314 | FBrf0256560 |
| Resource: Single cell transcriptomic analysis of neuroblast lineages at 105h after larval hatching. | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila. | PLoS Biol. | 37205703 | FBrf0256708 |
| A cell-type specific conditionally tagged DLG1 fly that allows for labeling of excitatory postsynapses in identified cells. | A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in Drosophila. | Cell Rep Methods | 37323572 | FBrf0256821 |
| NOX::GFP reporter line: The Nox::GFP reporter is generated by replacing NoxCRIMIC.TG4.0 allele with the Double Header GFP/T2A-Gal4-coding cassette following the exact cross scheme of Li-Kroeger et al. (2018). | The bHLH-PAS transcriptional complex Sim:Tgo plays active roles in late oogenesis to promote follicle maturation and ovulation. | Development | 37218521 | FBrf0256841 |
May 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We have developed a new software, which is called LabGym, for automated quantification of animal behaviors across species including Drosophila. | LabGym: Quantification of user-defined animal behaviors using learning-based holistic assessment. | Cell Rep Methods | 37056376 | FBrf0256265 |
| Chemical probes for SRS imaging of lacZ and Ggt-1 enzyme activity. | Activatable Raman Probes Utilizing Enzyme-Induced Aggregate Formation for Selective Ex Vivo Imaging. | J. Am. Chem. Soc. | 37057960 | FBrf0256364 |
| A FIJI/ImageJ-based tool to automatically segment and analyze images of active zones and periactive zones of Drosophila neuromuscular junctions. This tool could likely be used for segmentation and analysis of other mesh-like protein distributions within cells. | An approach for quantitative mapping of synaptic periactive zone architecture and organization. | Mol. Biol. Cell | 36542486 | FBrf0256457 |
| scRNA-seq for mitochondrial derivative in Drosophila testis. | Single-cell RNA-sequencing reveals the transcriptional landscape of ND-42 mediated spermatid elongation via mitochondrial derivative maintenance in Drosophila testes. | Redox Biol. | 36933391 | FBrf0256499 |
| TaDa-seq for identification of loci where DAxud1 locates using Dam-DAxud1 fusion protein with Illumina NGS. | Drosophila DAxud1 Has a Repressive Transcription Activity on Hsp70 and Other Heat Shock Genes. | Int. J. Mol. Sci. | 37108646 | FBrf0256390 |
| High resolution chromatin contact Micro-C map on wing imaginal disc. | GAGA-associated factor fosters loop formation in the Drosophila genome. | Mol. Cell | 37003261 | FBrf0256430 |
| A novel optogenetic Gal4-UAS system (ltLightOn) based on a single light-switchable transactivator, termed GAVPOLT, which dimerizes and binds to gene promoters to activate transgene expression upon blue light illumination. | A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila. | ACS Synth. Biol. | 36891673 | FBrf0256042 |
Apr 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Histopathological examination of axonal morphology and Nissl substance using Klüver Luxol Fast Blue-stained tissues. | Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models. | J Evid Based Integr Med | 36866635 | FBrf0255908 |
| This publication describes an operationally simple single-embryo RNA-sequencing methodology, analysis pipeline and the resulting data from the DGRP-737 fly strain. The method lets users assess transcriptional events during fly embryogenesis in a continuous manner. | Continuous transcriptome analysis reveals novel patterns of early gene expression in Drosophila embryos. | Cell Genom | 36950383 | FBrf0256068 |
| New Gal4-driver, active in cardiac valve cells. | Tailup expression in Drosophila larval and adult cardiac valve cells. | genesis | 36546531 | FBrf0256076 |
| (1) large-scale FlyBi ORF clone resource in Gateway format, (2) FlyBi yeast two hybrid data resource, (3) Drosophila Reference Interactome (DroRI), a protein-protein interaction data from multiple datasets at comparable quality accessible at the DRSC MIST online resource. | Next-generation large-scale binary protein interaction network for Drosophila melanogaster. | Nat. Commun. | 37061542 | FBrf0256286 |
| A modified method for time-lapse imaging of ovarian egg chamber explants, adapted to visualize centripetal migration during oogenesis stages 10A-11 | Two phases for centripetal migration of Drosophila melanogaster follicle cells: initial ingression followed by epithelial migration. | Development | 36807509 | FBrf0256237 |
| We prepared a bacteria-conditioned diet in which bacterial products are enriched in a standard yeast-based fly diet. | Recognition of commensal bacterial peptidoglycans defines Drosophila gut homeostasis and lifespan. | PLoS Genet. | 37023169 | FBrf0256280 |
| The article introduces the IMBA (Individual Maggot Behaviour Analyser), a new, free software to track and analyse the behaviour of individual Drosophila larvae tested in groups. This can be used for quick phenotypic analyses of mutations and other genetic manipulations. | High-resolution analysis of individual Drosophila melanogaster larvae uncovers individual variability in locomotion and its neurogenetic modulation. | Open Biol. | 37072034 | FBrf0256288 |
| Cell ablation via laser microsurgery; volume electron microscopy; electrophysiology. | Co-option of epidermal cells enables touch sensing. | Nat. Cell Biol. | 36959505 | FBrf0256245 |
| Streptavidin-based reversible protein retention system, RUSH ("retention using selective hooks"). | Acute manipulation and real-time visualization of membrane trafficking and exocytosis in Drosophila. | Dev. Cell | 37023749 | FBrf0256302 |
| Population recordings of descending neurons during behavior. | Ascending neurons convey behavioral state to integrative sensory and action selection brain regions. | Nat. Neurosci. | 36959417 | FBrf0256179 |
| Generation of an automatically quantified axonal degeneration program based on Python that combines a pre-trained deep-learning masking tool with an axon terminal counting tool. | Direct evaluation of neuroaxonal degeneration with the causative genes of neurodegenerative diseases in Drosophila using the automated axon quantification system, MeDUsA. | Hum. Mol. Genet. | 36611008 | FBrf0256313 |
| Computational approach for transposable element detection in long-reads. | TrEMOLO: accurate transposable element allele frequency estimation using long-read sequencing data combining assembly and mapping-based approaches. | Genome Biol. | 37013657 | FBrf0256182 |
| PGA-dependent OPP incorporation (POPPi) for rapid visualization and identification of nascent protein synthesis. | Rapid cell type-specific nascent proteome labeling in Drosophila. | eLife | 37092974 | FBrf0256333 |
| We present a method and Python module for automatically counting and measuring optical parameters of compound eyes in 2D microscope images and 3D datasets such as those generated by microCT. | Measuring compound eye optics with microscope and microCT images. | Commun. Biol. | 36882636 | FBrf0255994 |
Mar 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| A computational resource for the Drosophila community to consult prior to experimental studies investigating the epigenetic regulatory networks of miRNA-PcG/trxG mediated gene expression. | A genome-wide computational approach to define microRNA-Polycomb/trithorax gene regulatory circuits in Drosophila. | Dev. Biol. | 36596335 | FBrf0255654 |
| Newly developed transgenic lines used in this study help in Live imaging of Muscle stem cells in Drosophila together with neighbouring tissues. | Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster. | Bio Protoc. | 36816994 | FBrf0255866 |
| We have generated novel deletions, duplications, and inversions of the cis-regulatory domains of the bithorax complex using genome editing and frt technology. | CRISPR/Cas9 and FLP-FRT mediated regulatory dissection of the BX-C of Drosophila melanogaster. | Chromosome Res. | 36719476 | FBrf0255637 |
| It showed how apoptosis was affected when the expression level of heme oxygenase was changed. It includes the analyses (assays/experiments) that were exploited to show this particular effect in the fruit fly model organism. | The dual role of heme oxygenase in regulating apoptosis in the nervous system of Drosophila melanogaster. | Front. Physiol. | 36860519 | FBrf0255944 |
| New polyclonal antibody for BRAHMA protein. | CLOCK and TIMELESS regulate rhythmic occupancy of the BRAHMA chromatin-remodeling protein at clock gene promoters. | PLoS Genet. | 36809369 | FBrf0255960 |
| We established a method for accurate and high throughput measurement and sorting of viable Drosophila egg size using large particle flow cytometry. | A high throughput method for egg size measurement in Drosophila. | Sci. Rep. | 36882448 | FBrf0255918 |
| This genetic toolkit can be used to convert Gal4 strains into LexA and Flippase transgenes through simple genetic crosses and fluorescence screening. | A toolkit for converting Gal4 into LexA and Flippase transgenes in Drosophila. | G3 (Bethesda) | 36617215 | FBrf0255992 |
| We generated and described the first protocol to measure the ALR, autophagic lysosomal reformation, in vivo in Drosophila larval tissues. SMER28 has been identified as a positive modulator of ALR in Drosophila and rescues the Drosophila spg15 model. | Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia. | Brain | 36029068 | FBrf0255931 |
| Two new techniques to study glycogen granules: 1) staining in fixed tissues to reveal their subcellular organization 2) a glycogenin-YFP to follow their dynamics live. | Drosophila embryos spatially sort their nutrient stores to facilitate their utilization. | Development | 36805634 | FBrf0256031 |
| 74,000 CNS images with stochastic single neuron (MultiColor FlpOut; MCFO) labeling within GAL4 patterns, searchable by shape at https://neuronbridge.janelia.org and suitable for predicting likely split-GAL4 combinations. | A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution. | eLife | 36820523 | FBrf0256048 |
| Bioinformatic method for identifying functionally active cis-regulatory modules using Cut&Run datasets together with other publicly available chromatin profiling data. | Distinct roles for canonical and variant histone H3 lysine-36 in Polycomb silencing. | Sci. Adv. | 36857457 | FBrf0255964 |
Feb 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We have generated fly lines that express a luciferase reporter construct for clock gene transcriptional activity. The luciferase reporters are FLP-inducible, which enables cell specificity, and luciferase expression is dictated by a minimal E-box sequence from the period gene. | Inducible Reporter Lines for Tissue-specific Monitoring of Drosophila Circadian Clock Transcriptional Activity. | J. Biol. Rhythms | 36495136 | FBrf0255523 |
| New way to immobilize and image the larva. | Bending Drosophila larva using a microfluidic device enables imaging of its brain and nervous system at single neuronal resolution. | Lab Chip | 36537269 | FBrf0255467 |
| We tagged endogenous Runt locus with LlamaTag (Bothma et al., Cell, 2019), which binds to eGFP, to quantify the nuclear concentration of Runt protein in vivo. We reported the concentration dynamics of Runt protein in nuclear cycles of 13 and 14, in males and females. | Predictive modeling reveals that higher-order cooperativity drives transcriptional repression in a synthetic developmental enhancer. | eLife | 36503705 | FBrf0255484 |
| Generation of nanobodies against sarcomeric proteins. | A nanobody toolbox to investigate localisation and dynamics of Drosophila titins and other key sarcomeric proteins. | eLife | 36645120 | FBrf0255573 |
| RNA-seq analysis identified DGC-dependent genes as well as genes whose differential expression patterns under stress are perturbed in DGC mutants. This work is a valuable resource for research into the etiology of muscular dystrophy symptoms. | Signaling through the dystrophin glycoprotein complex affects the stress-dependent transcriptome in Drosophila. | Dis. Model Mech. | 36594281 | FBrf0255683 |
| This paper uses a previously described chemical compound to inhibit the RNA methylase function of the human protein FTSJ1. Here we show that the same compound, called DAP, acts on the orthologous Drosophila proteins Trm7_32 and Trm7_34 (here through larval feeding). | The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance. | Life Sci Alliance | 36720500 | FBrf0255635 |
| Single molecule RNA FISH in third instar Drosophila eye discs. | Buffered EGFR signaling regulated by spitz-to-argos expression ratio is a critical factor for patterning the Drosophila eye. | PLoS Genet. | 36730442 | FBrf0255752 |
| GST-IVTT (PMID: 36102272). | Plk4 Is a Novel Substrate of Protein Phosphatase 5. | Int. J. Mol. Sci. | 36768356 | FBrf0255743 |
| Application of DNA-PAINT super resolution microscopy to study sarcomere morphology. Use of Nanobodies for DNA-PAINT | Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nanoarchitecture in flight muscles. | eLife | 36645127 | FBrf0255622 |
| We present a protocol combining the advantages of high-pressure freezing and freeze substitution and direct stochastic optical reconstruction microscopy to quantify nanotopology of the AZ scaffold protein Bruchpilot at neuromuscular junctions of Drosophila melanogaster. | Single-Molecule Localization Microscopy of Presynaptic Active Zones in Drosophila melanogaster after Rapid Cryofixation. | Int. J. Mol. Sci. | 36768451 | FBrf0255755 |
| Novel method to carry out dye-exclusion assay in adult Drosophila to test for BBB integrity. | Optimization and Technical Considerations for the Dye-Exclusion Protocol Used to Assess Blood-Brain Barrier Integrity in Adult Drosophila melanogaster. | Int. J. Mol. Sci. | 36768206 | FBrf0255731 |
| Single fly quantification of tissue-specific insulin signaling by measuring epitope-tagged phospho-Akt in an ELISA method. | A genetic strategy to measure insulin signaling regulation and physiology in Drosophila. | PLoS Genet. | 36730473 | FBrf0255740 |
| This paper provides a resource of transcriptionally repressive protein domains in Drosophila melanogaster transcription factors. | Systematic identification and characterization of repressive domains in Drosophila transcription factors. | EMBO J. | 36545802 | FBrf0255657 |
| https://bis.zju.edu.cn/DCL/, single-cell RNA landscape of Drosophila. | Construction of a cross-species cell landscape at single-cell level. | Nucleic Acids Res. | 35929025 | FBrf0255582 |
| The catalogue of putative Cp190- and/or CTCF-dependent chromatin insulator elements. | Topological screen identifies hundreds of Cp190- and CTCF-dependent Drosophila chromatin insulator elements. | Sci. Adv. | 36735780 | FBrf0255702 |
| Usage of a single experimental setup that is unique, distinctive and cost-effective using minimal laboratory equipment and resources in order to quantify percent lethality, lifespan and the crawling and climbing behaviour of genetically perturbed or toxicant-exposed Drosophila. | A Reliable and Consistent Method to Quantify Percent Lethality and Life Span in Drosophila melanogaster. | Bio Protoc. | 36789168 | FBrf0255786 |
Jan 2023
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Botulinum neurotoxin engineered for Gal4-mediated expression. When expressed in neurons, silences both evoked and spontaneous release. | A glutamate receptor C-tail recruits CaMKII to suppress retrograde homeostatic signaling. | Nat. Commun. | 36496500 | FBrf0255253 |
| Mass spectrometry analysis of ubiquitination status and chain-type from isolated mitochondria immunofluorescence staining for Serine65 phosphorylated ubiquitin (pS65-Ub) on muscle tissue | Parkin drives pS65-Ub turnover independently of canonical autophagy in Drosophila. | EMBO Rep. | 36250243 | FBrf0255201 |
| Mathematical modelling. | Coupling cell proliferation rates to the duration of recruitment controls final size of the Drosophila wing. | Proc. Biol. Sci. | 36476003 | FBrf0255229 |
| Actin was chased using a new dye. | Synthesis of First Coumarin Fluorescent Dye for Actin Visualization. | Bioconjug. Chem. | 36264777 | FBrf0255043 |
| Removal of single cells from living tissues followed by transcriptome analysis. | Direct isolation of single cells from living brains of Drosophila melanogaster without dissociation for transcriptome analysis. | STAR Protoc | 36181682 | FBrf0255279 |
| Expression of dDAT cDNA in HEK293 suspension cultures using baculovirus for transduction. Measuring substrate and inhibitor affinities on purified protein by Scintillation Proximity Assay (SPA). | Pharmacological Characterization of Purified Full-Length Dopamine Transporter from Drosophila melanogaster. | Cells | 36497070 | FBrf0255233 |
| Protocol for purifications of exosomal proteins using size exclusion chromatography. | Purification of exosome-enriched proteins produced in a Drosophila cell line by size exclusion chromatography. | STAR Protoc | 36386877 | FBrf0255029 |
| The expression of prokaryotic NMN-Deamindase (NMN-D) alters the nicotinamide adenine dinucleotide (NAD+) metabolic flux in vivo, by lowering the precursor nicotinamide mononucleotide (NMN). | The NAD+ precursor NMN activates dSarm to trigger axon degeneration in Drosophila. | eLife | 36476387 | FBrf0255352 |
| ubi-RNase H1::GFP transgenic fly line - C-terminally GFP-tagged coding sequence of RNase H1 (rnh1) expressed from the poly-ubiquitin promoter based pUWG vector. | Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes. | Life Sci Alliance | 36446522 | FBrf0255152 |
| High resolution single cell RNA sequencing resource data from 1-day, 3-day and 7-day old adult eyes from Canton S. Novel cell type specific marker genes were identified for each major eye cell types and some marker genes were validated in vivo. | Single cell RNA sequencing of the adult Drosophila eye reveals distinct clusters and novel marker genes for all major cell types. | Commun. Biol. | 36517671 | FBrf0255275 |
| A new method of recording calcium influx response of tmc-Gal4 labeled md-L neuron in drosophila labellum in vivo, imaging the cell soma in real time and defecting the taste hair to apply mechanical stimuli. | Drosophila proboscis extension response and GCaMP imaging for assaying food appeal based on grittiness. | STAR Protoc | 36386873 | FBrf0255042 |
| A developmental time course of synapse addition in multiple classes of ORNs, PNs, and LNs in the Drosophila antennal lobe represents a new resource for the field. | Synaptic Development in Diverse Olfactory Neuron Classes Uses Distinct Temporal and Activity-Related Programs. | J. Neurosci. | 36446587 | FBrf0255430 |
| Generated transgenic UAS-human EIF4A2 reference and variant fly alleles. | Rare EIF4A2 variants are associated with a neurodevelopmental disorder characterized by intellectual disability, hypotonia, and epilepsy. | Am. J. Hum. Genet. | 36528028 | FBrf0255452 |
| Gene expression evolution tool that is easy to run and should become popular. | Stochastic Modeling of Gene Expression Evolution Uncovers Tissue- and Sex-Specific Properties of Expression Evolution in the Drosophila Genus. | J. Comput. Biol. | 36037023 | FBrf0255437 |
| Hi speed planar illumination microscopy. | Polyphasic circadian neural circuits drive differential activities in multiple downstream rhythmic centers. | Curr. Biol. | 36610393 | FBrf0255503 |
| Ablation of individual cells within a tissue by two-photon microscopy and live imaging of tissue responses with confocal microscopy. | Photoablation at single cell resolution and its application in the Drosophila epidermis and peripheral nervous system. | Front. Physiol. | 36685184 | FBrf0255529 |
| RT&Tag uses antibodies to tether tagmentation reagents in situ in the nucleus for profiling of localized RNAs. The method has been used for profiling RNAs associated with silenced or active chromatin, or with RNA modifications. | The Drosophila embryo as a tabula rasa for the epigenome. | Fac Rev | 36644296 | FBrf0255473 |
2022
Dec 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| A procedure for single molecule RNA fluorescent in situ hybridization for imaging of Clk transcript localization in whole-mount brains (e.g., lLNv, DN), with experiment protocols and an automated Snakemake analysis workflow. Presumed to be adaptable to other transcripts. | Streamlined single-molecule RNA-FISH of core clock mRNAs in clock neurons in whole mount Drosophila brains. | Front. Physiol. | 36439243 | FBrf0255126 |
| Genetically encoded luminescence reporter of distinct circadian clock oscillations in target neurons and tissues, in vivo, in real time. | Real time, in vivo measurement of neuronal and peripheral clocks in Drosophila melanogaster. | eLife | 36190119 | FBrf0254983 |
| 'Expansion Microscopy' is used to improve the resolution of images of cells, and in this study, we developed a technique to determine the mitochondrial morphology within germline stem cells. | Expansion Microscopy-based imaging for visualization of mitochondria in Drosophila ovarian germline stem cells. | FEBS Open Bio | 36331359 | FBrf0255206 |
| The new algorithm Transposable Element Finder (TEF) enables the detection of TE transpositions, even for TEs with an unknown sequence. | Transposable element finder (TEF): finding active transposable elements from next generation sequencing data. | BMC Bioinformatics | 36418944 | FBrf0255150 |
| Auxin inducible-degradation GFP-tagged allele of Med19. | Inducible degradation of the Drosophila Mediator subunit Med19 reveals its role in regulating developmental but not constitutively-expressed genes. | PLoS ONE | 36445897 | FBrf0255104 |
Nov 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Endogenously tagged RNA polymerase II subunits, including mCherry-Rpb1 and EGFP-Rpb3. | Coordinating transcription and replication to mitigate their conflicts in early Drosophila embryos. | Cell Rep. | 36261005 | FBrf0254801 |
| AO-SOLEIL mitigates deep tissue-induced deteriorations and was applied on dSTORM samples in adult Drosophila brains. A 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction was observed. | Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains. | Front. Neurosci. | 36278016 | FBrf0254788 |
| (Z)-4-undecenal is the female pheromone of cosmopolitan D. melanogaster, an oxidation product of the aphrodisiac hydrocarbon (Z,Z)-7,11-heptacosadiene. Females of the Zimbabwe strain release also (Z)-4-nonenal, since they produce another hydrocarbon, (Z,Z)-5,9-heptacosadiene. | The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster. | iScience | 36300000 | FBrf0254861 |
| Population recordings of descending neurons during behavior. | Descending neuron population dynamics during odor-evoked and spontaneous limb-dependent behaviors. | eLife | 36286408 | FBrf0254871 |
| Transgenes encoding HaloTag-based reporters for sparse labeling. | HaloTag-based reporters for sparse labeling and cell tracking. | Fly | 36323649 | FBrf0254955 |
| Expression of different heat shock proteins during defined stages progression of epithelial malignant tumors of different genetic backgrounds. | Delayed discovery of Hsp60 and subsequent characterization of moonlighting functions of multiple Hsp60 genes in Drosophila: a personal historical perspective. | J. Genet. | 36330787 | FBrf0254951 |
| We performed FRET data analysis using Matlab to visualize ATP concentration in living wing disc explants, then described the kinetics of FRET efficiency drop upon use of metabolic drugs targeting glycolysis and/or OxPhos in wt discs or discs with up/down-regulated Hh signaling. | Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc. | EMBO Rep. | 36134875 | FBrf0254957 |
| Protocol for using bioluminescent bacteria to track infection. | Longitudinal monitoring of individual infection progression in Drosophila melanogaster. | iScience | 36345341 | FBrf0254928 |
| In this paper, we made a high-quality and annotated sequencing of Drosophila nebulosa. In addition, we employed CRISPR/Cas9 to disrupt the White gene. This gene was found to be necessary for D. nebulosa mating. | Nebulous without white: annotated long-read genome assembly and CRISPR/Cas9 genome engineering in Drosophila nebulosa. | G3 (Bethesda) | 36063049 | FBrf0254926 |
| New in silico model for cell-cell interaction for Hedgehog signaling. | Predictive model for cytoneme guidance in Hedgehog signaling based on Ihog- Glypicans interaction. | Nat. Commun. | 36163184 | FBrf0254595 |
| RT&Tag uses antibodies to tether tagmentation reagents in situ in the nucleus for profiling of localized RNAs. The method has been used for profiling RNAs associated with silenced or active chromatin, or with RNA modifications. | Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation. | Nat. Methods | 36192462 | FBrf0254922 |
| This paper describes a bioinformatics workflow designed to map insertions of natural transposons in genomes of interest with Genome ARTIST_v2, a software developed by our research team. | Genome ARTIST_v2-An Autonomous Bioinformatics Tool for Annotation of Natural Transposons in Sequenced Genomes. | Int. J. Mol. Sci. | 36293549 | FBrf0254901 |
| We demonstrate stimulated Raman scattering spectro-microscopy driven by a multiple-plate continuum, enable spectroscopic interrogation across the entire Drosophila brain and electronic pre-resonance detection of a fluorescent dye. | Towards stimulated Raman scattering spectro-microscopy across the entire Raman active region using a multiple-plate continuum. | Opt. Express | 36258449 | FBrf0254804 |
| Using 3D electron and light microscopy, we found the mitochondrial network distribution of Drosophila melanogaster strikingly similar to mouse cardiac and oxidative muscles. Proteomic analyses on indirect flight, jump, and leg muscles. | Identification of evolutionarily conserved regulators of muscle mitochondrial network organization. | Nat. Commun. | 36333356 | FBrf0254964 |
| Optogenetic perturbation of aPKC in vivo, based on LARIAT system and GFP-aPKC knock-in line. | aPKC regulates apical constriction to prevent tissue rupture in the Drosophila follicular epithelium. | Curr. Biol. | 36113470 | FBrf0254903 |
| Express Drosophila striated muscle myosin using Sf9/baculovirus system. | Insect Sf9 cells are suitable for functional expression of insect, but not vertebrate, striated muscle myosin. | Biochem. Biophys. Res. Commun. | 36308905 | FBrf0255002 |
| A new stable 8X tissue expansion method and computational tools to reconstruct neural circuit structure in Drosophila using expansion microscopy. All tools are available on GitHub (https://github.com/JaneliaSciComp/exllsm-circuit-reconstruction). | Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy. | eLife | 36286237 | FBrf0255018 |
| New model to analyse significant interactions from Hi-C and micro-C data. | ZipHiC: a novel Bayesian framework to identify enriched interactions and experimental biases in Hi-C data. | Bioinformatics | 35678507 | FBrf0255007 |
| A microfluidic microinjection technique and heartbeat quantification software were introduced which may help to perform cardiac toxicity studies. | An in-vivo microfluidic assay reveals cardiac toxicity of heavy metals and the protective effect of metal responsive transcription factor (MTF-1) in Drosophila model. | 3 Biotech | 36275358 | FBrf0254790 |
Oct 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| The paper describes a new muscle contraction model using Drosophila larvae and optogenetics. | Modeling exercise using optogenetically contractible Drosophila larvae. | BMC Genomics | 36042416 | FBrf0254389 |
| Detailed description of tools for measuring cold tolerance under fictive cold stimuli via programmed optogenetic rearing. | Protocols for measuring cold-evoked neural activity and cold tolerance in Drosophila larvae following fictive cold acclimation. | STAR Protoc | 35776643 | FBrf0254520 |
| LexAop transgenic lines and combining LexA and Gal4 together to study organ communication. | Renal NF-κB activation impairs uric acid homeostasis to promote tumor-associated mortality independent of wasting. | Immunity | 36029766 | FBrf0254493 |
| An improved method of conducting genome-wide mapping of chromatin protein binding sites termed nanoCut&Run. | The nanoCUT&RUN technique visualizes telomeric chromatin in Drosophila. | PLoS Genet. | 36048878 | FBrf0254524 |
| This paper identified variant PRC1 and PRC2.2 complexes in Drosophila using the BioTAP-XL technique. The previously unrecognized conservation of fly and mammalian PcG complexes provides a resource for improved understanding of epigenetic gene regulation in multicellular organisms. | Variant Polycomb complexes in Drosophila consistent with ancient functional diversity. | Sci. Adv. | 36070387 | FBrf0254426 |
| This paper describes a new light-gated expression tool LOV-LexA, in which the transcription factor LexA is shuttled into the nucleus to drive expression of LexAop sequences in the presence of blue light (405nm wavelength) but remains in the cytoplasm in the dark. | Spatial and temporal control of expression with light-gated LOV-LexA. | G3 (Bethesda) | 35876796 | FBrf0254643 |
| New reagent: iRFP-tagged His2Av. | Near-infrared nuclear markers for Drosophila imaging. | MicroPubl Biol | 36212517 | FBrf0254678 |
| Allele dGao[47dsRed] is a platform for RMCE. | Restoration of the GTPase activity and cellular interactions of Gαo mutants by Zn2+ in GNAO1 encephalopathy models. | Sci. Adv. | 36206333 | FBrf0254662 |
| We present tauLUM, a Drosophila model that leverages split- luciferase technology to assay tau multimerization in vivo. TauLUM is a powerful discovery platform for identifying genetic regulators and drugs targeting tau multimerization. | TauLUM, an in vivo Drosophila sensor of tau multimerization, identifies neuroprotective interventions in tauopathy. | Cell Rep Methods | 36160048 | FBrf0254597 |
| This is an open access database for chromosome number and chromosome shape for Drosophila species and other dipterans. | Diptera and Drosophila Karyotype Databases: A Useful Dataset to Guide Evolutionary and Genomic Studies. | Front Ecol Evol | 36238425 | FBrf0254736 |
Sep 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We used shotgun lipidomics and mass-spectrometry to analyze the impacts of vitamin A deficiency on the lipidome and the proteome of the Drosophila eye. | Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome. | Biomolecules | 36008977 | FBrf0254376 |
| Multichannel magnetothermal activation of heat sensors using iron oxide nanoparticles. | Subsecond multichannel magnetic control of select neural circuits in freely moving flies. | Nat. Mater. | 35761060 | FBrf0254201 |
| Botulinum neurotoxin engineered for Gal4-mediated expression. When expressed in neurons, silences both evoked and spontaneous release. | Botulinum neurotoxin accurately separates tonic vs. phasic transmission and reveals heterosynaptic plasticity rules in Drosophila. | eLife | 35993544 | FBrf0254399 |
| High resolution imaging of individual actin filaments in medioapical arrays in amnioserosa cells using grazing incidence, structured illumination. | Superresolution microscopy reveals actomyosin dynamics in medioapical arrays. | Mol. Biol. Cell | 35544300 | FBrf0254444 |
| Hypoxia protocol. | Standardized hypoxia-reoxygenation protocol to assess posthypoxic neurobehavioral impairments and molecular mechanisms in Drosophila melanogaster. | STAR Protoc | 36035795 | FBrf0254331 |
| Tools for effective control over the expression levels of transgenes are largely limited. We report a new series pUASTattB-based plasmids. These plasmids preserve the features of pUASTattB but contain a varying number of UAS sites. | Generation and validation of pX-UASTattB for dose-dependent misexpression studies in Drosophila. | MicroPubl Biol | 36039330 | FBrf0254375 |
| Our study used a novel Rapamycin analog (Rapa* - 3Z) which specifically inhibits dTOR in presence of FKBP12-V55G mutant. | Tissue-restricted inhibition of mTOR using chemical genetics. | Proc. Natl. Acad. Sci. U.S.A. | 36095197 | FBrf0254485 |
Aug 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| A self-supervised deep learning method to analyze fly behaviors. | Selfee, self-supervised features extraction of animal behaviors. | eLife | 35708244 | FBrf0254056 |
| Aion is a bi-stable Anion-conducting Channelrhodopsin allowing sustained silencing of neuronal activity. Aion can be activated with a short blue light pulse (open state half-life ~15min), which can be reversed using orange light. | Aion is a bistable anion-conducting channelrhodopsin that provides temporally extended and reversible neuronal silencing. | Commun. Biol. | 35810216 | FBrf0253932 |
| Positive Cell Cluster Detection method; a devoloped method aims to link the expression of a given transcription factor to the birth-order of an immature motoneuron by using the correlation between the birth-order of motoneuron and their spatial organization. | Post-transcriptional regulation of transcription factor codes in immature neurons drives neuronal diversity. | Cell Rep. | 35767953 | FBrf0253867 |
| Expression of different heat shock proteins during defined stages progression of epithelial malignant tumors of different genetic backgrounds. | Elevation of major constitutive heat shock proteins is heat shock factor independent and essential for establishment and growth of Lgl loss and Yorkie gain-mediated tumors in Drosophila. | Cell Stress Chaperones | 35704239 | FBrf0254080 |
| Spermatogenic single-cyst RNA-seq data, including mitotic, meiotic and post-meiotic stages, of D. melanogaster and D. simulans were presented in this paper, which could be an informative resource to those interested in development, expression evolution, etc. | Spatiotemporal Regulation of a Single Adaptively Evolving Trans-Regulatory Element Contributes to Spermatogenetic Expression Divergence in Drosophila. | Mol. Biol. Evol. | 35687719 | FBrf0254010 |
| deGRadFP construct for targeted degradation of GFP-fusion proteins in freshly laid eggs. | Female meiosis II and pronuclear fusion require the microtubule transport factor Bicaudal D. | Development | 35723263 | FBrf0253939 |
| Consensus docking. | Consensus combining outcomes of multiple ensemble dockings: examples using dDAT crystalized complexes. | MethodsX | 35935527 | FBrf0254184 |
| 1. Generation of a palmitoylome of S2R+ cell line cells 2. Use of BioID to identify interaction partners of DHHC-PAT proteins involved in protein S-palmitoylation. | The S-palmitoylome and DHHC-PAT interactome of Drosophila melanogaster S2R+ cells indicate a high degree of conservation to mammalian palmitoylomes. | PLoS ONE | 35960718 | FBrf0254250 |
| Complexome profiling. | CG7630 is the Drosophila melanogaster homolog of the cytochrome c oxidase subunit COX7B. | EMBO Rep. | 35699132 | FBrf0254233 |
| A bacterial gene encoding for a toxic protein (Kid) that can be used to ablate cells. | A robust and tunable system for targeted cell ablation in developing embryos. | Dev. Cell | 35914525 | FBrf0254323 |
| Antibodies. | Single amino-acid mutation in a Drosophila melanogaster ribosomal protein: An insight in uL11 transcriptional activity. | PLoS ONE | 35981051 | FBrf0254298 |
| 3C method for Drosophila larvae. | Application of the 3C Method to Study the Developmental Genes in Drosophila Larvae. | Front. Genet. | 35910225 | FBrf0254079 |
Jul 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| R-loop mapping in embryos and S2 cells. | R-loop Mapping and Characterization During Drosophila Embryogenesis Reveals Developmental Plasticity in R-loop Signatures. | J. Mol. Biol. | 35609632 | FBrf0253819 |
| Generation of CRISPR/Cas13 plasmids (RxCas13d and PspCas13b) for use in Drosophila. | CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells. | Nucleic Acids Res. | 35244715 | FBrf0253797 |
| Genome edited mutant in the dorsal gene created by CRISPR Cas9 genome editing. | SUMOylation of Dorsal attenuates Toll/NF-κB signaling. | Genetics | 35567478 | FBrf0253870 |
| We developed a method to quantitate the number and cell body position of cell types within organs by combining fluorescent histone fusion reporters, light sheet microscopy and computational tools. | Intact Drosophila central nervous system cellular quantitation reveals sexual dimorphism. | eLife | 35801638 | FBrf0253944 |
| We demonstrated that tissue-specific knockout in parallel with rescue of the knockout by cDNA expression by using the sgRNAs targeting the exon-intron junction of target genes. The sgRNAs/Cas9 cleave the genomic locus of the genes, but not UAS-cDNA transgenes. | CRISPR/Cas9-mediated tissue-specific knockout and cDNA rescue using sgRNAs that target exon-intron junctions in Drosophila melanogaster. | STAR Protoc | 35719725 | FBrf0253824 |
Jun 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| NanoDam is a novel technique enables rapid cell type specific, genome-wide profiling of protein-DNA interactions with high temporal and spatial resolution in a single genetic cross. | NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system. | Dev. Cell | 35483359 | FBrf0253373 |
| We developed a new technique that allow the detection of extracellular ROS ( H2O2) in the Drosophila midgut. | Integrin-ECM interactions and membrane-associated Catalase cooperate to promote resilience of the Drosophila intestinal epithelium. | PLoS Biol. | 35522719 | FBrf0253528 |
| We generated new reagents for the field, please refer to the paper for the reagents generated. | Auxilin regulates intestinal stem cell proliferation through EGFR. | Stem Cell Reports | 35427486 | FBrf0253455 |
| We generated new reagents for the field, please refer to the paper for the reagents generated. | Phosphorylation of Yun is required for stem cell proliferation and tumorigenesis. | Cell Prolif | 35437864 | FBrf0253603 |
| This protocol paper describes how to differentially purify cytoplasmic and nuclear ribonucleoprotein (RNP) complexes from adult Drosophila heads. | An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclear Drosophila head fractions. | STAR Protoc | 35634357 | FBrf0253600 |
| Here, we described and characterized a low-cost, easy-to-construct device to enable students to record rapid Drosophila melanogaster (fruit fly) behaviors during optogenetics experiments. | An optogenetics device with smartphone video capture to introduce neurotechnology and systems neuroscience to high school students. | PLoS ONE | 35522662 | FBrf0253392 |
| Isolation and quantitatvie analysis of fly cuticular microbiotas. | Microbiome assembly on Drosophila body surfaces benefits the flies to combat fungal infections. | iScience | 35663020 | FBrf0253684 |
| All-optical approach to study olfactory system and glomeruli: optogenetic activation of glomeruli and calcium imaging readout. | All-optical manipulation of the Drosophila olfactory system. | Sci. Rep. | 35595846 | FBrf0253539 |
| Nuclear Matrix (NuMat) is a biochemically defined entity that provides a snap shot of the features of nuclear architecture. Here we present a protocol to isolate and visualize NuMat in situ in intact embryos or tissues of Drosophila melanogaster and its applications. | In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture. | STAR Protoc | 35600936 | FBrf0253551 |
| Hi speed planar illumination microscopy. | Regulation of PDF receptor signaling controlling daily locomotor rhythms in Drosophila. | PLoS Genet. | 35605015 | FBrf0253689 |
| CRISPR-Cas9 genome editing was used to create a new sog knock out mutant in which the sog 5' untranslated region was partially removed, along with sequence encoding the sog start codon and signal peptide, which were replaced with an attP recombination sequence (sogattP). | Modelling the structure of Short Gastrulation and generation of a toolkit for studying its function in Drosophila. | Biol. Open | 35603711 | FBrf0253731 |
| In this study we describe SpyChIP, which depends on covalent isopeptide bond between two peptides SpyTag and SpyCatcher, to identify sites of cell type-specific transcription factor (TF) occupancy in native physiological contexts without tissue dissociation or nuclei sorting. | SpyChIP identifies cell type-specific transcription factor occupancy from complex tissues. | Proc. Natl. Acad. Sci. U.S.A. | 35696584 | FBrf0253726 |
| Live imaging of ex-vivo testis with DNA and lysosome markers to explore phagoptosis. | The phagocytic cyst cells in Drosophila testis eliminate germ cell progenitors via phagoptosis. | Sci. Adv. | 35714186 | FBrf0253775 |
| We developed a system modeling early neurodegenerative events in Drosophila adult photoreceptor cells. Thanks to the stereotypy of their axonal projections, this system delivers quantitative data on sporadic and progressive axonal degeneration of photoreceptor cells. | A Quantitative Model of Sporadic Axonal Degeneration in the Drosophila Visual System. | J. Neurosci. | 35534228 | FBrf0253774 |
May 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| New fluorescent phenotypic markers. Balancer chromosomes for Drosophila simulans. | Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species. | G3 (Bethesda) | 35143616 | FBrf0253138 |
| The paper offers a computational model that recreates the posterior localization of nanos and polar granule component mRNAs in the oocyte and early embryo. | Computational modeling offers new insight into Drosophila germ granule development. | Biophys. J. | 35288123 | FBrf0253258 |
| Measurement of mitochondrial respiratory chain enzymatic activities in D. melanogaster samples. | Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples. | STAR Protoc | 35479112 | FBrf0253336 |
| iPOND identification of replication fork associated proteins in 3-5h embryos and S2 cell lines by quantitative mass spectrometry. | Identification of replication fork-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry. | Sci. Rep. | 35484306 | FBrf0253333 |
| Our paper describes HyDrop, a completely open-source droplet-microfluidic single-cell sequencing method. HyDrop can work in scRNA-seq or scATAC-seq mode, and has been tested on human, mouse and drosophila samples. | Hydrop enables droplet-based single-cell ATAC-seq and single-cell RNA-seq using dissolvable hydrogel beads. | eLife | 35195064 | FBrf0253117 |
| We describe optimized assay for transposase-accessible chromatin by sequencing (ATAC-seq) library preparation using nuclei isolated from adult Drosophila brains. This optimized protocol will facilitate future ATAC-seq studies using adult flies. | Optimized assay for transposase-accessible chromatin by sequencing (ATAC-seq) library preparation from adult Drosophila melanogaster neurons. | Sci. Rep. | 35411004 | FBrf0253212 |
| Two new mutant alleles, Yippee[Chi-A] (BL#93858) and Yippee[Delta1] (BL#93859). | A fly appeared: sable, a classic Drosophila mutation, maps to Yippee, a gene affecting body color, wings, and bristles. | G3 (Bethesda) | 35266526 | FBrf0253414 |
| Development of a CRISPR/Cas9-based knock-in method to target genes in the Drosophila genome. We also present an updated antennal lobe atlas based on our findings. | Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. | eLife | 35442190 | FBrf0253272 |
| New plasmid and Drosophila lines to overexpress transgenes in Drosophila. | A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila overexpression transgenes. | Sci. Rep. | 35264662 | FBrf0252918 |
| Single-cell wounding model, epidermis. | Calcium bursts allow rapid reorganization of EFhD2/Swip-1 cross-linked actin networks in epithelial wound closure. | Nat. Commun. | 35524157 | FBrf0253404 |
| Automated, high-resolution quantitative phenotyping of Drosophila intestines based on cell segmentation data. Using cell coordinates, the intestines are computationally linearized and aligned to enable comparative analysis along their whole length. | An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut. | Cell Rep Methods | 35474669 | FBrf0253351 |
| The effect of Patronin-RNAi on the microtubule polarity of Class 4 neurons can be assayed using the recombinant line: ppk-gal4, UAS-EB1-TagRFP-T, UAS-Patronin-RNAi (BL36659)/TM6. | Microtubule polarity is instructive for many aspects of neuronal polarity. | Dev. Biol. | 35341730 | FBrf0253310 |
| UAS-NCDN (Neurochondrin) transgenic flies. | Regulation of the evolutionarily conserved muscle myofibrillar matrix by cell type dependent and independent mechanisms. | Nat. Commun. | 35562354 | FBrf0253445 |
| A new anti-Frl antibody was generated in rat after immunization with a purified recombinant protein containing the amino acid residues 687-1183 of Frl. | A Comparative Study of the Role of Formins in Drosophila Embryonic Dorsal Closure. | Cells | 35563844 | FBrf0253495 |
Apr 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| This paper utilizes FIJI macros to quantify cachectic phenotypes such as muscle detachment and lipid droplet size. | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae. | STAR Protoc | 35284841 | FBrf0252884 |
| SoAL: tool for social behavior analysis. SDPD-15k: fly social behavior training dataset for SoAL. MIAS: software for video acquisition. | Behavioral signatures of structured feature detection during courtship in Drosophila. | Curr. Biol. | 35139360 | FBrf0253010 |
| This paper reports a CRISPR/Cas9 edited genomic mutant of Jra (FBgn0001291) resistant to SUMO conjugation on Lysines 29 and 190. The resultant fly line shows a phenotype consistent with increased activity of Jra. | SUMOylation of Jun fine-tunes the Drosophila gut immune response. | PLoS Pathog. | 35255103 | FBrf0252954 |
| We generated new reagents for the field, please refer to the paper for the reagents generated. | Derlin-1 and TER94/VCP/p97 are required for intestinal homeostasis. | J. Genet. Genomics | 34547438 | FBrf0253107 |
| 6 strains were created or customized for enabling recombination on the 4th chromosome. | New resources for the Drosophila 4th chromosome: FRT101F enabled mitotic clones and Bloom syndrome helicase enabled meiotic recombination. | G3 (Bethesda) | 35084488 | FBrf0253111 |
| New resource: measured synaptic surface areas in a variety of neurons in the Drosophila first instar larval central nervous system. | Synaptic counts approximate synaptic contact area in Drosophila. | PLoS ONE | 35377898 | FBrf0253082 |
| Tracking of larval locomotion for long periods. | Long-Term Observation of Locomotion of Drosophila Larvae Facilitates Feasibility of Food-Choice Assays. | Adv Biol (Weinh) | 34365739 | FBrf0253215 |
| S2R+ cells with stable "LiveDrop" reporters (GFP and mRFP) that marks the surface of lipid droplets. | Recruitment of Peroxin 14 to lipid droplets affects lipid storage in Drosophila. | J. Cell Sci. | 35274690 | FBrf0253163 |
| Automated pipeline for quantifying interactions between adjacent glial subsets. | Quantifying Glial-Glial Tiling Using Automated Image Analysis in Drosophila. | Front. Cell. Neurosci. | 35401121 | FBrf0253225 |
| Behavioral method for measuring solid food consumption. | Identification of additional dye tracers for measuring solid food intake and food preference via consumption-excretion in Drosophila. | Sci. Rep. | 35418664 | FBrf0253152 |
Mar 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Modified TUNEL protocol to study cell death in tissues. | Protocol to study cell death using TUNEL assay in Drosophila imaginal discs. | STAR Protoc | 35128481 | FBrf0252563 |
| We developed a PCR-based NGS protocol to cluster cell lines based on the genome-wide distribution of a limited number of diagnostic TE families. It can accurately identify and authenticate the numerous Drosophila cell lines. | A novel transposable element-based authentication protocol for Drosophila cell lines. | G3 (Bethesda) | 34849844 | FBrf0252561 |
| It is improvement on culturing wing imaginal disc and live imaging cytonemes, specially the ones from the Air Sac Primordium. It also contains a follow up Cytoneme Dynamics analysis tool for ImageJ/FIJI and a R/RStudios pipeline for the data analysis and graphical representation. | Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes. | STAR Protoc | 35141564 | FBrf0252689 |
| Use of DLM spiking readouts. | Distinct Aging-Vulnerable and -Resilient Trajectories of Specific Motor Circuit Functions in Oxidation- and Temperature-Stressed Drosophila. | eNeuro | 34876473 | FBrf0252428 |
| We developed a CRISPR/Cas9-based knockin system (LexA.G4HACK) to replace Gal4 coding sequences with LexA, and a LexAop-based short hairpin RNA expression vector (pWALEXA20) to achieve short hairpin RNA-mediated gene silencing. | Transgenic Drosophila lines for LexA-dependent gene and growth regulation. | G3 (Bethesda) | 35100369 | FBrf0252870 |
| A new high-speed imaging method to record ultra-fast photomechanical photoreceptor microsaccades in living flies. | High-speed imaging of light-induced photoreceptor microsaccades in compound eyes. | Commun. Biol. | 35241794 | FBrf0252822 |
| NMR-based profiling of polar and non-polar metabolites in dissected larval tissues or whole animals. | A toolbox to study metabolic status of Drosophila melanogaster larvae. | STAR Protoc | 35252884 | FBrf0252824 |
| Deep single-cell ATAC-seq dataset of mesoderm / muscle cell fate during embryonic development. | Simultaneous cellular and molecular phenotyping of embryonic mutants using single-cell regulatory trajectories. | Dev. Cell | 35176234 | FBrf0252873 |
| We used a new CRISPR Drosophila tafazzin mutant line. This line has the first 889 basepairs removed from the first exon of the tafazzin gene. Additionally, this line has a RFP marker knocked in. | NAD supplementation improves mitochondrial performance of cardiolipin mutants. | Biochim Biophys Acta Mol Cell Biol Lipids | 35051613 | FBrf0252704 |
| Real-time optical recording of the formation of a visual memory trace in the ellipsoid body. | Differential mechanisms underlie trace and delay conditioning in Drosophila. | Nature | 35173333 | FBrf0252922 |
| Reagents for new spliceform generated. | Septins tune lipid kinase activity and PI(4,5)P2 turnover during G-protein-coupled PLC signalling in vivo. | Life Sci Alliance | 35277468 | FBrf0252936 |
| The following are new resources generated in this paper. 1. Rat monoclonal anti-FruM antibody (clone 1C1) 2. Rat monoclonal anti-FruM antibody (clone 1A8) 3. Rat monoclonal anti-FruALL antibody (clone 2E4) 4. Rat monoclonal anti-FruALL antibody (clone 3G11D12). | Mutually exclusive expression of sex-specific and non-sex-specific fruitless gene products in the Drosophila central nervous system. | Gene Expr. Patterns | 35124238 | FBrf0252913 |
| High resolution microscopy and ultra-sensitive proteomics of nephrocytes. | Scaffold polarity proteins Par3A and Par3B share redundant functions while Par3B acts independent of atypical protein kinase C/Par6 in podocytes to maintain the kidney filtration barrier. | Kidney Int. | 34929254 | FBrf0252977 |
Feb 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Computational tools for the analysis of loosely-coupled patterns of neuronal activity and for detecting phases in the execution of rhythmic behaviors. | Extracting temporal relationships between weakly coupled peptidergic and motoneuronal signaling: Application to Drosophila ecdysis behavior. | PLoS Comput. Biol. | 34910730 | FBrf0252232 |
| Stable Isotope Labelling of Amino Acids in Flies (SILAF) is a cost-effective and highly efficient SILAC technique, adapted to Drosophila laboratory needs. It is based on a holidic food source and achieves 99.5% labelling efficiency with heavy lysine at 15$ per mg of protein. | Stable Isotope Labeling of Amino Acids in Flies (SILAF) Reveals Differential Phosphorylation of Mitochondrial Proteins Upon Loss of OXPHOS Subunits. | Mol. Cell. Proteomics | 33640490 | FBrf0252195 |
| New Drosophila transgenic lines generated that can be available at request. | Selective autophagy controls innate immune response through a TAK1/TAB2/SH3PX1 axis. | Cell Rep. | 35081354 | FBrf0252505 |
| We have developed allelic drive based transgenic fly that can target the insecticide resistant para 1014F locus. We have also developed a fly line containing 3xP3GFP insertion at para L1014 locus. | Reversing insecticide resistance with allelic-drive in Drosophila melanogaster. | Nat. Commun. | 35022402 | FBrf0252383 |
| This paper reports a new direct-drive btl-GFP reporter that can be used to follow tracheal morphology independently of the GAL4/UAS system. | A direct-drive GFP reporter for studies of tracheal development in Drosophila. | Fly | 35094652 | FBrf0252525 |
| This manuscript describes the use of micro-computerized tomography (microCT) for measuring the size and morphology of the Drosophila heart. This technique is especially useful for analyzing cardiac hypertrophy in flies. | Analysis of Drosophila cardiac hypertrophy by microcomputerized tomography for genetic dissection of heart growth mechanisms. | Am J Physiol Heart Circ Physiol | 34951542 | FBrf0252376 |
| Additions to FlyAtlas 2, Drosophila melanogaster gene expression resource: 1. Profile Search facility, 2. Category Search facility, 3. Additional Tissues, 4. Specialized section on larval midgut pH regions. | FlyAtlas 2 in 2022: enhancements to the Drosophila melanogaster expression atlas. | Nucleic Acids Res. | 34718735 | FBrf0252311 |
| A new resource: The mRNA expression (bulk RNA sequencing) of third instar larval ovaries (wild type-Oregon R) through three developmental time points. In addition the mRNA expression of somatic and germ line tissue (separated through Flow sorting) of the ovary in these stages. | Distinct gene expression dynamics in germ line and somatic tissue during ovariole morphogenesis in Drosophila melanogaster. | G3 (Bethesda) | 34849771 | FBrf0252584 |
| Python-based code used to quantify object-to-object distances; e.g., mRNA to centrosomes. | Drosophila centrocortin is dispensable for centriole duplication but contributes to centrosome separation. | G3 (Bethesda) | 35100335 | FBrf0252569 |
| A versatile toolkit focused on piRNA biology and transposon biology that combines germline transgenic RNAi, GFP marker lines for key proteins of the piRNA pathway. | A genetic toolkit for studying transposon control in the Drosophila melanogaster ovary. | Genetics | 34718559 | FBrf0252592 |
| A new method for accessing ovarian maturation by quantifying the amount of yolk with YP1::GFP reporter. | Effects of Food and Temperature on Drosophila melanogaster Reproductive Dormancy as Revealed by Quantification of a GFP-Tagged Yolk Protein in the Ovary. | Front. Physiol. | 35046840 | FBrf0252438 |
| Characterization of the role of Naa40 in lipid metabolism in Drosophila, specifically in the larval fat body. | Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis. | BMC Biol. | 35057804 | FBrf0252501 |
| Using NanoLuc Binary Technology, we first developed an assay to detect ligand-dependent heterodimerization of the ecdysone receptor (EcR) and retinoid X receptor (RXR) in HEK 293T cells. We also developed HEK293 cells that stably express Ecdysone Importer. | Rapid Assessment of Insect Steroid Hormone Entry Into Cultured Cells. | Front. Physiol. | 35145429 | FBrf0252694 |
Jan 2022
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Development of an automated microinjection system for Drosophila embryos. | Multiscale, multi-perspective imaging assisted robotic microinjection of 3D biological structures. | Annu Int Conf IEEE Eng Med Biol Soc | 34892294 | FBrf0252055 |
| We provide two macros to quantify exosome release and autophagy in the Drosophila Neuromuscular junction. | Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster. | Front. Cell Dev. Biol. | 34869373 | FBrf0252025 |
| The transgenic flies overexpress dEGFR specifically within somatic intestinal stem cells (ISC) and enteroblasts (using the EsgGal4, GFP driver). | Membrane therapy using DHA suppresses epidermal growth factor receptor signaling by disrupting nanocluster formation. | J. Lipid Res. | 33515553 | FBrf0252238 |
| Genome sequences for D. suzukii individuals and population genomics analysis. | Population genomics of Drosophila suzukii reveal longitudinal population structure and signals of migrations in and out of the continental United States. | G3 (Bethesda) | 34599814 | FBrf0252078 |
| Cell line authentication using transposable element insertions. | Transposable element profiles reveal cell line identity and loss of heterozygosity in Drosophila cell culture. | Genetics | 34849875 | FBrf0252038 |
| Utilizing a "lab-in-a-box" mechanism to increase accessibility of research opportunities for undergraduates. | Flying in the Face of Adversity: a Drosophila-Based Virtual CURE (Course-Based Undergraduate Research Experience) Provides a Semester-Long Authentic Research Opportunity to the Flipped Classroom. | J. Microbiol. Biol. Educ. | 34880963 | FBrf0252050 |
| Single molecule localization microscopy to visualize SNAP and HALO-tagged endogenous protein in tissue samples. Using the LifeAct peptide for super-resolution imaging of actin organization. | A single-molecule localization microscopy method for tissues reveals nonrandom nuclear pore distribution in Drosophila. | J. Cell Sci. | 34806753 | FBrf0252184 |
| GRO-Seq of Stage 5 Drosophila melanogaster embryos is included in the paper. We believe this would be a useful resource for other researchers. | Histone variant H2A.Z regulates zygotic genome activation. | Nat. Commun. | 34853314 | FBrf0252039 |
| Using mt-keima, we tried to analyze the incidence of mitophagy in Keyon cells. | Mitochondrial aconitase 1 regulates age-related memory impairment via autophagy/mitophagy-mediated neural plasticity in middle-aged flies. | Aging Cell | 34799973 | FBrf0252217 |
| UAS-TM4SF. | Fos regulates macrophage infiltration against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila. | PLoS Biol. | 34990456 | FBrf0252327 |
| We utilized the Dorsal-Eye Gal4 (DE-GAL4) to drive the exosome marker GFP-CD63 in the dorsal half of the eye imaginal discs. We detect the GFP-CD63 puncta in the morphogenetic furrow on the ventral side of the eye imaginal discs. | Dihydroceramide desaturase promotes the formation of intraluminal vesicles and inhibits autophagy to increase exosome production. | iScience | 34877496 | FBrf0252065 |
| Here we Analyze the integrity of oxidative phosphorylation complexes in Drosophila flight muscles. | Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles. | STAR Protoc | 34977670 | FBrf0252164 |
2021
Dec 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We developed Bitbow, a highly efficient and easy-to-use multicolor labeling system suitable for neural lineage and morphological studies. Bitbow transgenic lines, with streamlined methods in sample prep, high-resolution imaging and analysis bring unprecedented resolution & scale. | Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains. | Front. Neural Circuits | 34744636 | FBrf0251755 |
| Optogenetic constructs for light-triggered cyclic AMP production. | PACmn for improved optogenetic control of intracellular cAMP. | BMC Biol. | 34663304 | FBrf0251641 |
| Adaptation of FRET-based kinase sensor for AMPK for use in fly. | AMPK adapts metabolism to developmental energy requirement during dendrite pruning in Drosophila. | Cell Rep. | 34788610 | FBrf0251912 |
| UAS-human ORF as well as UAS-Drosophila ORF. | A cross-species approach for the identification of Drosophila male sterility genes. | G3 (Bethesda) | 34849808 | FBrf0252010 |
| Creation of cell lines with attP cassette at either 25C6 or 99F8 on the 2nd or 3rd chromosomes respectively and the successful RMCE with an associated attB plasmid tool kit. | Generation of Drosophila attP containing cell lines using CRISPR-Cas9. | G3 (Bethesda) | 33963853 | FBrf0251996 |
| The paper describes the expression of CS Chrimson and TRPA in motoneurons and how to apply patterned stimulation to evoke activity-dependent synaptic plasticity at the NMJ. | A comparison of three different methods of eliciting rapid activity-dependent synaptic plasticity at the Drosophila NMJ. | PLoS ONE | 34847197 | FBrf0251887 |
| New transgenic shRNA to KD oskar mRNA that is substantially more effective during oogenesis that previously available reagents. | Knock down analysis reveals critical phases for specific oskar noncoding RNA functions during Drosophila oogenesis. | G3 (Bethesda) | 34586387 | FBrf0252056 |
| Two genes conferring resistance to specific drugs are expressed in opposite sexes by incorporating sex-specific introns disrupting the coding sequences of drug-resistance genes. When the transgenic strain is raised on food supplemented with the corresponding drug, only individuals of the desired sex emerge. | A drug-inducible sex-separation technique for insects. | Nat. Commun. | 32355156 | FBrf0245511 |
| CLADES is a technology for cell lineage studies based on CRISPR-Cas9 technology that uses a system of genetic switches to activate and inactivate reporter genes. | A programmable sequence of reporters for lineage analysis. | Nat. Neurosci. | 32719561 | FBrf0247972 |
Nov 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We developed a 3D-printed platform FlyVAB for intravital imaging strategy that enables the visualization of the Drosophila posterior midgut at a single cell level across the ventral abdomen cuticle. | Intravital imaging strategy FlyVAB reveals the dependence of Drosophila enteroblast differentiation on the local physiology. | Commun. Biol. | 34697396 | FBrf0251628 |
| An ethoscope extension that allows one to probe arousal threshold using qualitative stimuli. | Sensory processing during sleep in Drosophila melanogaster. | Nature | 34588694 | FBrf0251660 |
| Detailed 3C protocol suitable for Drosophila. | Investigation of the Basic Steps in the Chromosome Conformation Capture Procedure. | Front. Genet. | 34616432 | FBrf0251540 |
| Metabolic Flux-Balance-Network Models for Drosophila gut microbiome members. | Modeling Drosophila gut microbe interactions reveals metabolic interconnectivity. | iScience | 34712918 | FBrf0251721 |
| Model of macrophage infiltration into the inflamed brain, analysis of blood-brain barrier tightness in third instar larval and pupal brains. | Brain inflammation triggers macrophage invasion across the blood-brain barrier in Drosophila during pupal stages. | Sci. Adv. | 34705495 | FBrf0251689 |
| We implemented a method to feed flies with stable isotope-labeled methionine and coupled this approach with mass spectrometry analysis to determine the relative activities of different branches of methionine metabolism. | A genetic model of methionine restriction extends Drosophila health- and lifespan. | Proc. Natl. Acad. Sci. U.S.A. | 34588310 | FBrf0251481 |
| A method for cryopreserving Drosophila primordial germ cells and reviving original strains from the cryopreserved primordial germ cells has been developed. | Offspring production from cryopreserved primordial germ cells in Drosophila. | Commun. Biol. | 34621004 | FBrf0251547 |
| We identified a new allele of O-fut1 mutant and made new transgenes for pen-2 and activated Notch. | Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation. | Biol. Open | 34731235 | FBrf0251775 |
Oct 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We designed a new method to quantify shape. Our method is a semi-automatic measurement pipeline that can reliably identify curvatures and landmarks based on manually digitized contours of a 2D anatomical structure. | A morphological trait involved in reproductive isolation between Drosophila sister species is sensitive to temperature. | Ecol. Evol. | 34188829 | FBrf0249343 |
| Algorithm to analyse adult behaviours. | Drosophila females receive male substrate-borne signals through specific leg neurons during courtship. | Curr. Biol. | 34174209 | FBrf0251042 |
| We used a multivariate analysis of variance to identify relevant SNPs from a genome-wide association study of starvation resistance of DGRP flies after being fed four different diets. This approach yielded a dataset of novel diet-responsive candidate genes. | Genome-wide analysis in Drosophila reveals diet-by-gene interactions and uncovers diet-responsive genes. | G3 (Bethesda) | 34568906 | FBrf0251364 |
| Development of an optogenetic system that allows precise spatio-temporal control of Notch signalling using light. | Desensitisation of Notch signalling through dynamic adaptation in the nucleus. | EMBO J. | 34396565 | FBrf0251262 |
| This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in Drosophila. | Protocol for Drosophila sleep deprivation using single-chip board. | STAR Protoc | 34585161 | FBrf0251433 |
| Genome-wide astrocyte gene expression. | Circadian regulation of the Drosophila astrocyte transcriptome. | PLoS Genet. | 34543266 | FBrf0251441 |
| Single cell RNA sequencing dataset of the Drosophila brain following acute cocaine consumption. | The Drosophila brain on cocaine at single-cell resolution. | Genome Res. | 34035044 | FBrf0251482 |
| Consideration of D. melanogaster as a model insect to map genomic evolution in response to climate change. | Eco-genetics of desiccation resistance in Drosophila. | Biol. Rev. Camb. Philos. Soc. | 33754475 | FBrf0251240 |
| Additional dye for tracking food intake and food preference via Con-Ex. | Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila. | Sci. Rep. | 34625601 | FBrf0251557 |
| An explant based time-imaging system for studying olfactory circuit assembly. | Cellular bases of olfactory circuit assembly revealed by systematic time-lapse imaging. | Cell | 34551316 | FBrf0251446 |
Sep 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Novel microscopy approach to measure whole-brain neurodegeneration within the Drosophila brain. | A protocol to detect neurodegeneration in Drosophila melanogaster whole-brain mounts using advanced microscopy. | STAR Protoc | 34382016 | FBrf0250121 |
| In this article, we created a two-step approach with the main purpose of helping to assign TF to the regulation of specific genes, using Drosophila melanogaster as a case study. | Filtering of Data-Driven Gene Regulatory Networks Using Drosophila melanogaster as a Case Study. | Front. Genet. | 34394179 | FBrf0250139 |
| BiPOLES is a covalently linked version of the anion-channelrhodopsin GtACR2 and cation-channelrhodopsin Chrimson. It allows wavelength-dependent optogenetic silencing (blue light) or activation (red light) of the same neurons. BiPOLES is expressed under 20xUAS control. | BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons. | Nat. Commun. | 34312384 | FBrf0249792 |
| DNase-seq to identify regulatory regions in two distant Drosophila species (D. melanogaster and D. virilis), assessed evolutionary conservation and adaptive evolution of enhancers throughout multiple stages of embryogenesis to interrogate signs of positive or negative selection. | The hourglass model of evolutionary conservation during embryogenesis extends to developmental enhancers with signatures of positive selection. | Genome Res. | 34266978 | FBrf0250943 |
| This study provides a method to combine multiple studies in order to uncover genetic changes not possible to see within typical sample sizes. | Identification of conserved transcriptome features between humans and Drosophila in the aging brain utilizing machine learning on combined data from the NIH Sequence Read Archive. | PLoS ONE | 34379632 | FBrf0250159 |
| Quantification of bacteria on the cuticle surface by qPCR. | Ratio between Lactobacillus plantarum and Acetobacter pomorum on the surface of Drosophila melanogaster adult flies depends on cuticle melanisation. | BMC Res. Notes | 34496944 | FBrf0251023 |
| Improved light-inducible nuclear export system (iLEXY) - an optogenetic loss-of-function approach that triggers translocation of proteins from the nucleus to the cytoplasm. We present two versions of the system with fast or slow recovery nuclear kinetics for targeted proteins. | Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos. | Dev. Cell | 34363757 | FBrf0250332 |
| A novel method for interaction detection to compare the contact dynamics of macrophages in the Drosophila embryo. A framework called macrosight, which analyses the movement and interaction of migrating macrophages, including a segmentation and tracking algorithm. | Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection. | J Imaging | 34460738 | FBrf0250323 |
| Guinea pig anti-Neuropeptide F (NPF) antibody. | The sugar-responsive enteroendocrine neuropeptide F regulates lipid metabolism through glucagon-like and insulin-like hormones in Drosophila melanogaster. | Nat. Commun. | 34376687 | FBrf0250203 |
| NaNuTrap accelerates nuclear labelling in embryos by eliminating the lag associated with GFP maturation (when coupled with maternally deposited GFP). It also converts cytoplasmic GFP expressed in transgenic fly lines into a nuclear label. Works with the existing Gal4/UAS system. | NaNuTrap: a technique for in vivo cell nucleus labelling using nanobodies. | Development | 34328170 | FBrf0250933 |
Aug 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| This study describes how to use the SunTag system to image mRNA translation at single mRNA resolution in fixed and live embryos. | Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. | Development | 34308974 | FBrf0249555 |
| Quantitative proteomic profile of both fat body (third instar larva) and thoracic muscles (pharate adults) were generated here. Both wild type and Dp-depleted tissues were profiled by tandem-mass tag-mass spectrometry (TMT-MS). | E2F/Dp inactivation in fat body cells triggers systemic metabolic changes. | eLife | 34251339 | FBrf0249520 |
| Analysis software for live imaging of transcription in early embryos, and MATLAB machine learning analysis pipeline for modeling of Pol II pausing topology using live imaging data. | Quantitative imaging of transcription in living Drosophila embryos reveals the impact of core promoter motifs on promoter state dynamics. | Nat. Commun. | 34301936 | FBrf0249544 |
| The use of Voronoi diagrams to quantify the disorganization of a tissue (circumferential actin fibers of Drosophila's heart in this case). | Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage. | Sci. Rep. | 34315987 | FBrf0249633 |
| We show that light-field microscopy can be used for long-term high-speed 3D imaging with very low phototoxicity, which can be used to observe the embryo development at very low cost. | DiLFM: an artifact-suppressed and noise-robust light-field microscopy through dictionary learning. | Light Sci Appl | 34315860 | FBrf0249628 |
| Morphological and electrophysiological methods to analyse adult abdominal neuromuscular synapses during ageing. | Miniature neurotransmission is required to maintain Drosophila synaptic structures during ageing. | Nat. Commun. | 34285221 | FBrf0249540 |
| AnnoMiner is a flexible tool to annotate and integrate epigenetic as well as transcription factor occupancy data on the genome scale. It contains all high-quality ENCODE chromatin data that can be investigated with AnnoMiner. | AnnoMiner is a new web-tool to integrate epigenetics, transcription factor occupancy and transcriptomics data to predict transcriptional regulators. | Sci. Rep. | 34326396 | FBrf0249589 |
Jul 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We have developed new split fluorescent protein (FP) systems. Our split FP tagging approach allows us to study the proximity between and localization of multiple proteins endogenously expressed in essentially any cell type in Drosophila. | Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila. | Proc. Natl. Acad. Sci. U.S.A. | 34074768 | FBrf0249114 |
| Super resolution microscopy and single molecule localisation in adult Drosophila melanogaster brains. | Tracking Single Molecule Dynamics in the Adult Drosophila Brain. | eNeuro | 33875453 | FBrf0249175 |
| Dataset to cross-validate candidate genes with significant transcriptional response to temperature or variant showing non-random association with temperature. Use the dataset to focus on genes reported by several previous similar studies or on genes not previously reported. | False and true positives in arthropod thermal adaptation candidate gene lists. | Genetica | 33963492 | FBrf0249313 |
| Live imaging of chromatin in 3rd instar larval muscles. Chromatin marked by H2B-dsRed, nuclear envelope marked by Klar-GFP (driven by muscle driver), and active chromatin marked by mintobody-GFP reactive with H3K9ac. Nuclear volume and nucleolus labeled with Cherry-NLS. | Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization. | Sci. Adv. | 34078602 | FBrf0249133 |
| Discovery of novel, non-canonical cold shock domains within Unr and determination of their structures (PDB identifiers: 6Y6M, 6Y6E, 6Y4H, 6Y96). Redefining Unr-interactome (protein and RNA) by RIP-seq and quantitative mass spectrometry (PXD018115, PRJEB37467). | Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras. | Cell Rep. | 32697992 | FBrf0248824 |
| Active-zone Ca2+ imaging in defined brain dopamine neurons. | Presynaptic inhibition of dopamine neurons controls optimistic bias. | eLife | 34061730 | FBrf0249124 |
| Anti-JHAMT antibody (guinea pig). | A population of neurons that produce hugin and express the diuretic hormone 44 receptor gene projects to the corpora allata in Drosophila melanogaster. | Dev. Growth Differ. | 34021588 | FBrf0249406 |
| We combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patterns at a single-gene resolution in asymmetrically dividing cells in vivo. | Characterization of histone inheritance patterns in the Drosophila female germline. | EMBO Rep. | 34031963 | FBrf0249366 |
| New female-killing strains of Drosophila suzukii based on Tetracycline-off system were generated. | A transgenic female killing system for the genetic control of Drosophila suzukii. | Sci. Rep. | 34155227 | FBrf0249400 |
| Method to identify E3 ubiquitinated substrates in vivo. | The ubiquitin ligase Ariadne-1 regulates neurotransmitter release via ubiquitination of NSF. | J. Biol. Chem. | 33581113 | FBrf0249438 |
| Multiple OAMB antagonists and one OAMB agonist were identified in this paper. | A platform utilizing Drosophila ovulation for nonhormonal contraceptive screening. | Proc. Natl. Acad. Sci. U.S.A. | 34260376 | FBrf0249495 |
| Quantitative computational simulation of apical constrictions during ventral furrow formation. | Mechanical feedback and robustness of apical constrictions in Drosophila embryo ventral furrow formation. | PLoS Comput. Biol. | 34228708 | FBrf0249494 |
| Method to inflict blunt force injuries in third-instar larvae. | Effects of blunt force injuries in third-instar Drosophila larvae persist through metamorphosis and reduce adult lifespan. | MicroPubl Biol | 34278243 | FBrf0249473 |
| This one-step method generates, for any locus, a conditional knockout allele in Drosophila. The allele carries a bright fluorescent marker for easy screening and an attP landing site for subsequent genetic manipulations. | One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster. | STAR Protoc | 34095868 | FBrf0249159 |
Jun 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We developed a software to quantify the heartbeat parameters of intact larvae immobilized in a microfluidic device. Our software automatically detects the heart location in a video, extracts and detrends the heartbeat signal, and measures several heartbeat parameters. | Open access tool and microfluidic devices for phenotypic quantification of heart function of intact fruit fly and zebrafish larvae. | Comput. Biol. Med. | 33774273 | FBrf0248933 |
| Super resolution live cell microscopy using super resolution by optical photon reassignment (SoRa). | Actin-dependent membrane polarization reveals the mechanical nature of the neuroblast polarity cycle. | Cell Rep. | 34010656 | FBrf0249028 |
| UAS construct - mitochondria outer membrane GFP marker; Deep learning strategy for automatic mitochondria segmentation (https://github.com/fabda/Myofibril_paper) | Myofibril and mitochondria morphogenesis are coordinated by a mechanical feedback mechanism in muscle. | Nat. Commun. | 33828099 | FBrf0248612 |
| A new temporal conditioning behavioral paradigm with automated classification of behavior types and quantification of behavioral rhythms. | Drosophila acquires seconds-scale rhythmic behavior. | J. Exp. Biol. | 33795422 | FBrf0249000 |
| Development of an intersectional genetic luciferase reporter of dVGLUT expression specifically in dopamine neurons. | Vesicular glutamate transporter modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration. | Aging Cell | 33909313 | FBrf0249030 |
| Live imaging of translation in living drosophila embryos | Imaging translation dynamics in live embryos reveals spatial heterogeneities. | Science | 33927056 | FBrf0249049 |
| A new genome targeting technique to generate clean custom alleles that only have the desired mutation(s) is described in this paper. Plasmid vectors have been deposited to the DGRC, and tool flies generated in this study are available upon request. | Scarless engineering of the Drosophila genome near any site-specific integration site. | Genetics | 33772309 | FBrf0249020 |
| New injury method to study CNS regeneration in adults | A novel injury paradigm in the central nervous system of adult Drosophila: molecular, cellular and functional aspects. | Dis. Model Mech. | 34061177 | FBrf0249170 |
| SunTag method for imaging translation at single mRNA resolution in fixed and live embryos | Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. | Development | 33722899 | FBrf0249005 |
| We have generated UAS lines for Toll-2, Toll-6 and Toll-8 tagged with fluorescent markers for full-length receptors, receptors deleted of their extracellular domains and receptors deleted of their intracellular domains and made constructs to express Toll-8 in the early embryo. | Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry. | Dev. Cell | 33932333 | FBrf0249144 |
| A clustering algorithm designed for detecting conserved gene co-clusters among two species | Bipartite tight spectral clustering (BiTSC) algorithm for identifying conserved gene co-clusters in two species. | Bioinformatics | 32814973 | FBrf0249225 |
May 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Quantitative analysis of parkin-dependent mitophagy using confocal microscopy | 3D quantification of autophagy activation and autophagosome-to-mitochondria recruitment in a Drosophila model of Parkinson's disease. | STAR Protoc | 33851139 | FBrf0248763 |
| Seahorse experiments on cardiac tissue | Fat-body brummer lipase determines survival and cardiac function during starvation in Drosophila melanogaster. | iScience | 33889813 | FBrf0248791 |
| Development of proteomics of protein trafficking | Proteomics of protein trafficking by in vivo tissue-specific labeling. | Nat. Commun. | 33888706 | FBrf0248752 |
| Antibodies generated for AIF and DNase II | DNase II mediates a parthanatos-like developmental cell death pathway in Drosophila primordial germ cells. | Nat. Commun. | 33863891 | FBrf0248742 |
| A novel Drosophila model for mild traumatic brain injury for investigation of lifelong brain deficits and degeneration, it also has a new imaging-based approach for analyzing brain pathology in whole mount brains. | Repetitive mild head trauma induces activity mediated lifelong brain deficits in a novel Drosophila model. | Sci. Rep. | 33958652 | FBrf0248899 |
| We generated a roGFP-CAAX construct (for mammalian expression) that can be used to examine the redox-state on the plasma membrane. | Dihydroceramide desaturase regulates the compartmentalization of Rac1 for neuronal oxidative stress. | Cell Rep. | 33852856 | FBrf0248757 |
| A fluorogen activating protein neuropeptide (Dilp2-FAP) construct was used with membrane impermeant malachite green derivatives (MG-TCarb and MG-B-Tau) to detect neuropeptide release in the brain, specifically in LNv clock neurons | Temporally and spatially partitioned neuropeptide release from individual clock neurons. | Proc. Natl. Acad. Sci. U.S.A. | 33875606 | FBrf0248728 |
| Here we design a microfluidic device capable of ensuring the normal development of multiple fly embryos as well as achieving real-time temperature control and fast temperature switches for quantitative live imaging with a home-built two-photon microscope. | Quantifying Temperature Compensation of Bicoid Gradients with a Fast T-Tunable Microfluidic Device. | Biophys. J. | 32853562 | FBrf0248966 |
| We use whole-genome sequencing of Drosophila tissues (gut, head) to discover somatic genetic variation: 1- short-read Illumina sequencing of low-input DNA isolated from spontaneous clonal gut neoplasia, 2- long-read Oxford Nanopore (ONT) sequencing of DNA from pooled tissues. | Unraveling the features of somatic transposition in the Drosophila intestine. | EMBO J. | 33634906 | FBrf0248816 |
| The work uses the isotropic fractionator method with Drosophila and mosquito brains. This method allows counting the number of cells in a tissue. If can be used with an antibody or genetic labeling (GAL4/UAS or QF/QUAS) and be applied to counting sub-populations of a tissue. | The number of neurons in Drosophila and mosquito brains. | PLoS ONE | 33989293 | FBrf0248958 |
| GRAF plays a role in restricting the constriction of the actomyosin based contractile ring in cellularization. | Spatiotemporal recruitment of RhoGTPase protein GRAF inhibits actomyosin ring constriction in Drosophila cellularization. | eLife | 33835025 | FBrf0248856 |
Apr 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We have developed an automated imaging system constituted of a light sheet fluorescence microscope on a chip that allows analysis of Drosophila embryos in suspension that are fluxing in through a microfluidic chamber. | Automatic imaging of Drosophila embryos with light sheet fluorescence microscopy on chip. | J. Biophotonics | 33295053 | FBrf0248287 |
| Method to quantify epithelial barrier permeability in Drosophila imaginal discs using fluorescent dextran. The permeability of the experimental sample is compared to control samples that have a disrupted or non-functional barrier. Modified from Lamb et al. (1998). | Ecdysone regulates the Drosophila imaginal disc epithelial barrier, determining the length of regeneration checkpoint delay. | Development | 33658221 | FBrf0248455 |
| We developed PEGASUS_flies, which calculates whole gene significance scores from variant P-values that were output in a GWAS. Our platform works well for Drosophila/Drosophila Genetic Reference Panel (DGRP) lines. Available at: https://github.com/ramachandran-lab/PEGASUS_flies | Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila. | PLoS Genet. | 33735180 | FBrf0248437 |
| Hypoxia chamber with strictly controlled environmental parameters | Posthypoxic behavioral impairment and mortality of Drosophila melanogaster are associated with high temperatures, enhanced predeath activity and oxidative stress. | Exp. Mol. Med. | 33564101 | FBrf0248297 |
| We introduced DrosoX technique (Scitech Korea, Republic of Korea) for the quantitative analysis of food intake by individual flies. | Cucurbitacin B Suppresses Hyperglycemia Associated with a High Sugar Diet and Promotes Sleep in Drosophila melanogaster. | Mol. Cells | 33542166 | FBrf0248331 |
| The "resource" is an accurate phylogeny for the montium group of Drosophila | A phylogeny for the Drosophila montium species group: A model clade for comparative analyses. | Molec. Phylog. Evol. | 33387647 | FBrf0248412 |
| UAS-based transgenic lines for expressing genes from the SARS-CoV-2 virus that causes COVID-19 were generated. | Functional analysis of SARS-CoV-2 proteins in Drosophila identifies Orf6-induced pathogenic effects with Selinexor as an effective treatment. | Cell Biosci. | 33766136 | FBrf0248509 |
| Targated DamID of olfactory sensory neuron populations | Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants. | eLife | 33666172 | FBrf0248540 |
| pPWG-attB empty vector. A modified pPWG vector bearing the attB region inserted at a StuI site. It is compatible with Gateway system and can be used for random insertion in the genome (as a standard pPWG) but also in attB/P site specific integration ( phiC31 mediated). | Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins. | Dev. Cell | 33725482 | FBrf0248552 |
| Identification of mRNA targets of Ataxin2 (Atx2) in Drosophila brain by fusing it to the catalytic domain of adenosine deaminase (ADAR) enzyme. ADAR converts adenosine into inosine and subsequent RNA-Seq identifies these conversions (edits) as guanosines. | Antagonistic roles for Ataxin-2 structured and disordered domains in RNP condensation. | eLife | 33689682 | FBrf0248408 |
| Single molecule fluorescence in situ hybridisation in whole Drosophila brain. | Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. | eLife | 33724180 | FBrf0248505 |
| Novel method to compare 3D chromatin conformation data in Drosophila | Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning. | Nat. Genet. | 33795866 | FBrf0248615 |
| A lineage recording system termed "intMEMOIR" designed for in situ readout, thereby enabling simultaneous analysis of cell lineage, state, and spatial organization in the same tissue. | Imaging cell lineage with a synthetic digital recording system. | Science | 33833095 | FBrf0248662 |
Mar 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| We describe a fluorescent in situ hybridization protocol for the detection of endogenous Drosophila gut microbiome bacteria in different sample types (isolated bacteria, feces, larval and adult guts). We also added an automated analysis pipeline, which should be useful for others. | Visualization of endogenous gut bacteria in Drosophila melanogaster using fluorescence in situ hybridization. | PLoS ONE | 33606846 | FBrf0248171 |
| FreeClimber is an automated and high-throughput method for accurately and repeatably quantifying group climbing performance/climbing velocity/negative geotaxis in Drosophila. For more information, see the GitHub repository at: https://github.com/adamspierer/freeclimber | FreeClimber: automated quantification of climbing performance in Drosophila. | J. Exp. Biol. | 33188065 | FBrf0247781 |
| CLADES, a tool to analyze neuronal lineages in Drosophila. | A programmable sequence of reporters for lineage analysis. | Nat. Neurosci. | 32719561 | FBrf0247972 |
| The paper describes the use of Hedgehog signaling compenents: The signal Hedgehog and receptor Patched directed expression to model mutually exclusive computations in the context and highlights useful applications and tries to imagine the technology's potential. | A Turing machine for the Life Sciences. | D. I. S. | FBrf0248044 | |
| New antibody, new transgenic lines, new S2 cell stable cell lines. | Drosophila Tubulin-Specific Chaperone E Recruits Tubulin around Chromatin to Promote Mitotic Spindle Assembly. | Curr. Biol. | 33259793 | FBrf0248211 |
| In this manuscript it is reported a new setup to evaluate for the first time the release of neuroactive amines in Drosophila brain, by in vivo Fast-Scan Cyclic Voltammetry, while the fly is being exposed to odorants or an electric shock. | Study of the release of endogenous amines in Drosophila brain in vivo in response to stimuli linked to aversive olfactory conditioning. | J. Neurochem. | 32596813 | FBrf0248195 |
| Generation of novel transgenic flies expressing a monocistronic version of the human FUS gene (expresses only the FUS protein or only the altFUS protein), as well as the wild-type human FUS gene (expresses both proteins, FUS and altFUS). | The FUS gene is dual-coding with both proteins contributing to FUS-mediated toxicity. | EMBO Rep. | 33226175 | FBrf0248130 |
| Gene Drive element (split) located in specific Drosophila melanogaster genes. | Inherently confinable split-drive systems in Drosophila. | Nat. Commun. | 33674604 | FBrf0248266 |
| By using the previously defined matrisomes of Drosophila, we analyzed recorded genotype-to-phenotype relationships to define the Drosophila extracellular matrix phenome. | The extracellular matrix phenome across species. | Matrix Biol Plus | 33543035 | FBrf0247970 |
| This article indicates the maximum length of introgession segments that produce hybrid segmental offspring in heterozygosity, introgressing chromosomal segments of D. koepferae into a genetic background of D. buzzatii. | Evaluation of Drosophila chromosomal segments proposed by means of simulations of possessing hybrid sterility genes from reproductive isolation. | J. Genet. | 33622987 | FBrf0248240 |
Feb 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| This paper describes reagents (e.g. transgenic flies, plasmids, cloning protocols) to perform prime editing in Drosophila melanogaster. | Precise genome engineering in Drosophila using prime editing. | Proc. Natl. Acad. Sci. U.S.A. | 33443210 | FBrf0247772 |
| Metabolic flux analysis of lipids. | Lipidome-wide 13C flux analysis: a novel tool to estimate the turnover of lipids in organisms and cultures. | J. Lipid Res. | 31712250 | FBrf0247854 |
| Developmental scRNA-seq atlas of the Drosophila optic lobe. | Neuronal diversity and convergence in a visual system developmental atlas. | Nature | 33149298 | FBrf0247762 |
| Application of digital droplet PCR in gene expression study. | Genes regulating development and behavior exhibited altered expression in Drosophila melanogaster exposed to bisphenol A: use of real-time quantitative PCR (qRT-PCR) and droplet digital PCR (ddPCR) in genotoxicity study. | Environ. Sci. Pollut. Res. Int. | 33025430 | FBrf0247932 |
| The transgenic sexing strains of Drosophila suzukii based on the testis-specific fluorescent expression. | Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination. | Gene | 33346099 | FBrf0247834 |
| New model of flight exercise, new model of the Western Diet. | Preference and detrimental effects of high fat, sugar, and salt diet in wild-caught Drosophila simulans are reversed by flight exercise. | FASEB Bioadv | 33490883 | FBrf0247838 |
| Identification of neutral lipid-based secreted structures called microcarriers in the lumen of the male accessory gland, which can be stained by neutral lipid dyes like LipidTox and Nile Red. | Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid. | Proc. Natl. Acad. Sci. U.S.A. | 33495334 | FBrf0247869 |
| Long-term in vivo imaging of Drosophila giant larvae tumor migration and invasion using SiMView microscopy. | The level of oncogenic Ras determines the malignant transformation of Lkb1 mutant tissue in vivo. | Commun. Biol. | 33514834 | FBrf0247944 |
| Methods to examine the musculature of the Drosophila larva. These methods include analysis of the muscle sarcomeres, particularly in the same larva throughout the different larval instars. This work contains methods to muscle structural changes to muscle functional changes. | Analyzing muscle structure and function throughout the larval instars in live Drosophila. | STAR Protoc | 33532738 | FBrf0247996 |
| We verified that Drosophila S2R+ adapted to grow in serum-free media is amenable to RNAi interference-mediated gene knockdown. | Serum-free adapted Drosophila S2R+ line is amenable to RNA interference. | Micropub Biol | 33537561 | FBrf0248021 |
| Single cell genomics of testes. | Dynamic sex chromosome expression in Drosophila male germ cells. | Nat. Commun. | 33563972 | FBrf0248088 |
| New reporter line and new null allele were generated in this paper. | The Krüppel-like factor Cabut has cell cycle regulatory properties similar to E2F1. | Proc. Natl. Acad. Sci. U.S.A. | 33558234 | FBrf0248123 |
| Split-GAL4 lines labeling specific cell types. | Neural circuit mechanisms of sexual receptivity in Drosophila females. | Nature | 33239786 | FBrf0247919 |
| A translational fidelity reporter (UAS-Fluc-STOP-Nluc) - Drosophila mutant strains (RPS23-R67K, RPS26-KO). | Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. | Nat. Cell Biol. | 33495632 | FBrf0248082 |
| The modified PER assay can deliver stimuli not only for taste, but also for other senses. We made a line that can express the hair plate in legs. | Multisensory interactions regulate feeding behavior in Drosophila. | Proc. Natl. Acad. Sci. U.S.A. | 33558226 | FBrf0248109 |
| UAS-MagIC, fluorescent Mg2+ reporter transgene. | Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory. | eLife | 33242000 | FBrf0247921 |
| Mathematical algorithm for cytoneme/Tumor microtube expansion. | Modeling invasion patterns in the glioblastoma battlefield. | PLoS Comput. Biol. | 33513131 | FBrf0248065 |
| We developed a new technique called mosaic analysis by gRNA-induced crossing-over (MAGIC) for mosaic analysis with unmodified genomes. We developed the MAGIC toolkit for chromosomal arm 2L. | Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes. | PLoS Biol. | 33444322 | FBrf0247818 |
Jan 2021
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Human genes expressed in breast cancer patients are screened for their ability to aberrate development of the Drosophila eye. Transgenic construct UAS-hRPS12 was described in this paper. | HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis. | Sci. Rep. | 33273532 | FBrf0247452 |
| Python-based code used to quantify object-to-object distances; e.g., mRNA to centrosomes. | centrocortin RNA localization to centrosomes is regulated by FMRP and facilitates error-free mitosis. | J. Cell Biol. | 33196763 | FBrf0247305 |
| A single cell atlas of the proximal wing disc was built by single cell RNAseq. The myoblast, tracheal and epithelial cells associated with third instar larval wing disc were mapped. Diverse cell types along with specific markers were identified. | A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence in Drosophila. | EMBO Rep. | 32815271 | FBrf0247435 |
| Expansion microscopy for larval tissue. | Recruitment of BAF to the nuclear envelope couples the LINC complex to endoreplication. | Development | 33168584 | FBrf0247504 |
| Several new fly lines. In particular: - a GFP-tagged FoxP gene - a null allele - a conditional UAS-gRNA-based knock-out line. | Identification of FoxP circuits involved in locomotion and object fixation in Drosophila. | Open Biol | 33321059 | FBrf0247550 |
| Nuclear localization signal (NLS) that isolated from D. suzukii transformer gene. | Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides. | Insect Biochem. Mol. Biol. | 31978587 | FBrf0245063 |
| piggyBac constructs that express DeRed at embryonic stage. | Identification and characterization of four Drosophila suzukii cellularization genes and their promoters. | BMC Genet. | 33339500 | FBrf0247583 |
| The protocol describes preparation of adult axenic Drosophila before monitoring their behavior in a two-choice feeding assay, where flies are confronted with an axenic versus a dead or alive bacteria-contaminated feeding solution. | Protocol for a Binary Choice Feeding Assay Using Adult, Axenic Drosophila. | STAR Protoc | 33377013 | FBrf0247622 |
| CRISPR dual-gRNA transgenic targeting phosphoinositide signaling genes. | A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling. | eLife | 33320085 | FBrf0247664 |
| Tissue- and time-resolved chromatin accessibility during Drosophila embryogenesis. | Lineage-Resolved Enhancer and Promoter Usage during a Time Course of Embryogenesis. | Dev. Cell | 33171098 | FBrf0247494 |
| We apply deep learning on thousands of mRNA datasets to examine the genetic regulatory code controlling mRNA abundance in model organisms including Drosophila melanogaster, and show that in all organisms mRNA abundance can be predicted directly from DNA sequence. | Deep learning suggests that gene expression is encoded in all parts of a co-evolving interacting gene regulatory structure. | Nat. Commun. | 33262328 | FBrf0247517 |
| UAS-driven regulatable fluorescent secretory cargo.] | ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. | Mol. Biol. Cell | 33112725 | FBrf0247546 |
| 1. S2 cell culture and luciferase reporter assay; 2. AGO1 immunoprecipitation (AGO1 IP); 3. Immunofluorescence experiments. | Regulatory mechanism of daily sleep by miR-276a. | FASEB J. | 33337563 | FBrf0247672 |
| Resource: website for downloading aligned genome sequences from Drosophila pseudoobscura species group. | PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup. | Fly | 33319644 | FBrf0247745 |
| S2 cell culture and luciferase reporter assay; AGO1 immunoprecipitation. | Regulation of circadian behavioural output via clock-responsive miR-276b. | Insect Mol. Biol. | 33131172 | FBrf0247752 |
| Superresolution imaging of glutamate receptors were obtained with structured illumination microscopy (SIM). | Postsynaptic cAMP signalling regulates the antagonistic balance of Drosophila glutamate receptor subtypes. | Development | 33234716 | FBrf0247665 |
| Characterization of novel chromosomal structures in Drosophila embryonic cells, by combining 3D structured illumination microscopy (3D-SIM) and DAPI or FISH staining with a X Oligopaint probe (observation in a haploid context in sorted male embryos using a Y-GFP line). Ext. Fig.6. | Regulation of single-cell genome organization into TADs and chromatin nanodomains. | Nat. Genet. | 33077913 | FBrf0247675 |
| The methods paper describes detailed procedure for X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs to measure elemental distribution. Our report on the elemental distributions themselves was published in Hu et al. iScience 2020. | X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs. | STAR Protoc | 33437967 | FBrf0247770 |
2020
Dec 2020
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Analysis of Gal4 expression patterns of commonly used Gal4 drivers in adult females. | Analysis of Gal4 Expression Patterns in Adult Drosophila Females. | G3 (Bethesda) | 32917721 | FBrf0247159 |
| We defined the origins of repeat-derived RNAs and their specific chromatin locations in Drosophila S2 cells using the global RNAâDNA interaction sequencing method (GRID-seq). | Active retrotransposons help maintain pericentromeric heterochromatin required for faithful cell division. | Genome Res. | 33060173 | FBrf0247182 |
| We have developed a novel automated Drosophila life cycle multi-phase transition (pupariation, eclosion, and death) timing detection and measurement system with high accuracy and high temporal resolution. | The Drosophila Individual Activity Monitoring and Detection System (DIAMonDS). | eLife | 33168136 | FBrf0247192 |
| A hydrogen peroxide probe was detected in vivo through a burnt fluorescent probe (TCFB, 2-Dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran) (Sedgwick et al., 2017). | Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation. | Cell Rep. | 33176146 | FBrf0247218 |
| The binary expression of different pro-apoptotic genes using 2A peptides. | Functional characterization of the Drosophila suzukii pro-apoptotic genes reaper, head involution defective and grim. | Apoptosis | 33113043 | FBrf0247316 |
| Improved pipeline for CRISPR mediated genome editing in Drosophila species. Generation of new vector to make donor plasmids for homology-directed repair. | A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila. | Fly | 33016195 | FBrf0247326 |
| We describe a novel high throughput mass spectrometry approach to identifying species and sex by their metabolites. We show it works invertebrates generally, and can accurately distinguish closely related Drosophila (e.g. melanogaster and simulans) with potential for mass scoring. | The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species-a potential new tool in entomology. | Open Biol. | 33234068 | FBrf0247327 |
| Here we introduce TEchim, a new software that extracts chimeric sequencing reads that span transposon-gene breakpoints. In addition, we introduce scHardyWeinberg, a new approach to quantify co-expression levels in high-throughput single-cell sequencing data. | Transposon expression in the Drosophila brain is driven by neighboring genes and diversifies the neural transcriptome. | Genome Res. | 32973040 | FBrf0247119 |
| Single-cell RNA-sequencing dataset of the adult Drosophila ovary containing transcriptome profiles for all major ovarian cell types. | A single-cell atlas and lineage analysis of the adult Drosophila ovary. | Nat. Commun. | 33159074 | FBrf0247148 |
| We developed an optimized version of the bright and fast-maturing fluorescent protein mNeonGreen to serve as a real-time, quantitative reporter of gene/enhancer expression. | A sensitive mNeonGreen reporter system to measure transcriptional dynamics in Drosophila development. | Commun. Biol. | 33184447 | FBrf0247254 |
| DRB treatment followed by Pol II ChIP. | Evidence of slightly increased Pol II pausing in UPF1-depleted Drosophila melanogaster cells. | Micropub Biol | 33274326 | FBrf0247413 |
Nov 2020
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Antibody. | AANAT1 functions in astrocytes to regulate sleep homeostasis. | eLife | 32955431 | FBrf0246918 |
| Endogenous locus was edited with a two-step CRISPR/Cas9-mediated replacement. | Humanization of Drosophila Gαo to Model GNAO1 Paediatric Encephalopathies. | Biomedicines | 33036271 | FBrf0246897 |
| We optimized, tested, and introduced a new method of automated count of Drosophila adults using the SeedCounter mobile application. The application is available on all mobile devices based on the Android system, and it does not require any additional equipment. | Quantifying Drosophila adults with the use of a smartphone. | Biol. Open | 32917765 | FBrf0246907 |
| We introduce a developmental 96-well plate assay to use individual Drosophila larvae as tool for metabolic studies. | How to use the development of individual Drosophila larvae as a metabolic sensor. | J. Insect Physiol. | 32783958 | FBrf0246919 |
| Novel method to compare 3D chromatin conformation data in Drosophila. | CHESS enables quantitative comparison of chromatin contact data and automatic feature extraction. | Nat. Genet. | 33077914 | FBrf0247073 |
| Comprehensive calcium imaging from all 2,000 Kenyon cells in the mushroom body at single cell resolution. | Synthesis of Conserved Odor Object Representations in a Random, Divergent-Convergent Network. | Neuron | 32814018 | FBrf0247109 |
| Dominant negative form of IP3R protein. It has three mutated amino acids which prevent ligand binding. IP3R being tetramer, requires IP3 binding to all monomers. Hence presence of even single IP3R-DN monomer in tetramer can render whole IP3R protein complex functionless. | Modulation of flight and feeding behaviours requires presynaptic IP3Rs in dopaminergic neurons. | eLife | 33155978 | FBrf0247160 |
| Use of Hydrogel for live-imaging of Drosophila ovaries. | Collective Cell Sorting Requires Contractile Cortical Waves in Germline Cells. | Curr. Biol. | 32916115 | FBrf0247169 |
| This paper uses fast-scan cyclic voltammetry at a carbon-fiber microelectrode to make real-time measurement of stimulated endogenous dopamine release in the mushroom body of adult Drosophila brains using various stimulation techniques. | Real-Time Measurement of Stimulated Dopamine Release in Compartments of the Adult Drosophila melanogaster Mushroom Body. | Anal. Chem. | 33048531 | FBrf0247175 |
| New measurements of Position weight Matrices (PWMs) Dinucleotide Position weight Matrices (DPWMs) for 13 Drosophila transcription factors and their DNA shape analysis for all shape features. | Transcription Factor Binding Affinities and DNA Shape Readout. | iScience | 33163946 | FBrf0247184 |
| Sequence, features, and schematic of the pWattB-nlacZ reporter construct vector. | Three distinct mechanisms, Notch instructive, permissive, and independent, regulate the expression of two different pericardial genes to specify cardiac cell subtypes. | PLoS ONE. | 33108408 | FBrf0247019 |
| A new method for immobilizing explanted Drosophila tissue in agarose for use in long-term live imaging. | An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues. | Front. Cell Dev. Biol. | 33117817 | FBrf0247101 |
| Using new fast-scan cyclic voltammetry serotonin specific waveforms for in vivo detection of serotonin in Drosophila tissue. | Improving serotonin fast-scan cyclic voltammetry detection: new waveforms to reduce electrode fouling. | Analyst | 32955048 | FBrf0247149 |
| We have developed a new protocol to indcue cell cycle arrest in early embryos, and deposited RNA-seq data for WT embryos and embryos arrested in cycle 12 for differernt amount of time. | Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio. | PLoS Biol. | 33090988 | FBrf0247179 |
| Analysis of the chronometabolome including lipids. | Loss of function in the Drosophila clock gene period results in altered intermediary lipid metabolism and increased susceptibility to starvation. | Cell. Molec. Life Sci. | 31960114 | FBrf0247228 |
| Transgenic Targeted DamID construct for the gene polycomb-like (Pcl) under the control of a UAS. | Persistent epigenetic reprogramming of sweet taste by diet. | Sci. Adv. | 33177090 | FBrf0247230 |
Oct 2020
| Author description of technical advance | Title | Journal | PubMed ID | FBrf number |
|---|---|---|---|---|
| Small molecules called "HACs" alter normal autoprocessing activity of hedgehog precursor protein. Instead of reacting with cholesterol (cholesterolysis), the HACs alter hedgehog precursor autoprocessing toward a side reaction with water (hydrolysis). | Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis. | Biochemistry | 32013401 | FBrf0246799 |
| This paper shows how computational network analyses based on protein-protein interactions can be used to augment genetic screens to identify genes involved in essential developmental processes. The code are deposited in GitHub https://github.com/extavourlab/hpo_ova_eggL_screen. | Topology-driven protein-protein interaction network analysis detects genetic sub-networks regulating reproductive capacity. | eLife | 32901612 | FBrf0246922 |
| PhotoGal4 is a phytochrome B-based optogenetic switch for fine tuned spatiotemporal control of gene expression in Drosophila explants. PhotoGal4 activates and deactivates gene expression upon red- or far-red-light irradiation. | PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants. | iScience | 32652492 | FBrf0246250 |
| We developed a unique visual stimulation setup to probe spectral, spatial, & temporal properties using neural activity imaging. Custom optical filters enabled simultaneous two-photon imaging & visual stimulation over a wide range of hues without compromising the temporal rates. | A novel setup for simultaneous two-photon functional imaging and precise spectral and spatial visual stimulation in Drosophila. | Sci. Rep. | 32973185 | FBrf0246808 |
| New white gene mutant strains were generated in Drosophila suzukii and can be used as resources to study the corresponding phenotype. | CRISPR/Cas9 mediated disruption of the white gene leads to pigmentation deficiency and copulation failure in Drosophila suzukii. | J. Insect Physiol. | 32745561 | FBrf0246889 |
| This protocol allows generation of homozygous mutant cell lines using an insertion cassette which autocatalytically generates insertion mutations in all alleles. Knockout cells generated using this method can be directly identified by PCR without a need for DNA sequencing. | CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells. | Nat. Protoc. | 32958931 | FBrf0246845 |
| Drosophila melanogaster line infected only with galbut virus (FoCo-17 line 30). | Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission. | J. Virol. | 32759315 | FBrf0246864 |
| The isobaric tags for relative and absolute quantification (iTRAQ) technology was chosen to sequence the proteomes of Drosophila. | Comparative proteomics analysis of dietary restriction in Drosophila. | PLoS ONE | 33064752 | FBrf0246967 |