Difference between revisions of "FlyBase:Antibodies"
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FlyBase is working with pAbmAbs, a nonprofit resource to help researchers identify antibodies that work well or do not work well for their particular experiment by cataloguing both positive and negative community-submitted antibody reviews. In an effort to increase the number of reviews submitted by Drosophila researchers and thereby increase pAbmAbs' usefulness to the fly community, we respectfully request that you and your colleagues consider submitting antibody reviews based on your extensive experience. For your convenience, you may fill in this [spreadsheet] and email it to [mailto:molgaard@pabmabs.com Simon at pAbmAbs] for 'concierge' entry into the pAbmAbs database. Alternatively, you may go to the [http://pabmabs.com/ pAbmAbs website] and enter antibody reviews individually yourself. | FlyBase is working with pAbmAbs, a nonprofit resource to help researchers identify antibodies that work well or do not work well for their particular experiment by cataloguing both positive and negative community-submitted antibody reviews. In an effort to increase the number of reviews submitted by Drosophila researchers and thereby increase pAbmAbs' usefulness to the fly community, we respectfully request that you and your colleagues consider submitting antibody reviews based on your extensive experience. For your convenience, you may fill in this [spreadsheet] and email it to [mailto:molgaard@pabmabs.com Simon at pAbmAbs] for 'concierge' entry into the pAbmAbs database. Alternatively, you may go to the [http://pabmabs.com/ pAbmAbs website] and enter antibody reviews individually yourself. | ||
| + | |||
| + | Suggested details to include in antibody reviews: | ||
| + | *Antibody name | ||
| + | *Source/Vendor | ||
| + | *Catalog number | ||
| + | *Original antigen | ||
| + | *Drosophila target/ortholog | ||
| + | *Assay | ||
| + | *Tissue/Cell line | ||
| + | *Antibody dilution/concentration | ||
| + | *Important experimental details (e.g. fixation conditions) | ||
| + | *Reference, if published | ||
| + | |||
| + | Please note that all submitted reviews are screened by pAbmAbs so there may be a delay between submission and appearance on the website. | ||
=Links to collections of <em>Drosophila</em>-specific antibodies.= | =Links to collections of <em>Drosophila</em>-specific antibodies.= | ||
Revision as of 20:27, 23 September 2020
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Review antibodies tested in Drosophila.
FlyBase is working with pAbmAbs, a nonprofit resource to help researchers identify antibodies that work well or do not work well for their particular experiment by cataloguing both positive and negative community-submitted antibody reviews. In an effort to increase the number of reviews submitted by Drosophila researchers and thereby increase pAbmAbs' usefulness to the fly community, we respectfully request that you and your colleagues consider submitting antibody reviews based on your extensive experience. For your convenience, you may fill in this [spreadsheet] and email it to Simon at pAbmAbs for 'concierge' entry into the pAbmAbs database. Alternatively, you may go to the pAbmAbs website and enter antibody reviews individually yourself.
Suggested details to include in antibody reviews:
- Antibody name
- Source/Vendor
- Catalog number
- Original antigen
- Drosophila target/ortholog
- Assay
- Tissue/Cell line
- Antibody dilution/concentration
- Important experimental details (e.g. fixation conditions)
- Reference, if published
Please note that all submitted reviews are screened by pAbmAbs so there may be a delay between submission and appearance on the website.
Links to collections of Drosophila-specific antibodies.
| Resource | Author/Source |
|---|---|
| Abbiotec | San Diego, CA, USA |
| Abcam | Cambridge, MA, USA |
| Acris Antibodies | San Diego, CA, USA Herford, Germany |
| Active Motif | Carlsbad, CA, USA International Locations |
| Antibodypedia | Vancouver, BC, Canada |
| Antibody Registry | RINL Resource Identification Initiative |
| Antibody Validation Database | University of California San Diego San Diego, CA, USA |
| Biorbyt | Cambridge, UK |
| Cell Signaling Technology | Danvers, MA, USA International Locations |
| Developmental Studies Hybridoma Bank (DSHB) | University of Iowa Iowa City, IA, USA |
| FabGennix | Frisco, TX, USA |
| GeneTex | Irvine, CA, USA International Locations |
| MilliporeSigma | International Locations |
| Novus | Littleton, CO, USA |
| ProSci | Poway, CA, USA |
| R&D Systems | Minneapolis, MN, USA |
| Santa Cruz Biotechnology | Dallas, Texas, USA |
| Sigma Aldrich (additional link) | St. Louis, MO, USA International Locations |
| US Biological | International Locations |
Links to vendors whose collections include Drosophila-specific antibodies.
The table below contains non-direct Drosophila antibody links; additional searching is required from the vendor website.
| Resource | Author/Source |
|---|---|
| Abgent | San Diego, CA, USA Suzhou city, Jiangsu Province, China |
| antibodies-online | International Locations |
| Enzo Life Sciences | Farmingdale, NY, USA |
| LifeSpan BioSciences | Seattle, WA, USA |
| Thermo Fisher | Waltham, MA, USA |
Lab-generated antibodies in Gene Reports
Antibodies generated by research laboratories for specific proteins are listed in their respective Gene Report under "Stocks and Reagents" → "Antibody Information".
For example, the following screenshot is taken from the Notch gene report:
If no antibodies exist or they have not yet been curated, this section will be empty.
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