FlyBase:Drosophila Material Resources

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Genomic Clones

BAC Genomic clones and Filters

  • One BAC clone library made from DNA of the same y1 ; cn1 bw1 sp1 stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
  • BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
  • Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
  • For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org

P1 Genomic Clones

  • As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
  • For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.

Cosmid Genomic Clones and Filters

The members of the European Drosophila Genome mapping consortium are:

  • F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
    Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
  • D.M. Glover and R.D.C. Saunders,
    Department of Biochemistry, University of Dundee, Dundee, Scotland.
  • M. Ashburner,
    Department of Genetics, University of Cambridge, Cambridge, England.
  • J. Modolell,
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
    with initial funding from the Fondation Schlumberger.

Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.

Contact:

Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm

cDNA (EST Clones and Libraries)

Arrays and Primers

  • Affymetrix
    The Affymetrix GeneChip Drosophila Genome Array for gene expression analysis based on Release 3.1 annotations can be obtained at http://www.affymetrix.com/products/arrays/specific/fly_2.affx

    The Affymetrix Drosophila Tiling 2.0R Array based on Genome Release 5 with comprehensive genome coverage at 38bp resolution can be obtained here: http://www.affymetrix.com/products/arrays/specific/drosophila_tiling2_0r.affx
  • Drosophila Genomics Resource Center (DGRC)
    Produces and distributes DNA microarray slides. More information is available at http://dgrc.cgb.indiana.edu/
  • FlyChip
    FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/
  • FlyPrimerBank
    An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at www.flyrnai.org/flyprimerbank
  • International Drosophila Array Consortium (INDAC)
    An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at http://www.indac.net/
  • UK Drosophila Affymetrix Microarray Service
    A publicly funded microarray service for the UK Drosophila research community. More information is available at http://www.mblab.gla.ac.uk/igf/

Stocks

Expression Vectors

  • Drosophila Gateway Vectors
    A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC
  • ExpreS2ion Biotechnologies
    ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. ExpreS2ion Biotechnologies

Cell Lines and Hybridomas

  • Drosophila Genomics Resource Center (DGRC)
    Collects and distributes cell lines. More information is available at http://dgrc.cgb.indiana.edu/
  • Developmental Studies Hybridoma Bank
    The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at http://dshb.biology.uiowa.edu
  • ATCC
    The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at http://www.atcc.org/

Antibodies

Transgenic, Targeted Mutation, and Other Services

  • Aktogen behaviour genetic services
    Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. http://www.aktogen.com/
  • BestGene Transgenic Service
    The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at http://www.thebestgene.com/
  • C-CAMP Centre for Cellular and Molecular Platforms
    A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at http://ccamp.res.in/fly-facility.html
  • Drosophila TILLING Project
    The goal of Fly-TILL is to rapidly deliver to the Drosophila community EMS-induced mutations in requested genes via high-throughput reverse genetic screens. More information is available at http://tilling.fhcrc.org/fly/Welcome_to_Fly-TILL.html
  • Duke University Non-Mammalian Model Systems Flyshop
    The Duke University Non-Mammalian Model Systems Flyshop provides P-element transformation, dsRNA injection, embryo sorting, and inverse PCR services. More information is available at http://www.biology.duke.edu/model-system
  • Fly Facility
    Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at http://www.fly-facility.com/
  • Genetic Services
    Genetic Services provides the Drosophila community with services including transgenic production, injection work, genetic screening, stock creation, construct production and consulting. More information is available at http://www.geneticservices.com
  • GenetiCorps Custom Injection Services
    GenetiCorps offers high quality fly injection services to the Drosophila research community. For more information, please visit http://www.geneticorps.com/
  • Genetivision, Inc.
    Genetivision offers a transgenic fly service for both delta 2-3 transposase-mediated P-element transgenesis and Phi-C31 mediated site-specific integration. More information is available at http://www.genetivision.com/
  • Model Biosystems Drosophila Stock Center
    The stock center in Bangalore offers a stock maintenance service at a very reasonable price to the Drosophila researchers all over the world http://www.modelbiosystems.com
  • Rainbow Transgenic Flies
    Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at http://www.rainbowgene.com/
  • Transgenic Drosophila Fly Core
    The Cutaneous Biology Research Center (CRBC) Fly Core at Massachusetts General Hospital/Harvard Medical School provides microinjection services to create transgenic Drosophila fly strains. More information is available at http://www.flycore.com
  • Union Biometrica
    COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at http://www.unionbio.com/
  • VANEDIS Drosophila injection service
    The VANEDIS Drosophila injection service injects embryos with your construct of interest. More information is available at http://www.vanedis.no

RNA

Clontech
Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2

RNAi

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