FlyBase:Antibodies
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Review antibodies tested in Drosophila.
pAbmAbs, a self-described Tripadvisor for antibodies, is a nonprofit resource to help researchers identify antibodies that work well, or do not work well, for their particular application by cataloguing both positive and negative community-submitted antibody reviews. FlyBase is working with pAbmAbs in an effort to increase the number of reviews submitted by Drosophila researchers and thereby increase pAbmAbs’ usefulness to the fly community. Submitting antibody reviews is easy and takes only a few minutes; we’ve even compiled some guidelines for you below. For your convenience, you may fill in this spreadsheet and email it to Simon at pAbmAbs for 'concierge' entry into the pAbmAbs database. Alternatively, you may go to the pAbmAbs website and enter antibody reviews individually yourself.
Suggested details to include in antibody reviews:
- Antibody name
- Source/Vendor
- Catalog number
- Original antigen
- Drosophila target/ortholog
- Assay
- Tissue/Cell line
- Antibody dilution/concentration
- Important experimental details (e.g. fixation conditions)
- Reference, if published
Please note that all submitted reviews are screened by pAbmAbs so there may be a delay between submission and appearance on the website.
Metasearches for antibodies.
| Resource |
|---|
| BioCompare Antibody Search Tool |
| BenchSci AI Literature Screen for Antibodies |
Links to collections of Drosophila-specific antibodies.
| Resource | Author/Source |
|---|---|
| Abbiotec | San Diego, CA, USA |
| Abcam | Cambridge, MA, USA |
| Acris Antibodies | San Diego, CA, USA Herford, Germany |
| Active Motif | Carlsbad, CA, USA International Locations |
| Antibodypedia | Vancouver, BC, Canada |
| Antibody Registry | RINL Resource Identification Initiative |
| Antibody Validation Database | University of California San Diego San Diego, CA, USA |
| Biorbyt | Cambridge, UK |
| Cell Signaling Technology | Danvers, MA, USA International Locations |
| Creative Biolabs Modelorg Antibody | Shirley, NY, USA |
| Developmental Studies Hybridoma Bank (DSHB) | University of Iowa Iowa City, IA, USA |
| FabGennix | Frisco, TX, USA |
| GeneTex | Irvine, CA, USA International Locations |
| MilliporeSigma | International Locations |
| Novus | Littleton, CO, USA |
| ProSci | El Cajon, CA, USA |
| R&D Systems | Minneapolis, MN, USA |
| R&D Systems | Minneapolis, MN, USA |
| Santa Cruz Biotechnology | Dallas, Texas, USA |
| Sigma Aldrich (additional link) | St. Louis, MO, USA International Locations |
| Thermo Fisher | Waltham, MA, USA |
| US Biological | International Locations |
Links to vendors whose collections include Drosophila-specific antibodies.
The table below contains non-direct Drosophila antibody links; additional searching is required from the vendor website.
| Resource | Author/Source |
|---|---|
| Abgent | San Diego, CA, USA Suzhou city, Jiangsu Province, China |
| antibodies-online | International Locations |
| Enzo Life Sciences | Farmingdale, NY, USA |
| LifeSpan BioSciences | Seattle, WA, USA |
Lab-generated antibodies in Gene Reports
Antibodies generated by research laboratories for specific proteins are listed in their respective Gene Report under "Stocks and Reagents" → "Antibody Information".
For example, the following screenshot is taken from the Notch gene report:
If no antibodies exist or they have not yet been curated, this section will be empty.
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