FlyBase:Drosophila Material Resources

Genomic Clones

BAC Genomic clones and Filters

• The RPCI-98 Drosophila melanogaster BAC Library (17,540 clones in pBACe3.6; average clone size is 160-Kb), which was made from DNA of the same y¹ ; cn¹ bw¹ sp¹ stock as that used for the Berkeley Drosophila Genome Project P1 clones, is available from BACPAC Resources. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
• Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P[acman]-CmR-BW vector, a special adaptation of P[acman], are also available from P(acman) Resources.
• For information and resources related to the P[acman] recombineering technique see: P(acman) Resources

Fosmid CDS-GFP-tagged clones

• The Drosophila TransgeneOme Fosmid Library (a genome-scale collection of ~10,000 validated clones covering ~9600 genes (73% of the Drosophila proteome)), is a comprehensive genome-wide library of C-terminally tagged proteins within genomic fosmid constructs. The tagging cassette comprises affinity tags, protease sites and GFP suitable for protein localisation and complex purification studies. The complete set or chosen subsets are available from Source BioScience. Transgenic lines have been generated for about 900 of these clones and are available at Vienna Drosophila Resource Center (VDRC).

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.

Contact:

Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources for erecta, pseudoobscura ,virilis, and willistoni.

cDNA (EST Clones and Libraries)

• Drosophila Genomics Resource Center (DGRC)
  Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. Collections include BDGP Gold, BDGP Tagged ORF, Extracellular Domain, FlyBi ORFeome, BDGP DGC, BDGP EST, BDGP ORF, iPCR(IP), Testis cDNA, CuraGen, and Drosophila Species ESTs/cDNAs. More information is available at DGRC.
• addgene
  Addgene helps labs archive and share their plasmids that have appeared in publications.
  addgene
• Berkeley Drosophila Genome Project
  Information on cDNA availability can also be found here BDGP Resources.
• Brian Oliver Laboratory
  Testis cDNA clones and/or filters constructed in Brian Oliver's laboratory at the NIH are available at Source BioScience in the UK or at the DGRC in the U.S.
• cDNA library aliquots
  Aliquots from a number of the BDGP cDNA libraries are available from the labs on this list.

Arrays and Primers

• Affymetrix
  The Affymetrix GeneChip Drosophila Genome 2.0 Array for gene expression analysis based on Release 3.1 annotations can be obtained at Genome 2.0 Array.
  The Affymetrix GeneChip Drosophila Tiling 2.0R Array based on FlyBase Release 5 with comprehensive genome coverage at 38bp resolution can be obtained at Tiling 2.0R Array.
• Drosophila Genomics Resource Center (DGRC)
  No longer produces and distributes DNA microarray slides but maintains a microarray support archive. See DGRC.
• FlyChip
  FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at FlyChip.
• International Drosophila Array Consortium (INDAC)
  An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at FlyChip_long_oligonucleotide_003 (FL003) - INDAC.
• UK Drosophila Affymetrix Microarray Service
  A publicly funded microarray service for the UK Drosophila research community. More information is available at FlyChip Affymetrix
• FlyPrimerBank
  An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at DRSC FlyPrimer Bank

Stocks

• Bloomington Stock Center
  Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at BDSC
• CRISPR Stocks - Bloomington Stock Center
  CRISPR system stocks for site-specific mutagenesis can be obtained at BDSC.
• CRISPR Stocks - Description of CRISPR fly design cas9 stocks
  CRISPR fly design.
• DrosDel Project
  Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at DrosDel.
• Drosophila Gene Search Project
  Information about ordering P{GS} lines can be found at Kyoto Stock Center DGGR.
• DSSC, Drosophila Species Stock Center
  Approximately 270 different Drosophila species at the University of San Diego Drosophila Stock Center can be obtained at DSSC.
• EHIME-Fly, Ehime Drosophila Species Stock Center of Japan
  A collection of wild-type strains of D. melanogaster and strains of over 130 species can be found at EHIME-Fly.
• Exelixis Collection at Harvard Medical School
  Information about ordering the Exelixis stocks at Harvard Medical School can be found at Exelixis Collection.
• FlyORF
  A collection of well-characterized transgenic D. melanogaster UAS-ORF lines produced and distributed by the Zurich ORFeome Project at FlyORF.
• FlyTrap Stock Collection
  Information about GFP protein trap lines from the FlyTrap project at FlyTrap.
• FlyView Stock Collection
  Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at FlyView.
• Gene Disruption Project (GDP)
  Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at GDP and schematic drawings of the transposons can be found on the Transposons page.
• Kyorin-Fly
  A collection of mutant and wildtype stocks of Drosophila species, especially the Drosophila ananassae species subgroup, D. auraria complex, and D. hydei. Kyorin-Fly.
• Kyoto Stock Center, DGGR
  Information about stocks at the Department of Drosophila Genomics and Genetics Resources (DGGR) at the Kyoto Institute of Technology, Japan can be found at Kyoto Stock Center, DGGR.
• MiMIC RMCE Lines
  Information about a collection of protein trap lines containing the EGFP-FlAsH-Strep-TEV-3xFlag tag using the RMCE technique at GDP.
• NIG-FLY: Fly Stocks of the National Institute of Genetics
  Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at NIG-Fly.
• Tagged FlyFos TransgeneOme (fTRG)
  The "Tagged FlyFos TransgeneOme" library is comprised of a total of over 900 transgenic fly lines, covering over 825 different genes. Each line has been engineered to tag a specific protein with a multi-epitope tag at its C-terminus, for use in a variety of downstream applications including live imaging, subcellular localization, and interaction proteomics of selected gene products at all stages of Drosophila development. The transgenic lines are available at Vienna Drosophila Resource Center (VDRC). The collection of ~10,000 GFP-tagged fosmid clones from which transgenic lines were generated is available at Source BioScience.
• Tsinghua Fly Center
  Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at THFC.
• Vienna Drosophila RNAi Center
  The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries at VDRC.

CRISPR Vectors

• CRISPR/Cas Vectors from Simon Bullock Lab
  A set tools for efficient germline and somatic genome engineering in Drosophila melanogaster, available at the addgene plasmid repository, Cambridge, MA, USA

• CRISPR Plasmids - CRISPR fly design cas9 plasmids description
  CRISPR fly design, Simon Bullock, MRC, Cambridge, UK

Expression Vectors

• Drosophila Gateway Vectors
  A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC
• ExpreS2ion Biotechnologies
  ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. The ExpreS2 Platform

Cell Lines and Hybridomas

• Cellosaurus
  A thesaurus of cell lines which attempts to list all cell lines used in biomedical research. Cellosaurus
• Drosophila Genomics Resource Center (DGRC)
  Collects and distributes cell lines. More information is available at DGRC
• Developmental Studies Hybridoma Bank
  The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at DSHB.
• ATCC
  The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at ATCC.

Antibodies

• Antibody Registry
• Clontech
• Cocalico Biologicals
• Developmental Studies Hybridoma Bank
• Millipore
• Novus Biologicals
• Sigma-Aldrich
• US Biological

Transgenic, Targeted Mutation, and Other Services

• Aktogen behaviour genetic services
  Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. Aktogen
• BestGene Transgenic Service
  The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at BestGene.
• C-CAMP Centre for Cellular and Molecular Platforms
  A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at C_CAMP.
• Fly Facility
  Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at Fly Facility.
• GenetiVision Corporation
  GenetiVision offers a wide range of transgenic and molecular biology services, including P-element transgenesis and Phi-C31 mediated site-specific integration, CRISPR design and construct building, RMCE, MiMIC, and more. Please see Genetivision for complete details.
• The Genome Engineering Production Group
  The GEPG at Harvard Medical School currently builds custom CRISPR sgRNA constructs for knock-out, knock-in or other projects. GEPB
• GenScript
  A US based company offering gene construction, mutagenesis and subcloning services, as well as a large array of other products and services. GenScript
• Rainbow Transgenic Flies
  Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at Rainbow Transgenic Flies.
• Union Biometrica
  COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at Union Biometrica.

RNA

Clontech
Clontech of BD Biosciences offers D. melanogaster embryo and adult Poly A RNA at Clontech.

RNAi

• Cell-based RNAi (DRSC)
  DRSC/TRiP Functional Genomics Resources at Harvard Medical School provides reagents and resources for cell-based RNAi screens. DRSC
• Gene Silencing Platform
  The GSP at CIC bioGUNE provides cell-based screens using RNAi. CIC bioGUNE
• Helmholtz RNAi Screening Platform
  The Helmholtz RNAi Screening Platform at the University of Heidelberg provides RNAi screening in and computational tools for Drosophila and human cells. RNAi Screening Wiki.
• NIG-FLY RNAi Resources
  NIG-Fly in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request. NIG-Fly
• NYU RNAi Core
  The NYU RNAi Core Facility at the Langone Medical Center in New York provides high-throughput RNAi screens. Drosophila screens are offered in collaboration with the DRSC. NYU RNAi Core
• Open Biosystems
  Open Biosystems provides the Drosophila RNAi Collection version 1.0, a collection of dsDNA constructs for generation of dsRNA. Open Biosystems
• Sheffield RNAi Screening Facility
  The SRSF at Biomedical Science at the University of Sheffield advertises low-cost RNAi screens in Drosophila cells. Sheffield RNAi Screening Facility
• TRiP, Transgenic RNAi Project
  DRSC/TRiP Functional Genomics Resources at the Harvard Medical School has created >12,000 transgenic Drosophila RNAi stocks and developed efficient vectors for RNAi that work in all tissues. Stocks are distributed through the Bloomington Drosophila Stock Center (BDSC). TRiP
• Tsinghua Fly Center
  Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at THFC.
• VDRC, Vienna Drosophila RNAi Center
  The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries. VDRC

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