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==BAC Genomic clones and Filters== | ==BAC Genomic clones and Filters== | ||
| − | * | + | * One BAC clone library made from DNA of the same <span class="italic">y<sup>1</sup> ; cn<sup>1</sup> bw<sup>1</sup> sp<sup>1</sup></span> stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong. |
| − | * Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P | + | * BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm |
| − | * For information and resources related to the P[acman] recombineering technique see: | + | * Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html |
| + | * For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org | ||
| − | == | + | ==P1 Genomic Clones== |
| − | * | + | * As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the [http://www.fruitfly.org/about/materials/order.p1.html BDGP]. |
| + | * For additional information about the Berkeley Drosophila Genome Project see [http://www.fruitfly.org/ http://www.fruitfly.org]. | ||
| + | |||
| + | ==Cosmid Genomic Clones and Filters== | ||
| + | |||
| + | * EDGP cosmids are available from the Geneservice:<br />http://www.geneservice.co.uk/products/clones/drosophila.jsp. | ||
| + | * Cosmids are available either singly or on high-density filters. | ||
| + | The members of the European <span class="italic">Drosophila</span> Genome mapping consortium are: | ||
| + | :* F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.<br />Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete. | ||
| + | :* D.M. Glover and R.D.C. Saunders,<br />Department of Biochemistry, University of Dundee, Dundee, Scotland. | ||
| + | :* M. Ashburner,<br />Department of Genetics, University of Cambridge, Cambridge, England. | ||
| + | :* J. Modolell,<br />Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,<br />with initial funding from the Fondation Schlumberger. | ||
| + | Supported by the Stimulation Action Programme of the European Community, | ||
| + | with initial funding from the Fondation Schlumberger. | ||
==YAC Genomic clones== | ==YAC Genomic clones== | ||
| − | A complete set of | + | A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him. |
Contact: | Contact: | ||
| Line 19: | Line 33: | ||
==Sibling Species Genomic Libraries== | ==Sibling Species Genomic Libraries== | ||
Fosmid Libraries from BACPAC Resources<br /> | Fosmid Libraries from BACPAC Resources<br /> | ||
| − | : | + | :Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from ''Drosophila erecta'', ''D. pseudoobscura'', ''D. virilis'', and ''D. willistoni''. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm |
=cDNA (EST Clones and Libraries)= | =cDNA (EST Clones and Libraries)= | ||
| − | * '''Addgene'''<br />Addgene helps labs archive and share their plasmids that have appeared in publications.<br />[http://www.addgene.org/ | + | * '''Addgene'''<br />Addgene helps labs archive and share their plasmids that have appeared in publications.<br />[http://www.addgene.org/pgvec1?f=c&cmd=browse http://www.addgene.org/pgvec1?f=c&cmd=browse] |
| − | * '''Berkeley Drosophila Genome Project'''<br /> | + | * '''Berkeley Drosophila Genome Project'''<br />The information is provided by BDGP. If you don't find the information you need here check http://www.fruitfly.org/about/materials/ for possible updates. |
| − | * '''Brian Oliver Laboratory'''<br />Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from | + | * '''Brian Oliver Laboratory'''<br />Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from the Geneservice in the UK. <br />Ordering information can be found at http://www.geneservice.co.uk/products/cdna/dros_cdna.jsp |
| − | * '''cDNA library aliquots'''<br />Aliquots from a number of the BDGP cDNA libraries are available from the labs on this [http://www.fruitfly.org/about/materials/cDNA-list.html ''' | + | * '''cDNA library aliquots'''<br />Aliquots from a number of the BDGP cDNA libraries are available from the labs on this [http://www.fruitfly.org/about/materials/cDNA-list.html list]. |
| − | * '''Drosophila Gene Collection (DGC)'''<br />Contact [http://bacpac.chori.org/ '''BACPAC Resources'''], [http://www. | + | * '''Clontech'''<br />Clontech (BD Biosciences) offers ''D. melanogaster'' embryo and larva 5'-STRETCH cDNA libraries at http://www.clontech.com/products/cat/HTML/1186.shtml |
| − | * '''Drosophila Genomics Resource Center (DGRC)'''<br />Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at | + | * '''Drosophila Gene Collection (DGC)'''<br />Contact [http://bacpac.chori.org/ '''BACPAC Resources'''], [http://www.openbiosystems.com/Genomics/Model Organism Resources/Fly Resources/Drosophila Gene Collection (DG/ '''Open Biosystems'''], [http://www.geneservice.co.uk/products/cdna/DGC.jsp '''Geneservice'''], or the [http://dgrc.cgb.indiana.edu/ '''DGRC''']<br />for clones in [http://www.fruitfly.org/DGC/DGC.txt DGCr1.0] or [http://www.fruitfly.org/DGC/dgc2.txt DGCr2.0]. |
| − | * '''EST Project cDNA Clones'''<br />Contact the [ | + | * '''Drosophila Genomics Resource Center (DGRC)'''<br />Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at http://dgrc.cgb.indiana.edu/ |
| + | * '''EST Project cDNA Clones'''<br />Contact the [http://dgrc.cgb.indiana.edu/ '''DGRC'''] for cDNA clones from the BDGP EST collection. | ||
=Arrays and Primers= | =Arrays and Primers= | ||
| − | * '''Affymetrix'''<br />The Affymetrix GeneChip Drosophila Genome | + | * '''Affymetrix'''<br />The Affymetrix GeneChip Drosophila Genome Array for gene expression analysis based on Release 3.1 annotations can be obtained at http://www.affymetrix.com/estore/esearch/search.jsp?pd=131433&N=4294967292<br />The Affymetrix Drosophila Tiling 2.0R Array based on Genome Release 5 with comprehensive genome coverage at 38bp resolution can be obtained here: http://www.affymetrix.com/products/arrays/specific/drosophila_tiling2_0r.affx |
| − | * '''Drosophila Genomics Resource Center (DGRC)'''<br />Produces and distributes DNA microarray slides. More information is available at | + | * '''Drosophila Genomics Resource Center (DGRC)'''<br />Produces and distributes DNA microarray slides. More information is available at http://dgrc.cgb.indiana.edu/ |
| − | * '''FlyChip'''<br />FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at | + | * '''FlyChip'''<br />FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/ |
| − | * '''International Drosophila Array Consortium (INDAC)'''<br />An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at | + | * '''International Drosophila Array Consortium (INDAC)'''<br />An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at http://www.flychip.org.uk/services/core/FL003/ |
| − | * '''UK Drosophila Affymetrix Microarray Service'''<br />A publicly funded microarray service for the UK Drosophila research community. More information is available at | + | * '''UK Drosophila Affymetrix Microarray Service'''<br />A publicly funded microarray service for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/services/affy/ |
| − | * '''FlyPrimerBank'''<br />An online database for ''Drosophila melanogaster'' gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at [http://www.flyrnai.org/flyprimerbank | + | * '''FlyPrimerBank'''<br />An online database for ''Drosophila melanogaster'' gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at [http://www.flyrnai.org/flyprimerbank www.flyrnai.org/flyprimerbank] |
=Stocks= | =Stocks= | ||
| − | * '''Bloomington Stock Center'''<br />Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at [http://flystocks.bio.indiana.edu/ | + | * '''Bloomington Stock Center'''<br />Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at [http://flystocks.bio.indiana.edu/ http://flystocks.bio.indiana.edu] |
| − | + | * '''DrosDel Project'''<br />Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at http://www.drosdel.org.uk/ | |
| − | + | * '''Drosophila Gene Search Project'''<br />Information about ordering P{GS} lines can be found at http://kyotofly.kit.jp/stocks/documents/GS_lines.html | |
| − | * '''DrosDel Project'''<br />Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at | + | * '''Ehime Drosophila Species Stock Center of Japan'''<br />A collection of wild-type strains of ''D. melanogaster'' and strains of some 30 species can be found at http://kyotofly.kit.ac.jp/ehime/ |
| − | * '''Drosophila Gene Search Project'''<br />Information about ordering P{GS} lines can be found at | + | * '''Exelixis Collection at Harvard Medical School'''<br />Information about ordering the Exelixis stocks at Harvard Medical School can be found at http://drosophila.med.harvard.edu/ |
| − | * ''' | + | * '''FlyORF'''<br />A collection of well-characterized transgenic ''D. melanogaster'' UAS-ORF lines produced and distributed by the Zurich ORFeome Project. http://flyorf.ch/ |
| − | + | * '''FlyTrap Stock Collection'''<br />GFP protein trap lines from the FlyTrap project can be obtained at Yale University, USA at http://flytrap.med.yale.edu/ | |
| − | * '''Exelixis Collection at Harvard Medical School'''<br />Information about ordering the Exelixis stocks at Harvard Medical School can be found at | + | * '''FlyView Stock Collection'''<br />Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at http://flyview.uni-muenster.de/ |
| − | * '''FlyORF'''<br />A collection of well-characterized transgenic ''D. melanogaster'' UAS-ORF lines produced and distributed by the Zurich ORFeome Project | + | * '''Gene Disruption Project (GDP)'''<br />Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at http://flypush.imgen.bcm.tmc.edu/pscreen/ |
| − | * '''FlyTrap Stock Collection'''<br /> | + | * '''Kyorin-Fly'''<br />A collection of mutant and wildtype stocks of Drosophila species, especially the ''Drosophila ananassae'' species subgroup, ''D. auraria'' complex, and ''D. hydei''. http://kyotofly.kit.jp/cgi-bin/kyorin/index.cgi |
| − | * '''FlyView Stock Collection'''<br />Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at | + | * '''Kyoto Stock Center'''<br />Information about stocks at the Drosophila Genetic Resource Center (DGRC, Japan) at the Kyoto Institute of Technology, Japan can be found at http://www.dgrc.kit.ac.jp/en/index.html |
| − | * '''Gene Disruption Project (GDP)'''<br />Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at | + | * '''MiMIC RMCE Lines'''<br />A collection of protein trap lines containing the EGFP-FlAsH-Strep-TEV-3xFlag tag using the RMCE technique.<br />http://flypush.imgen.bcm.tmc.edu/pscreen/rmce/ |
| − | * '''Kyorin-Fly'''<br />A collection of mutant and wildtype stocks of Drosophila species, especially the ''Drosophila ananassae'' species subgroup, ''D. auraria'' complex, and ''D. hydei''. | + | * '''NIG-FLY: Fly Stocks of the National Institute of Genetics'''<br />Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at http://www.shigen.nig.ac.jp/fly/nigfly/ |
| − | * '''Kyoto Stock Center | + | * '''Tuebingen Drosophila Stock Collection'''<br />A [http://www.eb.tuebingen.mpg.de/departments/3-genetics/drosophila/drosophila-stock-collection/drosophila-stock-collection?set_language=en&cl=en list of approximately 2,000 Drosophila stocks] at the Max-Planck-Institute for Developmental Biology. |
| − | * '''MiMIC RMCE Lines'''<br /> | + | * '''Tsinghua Fly Center'''<br />Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at http://center.biomed.tsinghua.edu.cn/public/eq-category/modelanimalfacility/ |
| − | * '''NIG-FLY: Fly Stocks of the National Institute of Genetics'''<br />Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at | + | * '''UC San Diego Drosophila Stock Center'''<br />Approximately 270 different Drosophila species at University of San Diego, USA can be obtained at https://stockcenter.ucsd.edu/info/welcome.php |
| − | * ''' | + | * '''University of Cambridge FlyProt project'''<br /> Annotations, expression patterns and sequence data for the YFP protein trap lines from the FlyProt project can be viewed on the Flannotator at http://www.flyprot.org. Stocks are available from the [http://kyotofly.kit.jp/cgi-bin/stocks/index.cgi Kyoto Stock Center.] |
| − | * '''Tsinghua Fly Center'''<br />Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at | + | * '''Vienna Drosophila RNAi Center'''<br /> The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic <span class="italic">Drosophila''' RNAi libraries, at http://stockcenter.vdrc.at/control/main |
| − | * ''' | ||
| − | |||
| − | |||
| − | * ''' | ||
| − | |||
| − | * ''' | ||
=Expression Vectors= | =Expression Vectors= | ||
| − | * '''Drosophila Gateway Vectors'''<br />A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the [ | + | * '''Drosophila Gateway Vectors'''<br />A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the [http://dgrc.cgb.indiana.edu/ DGRC] |
| − | * '''ExpreS2ion Biotechnologies'''<br />ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. [http://www. | + | * '''ExpreS2ion Biotechnologies'''<br />ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. [http://www.ExpreS2ionBio.com/ ExpreS2ion Biotechnologies] |
=Cell Lines and Hybridomas= | =Cell Lines and Hybridomas= | ||
| − | + | * '''Drosophila Genomics Resource Center (DGRC)'''<br />Collects and distributes cell lines. More information is available at http://dgrc.cgb.indiana.edu/ | |
| − | * '''Drosophila Genomics Resource Center (DGRC)'''<br />Collects and distributes cell lines. More information is available at | + | * '''Developmental Studies Hybridoma Bank'''<br />The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at http://dshb.biology.uiowa.edu |
| − | * '''Developmental Studies Hybridoma Bank'''<br />The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at | + | * '''ATCC'''<br />The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at http://www.atcc.org/ |
| − | * '''ATCC'''<br />The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at | ||
=Antibodies= | =Antibodies= | ||
| Line 85: | Line 93: | ||
=Transgenic, Targeted Mutation, and Other Services= | =Transgenic, Targeted Mutation, and Other Services= | ||
| − | * '''Aktogen behaviour genetic services'''<br />Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. | + | * '''Aktogen behaviour genetic services'''<br />Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. http://www.aktogen.com/ |
| − | * '''BestGene Transgenic Service'''<br />The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at | + | * '''BestGene Transgenic Service'''<br />The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at http://www.thebestgene.com/ |
| − | * '''C-CAMP Centre for Cellular and Molecular Platforms'''<br />A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at | + | * '''C-CAMP Centre for Cellular and Molecular Platforms'''<br />A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at http://ccamp.res.in/Fly-facility |
| − | * '''Drosophila TILLING Project'''<br />The goal of Fly-TILL is to rapidly deliver to the Drosophila community EMS-induced mutations in requested genes via high-throughput reverse genetic screens. More information is available at | + | * '''Drosophila TILLING Project'''<br />The goal of Fly-TILL is to rapidly deliver to the Drosophila community EMS-induced mutations in requested genes via high-throughput reverse genetic screens. More information is available at http://tilling.fhcrc.org/fly/Welcome_to_Fly-TILL.html |
| − | * '''Fly Facility'''<br />Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at | + | * '''Fly Facility'''<br />Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at http://www.fly-facility.com/ |
| − | * '''GenetiCorps Custom Injection Services'''<br />GenetiCorps offers high quality fly injection services to the Drosophila research community. For more information, please visit | + | * '''Genetic Services'''<br />Genetic Services provides the Drosophila community with services including transgenic production, injection work, genetic screening, stock creation, construct production and consulting. More information is available at [http://www.geneticservices.com/ http://www.geneticservices.com] |
| − | * '''Genetivision, Inc.'''<br />Genetivision offers a transgenic fly service for both delta 2-3 transposase-mediated P-element transgenesis and Phi-C31 mediated site-specific integration. More information is available at | + | * '''GenetiCorps Custom Injection Services'''<br />GenetiCorps offers high quality fly injection services to the Drosophila research community. For more information, please visit http://www.geneticorps.com/ |
| − | * '''The Genome Engineering Production Group'''<br /> The GEPG at Harvard Medical School currently builds custom CRISPR sgRNA constructs for knock-out, knock-in or other projects. | + | * '''Genetivision, Inc.'''<br />Genetivision offers a transgenic fly service for both delta 2-3 transposase-mediated P-element transgenesis and Phi-C31 mediated site-specific integration. More information is available at http://www.genetivision.com/ |
| − | * '''GenScript'''<br /> A US based company offering gene construction, mutagenesis and subcloning services, as well as a large array of other products and services. | + | * '''The Genome Engineering Production Group'''<br /> The GEPG at Harvard Medical School currently builds custom CRISPR sgRNA constructs for knock-out, knock-in or other projects. http://gepg.hms.harvard.edu/ |
| − | * '''Rainbow Transgenic Flies'''<br />Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at | + | * '''GenScript'''<br /> A US based company offering gene construction, mutagenesis and subcloning services, as well as a large array of other products and services. http://www.genscript.com/ |
| − | * '''Union Biometrica'''<br />COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at | + | * '''Rainbow Transgenic Flies'''<br />Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at http://www.rainbowgene.com/ |
| + | * '''Transgenic Drosophila Fly Core'''<br />The Cutaneous Biology Research Center (CRBC) Fly Core at Massachusetts General Hospital/Harvard Medical School provides microinjection services to create transgenic Drosophila fly strains. More information is available at [http://www.flycore.com/ http://www.flycore.com] | ||
| + | * '''Union Biometrica'''<br />COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at http://www.unionbio.com/ | ||
| + | * '''VANEDIS Drosophila injection service'''<br />The VANEDIS Drosophila injection service injects embryos with your construct of interest. More information is available at http://www.vanedis.no | ||
=RNA= | =RNA= | ||
| − | '''Clontech'''<br />Clontech of BD Biosciences offers | + | '''Clontech'''<br />Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at [http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2 http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2] |
=RNAi= | =RNAi= | ||
| − | * '''Drosophila RNAi Screening Center'''<br /> The Drosophila RNAi Screening Center at Harvard Medical School provides high throughput RNAi screens. | + | * '''Drosophila RNAi Screening Center'''<br /> The Drosophila RNAi Screening Center at Harvard Medical School provides high throughput RNAi screens. http://flyrnai.org/RNAi_index.html |
| − | * '''Gene Silencing Platform'''<br /> The GSP at CIC bioGUNE provides cell-based screens using RNAi. | + | * '''Gene Silencing Platform'''<br /> The GSP at CIC bioGUNE provides cell-based screens using RNAi. http://cicbiogune.es/secciones/investigacion/plataformas_tecnologicas.php?idioma=en |
| − | * '''Helmholtz RNAi Screening Platform'''<br /> The Helmholtz RNAi Screening Platform at the University of Heidelberg provides RNAi screening in and computational tools for <span class="italic">Drosophila and human cells. | + | * '''Geneservice'''<br /> Geneservice, in collaboration with Cyclacel Limited, offers a collection of 13,600 RNAi constructs in its Drosophila genome RNAi library. http://www.geneservice.co.uk/products/rnai/Dros_RNAi.jsp |
| − | * '''NIG-FLY RNAi Resources'''<br /> NIG-Fly in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request. [http://www.shigen.nig.ac.jp/fly/nigfly/index.jsp | + | * '''Helmholtz RNAi Screening Platform'''<br /> The Helmholtz RNAi Screening Platform at the University of Heidelberg provides RNAi screening in and computational tools for <span class="italic">Drosophila''' and human cells. http://b110-wiki.dkfz.de/signaling/wiki/display/rnaiwiki. |
| − | * '''NYU RNAi Core'''<br /> The NYU RNAi Core Facility at the Langone Medical Center in New York provides high-throughput RNAi screens. <span class="italic">Drosophila screens are offered in collaboration with the DRSC. | + | * '''NIG-FLY RNAi Resources'''<br /> NIG-Fly in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request. [http://www.shigen.nig.ac.jp/fly/nigfly/index.jsp http://www.shigen.nig.ac.jp/fly/nigfly/0] |
| − | * '''Open Biosystems'''<br /> Open Biosystems provides the Drosophila RNAi Collection version 1.0, a collection of dsDNA constructs for generation of dsRNA. | + | * '''NYU RNAi Core'''<br /> The NYU RNAi Core Facility at the Langone Medical Center in New York provides high-throughput RNAi screens. <span class="italic">Drosophila''' screens are offered in collaboration with the DRSC. http://rnainy.med.nyu.edu/ |
| − | * '''Sheffield RNAi Screening Facility'''<br /> The SRSF at Biomedical Science at the University of Sheffield advertises low-cost RNAi screens in <span class="italic">Drosophila cells. | + | * '''Open Biosystems'''<br /> Open Biosystems provides the Drosophila RNAi Collection version 1.0, a collection of dsDNA constructs for generation of dsRNA. http://www.openbiosystems.com/RNAi/DrosophilaRNAicollections/ |
| − | * ''' | + | * '''Sheffield RNAi Screening Facility'''<br /> The SRSF at Biomedical Science at the University of Sheffield advertises low-cost RNAi screens in <span class="italic">Drosophila''' cells. http://www.rnai.group.shef.ac.uk/ |
| − | * '''Tsinghua Fly Center'''<br />Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at | + | * '''Transgenic RNAi Project'''<br /> TRiP at the Harvard Medical School plans to generate 6,250 transgenic RNAi lines targeted to attP2 on chromosome 3. Stocks are distributed through BDSC. http://www.flyrnai.org/TRiP-HOME.html |
| − | * ''' | + | * '''Tsinghua Fly Center'''<br />Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at http://center.biomed.tsinghua.edu.cn/public/eq-category/modelanimalfacility/ |
| + | * '''Vienna Drosophila RNAi Center'''<br /> The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic <span class="italic">Drosophila''' RNAi libraries. http://www.vdrc.at | ||
=Suggest a tool or resource= | =Suggest a tool or resource= | ||
Revision as of 17:36, 21 October 2015
Genomic Clones
BAC Genomic clones and Filters
- One BAC clone library made from DNA of the same y1 ; cn1 bw1 sp1 stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
- BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
- Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
- For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org
P1 Genomic Clones
- As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
- For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.
Cosmid Genomic Clones and Filters
- EDGP cosmids are available from the Geneservice:
http://www.geneservice.co.uk/products/clones/drosophila.jsp. - Cosmids are available either singly or on high-density filters.
The members of the European Drosophila Genome mapping consortium are:
- F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete. - D.M. Glover and R.D.C. Saunders,
Department of Biochemistry, University of Dundee, Dundee, Scotland. - M. Ashburner,
Department of Genetics, University of Cambridge, Cambridge, England. - J. Modolell,
Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
with initial funding from the Fondation Schlumberger.
- F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.
YAC Genomic clones
A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.
Contact:
Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.
Sibling Species Genomic Libraries
Fosmid Libraries from BACPAC Resources
- Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm
cDNA (EST Clones and Libraries)
- Addgene
Addgene helps labs archive and share their plasmids that have appeared in publications.
http://www.addgene.org/pgvec1?f=c&cmd=browse - Berkeley Drosophila Genome Project
The information is provided by BDGP. If you don't find the information you need here check http://www.fruitfly.org/about/materials/ for possible updates. - Brian Oliver Laboratory
Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from the Geneservice in the UK.
Ordering information can be found at http://www.geneservice.co.uk/products/cdna/dros_cdna.jsp - cDNA library aliquots
Aliquots from a number of the BDGP cDNA libraries are available from the labs on this list. - Clontech
Clontech (BD Biosciences) offers D. melanogaster embryo and larva 5'-STRETCH cDNA libraries at http://www.clontech.com/products/cat/HTML/1186.shtml - Drosophila Gene Collection (DGC)
Contact BACPAC Resources, Organism Resources/Fly Resources/Drosophila Gene Collection (DG/ Open Biosystems, Geneservice, or the DGRC
for clones in DGCr1.0 or DGCr2.0. - Drosophila Genomics Resource Center (DGRC)
Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at http://dgrc.cgb.indiana.edu/ - EST Project cDNA Clones
Contact the DGRC for cDNA clones from the BDGP EST collection.
Arrays and Primers
- Affymetrix
The Affymetrix GeneChip Drosophila Genome Array for gene expression analysis based on Release 3.1 annotations can be obtained at http://www.affymetrix.com/estore/esearch/search.jsp?pd=131433&N=4294967292
The Affymetrix Drosophila Tiling 2.0R Array based on Genome Release 5 with comprehensive genome coverage at 38bp resolution can be obtained here: http://www.affymetrix.com/products/arrays/specific/drosophila_tiling2_0r.affx - Drosophila Genomics Resource Center (DGRC)
Produces and distributes DNA microarray slides. More information is available at http://dgrc.cgb.indiana.edu/ - FlyChip
FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/ - International Drosophila Array Consortium (INDAC)
An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at http://www.flychip.org.uk/services/core/FL003/ - UK Drosophila Affymetrix Microarray Service
A publicly funded microarray service for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/services/affy/ - FlyPrimerBank
An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at www.flyrnai.org/flyprimerbank
Stocks
- Bloomington Stock Center
Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at http://flystocks.bio.indiana.edu - DrosDel Project
Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at http://www.drosdel.org.uk/ - Drosophila Gene Search Project
Information about ordering P{GS} lines can be found at http://kyotofly.kit.jp/stocks/documents/GS_lines.html - Ehime Drosophila Species Stock Center of Japan
A collection of wild-type strains of D. melanogaster and strains of some 30 species can be found at http://kyotofly.kit.ac.jp/ehime/ - Exelixis Collection at Harvard Medical School
Information about ordering the Exelixis stocks at Harvard Medical School can be found at http://drosophila.med.harvard.edu/ - FlyORF
A collection of well-characterized transgenic D. melanogaster UAS-ORF lines produced and distributed by the Zurich ORFeome Project. http://flyorf.ch/ - FlyTrap Stock Collection
GFP protein trap lines from the FlyTrap project can be obtained at Yale University, USA at http://flytrap.med.yale.edu/ - FlyView Stock Collection
Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at http://flyview.uni-muenster.de/ - Gene Disruption Project (GDP)
Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at http://flypush.imgen.bcm.tmc.edu/pscreen/ - Kyorin-Fly
A collection of mutant and wildtype stocks of Drosophila species, especially the Drosophila ananassae species subgroup, D. auraria complex, and D. hydei. http://kyotofly.kit.jp/cgi-bin/kyorin/index.cgi - Kyoto Stock Center
Information about stocks at the Drosophila Genetic Resource Center (DGRC, Japan) at the Kyoto Institute of Technology, Japan can be found at http://www.dgrc.kit.ac.jp/en/index.html - MiMIC RMCE Lines
A collection of protein trap lines containing the EGFP-FlAsH-Strep-TEV-3xFlag tag using the RMCE technique.
http://flypush.imgen.bcm.tmc.edu/pscreen/rmce/ - NIG-FLY: Fly Stocks of the National Institute of Genetics
Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at http://www.shigen.nig.ac.jp/fly/nigfly/ - Tuebingen Drosophila Stock Collection
A list of approximately 2,000 Drosophila stocks at the Max-Planck-Institute for Developmental Biology. - Tsinghua Fly Center
Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at http://center.biomed.tsinghua.edu.cn/public/eq-category/modelanimalfacility/ - UC San Diego Drosophila Stock Center
Approximately 270 different Drosophila species at University of San Diego, USA can be obtained at https://stockcenter.ucsd.edu/info/welcome.php - University of Cambridge FlyProt project
Annotations, expression patterns and sequence data for the YFP protein trap lines from the FlyProt project can be viewed on the Flannotator at http://www.flyprot.org. Stocks are available from the Kyoto Stock Center. - Vienna Drosophila RNAi Center
The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries, at http://stockcenter.vdrc.at/control/main
Expression Vectors
- Drosophila Gateway Vectors
A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC - ExpreS2ion Biotechnologies
ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. ExpreS2ion Biotechnologies
Cell Lines and Hybridomas
- Drosophila Genomics Resource Center (DGRC)
Collects and distributes cell lines. More information is available at http://dgrc.cgb.indiana.edu/ - Developmental Studies Hybridoma Bank
The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at http://dshb.biology.uiowa.edu - ATCC
The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at http://www.atcc.org/
Antibodies
- Antibody Registry
- Clontech
- Cocalico Biologicals
- Developmental Studies Hybridoma Bank
- Millipore
- Novus Biologicals
- Sigma-Aldrich
- US Biological
Transgenic, Targeted Mutation, and Other Services
- Aktogen behaviour genetic services
Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. http://www.aktogen.com/ - BestGene Transgenic Service
The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at http://www.thebestgene.com/ - C-CAMP Centre for Cellular and Molecular Platforms
A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at http://ccamp.res.in/Fly-facility - Drosophila TILLING Project
The goal of Fly-TILL is to rapidly deliver to the Drosophila community EMS-induced mutations in requested genes via high-throughput reverse genetic screens. More information is available at http://tilling.fhcrc.org/fly/Welcome_to_Fly-TILL.html - Fly Facility
Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at http://www.fly-facility.com/ - Genetic Services
Genetic Services provides the Drosophila community with services including transgenic production, injection work, genetic screening, stock creation, construct production and consulting. More information is available at http://www.geneticservices.com - GenetiCorps Custom Injection Services
GenetiCorps offers high quality fly injection services to the Drosophila research community. For more information, please visit http://www.geneticorps.com/ - Genetivision, Inc.
Genetivision offers a transgenic fly service for both delta 2-3 transposase-mediated P-element transgenesis and Phi-C31 mediated site-specific integration. More information is available at http://www.genetivision.com/ - The Genome Engineering Production Group
The GEPG at Harvard Medical School currently builds custom CRISPR sgRNA constructs for knock-out, knock-in or other projects. http://gepg.hms.harvard.edu/ - GenScript
A US based company offering gene construction, mutagenesis and subcloning services, as well as a large array of other products and services. http://www.genscript.com/ - Rainbow Transgenic Flies
Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at http://www.rainbowgene.com/ - Transgenic Drosophila Fly Core
The Cutaneous Biology Research Center (CRBC) Fly Core at Massachusetts General Hospital/Harvard Medical School provides microinjection services to create transgenic Drosophila fly strains. More information is available at http://www.flycore.com - Union Biometrica
COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at http://www.unionbio.com/ - VANEDIS Drosophila injection service
The VANEDIS Drosophila injection service injects embryos with your construct of interest. More information is available at http://www.vanedis.no
RNA
Clontech
Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2
RNAi
- Drosophila RNAi Screening Center
The Drosophila RNAi Screening Center at Harvard Medical School provides high throughput RNAi screens. http://flyrnai.org/RNAi_index.html - Gene Silencing Platform
The GSP at CIC bioGUNE provides cell-based screens using RNAi. http://cicbiogune.es/secciones/investigacion/plataformas_tecnologicas.php?idioma=en - Geneservice
Geneservice, in collaboration with Cyclacel Limited, offers a collection of 13,600 RNAi constructs in its Drosophila genome RNAi library. http://www.geneservice.co.uk/products/rnai/Dros_RNAi.jsp - Helmholtz RNAi Screening Platform
The Helmholtz RNAi Screening Platform at the University of Heidelberg provides RNAi screening in and computational tools for Drosophila and human cells. http://b110-wiki.dkfz.de/signaling/wiki/display/rnaiwiki. - NIG-FLY RNAi Resources
NIG-Fly in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request. http://www.shigen.nig.ac.jp/fly/nigfly/0 - NYU RNAi Core
The NYU RNAi Core Facility at the Langone Medical Center in New York provides high-throughput RNAi screens. Drosophila screens are offered in collaboration with the DRSC. http://rnainy.med.nyu.edu/ - Open Biosystems
Open Biosystems provides the Drosophila RNAi Collection version 1.0, a collection of dsDNA constructs for generation of dsRNA. http://www.openbiosystems.com/RNAi/DrosophilaRNAicollections/ - Sheffield RNAi Screening Facility
The SRSF at Biomedical Science at the University of Sheffield advertises low-cost RNAi screens in Drosophila cells. http://www.rnai.group.shef.ac.uk/ - Transgenic RNAi Project
TRiP at the Harvard Medical School plans to generate 6,250 transgenic RNAi lines targeted to attP2 on chromosome 3. Stocks are distributed through BDSC. http://www.flyrnai.org/TRiP-HOME.html - Tsinghua Fly Center
Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at http://center.biomed.tsinghua.edu.cn/public/eq-category/modelanimalfacility/ - Vienna Drosophila RNAi Center
The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries. http://www.vdrc.at
Suggest a tool or resource
- Please contact Flybase to suggest a tool or resource for the list.