Difference between revisions of "FlyBase:Drosophila Online Resources"
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=Atlases, Images and Videos= | =Atlases, Images and Videos= | ||
− | * <b>Atlas of Drosophila Development</b>, An Atlas following the main events of embryogenesis and post-embryonic development:<br />[ | + | * <b>Atlas of Drosophila Development</b>, An Atlas following the main events of embryogenesis and post-embryonic development:<br />[https://www.sdbonline.org/sites/fly/atlas/00atlas.htm Volker Hartenstein and Interactive Fly, Bethesda, MD, USA] |
− | |||
* <b>FlyBase Maps</b>, A collection of illustrated and electron micrograph maps of polytene chromosomes for the 12 originally sequenced Drosophila species:<br />[http://flybase.org/wiki/FlyBase:Maps FlyBase Maps] | * <b>FlyBase Maps</b>, A collection of illustrated and electron micrograph maps of polytene chromosomes for the 12 originally sequenced Drosophila species:<br />[http://flybase.org/wiki/FlyBase:Maps FlyBase Maps] | ||
− | |||
* <b>FlyMove</b>, an Image, Movie and Interactive Shockwave Resource:<br />[http://flymove.uni-muenster.de/ FlyMove, Muenster, Germany] | * <b>FlyMove</b>, an Image, Movie and Interactive Shockwave Resource:<br />[http://flymove.uni-muenster.de/ FlyMove, Muenster, Germany] | ||
* <b>FlyPNS</b>, ''D. melanogaster'' embryonic and larval peripheral nervous system:<br />[http://www.normalesup.org/~vorgogoz/FlyPNS/page1.html FlyPNS, UMPC, Paris, France and Columbia University, NY, USA] | * <b>FlyPNS</b>, ''D. melanogaster'' embryonic and larval peripheral nervous system:<br />[http://www.normalesup.org/~vorgogoz/FlyPNS/page1.html FlyPNS, UMPC, Paris, France and Columbia University, NY, USA] | ||
* <b>FlyView</b>, a Drosophila Image Database:<br />[http://flyview.uni-muenster.de/ FlyView, Muenster, Germany] | * <b>FlyView</b>, a Drosophila Image Database:<br />[http://flyview.uni-muenster.de/ FlyView, Muenster, Germany] | ||
* <b>GETDB</b>, a Gal4 enhancer trap database:<br />[https://kyotofly.kit.jp/stocks/GETDB/getdb.html GETDB, Kyoto Institute of Technology, Kyoto, Japan] | * <b>GETDB</b>, a Gal4 enhancer trap database:<br />[https://kyotofly.kit.jp/stocks/GETDB/getdb.html GETDB, Kyoto Institute of Technology, Kyoto, Japan] | ||
− | * <b> | + | * <b>Jove</b>, videos of experimental techniques:<br />[https://www.jove.com/search? Jove, Journal of Visualized Experiments, USA] |
− | * <b> | + | * <b>MidgutAtlas</b>, Gene expression in five pH-defined regions of the larval midgut of D. melanogaster:<br />[https://flyatlas.gla.ac.uk/MidgutAtlas/index.html Dow laboratory, University of Glascow, Scotland, UK] |
− | * <b>PeptideAtlas</b>, a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments: <br />[ | + | * <b>PeptideAtlas</b>, a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments: <br />[https://peptideatlas.org/ Seattle Proteome Center, Seattle, Washington, USA] |
* <b>Virtual Fly Brain</b> - A 3D brain browser with point and click searches for neurons, transgenes and phenotypes:<br />[http://www.virtualflybrain.org Virtual Fly Brain, Edinburgh and Cambridge, UK] | * <b>Virtual Fly Brain</b> - A 3D brain browser with point and click searches for neurons, transgenes and phenotypes:<br />[http://www.virtualflybrain.org Virtual Fly Brain, Edinburgh and Cambridge, UK] | ||
+ | |||
+ | =Cell Lines and Cell Culture= | ||
+ | * <b>Cellosaurus</b>: A thesaurus of cell lines which attempts to list all cell lines used in biomedical research.<br /> [https://www.cellosaurus.org/ Swiss Institute of Bioinformatics, Geneva, Switzerland] | ||
+ | * <b>DGRC: Cell Line Catalog</b>: <br /> [https://dgrc.bio.indiana.edu/ Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC: Cell Culture Protocols</b>: <br />[https://dgrc.bio.indiana.edu/Protocols?tab=cells Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC: Cell Culture FAQ Page</b>: <br />[https://dgrc.bio.indiana.edu/FAQs?tab=cells Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC: Feeder Cells</b>: <br />[https://dgrc.bio.indiana.edu/cells/FeederCells Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC: Fly Extract</b>:<br />[https://dgrc.bio.indiana.edu/cells/FlyExtract Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DRSC: General information about cell lines</b>: <br />[https://fgr.hms.harvard.edu/drosophila-cultured-cell-lines DRSC, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>DSRC: Resource information about CRISPR modified cell lines</b>: <br />[https://fgr.hms.harvard.edu/crispr-modified-cell-lines DRSC, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>DRSC: Protocol information about CRISPR modification of cell lines</b>: <br />[https://fgr.hms.harvard.edu/cell-based-crispr DRSC, Harvard Medical School, Boston, MA, USA] | ||
=CRISPRs and TALENs= | =CRISPRs and TALENs= | ||
− | * <b>CCTop</b> CRISPR/Cas9 optimum target finder:<br />[ | + | * <b>Addgene sgRNA Libraries</b>: Pooled sgRNA libraries for pooled CRISPR cell screening <br />[https://www.addgene.org/search/catalog/pooled-libraries/?q=Drosophila Addgene, Watertown, MA, USA] |
+ | * <b>CCTop</b> CRISPR/Cas9 optimum target finder:<br />[https://cctop.cos.uni-heidelberg.de/index.html Center for Organismal Studies, Heidelberg University, Heidelberg, DE] | ||
* <b>CHOPCHOP</b> CRISPR/Cas9 target online predictor, mammalian and other major model organisms including fly:<br />[https://chopchop.cbu.uib.no/ University of Bergen, Bergen, Norway] | * <b>CHOPCHOP</b> CRISPR/Cas9 target online predictor, mammalian and other major model organisms including fly:<br />[https://chopchop.cbu.uib.no/ University of Bergen, Bergen, Norway] | ||
* <b>CRISPR-ERA</b> A Fast and Comprehensive Guide RNA Design Tool for Genome Editing, Repression and Activation:<br />[http://crispr-era.stanford.edu// Lei Stanley Qi Lab and Xiaowo Wang Lab, Stanford University, Palo Alto, CA, USA] | * <b>CRISPR-ERA</b> A Fast and Comprehensive Guide RNA Design Tool for Genome Editing, Repression and Activation:<br />[http://crispr-era.stanford.edu// Lei Stanley Qi Lab and Xiaowo Wang Lab, Stanford University, Palo Alto, CA, USA] | ||
− | * <b>CRISPR fly design</b> -- reagents, protocols, and results from fly CRISPR/Cas experiments:<br />[http:// | + | * <b>CRISPR fly design</b> -- reagents, protocols, and results from fly CRISPR/Cas experiments:<br />[http://crisprflydesign.org/ Bullolk Lab, MRC Laboratory of Molecular Biology, Cambridge, UK] |
− | + | * <b>CRISPResso2 </b> CRISPR Design Tool:<br />[http://crispresso.pinellolab.org/submission Broad Institute, Cambridge, MA, Harvard Medical School, Boston, MA, Massachusetts General Hospital, Boston, MA, Dana Farber Cancer Center, Boston, MA, Children's Hospital, Boston, MA] | |
− | * <b>CRISPResso2 </b> CRISPR Design Tool:<br />[ | ||
* <b>CRISPRscan</b> Novel scoring algorithm for selecting sgRNAs<br />[http://www.crisprscan.org// Giraldez Lab, Yale University, New Haven, CT, USA] | * <b>CRISPRscan</b> Novel scoring algorithm for selecting sgRNAs<br />[http://www.crisprscan.org// Giraldez Lab, Yale University, New Haven, CT, USA] | ||
− | * <b>DRSC CRISPR Efficiency Tool for Assessment of Designs</b>:<br />[ | + | * <b>DGRC Cell lines and vector reagents for CRISPR</b>:<br />[https://dgrc.bio.indiana.edu/Search?category=&query=crispr Drosophila Genomics Resource Center (DGRC), Indiana University, Bloomington, IN, USA] |
− | * <b>DRSC Find CRISPRs</b>, Drosophila CRISPR gRNA design search tool:<br />[ | + | * <b>DGRC sgRNA libraries</b>:<br />[https://dgrc.bio.indiana.edu/gRNA Drosophila Genomics Resource Center (DGRC), Indiana University, Bloomington, IN, USA] |
+ | * <b>DRSC CRISPR Efficiency Tool for Assessment of Designs</b>:<br />[https://www.flyrnai.org/evaluateCrispr/ DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>DRSC Find CRISPRs</b>, Drosophila CRISPR gRNA design search tool:<br />[https://www.flyrnai.org/crispr2/ DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | ||
* <b>TRiP CRISPR fly stock information</b>:<br />[https://fgr.hms.harvard.edu/crispr-fly-stocks-and-vectors DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | * <b>TRiP CRISPR fly stock information</b>:<br />[https://fgr.hms.harvard.edu/crispr-fly-stocks-and-vectors DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | ||
* <b>DRSC cell-based CRISPR modification</b>:<br />[https://fgr.hms.harvard.edu/publications/technology/fly-cell-based-crispr DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | * <b>DRSC cell-based CRISPR modification</b>:<br />[https://fgr.hms.harvard.edu/publications/technology/fly-cell-based-crispr DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | ||
* <b>E-CRISP</b>, Design of CRISPR Constructs:<br />[http://www.e-crisp.org/E-CRISP/designcrispr.html DKFZ/Boutros lab, Heildelberg, Germany] | * <b>E-CRISP</b>, Design of CRISPR Constructs:<br />[http://www.e-crisp.org/E-CRISP/designcrispr.html DKFZ/Boutros lab, Heildelberg, Germany] | ||
* <b>E-TALEN</b>, Design of TALEN Constructs:<br />[http://www.e-talen.org/E-TALEN/ DKFZ/Boutros lab, Heildelberg, Germany] | * <b>E-TALEN</b>, Design of TALEN Constructs:<br />[http://www.e-talen.org/E-TALEN/ DKFZ/Boutros lab, Heildelberg, Germany] | ||
− | * <b>flyCRISPR</b>, | + | * <b>flyCRISPR</b>, Overview: <br />[https://flycrispr.org/ O'Connor-Giles, Wildonger, and Harrison Labs, University of Wisconsin-Madison, WI, USA] |
− | * <b>flyCRISPR</b>, | + | * <b>flyCRISPR</b>, Target Finder:<br />[https://flycrispr.org/target-finder/ O'Connor-Giles Lab, University of Wisconsin-Madison, WI, USA] |
− | * <b>Genome Engineering by CRISPR/Cas9 in Drosophila</b>:<br />[ | + | * <b>Genome Engineering by CRISPR/Cas9 in Drosophila</b>:<br />[https://shigen.nig.ac.jp/fly/nigfly/cas9/ NIG/FLY/Ueda lab, Mishima, Japan] |
* <b>Mojo Hand</b>, A TALEN Design Tool:<br />[http://www.talendesign.org/ Ekker lab, Mayo Clinic, Rochester, MN, USA] | * <b>Mojo Hand</b>, A TALEN Design Tool:<br />[http://www.talendesign.org/ Ekker lab, Mayo Clinic, Rochester, MN, USA] | ||
− | |||
− | |||
* <b>TALengineering.org</b>, A Comprehensive Resource for Engineered TAL Effector Technology:<br />[http://talengineering.org/ Ekker lab, Joung Lab, MGH, Boston, USA] | * <b>TALengineering.org</b>, A Comprehensive Resource for Engineered TAL Effector Technology:<br />[http://talengineering.org/ Ekker lab, Joung Lab, MGH, Boston, USA] | ||
+ | * <b>TRiP: Overview of sgRNA vectors</b>:<br />[https://fgr.hms.harvard.edu/sgrna-vectors TRiP, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>Zhang Lab CRISPR Plasmids Available from Addgene</b> CRISPR Plasmids:<br />[https://www.addgene.org/crispr/zhang/ Zhang Lab, MIT, Boston, MA, USA] | ||
=Data Repositories= | =Data Repositories= | ||
− | * <b>Array Express</b> - Functional Genomics Data:<br />[ | + | * <b>Array Express</b> - Functional Genomics Data:<br />[https://www.ebi.ac.uk/arrayexpress/ Array Express, EMBL-EBI, Hinxton, UK] |
− | * <b>Berkeley Drosophila Genome Project (BDGP)</b>:<br />[ | + | * <b>Berkeley Drosophila Genome Project (BDGP)</b>:<br />[https://www.fruitfly.org/ BDGP, University of California, Berkeley, USA] |
− | * <b>DDBJ</b>, the DNA Data Bank of Japan:<br />[ | + | * <b>DDBJ</b>, the DNA Data Bank of Japan:<br />[https://www.ddbj.nig.ac.jp/index-e.html DDBJ, National Institute of Genetics, Mishima, Japan] |
− | * <b>EMBL-EBI</b>, The European Bioinformatics Institute:<br />[ | + | * <b>EMBL-EBI</b>, The European Bioinformatics Institute:<br />[https://www.ebi.ac.uk/ EMBL-EBI, Hinxton, UK] |
− | * <b>ENA</b>, European Nucleotide Archive:<br />[ | + | * <b>ENA</b>, European Nucleotide Archive:<br />[https://www.ebi.ac.uk/ena/browser/home ENA, European Nucleotide Archive, EMBL-EBI, Hinxton, UK] |
* <b>ENCODE</b>, Encyclopedia of DNA Elements (including modENCODE and modERN):<br />[https://www.encodeproject.org/ ENCODE, Stanford University, Stanford, USA] | * <b>ENCODE</b>, Encyclopedia of DNA Elements (including modENCODE and modERN):<br />[https://www.encodeproject.org/ ENCODE, Stanford University, Stanford, USA] | ||
− | + | * <b>Gene Disruption Project (GDP)</b>:<br />[https://flypush.research.bcm.edu/pscreen/index.php GDP, Baylor College of Medicine, Texas]<br />[https://flypush.research.bcm.edu/pscreen/transposons.html Schematic drawings of Transposons] | |
− | * <b>Gene Disruption Project (GDP)</b>:<br />[ | + | * <b>GenBank</b>,the NIH genetic sequence database:<br />[https://www.ncbi.nlm.nih.gov/genbank/ GenBank, NCBI, Bethesda, USA] |
− | * <b>GenBank</b>,the NIH genetic sequence database:<br />[ | + | * <b>GEO Datasets</b>, Gene Expression Omnibus:<br />[https://www.ncbi.nlm.nih.gov/geo/info/datasets.html NCBI, Bethesda, USA] |
− | * <b>GEO Datasets</b>, Gene Expression Omnibus:<br />[ | ||
* <b>modERN</b>, model organism Encyclopedia of Regulatory Networks:<br />[http://epic.gs.washington.edu/modERN/ modERN, University of Washington, Seattle, USA] | * <b>modERN</b>, model organism Encyclopedia of Regulatory Networks:<br />[http://epic.gs.washington.edu/modERN/ modERN, University of Washington, Seattle, USA] | ||
* <b>NCBI</b>, National Center for Biotechnology Information:<br />[http://www.ncbi.nlm.nih.gov Bethesda, MD USA] | * <b>NCBI</b>, National Center for Biotechnology Information:<br />[http://www.ncbi.nlm.nih.gov Bethesda, MD USA] | ||
* <b>NCBI Genome Data Viewer</b>, A genome view of Drosophila melanogaster:<br />[https://www.ncbi.nlm.nih.gov/genome/gdv/?org=drosophila-melanogaster NCBI, Bethesda, USA] | * <b>NCBI Genome Data Viewer</b>, A genome view of Drosophila melanogaster:<br />[https://www.ncbi.nlm.nih.gov/genome/gdv/?org=drosophila-melanogaster NCBI, Bethesda, USA] | ||
− | * <b>PubMed</b>, Biomedical literature full-text archive:<br />[ | + | * <b>PubMed</b>, Biomedical literature full-text archive:<br />[https://pubmed.ncbi.nlm.nih.gov/ PubMed, NCBI, Bethesda, USA] |
− | * <b>PubMed Central</b>, Biomedical literature citations and abstracts:<br />[ | + | * <b>PubMed Central</b>, Biomedical literature citations and abstracts:<br />[https://www.ncbi.nlm.nih.gov/pmc/ PubMed Central, NCBI, Bethesda, USA] |
− | * <b>SRA</b>, Sequence Read Archive:<br />[ | + | * <b>SRA</b>, Sequence Read Archive:<br />[https://www.ncbi.nlm.nih.gov/sra SRA, NCBI, Bethesda, MD, USA] |
* <b>UCSC Genome Browser Gateway</b> - D. melanogaster:<br />[http://genome.ucsc.edu/cgi-bin/hgGateway UCSC Genome Browser, UC Santa Cruz, Santa Cruz, USA] | * <b>UCSC Genome Browser Gateway</b> - D. melanogaster:<br />[http://genome.ucsc.edu/cgi-bin/hgGateway UCSC Genome Browser, UC Santa Cruz, Santa Cruz, USA] | ||
+ | |||
+ | =DNA Reagents= | ||
+ | * <b>Addgene Highly requested Drosophila plasmids</b>: <br />[https://www.addgene.org/search/catalog/pooled-libraries/?q=Drosophila Addgene, Watertown, MA, USA] | ||
+ | * <b>Addgene sgRNA Libraries</b>: Pooled sgRNA libraries for pooled CRISPR cell screening <br />[https://www.addgene.org/search/catalog/pooled-libraries/?q=Drosophila Addgene, Watertown, MA, USA] | ||
+ | * <b>DNASU Drosophila plasmids</b>: <br />[https://dnasu.org/DNASU/AdvancedSearchOptions.do?species=Drosophila%20melanogaster&psi=0 DNASU Plasmid Repository, Arizona State University, Tempe, AZ, USA] | ||
+ | * <b>DGRC DNA Clones Catalog</b>:<br />[https://dgrc.bio.indiana.edu/clones/Catalog Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC DNA Clone Collections</b>:<br />[https://dgrc.bio.indiana.edu/clones/BulkCollections Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC DNA Clone FAQ Page</b>:<br />[https://dgrc.bio.indiana.edu/FAQs?tab=clones Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC DNA Clone Protocols</b>:<br />[https://dgrc.bio.indiana.edu/Protocols?tab=clones Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC DNA Vectors Catalog</b>:<br />[https://dgrc.bio.indiana.edu/vectors/Catalog Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC DNA Vectors FAQs Page</b>:<br />[https://dgrc.bio.indiana.edu/FAQs?tab=vectors Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>DGRC DNA Vectors Protocols</b>:<br />[https://dgrc.bio.indiana.edu/Protocols?tab=vectors Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA] | ||
+ | * <b>TRiP: VALIUM and WALIUM plasmid vector sets</b>:<br />[https://fgr.hms.harvard.edu/trip-plasmid-vector-sets TRiP, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>TRiP: Plasmid cloning protocols</b>:<br />[https://fgr.hms.harvard.edu/cloning-and-sequencing TRiP, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>TRiP: Overview of sgRNA vectors</b>:<br />[https://fgr.hms.harvard.edu/sgrna-vectors TRiP, Harvard Medical School, Boston, MA, USA] | ||
=Drosophila Genomes Data and Metadata= | =Drosophila Genomes Data and Metadata= | ||
+ | * <b>DrosOmics</b>, a comparative genomics browser to explore omics data in 52 natural populations of D. melanogaster:<br />[https://www.biologiaevolutiva.org/gonzalez_lab/drosomics/ Institute of Evolutionary Biology, CSIC, Universitat Pompeu Fabra, Barcelona, Spain] | ||
* <b>DroSpeGe</b>, a view of Drosophila genome data, with genome maps and BLAST sequence search, for 12 species:<br />[http://insects.eugenes.org/species/ Genome Informatics Lab, Indiana University, Bloomington, Indiana, USA] | * <b>DroSpeGe</b>, a view of Drosophila genome data, with genome maps and BLAST sequence search, for 12 species:<br />[http://insects.eugenes.org/species/ Genome Informatics Lab, Indiana University, Bloomington, Indiana, USA] | ||
* <b>List of Drosophila reference genome assemblies</b> at FlyBase, NCBI and UCSC. [[File:DrosophilaGenomeAssemblies.xls]] (spreadsheet) ''Updated June 17, 2015'' | * <b>List of Drosophila reference genome assemblies</b> at FlyBase, NCBI and UCSC. [[File:DrosophilaGenomeAssemblies.xls]] (spreadsheet) ''Updated June 17, 2015'' | ||
− | * <b>''D. albomicans'' Genome</b>:<br />[ | + | * <b>''D. albomicans'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/2712/ ''D. albomicans'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/2712/ ''D. albomicans'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000298335 ''D. albomicans'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. americana'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/10917 ''D. americana'' Organism Overview, NCBI, USA]<br />[ | + | * <b>''D. americana'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/10917 ''D. americana'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/10917 ''D. americana'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_001245305.1 ''D. americana'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. ananassae'' Genome</b>:<br />[ | + | * <b>''D. ananassae'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/244/ ''D. ananassae'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/244/ ''D. ananassae'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005115 ''D. ananassae'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. arizonae'' Genome</b>:<br />[ | + | * <b>''D. arizonae'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/45090/ ''D. arizonae'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/45090/ ''D. arizonae'' Genome Assembly and Annotation Report, NCBI, USA]<br />[https://www.ebi.ac.uk/ena/data/view/GCA_001654025 ''D. arizonae'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. biarmipes'' Genome</b>:<br />[ | + | * <b>''D. biarmipes'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/3499/ ''D. biarmipes'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/3499/ ''D. biarmipes'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000233415 ''D. biarmipes'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. bipectinata'' Genome</b>:<br />[ | + | * <b>''D. bipectinata'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/3489/ ''D. bipectinata'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/3489/ ''D. bipectinata'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000236285 ''D. bipectinata'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. busckii'' Genome</b>:<br />[ | + | * <b>''D. busckii'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/38276/ ''D. busckii'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/38276/ ''D. busckii'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_001277935 ''D. busckii'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. erecta'' Genome</b>:<br />[ | + | * <b>''D. erecta'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/250/ ''D. erecta'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/250/ ''D. erecta'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005135 ''D. erecta'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. elegans'' Genome</b>:<br />[ | + | * <b>''D. elegans'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/3490 ''D. elegans'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/3490 ''D. elegans'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000224195 ''D. elegans'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. eugracilis'' Genome</b>:<br />[ | + | * <b>''D. eugracilis'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/6863/ ''D. eugracilis'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/6863/ ''D. eugracilis'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000236325 ''D. eugracilis'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. ficusphila'' Genome</b>:<br />[ | + | * <b>''D. ficusphila'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/3491/ ''D. ficusphila'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/3491/ ''D. ficusphila'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000220665 ''D. ficusphila'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. grimshawi'' Genome</b>:<br />[ | + | * <b>''D. grimshawi'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/257/ ''D. grimshawi'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/257/ ''D. grimshawi'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005155 ''D. grimshawi'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. kikkawai'' Genome</b>:<br />[ | + | * <b>''D. kikkawai'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/3492/ ''D. kikkawai'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/3492/ ''D. kikkawai'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000224215 ''D. kikkawai'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. melanogaster'' Genome</b>:<br />[ | + | * <b>''D. melanogaster'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/47/ ''D. melanogaster'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/47/ ''D. melanogaster'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000001215 ''D. melanogaster'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. miranda'' Genome</b>:<br />[ | + | * <b>''D. miranda'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/10915/ ''D. miranda'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/10915/ ''D. miranda'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000269505 ''D. miranda'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. mojavensis'' Genome</b>:<br />[ | + | * <b>''D. mojavensis'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/259/ ''D. mojavensis'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/259/ ''D. mojavensis'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005175 ''D. mojavensis'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. navojoa'' Genome</b>:<br />[ | + | * <b>''D. navojoa'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/45091/ ''D. navojoa'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/45091/ ''D. navojoa'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_001654015 ''D. navojoa'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. persimilis'' Genome</b>:<br />[ | + | * <b>''D. persimilis'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/265/ ''D. persimilis'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/265/ ''D. persimilis'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005195 ''D. persimilis'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. pseudoobscura'' Genome</b>:<br />[ | + | * <b>''D. pseudoobscura'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/219/ ''D. pseudoobscura'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/219/ ''D. pseudoobscura'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000001765 ''D. pseudoobscura'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. rhopaloa'' Genome</b>:<br />[ | + | * <b>''D. rhopaloa'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/6853/ ''D. rhopaloa'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/6853/ ''D. rhopaloa'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000236305 ''D. rhopaloa'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. sechellia'' Genome</b>:<br />[ | + | * <b>''D. sechellia'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/271/ ''D. sechellia'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/271/ ''D. sechellia'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005215 ''D. sechellia'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. simulans'' Genome</b>:<br />[ | + | * <b>''D. simulans'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/200/ ''D. simulans'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/200/ ''D. simulans'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000259045 ''D. simulans'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. suzukii'' Genome</b>:<br />[ | + | * <b>''D. suzukii'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/18317/ ''D. suzukii'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/18317/ ''D. suzukii'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000472105 ''D. suzukii'' Genome Assembly Report, ENA, UK]<br /> [http://spottedwingflybase.org/ ''D. suzukii'' SpottedWingFlyBase The Spotted Wing Drosophila Project, UC Davis and Oregon State University, USA] |
− | * <b>''D. takahashii'' Genome</b>:<br />[ | + | * <b>''D. takahashii'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/3493/ ''D. takahashii'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/3493/ ''D. takahashii'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000224235 ''D. takahashii'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. virilis'' Genome</b>:<br />[ | + | * <b>''D. virilis'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/261/ ''D. virilis'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/261/ ''D. virilis'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005245 ''D. virilis'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. willistoni'' Genome</b>:<br />[ | + | * <b>''D. willistoni'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/251/ ''D. willistoni'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/251/ ''D. willistoni'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005925 ''D. willistoni'' Genome Assembly Report, ENA, UK] |
− | * <b>''D. yakuba'' Genome</b>:<br />[ | + | * <b>''D. yakuba'' Genome</b>:<br />[https://www.ncbi.nlm.nih.gov/genome/148/ ''D. yakuba'' Organism Overview, NCBI, USA]<br />[https://www.ncbi.nlm.nih.gov/genome/genomes/148/ ''D. yakuba'' Genome Assembly Report, NCBI, USA]<br />[http://www.ebi.ac.uk/ena/data/view/GCA_000005975 ''D. yakuba'' Genome Assembly Report, ENA, UK]] |
=Gene Expression Databases and Tools= | =Gene Expression Databases and Tools= | ||
− | * <b>BDGP in situ</b>, Patterns of gene expression in Drosophila embryogenesis:<br />[ | + | * <b>BDGP in situ</b>, Patterns of gene expression in Drosophila embryogenesis:<br />[https://insitu.fruitfly.org/cgi-bin/ex/insitu.pl Gene expression database, BDGP, University of California, Berkeley, USA] |
− | * <b>BGee</b> Normal Gene Expression Data in Animals (based on RNA-Seq, Affymetrix, in situ hybridization, and EST data).<br />[ | + | * <b>BGee</b> Normal Gene Expression Data in Animals (based on RNA-Seq, Affymetrix, in situ hybridization, and EST data).<br />[https://www.bgee.org/ SIB, Swiss Institute of Bioinformatics, University of Lausanne, Lausanne, Switzerland] |
− | * <b>FlyAtlas2</b>, for exploring how genes are expressed in the tissues of ''D. melanogaster'':<br />[ | + | * <b>DIGITtally</b>, Finding Genes of Interest DIGITtally: <br /> [https://www.digittally.org/ DIGITtally, University of Glasgow, Glasgow, UK] |
+ | * <b>FlyAtlas2</b>, for exploring how genes are expressed in the tissues of ''D. melanogaster'':<br />[https://flyatlas.gla.ac.uk/FlyAtlas2/index.html FlyAtlas2, University of Glasgow, Glasgow, UK] | ||
* <b>FlyExpress</b>, an Expression Pattern Search Engine:<br />[http://www.flyexpress.net/ FlyExpress, Arizona State University, USA] | * <b>FlyExpress</b>, an Expression Pattern Search Engine:<br />[http://www.flyexpress.net/ FlyExpress, Arizona State University, USA] | ||
− | * <b>Fly-FISH</b>, A Database of Drosophila Embryo mRNA Localizaton Patterns:<br />[ | + | * <b>Fly-FISH</b>, A Database of Drosophila Embryo mRNA Localizaton Patterns:<br />[https://fly-fish.ccbr.utoronto.ca/ Fly-FISH, University of Toronto, Toronto, Canada] |
* <b>FlyGut-seq</b>, a transcriptomic resource that complements the original [http://flygut.epfl.ch/ Flygut website]:<br />[http://flygutseq.buchonlab.com/ Buchon Lab, Cornell University, Ithaca, NY, USA] | * <b>FlyGut-seq</b>, a transcriptomic resource that complements the original [http://flygut.epfl.ch/ Flygut website]:<br />[http://flygutseq.buchonlab.com/ Buchon Lab, Cornell University, Ithaca, NY, USA] | ||
− | * <b>FlyLight</b>, Expression Patterns of GAL4 Driver Lines:<br />[ | + | * <b>FlyLight</b>, Expression Patterns of GAL4, LexA, and Split-GAL4 Driver Lines to enable the visualization and precise manipulation of individual cell types in the Drosophila nervous system:<br />[https://www.janelia.org/project-team/flylight HHMI, Janelia Farms Research Campus] |
− | + | * <b>flytrap</b>, a database of P{GAL4} enhancer traps and their expression in brains: <br />[https://www.fly-trap.org/ flytrap, University of Edinburgh, UK] | |
− | * <b>flytrap</b>, a database of P{GAL4} enhancer traps and their expression in brains: <br />[ | + | * <b>Gene Expression Levels by Cell Line</b> (based on modENCODE data):<br />[https://www.flyrnai.org/cgi-bin/RNAi_expression_levels.pl DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>Gene Expression Levels by Cell Line</b> (based on modENCODE data):<br />[ | + | * <b>GEO Profiles</b>, Gene Expression Omnibus:<br />[https://www.ncbi.nlm.nih.gov/geo/info/profiles.html GEO, NCBI, Bethesda, MD USA] |
− | * <b>GEO Profiles</b>, Gene Expression Omnibus:<br />[ | ||
− | |||
* <b>MidgutAtlas</b>, Gene expression in five pH-defined regions of the larval midgut of D. melanogaster:<br />[http://flyatlas.gla.ac.uk/MidgutAtlas/index.html Dow laboratory, University of Glascow, Scotland, UK] | * <b>MidgutAtlas</b>, Gene expression in five pH-defined regions of the larval midgut of D. melanogaster:<br />[http://flyatlas.gla.ac.uk/MidgutAtlas/index.html Dow laboratory, University of Glascow, Scotland, UK] | ||
− | * <b>RNAMiner</b>, A Bioinformatics Protocol for Mining Large RNA-Seq Transcriptomics Data:<br />[ | + | * <b>RNAMiner</b>, A Bioinformatics Protocol for Mining Large RNA-Seq Transcriptomics Data:<br />[https://calla.rnet.missouri.edu/rnaminer/ Cheng Lab, University of Missouri, Mizzou, Columbia, MO, USA] |
=Gene Groups= | =Gene Groups= | ||
− | * <b>FlyBase Gene Groups</b>, Manually curated reports on functionally related genes, based on the literature:<br /> [ | + | * <b>FlyBase Gene Groups</b>, Manually curated reports on functionally related genes, based on the literature:<br /> [https://flybase.org/lists/FBgg/ Gene group list, FlyBase] |
− | * <b>FlyBase Signaling Pathways</b>, Manually curated reports on Signaling Pathways, based on the literature:<br /> [ | + | * <b>FlyBase Signaling Pathways</b>, Manually curated reports on Signaling Pathways, based on the literature:<br /> [https://flybase.org/lists/FBgg/pathways Pathways list, FlyBase] |
− | * <b>GLAD</b>, an Online Database of Gene List Annotations for Drosophila:<br /> [ | + | * <b>GLAD</b>, an Online Database of Gene List Annotations for Drosophila:<br /> [https://www.flyrnai.org/tools/glad/web/ DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>Interactive fly</b>, Drosophila genes listed by biochemical function:<br /> [ | + | * <b>Interactive fly</b>, Drosophila genes listed by biochemical function:<br /> [https://www.sdbonline.org/sites/fly/aimain/6biochem.htm Interactive Fly, Thomas Brody and Society for Developmental Biology, MD, USA] |
− | * <b>Interactive fly</b>, Maternally transcribed genes:<br /> [ | + | * <b>Interactive fly</b>, Maternally transcribed genes:<br /> [https://www.sdbonline.org/sites/fly/aimain/4matrnl.htm Interactive Fly, Thomas Brody and Society for Developmental Biology, MD, USA] |
− | * <b>Interactive fly</b>, Zygotically transcribed genes:<br /> [ | + | * <b>Interactive fly</b>, Zygotically transcribed genes:<br /> [https://www.sdbonline.org/sites/fly/aimain/5zygotic.htm Interactive Fly, Thomas Brody and Society for Developmental Biology, MD, USA] |
− | * <b>KEGG BRITE</b>, Functional hierarchies and binary relationships of biological entities:<br /> [ | + | * <b>KEGG BRITE</b>, Functional hierarchies and binary relationships of biological entities:<br /> [https://www.genome.jp/kegg/brite.html KEGG, Kyoto University, Kyoto, Japan and University of Tokyo, Tokyo, Japan] |
* <b>UCSC D. melanogaster Gene Sorter</b>, displays tables of genes related by a variety of characteristics including protein-level homology, similarity of gene expression profiles, and genomic proximity.:<br /> [https://genome.ucsc.edu/cgi-bin/hgNear?org=D.+melanogaster UCSC Genome Informatics Group, University of California, Santa Cruz, Santa Cruz, CA, USA] | * <b>UCSC D. melanogaster Gene Sorter</b>, displays tables of genes related by a variety of characteristics including protein-level homology, similarity of gene expression profiles, and genomic proximity.:<br /> [https://genome.ucsc.edu/cgi-bin/hgNear?org=D.+melanogaster UCSC Genome Informatics Group, University of California, Santa Cruz, Santa Cruz, CA, USA] | ||
=General Bioinformatics Tools= | =General Bioinformatics Tools= | ||
− | * <b> | + | * <b>Addgene</b>, Early Career Researcher Toolbox: Free Online Molecular Biology Tools:<br />[https://blog.addgene.org/free-online-molecular-biology-tools Addgene, Watertown, MA, USA] |
− | * <b> | + | * <b>ApE</b>, A plasmid Editor:<br />[https://jorgensen.biology.utah.edu/wayned/ape/ ApE, University of Utah, Salt Lake City, UT, USA] |
+ | * <b>Bioconductor</b>, Open Source Software for Bioinformatics:<br />[https://www.bioconductor.org/ Fred Hutchinson Cancer Research Center, Seattle, USA] | ||
* <b>Bioz</b>, Insights and recommendations for products, equipment and assays:<br />[https://www.bioz.com/search/Drosophila Palo Alto, CA, USA] | * <b>Bioz</b>, Insights and recommendations for products, equipment and assays:<br />[https://www.bioz.com/search/Drosophila Palo Alto, CA, USA] | ||
− | * <b>Cytoscape</b> -- Network Data Integration, Analysis, and Visualization in a Box:<br />[ | + | * <b>Cytoscape</b> -- Network Data Integration, Analysis, and Visualization in a Box:<br />[https://cytoscape.org/ Cytoscape, Cytoscape Consortium, Canada, France, and USA] |
− | + | * <b>FlyMine</b>, an Integrated Database for Drosophila and Anopheles Genomics:<br />[https://www.flymine.org/ University of Cambridge, United Kingdom] | |
− | * <b>FlyMine</b>, an Integrated Database for Drosophila and Anopheles Genomics:<br />[ | ||
* <b>FlyNet</b>, a network prioritization server for Drosophila melanogaster biology:<br />[http://www.inetbio.org/flynet/ NetBioLab, Yonsei University, Seoul, Korea] | * <b>FlyNet</b>, a network prioritization server for Drosophila melanogaster biology:<br />[http://www.inetbio.org/flynet/ NetBioLab, Yonsei University, Seoul, Korea] | ||
− | * <b> | + | |
− | * <b>GSEA</b>, Gene Set Enrichment Analysis:<br />[ | + | =Gene Set Enrichment Analysis= |
+ | |||
+ | A summary table of selected GSEA tools with information about set up can be found [[FlyBase:GSEA|<b>here</b>]] | ||
+ | |||
+ | * <b>DAVID</b>, Database for Annotation, Visualization and Integrated Discovery :<br />[https://david.ncifcrf.gov/ DAVID, Laboratory of Human Retrovirology and Immunoinformatics, Frederick, MD, USA]] | ||
+ | * <b>Enrichr</b>, Human and Mouse Gene Set Enrichment Analysis:<br />[https://maayanlab.cloud/Enrichr/ Enrichr, Center for Bioinfomatics, Mount Sinai, New York, NY, USA] | ||
+ | * <b>MOET</b>, Multi Ontology Enrichment Tool:<br />[https://rgd.mcw.edu/rgdweb/enrichment/start.html MOET, Rat Genome Database] | ||
+ | * <b>modEnrichr</b>, Model Organism Gene Set Enrichment Analysis:<br />[https://maayanlab.cloud/modEnrichr/ modEnrichr, Center for Bioinfomatics, Mount Sinai, New York, NY, USA] | ||
+ | * <b>flyEnrichr</b>, D.melanogaster Gene Set Enrichment Analysis:<br />[https://maayanlab.cloud/FlyEnrichr/ flyEnrichr, Center for Bioinfomatics, Mount Sinai, New York, NY, USA] | ||
+ | * <b>GO term finder</b>, Generic GO Term Finder:<br />[https://go.princeton.edu/cgi-bin/GOTermFinder GOTERMFINDER, Lewis-Sigler Institute for Integrative Genomics, Princeton University, New Jersey, NJ, USA] | ||
+ | * <b>GOC</b>, GO Enrichment Analysis:<br />[https://geneontology.org/ Gene Ontology Consortium] | ||
+ | * <b>GSEA</b>, Gene Set Enrichment Analysis:<br />[https://www.gsea-msigdb.org/gsea/index.jsp GSEA, Broad Institute, Cambridge, MA, USA] | ||
+ | * <b>g:Profiler</b>, g:Profiler/g:GOSt:<br />[https://biit.cs.ut.ee/gprofiler/gost g:Profiler, Institute of Computer Science, University of Tartu, Tartu, Estonia] | ||
+ | * <b>GOrilla</b>, Gene Ontology enRIchment anaLysis and visuaLizAtion tool:<br />[https://cbl-gorilla.cs.technion.ac.il/ GOrilla, Technion, Israel Institute of Technology, Israel] | ||
+ | * <b>PANGEA</b>, PAthway, Network and Gene-set Enrichment Analysis:<br />[https://www.flyrnai.org/tools/pangea/ PANGEA, DRSC, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>PANTHER</b>, PANTHER GO Enrichment Analysis:<br />[https://pantherdb.org/ PANTHER, University of Southern California, Los Angeles, CA, USA] | ||
+ | * <b>REVIGO</b>, Reduce + Visualize Gene Ontology:<br />[http://revigo.irb.hr/ REVIGO, Division of electronics, Ruđer Bošković Institute, Zagreb, Croatia] | ||
+ | * <b>ShinyGO</b>, ShinyGO 0.80:<br />[http://bioinformatics.sdstate.edu/go80/ ShinyGO, South Dakota State University, Brookings, SD, USA] | ||
+ | * <b>WebGestalt</b>, WebGestalt Gene Set Enrichment Analysis:<br />[https://www.webgestalt.org/ WebGestalt, Baylor College of Medicine, Houston, TX, USA] | ||
=Genome Sequencing Projects= | =Genome Sequencing Projects= | ||
* <b>''D. pseudoobscura'' genome project</b>:<br />[https://www.hgsc.bcm.edu/arthropods/drosophila-pseudoobscura-genome-project D. pseudoobscura Human Genome Sequencing Center, Baylor College of Medicine, USA] | * <b>''D. pseudoobscura'' genome project</b>:<br />[https://www.hgsc.bcm.edu/arthropods/drosophila-pseudoobscura-genome-project D. pseudoobscura Human Genome Sequencing Center, Baylor College of Medicine, USA] | ||
− | |||
=Human Disease: Drosophila Models and Orthologous Genes= | =Human Disease: Drosophila Models and Orthologous Genes= | ||
+ | * <b>AGR</b> -- Alliance of Genome Resources:<br />[https://www.alliancegenome.org/ Alliance of Genome Resources, RGD, MGD, ZFIN, FlyBase, WormBase, SGD, GO consortium, USA and UK] | ||
* <b>Bloomington Stock Center</b>, Drosophila and Human Disease page:<br />[https://bdsc.indiana.edu/stocks/hd/index.html Bloomington Stock Center, Bloomington, IN, USA] | * <b>Bloomington Stock Center</b>, Drosophila and Human Disease page:<br />[https://bdsc.indiana.edu/stocks/hd/index.html Bloomington Stock Center, Bloomington, IN, USA] | ||
* <b>DIOPT-DIST</b>, Disease-related Ortholog Tool:<br />[http://www.flyrnai.org/cgi-bin/DRSC_DG_query.pl DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] | * <b>DIOPT-DIST</b>, Disease-related Ortholog Tool:<br />[http://www.flyrnai.org/cgi-bin/DRSC_DG_query.pl DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] | ||
− | * <b>Drosophila as a Model for Human Diseases</b>:<br />[ | + | * <b>Drosophila as a Model for Human Diseases</b>:<br />[https://www.sdbonline.org/sites/fly/modelsystem/aamodelsystem.htm Interactive Fly, Bethesda, MD, USA] |
* <b>Drosophila Models of Human Disease</b>, a blog by Stephanie Mohr and Annette Parks:<br />[http://flydiseasemodels.blogspot.com/ flydiseasemodels.blogspot.com/] <br /> | * <b>Drosophila Models of Human Disease</b>, a blog by Stephanie Mohr and Annette Parks:<br />[http://flydiseasemodels.blogspot.com/ flydiseasemodels.blogspot.com/] <br /> | ||
* <b>FlyNet Human Disease Prioritization</b>:<br /> [http://www.inetbio.org/flynet/Network_fly_comm.php NetBioLab, Yonsei University, Seoul, Korea] | * <b>FlyNet Human Disease Prioritization</b>:<br /> [http://www.inetbio.org/flynet/Network_fly_comm.php NetBioLab, Yonsei University, Seoul, Korea] | ||
+ | * <b>Gene2Function</b>:<br /> [http://www.gene2function.org/search/ Gene2Function, Harvard Medical School, Boston, MA, USA] | ||
* <b>HuDis</b>, high confidence human disease gene - fly gene ortholog pairs:<br />[https://fgr.hms.harvard.edu/hudis-trip-fly-stocks DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | * <b>HuDis</b>, high confidence human disease gene - fly gene ortholog pairs:<br />[https://fgr.hms.harvard.edu/hudis-trip-fly-stocks DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] | ||
+ | * <b>MARRVEL</b>, Model organism Aggregated Resources for Rare Variant ExpLoration:<br />[http://marrvel.org/ MARRVEL, Baylor College of Medecine, Houston, TX, USA] | ||
* <b>MORPHIN</b>, Model ORganism Projected on a Human Integrated gene Network:<br />[http://www.inetbio.org/morphin/index.php MORPHIN, Lee Lab, Yonsei University, Korea and Marcotte Lab, University of Texas at Austin, USA.] | * <b>MORPHIN</b>, Model ORganism Projected on a Human Integrated gene Network:<br />[http://www.inetbio.org/morphin/index.php MORPHIN, Lee Lab, Yonsei University, Korea and Marcotte Lab, University of Texas at Austin, USA.] | ||
=Interaction and Pathway Databases= | =Interaction and Pathway Databases= | ||
− | * <b>BioGRID</b>, The General Repository for Interaction Datasets:<br />[ | + | * <b>BioCyc - FLY</b> -- Curated Drosophila melanogaster Pathways:<br />[https://biocyc.org/FLY/organism-summary?object=FLY FlyCyc, Harvard University, Cambridge, USA] |
− | * <b>COMPLEAT</b>, protein COMPLex Enrichment Analysis Tool:<br />[ | + | * <b>BioGRID</b>, The General Repository for Interaction Datasets:<br />[https://thebiogrid.org/index.php BioGRID, Mount Sinai Hospital, Toronto, Canada] |
− | + | * <b>COMPLEAT</b>, protein COMPLex Enrichment Analysis Tool:<br />[https://www.flyrnai.org/compleat/ COMPLEAT, DRSC, Harvard Medical School, Boston, MA, USA] | |
* <b>DPiM</b>, Drosophila Protein interaction Map:<br/>[https://interfly.med.harvard.edu/ DPiM, Harvard University Medical School, Boston, MA, USA] | * <b>DPiM</b>, Drosophila Protein interaction Map:<br/>[https://interfly.med.harvard.edu/ DPiM, Harvard University Medical School, Boston, MA, USA] | ||
* <b>DroID</b>, Drosophila Interactions Database:<br />[http://www.droidb.org/ Drosophila Interaction Database, Detroit, USA] | * <b>DroID</b>, Drosophila Interactions Database:<br />[http://www.droidb.org/ Drosophila Interaction Database, Detroit, USA] | ||
* <b>FlyBase Signaling Pathways</b>, Manually curated reports on Signaling Pathways, based on the literature:<br /> [http://flybase.org/lists/FBgg/pathways Pathways list, FlyBase] | * <b>FlyBase Signaling Pathways</b>, Manually curated reports on Signaling Pathways, based on the literature:<br /> [http://flybase.org/lists/FBgg/pathways Pathways list, FlyBase] | ||
− | |||
* <b>FlyNet Gene Prioritization</b>: <br /> [http://www.inetbio.org/flynet/Network_gene_prioritization.php NetBioLab, Yonsei University, Seoul, Korea] | * <b>FlyNet Gene Prioritization</b>: <br /> [http://www.inetbio.org/flynet/Network_gene_prioritization.php NetBioLab, Yonsei University, Seoul, Korea] | ||
* <b>FlyOde</b>, FLY Organ Developmental Network: <br /> [http://flyode.boun.edu.tr/index.html FlyOde, Bogazici University, Istanbul, Turkey] | * <b>FlyOde</b>, FLY Organ Developmental Network: <br /> [http://flyode.boun.edu.tr/index.html FlyOde, Bogazici University, Istanbul, Turkey] | ||
* <b>HOMER</b>, Software for motif discovery and next-gen sequencing analysis: <br /> [http://homer.ucsd.edu/homer/motif/ HOMER, University of California at San Diego, San Diego, CA, USA] | * <b>HOMER</b>, Software for motif discovery and next-gen sequencing analysis: <br /> [http://homer.ucsd.edu/homer/motif/ HOMER, University of California at San Diego, San Diego, CA, USA] | ||
* <b>IM Browser</b>, Drosophila Interactions Database:<br />[http://proteome.wayne.edu/PIMdb.html IM Browser, Wayne State University, Detroit, MI, USA] | * <b>IM Browser</b>, Drosophila Interactions Database:<br />[http://proteome.wayne.edu/PIMdb.html IM Browser, Wayne State University, Detroit, MI, USA] | ||
− | * <b>IntAct</b>, Molecular Interaction Database:<br />[ | + | * <b>IntAct</b>, Molecular Interaction Database:<br />[https://www.ebi.ac.uk/intact/ IntAct, EMBL-EBI, Hinxton, UK] |
− | * <b>KEGG</b> -- Kyoto Encyclopedia of Genes and Genomes:<br />[ | + | * <b>KEGG</b> -- Kyoto Encyclopedia of Genes and Genomes:<br />[https://www.genome.jp/kegg/ KEGG, Kyoto, Japan] |
− | * <b>Reactome Pathway Database</b>:<br />[ | + | * <b>MIST</b> -- Molecular Interactions Search Tool:<br />[https://fgrtools.hms.harvard.edu/MIST/ MIST, Harvard Medical School, Boston, MA, USA] |
− | * <b> | + | * <b>Reactome Pathway Database</b>:<br />[https://reactome.org/ Reactome, OICR, Ontario, Canada, EMBL-EBI, UK, NYU Medical Center, NY, USA] |
− | * <b>STRING</b>, Known and Predicted Protein-Protein Interactions<br />[ | + | * <b>SignaLink</b>, an integrated resource to analyze signaling pathway cross-talks, transcription factors, miRNAs and regulatory enzymes.:<br />[http://signalink.org/ SignaLink, Earlham Institute, Norwich, UK] |
− | * <b>WikiPathways</b>, an open, public platform dedicated to the curation of biological pathways by and for the scientific community:<br />[ | + | * <b>STRING</b>, Known and Predicted Protein-Protein Interactions<br />[https://string-db.org/ STRING, SIB, Switzerland CPR, Denmark, and EMBL, Heidelberg, Germany] |
+ | * <b>WikiPathways</b>, an open, public platform dedicated to the curation of biological pathways by and for the scientific community:<br />[https://www.wikipathways.org/index.php/WikiPathways WikiPathways, Gladstone Institute, San Francisco, CA, USA and Maastricht University, The Netherlands] | ||
− | = | + | =Metabolomics= |
− | * <b> | + | * <b>FlyMet</b>, A Tissue- and Sex-Specific Metabolomic Atlas and Database :<br />[http://www.flymet.org/ FlyMet, University of Glasgow, Glasgow, Scotland] |
=Miscellaneous= | =Miscellaneous= | ||
+ | * <b>AGR</b> -- Alliance of Genome Resources:<br />[https://www.alliancegenome.org/ Alliance of Genome Resources, RGD, MGD, ZFIN, FlyBase, WormBase, SGD, GO consortium, USA and UK] | ||
+ | * <b>BioLitMine</b>, Biological Literature Mining Tool for Human and Model Organisms:<br />[https://www.flyrnai.org/tools/biolitmine/web/ BioLitMine, Harvard Medical School, Boston, MA, USA] | ||
* <b>Bionet Archives</b>, a collection of bionet USENET newsgroups and parallel e-mail lists regarding Drosophila:<br />[http://www.bio.net/hypermail/dros/ Genome Informatics Lab, Indiana University, Bloomington, Indiana, USA] | * <b>Bionet Archives</b>, a collection of bionet USENET newsgroups and parallel e-mail lists regarding Drosophila:<br />[http://www.bio.net/hypermail/dros/ Genome Informatics Lab, Indiana University, Bloomington, Indiana, USA] | ||
− | + | * <b>Drosophila App</b>, A mobile application developed by Genesee for use in fly research: <br />[https://play.google.com/store/apps/details?id=kaver.co.drosophila&hl=en Android] | |
− | * <b>Drosophila App</b>, A mobile application developed by Genesee for use in fly research: <br /> | + | * <b>Drosophila Information Service</b>:<br />[https://www.ou.edu/journals/dis/ DIS, Norman, Oklahoma, USA] |
− | * <b>Drosophila Information Service</b>:<br />[ | + | * <b>Drosophila Species Photographs</b>, Photographs of 133 species of Drosophila:<br />[https://kyotofly.kit.jp/cgi-bin/ehime/photo_data_list.cgi Ehime University, Matsuyama, Japan] |
− | * <b>Drosophilids of the Midwest and Northeast</b>:<br />[https://digitalcommons.mtu.edu/oabooks/1/ by T. Werner, Michigan Technological University and J.Jaenike, University of Rochester] | + | * <b>Drosophila Workers Unite! A laboratory manual for working with Drosophila</b>:<br />[https://marksteinlab.org/dwu/ Michele Markstein Lab, UMass Amherst, Amherst, MA, USA] |
+ | * <b>The Encyclopedia of North American Drosophilids Volume 1: Drosophilids of the Midwest and Northeast</b>:<br />[https://digitalcommons.mtu.edu/oabooks/1/ by T. Werner, Michigan Technological University, Tessa Steenwinkel, Michigan Technological University, and J.Jaenike, University of Rochester] | ||
* <b>Fly Art</b> - A compendium of arty imagery, sculptures or poetry inspired by Drosophila or flies in the wider sense.:<br />[https://droso4public.wordpress.com/fly-art/ University of Manchester, Manchester, UK] | * <b>Fly Art</b> - A compendium of arty imagery, sculptures or poetry inspired by Drosophila or flies in the wider sense.:<br />[https://droso4public.wordpress.com/fly-art/ University of Manchester, Manchester, UK] | ||
− | * <b>Fly Labs and References</b>, a large list of fly labs and recent publications: <br />[ | + | * <b>Fly Labs and References</b>, a large list of fly labs and recent publications: <br />[https://www.sdbonline.org/sites/fly/flylabs/a.html Interactive Fly, Bethesda, MD, USA] |
− | * <b> | + | * <b>FlyTree</b>, the Drosophila Researcher Genealogy has been incorporated into the Academic Tree Project: <br />[https://academictree.org/flytree/ FlyTree, USA] |
− | * <b> | + | * <b>Fully automated drosophila wing landmarking</b>, Automatically finds more than 110 landmark points and creates a digital twin of the drosophila wing:<br />[https://datamarkin.com/models/drosophila-wings-beta/ Datamarkin, Marseille, France] |
− | * <b>GenAge</b> -- The Ageing Gene Database:<br />[ | + | * <b>GenAge</b> -- The Ageing Gene Database:<br />[https://genomics.senescence.info/genes/index.html Human Ageing Genomic Resources, Institute of Integrative Biology, Liverpool, UK] |
− | * <b>Gene Lookup</b>, Gene and Reagent Lookup:<br />[ | + | * <b>Gene Lookup</b>, Gene and Reagent Lookup:<br />[https://www.flyrnai.org/cgi-bin/DRSC_gene_lookup.pl Gene Lookup, DRSC, Harvard Medical School, Boston, MA, USA] |
− | * <b> | + | * <b>GlyCosmos Portal</b>, a Web portal aiming to integrate the glycosciences with the life sciences. It consists of Standards, Repositories and Data Resources, providing information about genes, proteins, lipids, pathways and diseases.:<br />[https://glycosmos.org/ GlyCosmos, GlyCosmos is a member of the GlySpace Alliance, consisting of ExPASy Glycomics, GlyCosmos, and GlyGen] |
− | * <b>Interactive Fly</b>, A cyberspace guide to Drosophila development and metazoan evolution<br />[ | + | * <b>Interactive Fly</b>, A cyberspace guide to Drosophila development and metazoan evolution<br />[https://www.sdbonline.org/sites/fly/aimain/1aahome.htm Interactive Fly, Bethesda, MD, USA] |
− | |||
* <b>Manchester Fly Facility, Resources</b> - A compendium of information on ''Drosophila melanogaster'' as a model organism:<br />[https://droso4public.wordpress.com/resources/ University of Manchester, Manchester, UK] | * <b>Manchester Fly Facility, Resources</b> - A compendium of information on ''Drosophila melanogaster'' as a model organism:<br />[https://droso4public.wordpress.com/resources/ University of Manchester, Manchester, UK] | ||
− | |||
− | |||
* <b>Multiplex Fluorescent mRNA <em>In Situ</em> Hybridization</b>, information on how to perform multiplex fluorescent mRNA in situ hybridization on Drosophila embryos: <br />[http://people.biology.ucsd.edu/davek/ Bier and McGinnis Labs, UCSD, San Diego, California, USA] | * <b>Multiplex Fluorescent mRNA <em>In Situ</em> Hybridization</b>, information on how to perform multiplex fluorescent mRNA in situ hybridization on Drosophila embryos: <br />[http://people.biology.ucsd.edu/davek/ Bier and McGinnis Labs, UCSD, San Diego, California, USA] | ||
− | + | * <b>What's hot today:</b> Current papers in developmental biology and gene function:<br />[https://www.sdbonline.org/sites/fly/aimain/model-organisms-this-week.htm Interactive Fly, Bethesda, MD, USA] | |
− | * <b>What's hot today:</b> Current papers in developmental biology and gene function:<br />[ | + | * <b>The WWW Virtual Library--Drosophila</b>:<br />[https://www.ceolas.org/fly/ Drosophila Virtual Library] |
− | * <b>The WWW Virtual Library--Drosophila</b>:<br />[ | ||
=Non-coding (ncRNA) Databases and Tools= | =Non-coding (ncRNA) Databases and Tools= | ||
==General== | ==General== | ||
− | * <b>NONCODE</b>, An integrated knowledge database dedicated to ncRNAs, especially lncRNAs | + | * <b>NONCODE</b>, An integrated knowledge database dedicated to ncRNAs, especially lncRNAs:<br />[http://www.noncode.org/ NONCODE, Tsinghua University and Chinese Academy of Sciences, Beijing, China] |
− | + | * <b>Rfam</b>, A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements :<br />[https://rfam.org/ Rfam, EMBL-EBI, Hinxton, UK] | |
− | * <b>Rfam</b>, A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements | + | * <b>RNAcentral</b>, Integrated access to a comprehensive and up-to-date set of non-coding RNA sequences provided by a collaborating group of Expert Databases :<br />[https://rnacentral.org/ RNAcentral, EMBL-EBI, Hinxton, UK] |
− | * <b>RNAcentral</b>, Integrated access to a comprehensive and up-to-date set of non-coding RNA sequences provided by a collaborating group of Expert Databases | ||
==lncRNA== | ==lncRNA== | ||
− | * <b> | + | * <b>LncRBase V.2</b>, a database of information about lncRNAs from 8 species (human, mouse, fly, zebrafish, rat, chicken, C.elegans and cow) :<br />[http://dibresources.jcbose.ac.in/zhumur/lncrbase2/index_Contact.php LncRBase, Bose Institute, West Bengal, India] |
==miRNA== | ==miRNA== | ||
===General=== | ===General=== | ||
− | * <b>miRBase</b>, A searchable database of published miRNA sequences and annotations (current miBase release is 22 - last updated 2018):<br />[ | + | * <b>miRBase</b>, A searchable database of published miRNA sequences and annotations (current miBase release is 22 - last updated 2018):<br />[https://mirbase.org/// miRBase, University of Manchester, UK]<br /> |
::NOTE - mirBase Release 21 (June 2014) uses old reference genome assemblies for D. melanogaster (Dmel_Release_5), D. simulans (Dsim_Release_1) and D. pseudoobscura (Dpse_Release_2). Linkouts from D. melanogaster miRNA genes to the Ensembl Genome browser (which displays Dmel_Release_6) will point to the old "Release_5" coordinates, which will be off the mark for many genes, especially those on X, 2R, 3R and 4 chromosomes. | ::NOTE - mirBase Release 21 (June 2014) uses old reference genome assemblies for D. melanogaster (Dmel_Release_5), D. simulans (Dsim_Release_1) and D. pseudoobscura (Dpse_Release_2). Linkouts from D. melanogaster miRNA genes to the Ensembl Genome browser (which displays Dmel_Release_6) will point to the old "Release_5" coordinates, which will be off the mark for many genes, especially those on X, 2R, 3R and 4 chromosomes. | ||
* <b>miRNEST</b>, An integrated collection of miRNA predictions from high-throughput sequencing experiments and external database annotations (last updated 2015):<br />[http://rhesus.amu.edu.pl/mirnest/copy/ miRNEST, The Adam Mickiewicz University in Poznan, Poznan, Poland] | * <b>miRNEST</b>, An integrated collection of miRNA predictions from high-throughput sequencing experiments and external database annotations (last updated 2015):<br />[http://rhesus.amu.edu.pl/mirnest/copy/ miRNEST, The Adam Mickiewicz University in Poznan, Poznan, Poland] | ||
− | |||
* <b>TransmiR</b>, A database of transcription factor-microRNA regulation (last updated 2018):<br />[http://www.cuilab.cn/transmir TransmiR], Peking University Health Science Center, Peking, Chin | * <b>TransmiR</b>, A database of transcription factor-microRNA regulation (last updated 2018):<br />[http://www.cuilab.cn/transmir TransmiR], Peking University Health Science Center, Peking, Chin | ||
===miRNA Target Prediction=== | ===miRNA Target Prediction=== | ||
− | * <b>DIANA microT-CDS</b>, MicroRNA Target Prediction:<br />[ | + | * <b>DIANA microT-CDS</b>, MicroRNA Target Prediction:<br />[https://dianalab.e-ce.uth.gr/html/dianauniverse/index.php?r=microT_CDS DIANA Tools, DIANA LAB, Athens, Greece] |
− | + | * <b>MinoTar</b>, Predict microRNA Targets in Coding Sequence (last updated 2010):<br />[https://www.flyrnai.org/cgi-bin/DRSC_MinoTar.pl MinoTar, DRSC, Harvard Medical School, Boston, MA, USA] | |
− | * <b>MinoTar</b>, Predict microRNA Targets in Coding Sequence (last updated 2010):<br />[ | + | * <b>TargetScanFly</b>, Prediction of microRNA targets based on miRNA seed region and target conservation (last updated 2012):<br />[https://www.targetscan.org/fly_12/ TargetScanFly, Whitehead Institute and MIT Dept. of Biology, Cambridge, MA, USA] |
− | |||
− | * <b>TargetScanFly</b>, Prediction of microRNA targets based on miRNA seed region and target conservation (last updated 2012):<br />[ | ||
===Validated miRNA Targets=== | ===Validated miRNA Targets=== | ||
− | * <b>DIANA TarBase</b>, MicroRNA Target Database (last updated 2017):<br />[ | + | * <b>DIANA TarBase</b>, MicroRNA Target Database (last updated 2017):<br />[https://dianalab.e-ce.uth.gr/html/diana/web/index.php?r=tarbasev8 DIANA TarBase, DIANA LAB, Athens, Greece] |
− | + | * <b>miRTex</b>, A text-mining system that extracts miRNA-gene regulation relations and miRNA-target relations from literature (last updated 2017):<br />[https://research.bioinformatics.udel.edu/miRTex/ miRTex, University of Delaware, Newark, DE, USA] | |
− | * <b>miRTex</b>, A text-mining system that extracts miRNA-gene regulation relations and miRNA-target relations from literature (last updated 2017):<br />[ | ||
===Comparative miRNA Analysis=== | ===Comparative miRNA Analysis=== | ||
− | |||
* <b>microRNAviewer</b>, A global view of homologous miRNA genes in many species (last updated 2012):<br />[http://people.csail.mit.edu/akiezun/microRNAviewer/index.html microRNAviewer, Tel Aviv University, Tel Aviv, Israel] | * <b>microRNAviewer</b>, A global view of homologous miRNA genes in many species (last updated 2012):<br />[http://people.csail.mit.edu/akiezun/microRNAviewer/index.html microRNAviewer, Tel Aviv University, Tel Aviv, Israel] | ||
− | |||
==rRNA== | ==rRNA== | ||
− | * <b>SILVA</b>, High quality ribosomal RNA databases (last updated | + | * <b>SILVA</b>, High quality ribosomal RNA databases (last updated 2020):<br />[https://www.arb-silva.de/ SILVA, Max Planck Institute for Marine Microbiology and Jacobs University, Bremen, Germany] |
==tRNA== | ==tRNA== | ||
− | |||
* <b>tRFdb</b>, A relational database of Transfer RNA related Fragments:<br />[http://genome.bioch.virginia.edu/trfdb/index.php tRFdb, Dutta lab, University of Virginia, Charlottesville, VA, USA] | * <b>tRFdb</b>, A relational database of Transfer RNA related Fragments:<br />[http://genome.bioch.virginia.edu/trfdb/index.php tRFdb, Dutta lab, University of Virginia, Charlottesville, VA, USA] | ||
Line 229: | Line 265: | ||
=Ontology Resources= | =Ontology Resources= | ||
− | |||
* <b>BioPortal</b>, a biomedical ontologies repository and browser:<br />[http://bioportal.bioontology.org/ BioPortal, The National Center for Biomedical Ontology, USA] | * <b>BioPortal</b>, a biomedical ontologies repository and browser:<br />[http://bioportal.bioontology.org/ BioPortal, The National Center for Biomedical Ontology, USA] | ||
− | * <b>Disease Ontology</b>, ontology of disease terms:<br />[http://disease-ontology.org/ Disease Ontology] | + | * <b>Disease Ontology (DO)</b>, ontology of disease terms:<br />[http://disease-ontology.org/ Disease Ontology] |
− | * <b>Gene Ontology Consortium</b><br />[ | + | * <b>Gene Ontology Consortium (GO)</b><br />[https://geneontology.org/ Gene Ontology Consortium] |
− | * <b>GOrilla</b> -- Gene Ontology enRIchment and anaLysis and viuaLizAtion tool:<br />[ | + | * <b>GOrilla</b> -- Gene Ontology enRIchment and anaLysis and viuaLizAtion tool:<br />[https://cbl-gorilla.cs.technion.ac.il/ GOrilla, Israel] |
* <b>OboEdit</b>, browser for OBO ontologies:<br />[http://oboedit.org/ OboEdit] | * <b>OboEdit</b>, browser for OBO ontologies:<br />[http://oboedit.org/ OboEdit] | ||
− | * <b>The OBO Foundry</b>, an ontology registry:<br />[http://obofoundry. | + | * <b>The OBO Foundry</b>, an ontology registry:<br />[http://obofoundry.org/ OboFoundry] |
− | * <b>Ontobee</b>, an ontologies browser:<br />[ | + | * <b>Ontobee</b>, an ontologies browser:<br />[https://ontobee.org/ Ontobee, He Group, University of Michigan Medical School, Ann Arbor, Michigan, USA] |
− | * <b>Ontology Lookup Service</b>, an ontologies browser:<br />[ | + | * <b>OLS - Ontology Lookup Service</b>, an ontologies browser:<br />[https://www.ebi.ac.uk/ols4/ Ontology Lookup Service, EMBL-EBI, Hinxton, UK] |
− | * <b>Protege</b>, browser for OWL ontologies:<br />[ | + | * <b>Protege</b>, browser for OWL ontologies:<br />[https://protege.stanford.edu/ Protege, Stanford Center for Biomedical Informatics Research, Stanford University School of Medicine, USA] |
− | * <b>QuickGO</b>, a browser for Gene Ontology terms and annotations:<br />[ | + | * <b>QuickGO</b>, a browser for Gene Ontology terms and annotations:<br />[https://www.ebi.ac.uk/QuickGO/ QuickGO, EMBL-EBI, Hinxton, UK] |
=Orthology Predictions= | =Orthology Predictions= | ||
* <b>Compara</b>, Ensembl Compara provides cross-species resources and analyses, at both the sequence level and the gene level.<br />[http://useast.ensembl.org/info/genome/compara/index.html?redirect=no Ensembl, Hinxton, UK] | * <b>Compara</b>, Ensembl Compara provides cross-species resources and analyses, at both the sequence level and the gene level.<br />[http://useast.ensembl.org/info/genome/compara/index.html?redirect=no Ensembl, Hinxton, UK] | ||
− | * <b>DIOPT</b>, DRSC Integrative Ortholog Prediction Tool<br />[ | + | * <b>DIOPT</b>, DRSC Integrative Ortholog Prediction Tool<br />[https://www.flyrnai.org/cgi-bin/DRSC_orthologs.pl DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>DIOPT-DIST: DIOPT Diseases and Traits</b>, DRSC Disease Gene Query Tool:<br />[ | + | * <b>DIOPT-DIST: DIOPT Diseases and Traits</b>, DRSC Disease Gene Query Tool:<br />[https://www.flyrnai.org/cgi-bin/DRSC_DG_query.pl DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>EggNOG</b>, A database of orthologous groups and functional annotation:<br />[http://eggnogdb.embl.de/ | + | * <b>EggNOG</b>, A database of orthologous groups and functional annotation:<br />[http://eggnogdb.embl.de/ Computational Biology Group, EMBL, Heidelberg, Germany] |
− | * <b>Homologene</b>, An automated system for constructing putative homology groups from the complete gene sets of a wide range of eukaryotic species.<br />[ | + | * <b>Gene2Function</b>:<br /> [https://www.gene2function.org/search/ Gene2Function, Harvard Medical School, Boston, MA, USA] |
− | * <b> | + | * <b>Hieranoid</b>, An orthology inference method using a hierarchical approach. Hieranoid performs pairwise orthology analysis using InParanoid at each node in a guide tree as it progresses from its leaves to the root.<br />[https://hieranoid.sbc.su.se/index.html Hieranoid, Stockholm Bioinformatics Centre, Stockholm, Sweden] |
− | * <b>Isobase</b>, A Database of Functionally Related Orthologs, which incorporates PPI network data in addition to sequence similarity.<br />[http:// | + | * <b>HieranoiDB</b>, An online database of orthologs inferred by Hieranoid 2 for a representative set of proteomes.<br />[https://hieranoidb.sbc.su.se/ HieranoiDB, Stockholm Bioinformatics Centre, Stockholm, Sweden] |
− | * <b>OMA Browser</b>, The OMA (“Orthologous MAtrix”) project is a method and database for the inference of orthologs among complete genomes.<br />[ | + | * <b>Homologene</b>, An automated system for constructing putative homology groups from the complete gene sets of a wide range of eukaryotic species.<br />[https://www.ncbi.nlm.nih.gov/datasets/gene/ NCBI, Bethesda, MD, USA] |
+ | * <b>InParanoiDB 9</b>, Ortholog groups with inparalogs for proteins and protein domains<br />[https://inparanoidb.sbc.su.se/about/ InParanoiDB 9, Stockholm Bioinformatics Centre, Stockholm, Sweden] | ||
+ | * <b>Isobase</b>, A Database of Functionally Related Orthologs, which incorporates PPI network data in addition to sequence similarity.<br />[http://cb.csail.mit.edu/cb/mna/isobase/ MIT, Cambridge, MA, USA] | ||
+ | * <b>MARRVEL</b>, Model organism Aggregated Resources for Rare Variant ExpLoration:<br />[http://marrvel.org/ MARRVEL, Baylor College of Medecine, Houston, TX, USA] | ||
+ | * <b>OMA Browser</b>, The OMA (“Orthologous MAtrix”) project is a method and database for the inference of orthologs among complete genomes.<br />[https://omabrowser.org/oma/home/ Nucl. Acids Res. 43 (D1): D240-D249. doi: 10.1093/nar/gku1158] | ||
* <b>OrthoDB</b>, The Hierarchical Catalog of Orthologs<br />[https://www.orthodb.org/ Zdobnov Computational Evolutionary Genomics group, University of Geneva, Geneva, Switzerland] | * <b>OrthoDB</b>, The Hierarchical Catalog of Orthologs<br />[https://www.orthodb.org/ Zdobnov Computational Evolutionary Genomics group, University of Geneva, Geneva, Switzerland] | ||
− | * <b>OrthoMCL</b>, Ortholog Groups of Protein Sequences<br />[ | + | * <b>OrthoFinder</b>, A comprehensive platform for comparative genomics that finds orthogroups and orthologs, infers rooted gene trees for all orthogroups and identifies all of the gene duplication events.<br />[http://www.stevekellylab.com/software/orthofinder University of Oxford, Oxford, UK] |
+ | * <b>OrthoInspector</b>, A software suite for inference of orthologous relationships between protein coding-genes and an online resource to access and query precomputed orthology databases.<br />[https://lbgi.fr/orthoinspector/ ICube Laboratory, Graffenstaden, France] | ||
+ | * <b>OrthoMCL</b>, Ortholog Groups of Protein Sequences<br />[https://orthomcl.org/orthomcl/ EuPathDB] | ||
* <b>PANTHER</b>, PANTHER Classification System<br />[http://pantherdb.org/genes/ Thomas Lab, University of Southern California, Los Angeles, CA, USA] | * <b>PANTHER</b>, PANTHER Classification System<br />[http://pantherdb.org/genes/ Thomas Lab, University of Southern California, Los Angeles, CA, USA] | ||
* <b>PhylomeDB</b>, PhylomeDB is a public database for complete catalogs of gene phylogenies (phylomes)<br />[http://phylomedb.org/ Comparative Genomics Groups at CRB, Barcelona, Spain] | * <b>PhylomeDB</b>, PhylomeDB is a public database for complete catalogs of gene phylogenies (phylomes)<br />[http://phylomedb.org/ Comparative Genomics Groups at CRB, Barcelona, Spain] | ||
− | * <b>Roundup</b>, A large-scale orthology database using the Reciprocal Smallest Distance (RSD) algorithm.<br />[ | + | * <b>Roundup</b>, A large-scale orthology database using the Reciprocal Smallest Distance (RSD) algorithm.<br />[https://wall-lab.stanford.edu/projects/roundup/ Wall lab, Stanford University, Palo Alto, CA, USA] |
* <b>TreeFam</b>, A database composed of phylogenetic trees inferred from animal genomes. It provides orthology/parology predictions as well the evolutionary history of genes.<br />[http://www.treefam.org/ EMBL-EBI, Hinxton, UK] | * <b>TreeFam</b>, A database composed of phylogenetic trees inferred from animal genomes. It provides orthology/parology predictions as well the evolutionary history of genes.<br />[http://www.treefam.org/ EMBL-EBI, Hinxton, UK] | ||
=Phylogenetic Comparison Tools= | =Phylogenetic Comparison Tools= | ||
− | * <b>DIOPT</b>, DRSC Integrative Ortholog Prediction Tool:<br />[ | + | * <b>DIOPT</b>, DRSC Integrative Ortholog Prediction Tool:<br />[https://www.flyrnai.org/cgi-bin/DRSC_orthologs.pl DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | + | * <b>HomoloGene</b> -- automated system fo constructing putative homology groups:<br />[https://www.ncbi.nlm.nih.gov/datasets/gene/ NCBI, Bethesda, MA, USA] | |
− | * <b>HomoloGene</b> -- automated system fo constructing putative homology groups:<br />[ | + | * <b>InParanoiDB 9</b>, Ortholog groups with inparalogs for proteins and protein domains<br />[https://inparanoidb.sbc.su.se/about/ InParanoiDB 9, Stockholm Bioinformatics Centre, Stockholm, Sweden] |
− | * <b> | + | * <b>MANTiS</b>, a phylogenetic framework for multi-species genome comparisons:<br />[https://www.mantisdb.org/MANTiS/Welcome.html MANTiS, Université Libre de Bruxelles, Belgium] |
− | * <b>MANTiS</b>, a phylogenetic framework for multi-species genome comparisons:<br />[ | ||
* <b>OrthoDB</b>, the Hierarchical Catalog of Orthologs:<br />[http://www.orthodb.org/ OrthoDB, University of Geneva, Switzerland] | * <b>OrthoDB</b>, the Hierarchical Catalog of Orthologs:<br />[http://www.orthodb.org/ OrthoDB, University of Geneva, Switzerland] | ||
− | + | * <b>Vista Tools</b> -- Whole Genome Comparative Analysis of the ''D. melanogaster'' (CAF1) Genome:<br />[https://pipeline.lbl.gov/cgi-bin/gateway2?bg=droMel_caf1&selector=vistapoint Whole Genome Comparative Analysis of the D. melanogaster (CAF1) Genome, LBNL, USA] | |
− | * <b>Vista Tools</b> -- Whole Genome Comparative Analysis of the ''D. melanogaster'' (CAF1) Genome:<br />[ | ||
=Population Biology and Polymorphism Resources= | =Population Biology and Polymorphism Resources= | ||
− | * <b>BDGP Single Nucleotide Polymorphism (SNP) Project</b>:<br />[ | + | * <b>BDGP Single Nucleotide Polymorphism (SNP) Project</b>:<br />[https://www.fruitfly.org/SNP/ Berkeley Drosophila Genome Project, University of California, Berkeley, USA] |
* <b>Drosophila Genetics Reference Panel</b>:<br />[https://www.hgsc.bcm.edu/arthropods/drosophila-genetic-reference-panel DGRP, Baylor College of Medicine, Houston, TX and NC State University, Raleigh, NC USA] | * <b>Drosophila Genetics Reference Panel</b>:<br />[https://www.hgsc.bcm.edu/arthropods/drosophila-genetic-reference-panel DGRP, Baylor College of Medicine, Houston, TX and NC State University, Raleigh, NC USA] | ||
* <b>Drosophila Genetics Reference Panel 2</b>:<br />[http://dgrp2.gnets.ncsu.edu/ DGRP2, NC State University, Raleigh, NC and Baylor College of Medicine, Houston, TX, USA] | * <b>Drosophila Genetics Reference Panel 2</b>:<br />[http://dgrp2.gnets.ncsu.edu/ DGRP2, NC State University, Raleigh, NC and Baylor College of Medicine, Houston, TX, USA] | ||
* <b>Drosophila Genome Nexus</b>: a population genomic resource that provides ''D. melanogaster'' genomes from multiple sources<br />[http://www.johnpool.net/genomes.html University of Wisconsin, Madison, WI, USA] | * <b>Drosophila Genome Nexus</b>: a population genomic resource that provides ''D. melanogaster'' genomes from multiple sources<br />[http://www.johnpool.net/genomes.html University of Wisconsin, Madison, WI, USA] | ||
− | + | * <b>DSPR</b>, Drosophila Synthetic Population Resource:<br />[https://wfitch.bio.uci.edu/~dspr/index.html DSPR, University of Kansas and UC Irvine, USA] | |
− | + | * <b>Global Diversity Lines</b>: a Five Continent Reference Panel of Sequenced Drosophila melanogaster Strains<br />[https://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?study=SRP050151 Global Diversity Lines, Cornell University, Ithaca, NY, USA] | |
− | * <b>DSPR</b>, Drosophila Synthetic Population Resource:<br />[ | ||
− | |||
− | * <b>Global Diversity Lines</b>: a Five Continent Reference Panel of Sequenced Drosophila melanogaster Strains<br />[ | ||
− | =Protein Analysis= | + | =Protein Analysis and Modification= |
− | * <b>ExPASy</b>, the SIB Bioinformatics Resource Portal which provides access to scientific databases and software tools in different areas of life sciences:<br />[ | + | * <b>AlphaFold Protein Structure Database</b>, AlphaFold DB provides open access to protein structure predictions; An AI system developed by DeepMind that predicts a protein’s 3D structure from its amino acid sequence:<br />[https://alphafold.ebi.ac.uk/ EMBL-EBI, Wellcome Genome Campus, Hinxton, Cambridgeshire, UK] |
− | * <b>GPCRDB</b>, information system for G protein-coupled receptors (GPCRs):<br />[ | + | * <b>ExPASy</b>, the SIB Bioinformatics Resource Portal which provides access to scientific databases and software tools in different areas of life sciences:<br />[https://www.expasy.org/ SIB Swiss Institute of Bioinformatics, Genève, Switzerland] |
+ | * <b>GPCRDB</b>, information system for G protein-coupled receptors (GPCRs):<br />[https://gpcrdb.org/ GPCRDB, The GPCRDB (G Protein-Coupled Receptor Data Base) partnership] | ||
* <b>InterPro</b> protein domain analysis of Drosophila:<br />[http://www.ebi.ac.uk/interpro/ InterPro: protein sequence analysis & classification, EBI, UK] | * <b>InterPro</b> protein domain analysis of Drosophila:<br />[http://www.ebi.ac.uk/interpro/ InterPro: protein sequence analysis & classification, EBI, UK] | ||
− | * <b>MEROPS</b>, an information resource for peptidases:<br />[ | + | * <b>iProteinDB</b> Integrated Protein Database of Post Translational Modifications for Drosophila Genes:<br />[https://www.flyrnai.org/tools/iproteindb/web/ iProteinDB, Harvard Medical School, Boston, MA, USA] |
− | * <b>ModBase</b>, Database of Comparative Protein Structure Models:<br />[ | + | * <b>MEROPS</b>, an information resource for peptidases:<br />[https://www.ebi.ac.uk/merops/ MEROPS, Wellcome Trust Sanger Institute, Hinxton, UK] |
− | * <b>Panther (Protein Analysis THrough Evolutionary Relationships)</b> protein classification system:<br />[ | + | * <b>ModBase</b>, Database of Comparative Protein Structure Models:<br />[https://modbase.compbio.ucsf.edu/modbase-cgi/index.cgi ModBase, UCSF, San Francisco, CA, USA] |
− | * <b>PDB</b>, Protein Data Bank:<br />[ | + | * <b>Panther (Protein Analysis THrough Evolutionary Relationships)</b> protein classification system:<br />[https://www.pantherdb.org/ Panther Protein Classification System, Applied Biosystems & Celera, USA] |
− | * <b>PhospoPep</b>, | + | * <b>PDB</b>, Protein Data Bank:<br />[https://www.rcsb.org/ PDB, The Research Collaboratory for Structural Bioinformatics (RCSB) consortium] |
− | * <b>ProteinProspector</b>, a proteomics tools for mining sequence databases in conjunction with Mass Spectrometry experiments: <br />[ | + | * <b>PhospoPep</b>, Protein Phosphorylation Database: <br />[http://phosphopep.org/ Institute for Systems Biology, Seattle, Washington, USA] |
+ | * <b>ProteinProspector</b>, a proteomics tools for mining sequence databases in conjunction with Mass Spectrometry experiments: <br />[https://prospector.ucsf.edu/prospector/mshome.htm UCSF Mass Spectrometry Facility, San Francisco, California, USA] | ||
* <b>RBPDB</b>, the database of RNA-binding protein specificities:<br />[http://rbpdb.ccbr.utoronto.ca/ RBPDB] | * <b>RBPDB</b>, the database of RNA-binding protein specificities:<br />[http://rbpdb.ccbr.utoronto.ca/ RBPDB] | ||
* <b>SMART</b>, Simple Modular Architecture Research Tool:<br />[http://smart.embl-heidelberg.de/ SMART, Protein Domain Analysis, EMBL, Heidleberg] | * <b>SMART</b>, Simple Modular Architecture Research Tool:<br />[http://smart.embl-heidelberg.de/ SMART, Protein Domain Analysis, EMBL, Heidleberg] | ||
− | * <b>UniProtKB</b>, a comprehensive catalog of information on proteins:<br />[https://www.uniprot.org | + | * <b>UniProtKB</b>, a comprehensive catalog of information on proteins:<br />[https://www.uniprot.org/ UniProtKB, The UniProt Consortium] |
=Public Education= | =Public Education= | ||
Line 296: | Line 334: | ||
* <b>droso4schools</b>, An online resource for school lessons using the fruit fly Drosophila:<br />[https://droso4schools.wordpress.com/ Manchester Fly Facility, University of Manchester, UK] | * <b>droso4schools</b>, An online resource for school lessons using the fruit fly Drosophila:<br />[https://droso4schools.wordpress.com/ Manchester Fly Facility, University of Manchester, UK] | ||
* <b>''Drosophila'' & Computer Programming Game</b>, A scratch computer game based on the Drosophila life cycle: <br /> [https://poppi62.wordpress.com/2015/08/28/school-flies/#ICT Manchester Fly Facility, University of Manchester, UK] | * <b>''Drosophila'' & Computer Programming Game</b>, A scratch computer game based on the Drosophila life cycle: <br /> [https://poppi62.wordpress.com/2015/08/28/school-flies/#ICT Manchester Fly Facility, University of Manchester, UK] | ||
− | * <b> | + | * <b>Experiments with Drosophila for Biology Courses</b>, An e-resource book for laboratory experiments at under- and post-graduate levels and for research projects in Biology courses :<br />[https://www.ias.ac.in/Publications/e-Books/Experiments_with_Drosophila_for_Biology_Courses Indian Academy of Sciences, India] [http://ftp.flybase.org/flybase/associated_files/Experiments_with_Drosophila_for_Biology_Courses.pdf pdf here ] |
* <b>FlyMove</b>, an Image, Movie and Interactive Shockwave Resource:<br />[http://flymove.uni-muenster.de/ FlyMove, Muenster, Germany] | * <b>FlyMove</b>, an Image, Movie and Interactive Shockwave Resource:<br />[http://flymove.uni-muenster.de/ FlyMove, Muenster, Germany] | ||
* <b>Lay articles</b>, A collection of lay articles about fly research: <br />[https://droso4public.wordpress.com/lay-articles/ Manchester Fly Facility, University of Manchester, UK] | * <b>Lay articles</b>, A collection of lay articles about fly research: <br />[https://droso4public.wordpress.com/lay-articles/ Manchester Fly Facility, University of Manchester, UK] | ||
Line 302: | Line 340: | ||
=RNAi= | =RNAi= | ||
− | * <b>DRSC-TRiP-FGR</b>, DRSC/TRiP Functional Genomics Resources:<br />[ | + | * <b>DRSC-TRiP-FGR</b>, DRSC/TRiP Functional Genomics Resources:<br />[https://fgr.hms.harvard.edu/ DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>Search by gene(s) to find hit information for genes and DRSC amplicons in DRSC screens</b>:<br /> [ | + | * <b>Search by gene(s) to find hit information for genes and DRSC amplicons in DRSC screens</b>:<br /> [https://www.flyrnai.org/RNAi_find_hit_info.html DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>FlyRNAi blog</b>, a DRSC resource concerning RNAi, cell-based assays, <i>Drosophila</i> cell culture, high-throughput screening, & fly biology:<br />[http:// | + | * <b>FlyRNAi blog</b>, a DRSC resource concerning RNAi, cell-based assays, <i>Drosophila</i> cell culture, high-throughput screening, & fly biology:<br />[http://flyrnai.blogspot.com/ flyrnai.blogspot.com] |
− | * <b>Fosmid rescue analysis tool</b> for identification of fosmids appropriate for cross-species rescue of RNAi:<br />[ | + | * <b>Fosmid rescue analysis tool</b> for identification of fosmids appropriate for cross-species rescue of RNAi:<br />[https://www.flyrnai.org/cgi-bin/RNAi_find_rescue_compl.pl DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
* <b>GenomeRNAi</b>, Heidelberg, a database of RNA interference (RNAi) screens:<br />[http://www.genomernai.org GenomeRNAi, DKFZ, Heidelberg, Germany] | * <b>GenomeRNAi</b>, Heidelberg, a database of RNA interference (RNAi) screens:<br />[http://www.genomernai.org GenomeRNAi, DKFZ, Heidelberg, Germany] | ||
− | * <b>GESS</b>, off-target RNAi prediction:<br />[ | + | * <b>GESS</b>, off-target RNAi prediction:<br />[https://www.flyrnai.org/gess/ DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>Predicted Off-Target Free Sequence Regions</b>:<br />[ | + | * <b>Predicted Off-Target Free Sequence Regions</b>:<br />[https://www.flyrnai.org/RNAi_find_frag_free.html DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>RNAiCut</b>, Automated Detection of Significant Genes from Functional Genomic Screens:<br /> [ | + | * <b>RNAiCut</b>, Automated Detection of Significant Genes from Functional Genomic Screens:<br /> [https://cb.csail.mit.edu/cb/RNAiCut/ Berger Lab, MIT, Boston, MA, USA] |
− | * <b>RSVP</b>, RNAi Stock Validation & Phenotypes:<br />[ | + | * <b>RSVP</b>, RNAi Stock Validation & Phenotypes:<br />[https://fgr.hms.harvard.edu/rsvp DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>SnapDragon</b>, RNAi Design:<br />[ | + | * <b>SnapDragon</b>, RNAi Design:<br />[https://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>TRiP</b>, Transgenic RNAi Project: <br />[ | + | * <b>TRiP</b>, Transgenic RNAi Project: <br />[https://fgr.hms.harvard.edu/fly-in-vivo-rnai DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>Tsinghua Fly Center</b>, | + | * <b>Tsinghua Fly Center</b>, THFC RNAi Stock Collection for triggering RNAi in soma and germline: <br />[https://thfc.zzbd.org/en THFC, Tsinghua Fly Center, Beijing, China] |
− | * <b>UP-TORR Fly</b>, a tool for identifying updated targets of RNAi reagents: <br />[ | + | * <b>UP-TORR Fly</b>, a tool for identifying updated targets of RNAi reagents: <br />[https://www.flyrnai.org/up-torr/ Updated Targets of RNAi Reagents, DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA] |
− | * <b>VDRC</b>, Vienna Drosophila Resource Center: <br />[ | + | * <b>VDRC</b>, Vienna Drosophila Resource Center: <br />[https://shop.vbc.ac.at/vdrc_store/ Genome-wide transgenic Drosophila RNAi libraries, VDRC, Vienna, Austria] |
=Sequence Analysis= | =Sequence Analysis= | ||
* <b>DCPD</b> -- Drosophila Core Promoter Database, A list of 205 Drosophila melanogaster core promoters aligned by their empirically determined transcription start site: <br />[http://labs.biology.ucsd.edu/Kadonaga/DCPD.htm Kadonaga Lab, UCSD, San Diego, California, USA] | * <b>DCPD</b> -- Drosophila Core Promoter Database, A list of 205 Drosophila melanogaster core promoters aligned by their empirically determined transcription start site: <br />[http://labs.biology.ucsd.edu/Kadonaga/DCPD.htm Kadonaga Lab, UCSD, San Diego, California, USA] | ||
− | + | * <b>FlyPrimerBank</b>, a comprehensive qPCR primer database for Drosophila: <br />[https://www.flyrnai.org/FlyPrimerBank FlyPrimerBank, DRSC, Harvard Medical School, Boston, MA, USA] | |
− | * <b>FlyPrimerBank</b>, a comprehensive qPCR primer database for Drosophila: <br />[ | ||
* <b>FGENESH</b>, Annotation of animal genomes-genes, promoters, functional motifs, protein sub-cellular localization: <br />[http://www.softberry.com/berry.phtml?topic=case_study_animal&no_menu=on Annotation of genomes, Softberry, USA] | * <b>FGENESH</b>, Annotation of animal genomes-genes, promoters, functional motifs, protein sub-cellular localization: <br />[http://www.softberry.com/berry.phtml?topic=case_study_animal&no_menu=on Annotation of genomes, Softberry, USA] | ||
+ | * <b>Gemme</b>, A fast, scalable and simple method to predict mutational landscapes from natural sequences: <br />[http://www.lcqb.upmc.fr/GEMME/Home.html Gemme, Sorbonne University, Paris, France] | ||
* <b>GenePalette</b>, a tool for genome sequence visualization and navigation: <br />[http://www.genepalette.org/ GenePalette, University of California, San Diego, USA] | * <b>GenePalette</b>, a tool for genome sequence visualization and navigation: <br />[http://www.genepalette.org/ GenePalette, University of California, San Diego, USA] | ||
− | * <b>Genie Gene finder for Drosophila: </b>:<br />[ | + | * <b>Genie Gene finder for Drosophila: </b>:<br />[https://www.fruitfly.org/seq_tools/genie.html Genie, Berkeley Drosophila Genome Project, University of California, Berkeley, USA] |
− | * <b>HRMA</b> -- High Resolution Melt Analysis: <br /> [ | + | * <b>HRMA</b> -- High Resolution Melt Analysis: <br /> [https://fgr.hms.harvard.edu/hrma HRMA, DRSC, Harvard Medical School, Boston, MA, USA] |
* <b>MEME Suite</b> -- Motif-based Sequence Analysis Tools: <br />[http://meme-suite.org/ MEME, U. of Queensland, U. of Washington, USA, UCSD, SDSC, NBCR, CBRC, and National Center for Research Resources] | * <b>MEME Suite</b> -- Motif-based Sequence Analysis Tools: <br />[http://meme-suite.org/ MEME, U. of Queensland, U. of Washington, USA, UCSD, SDSC, NBCR, CBRC, and National Center for Research Resources] | ||
− | * <b>RepeatMasker</b>: <br />[ | + | * <b>RepeatMasker</b>: <br />[https://www.repeatmasker.org/ RepeatMasker Institute for Systems Biology, Seattle, WA, USA] |
− | * <b>Splice Site Prediction for Drosophila</b>:<br />[ | + | * <b>Splice Site Prediction for Drosophila</b>:<br />[https://www.fruitfly.org/seq_tools/splice.html Splice Site Prediction, Berkeley Drosophila Genome Project, University of California, Berkeley, USA] |
* <b>Web Apollo</b>, a collaborative genomic annotation editor: <br />[http://apollo.berkeleybop.org/ Web Apollo, LBL, Berkeley, USA] | * <b>Web Apollo</b>, a collaborative genomic annotation editor: <br />[http://apollo.berkeleybop.org/ Web Apollo, LBL, Berkeley, USA] | ||
=Single Cell RNA-seq= | =Single Cell RNA-seq= | ||
* <b>ASAP</b> Automated Single-cell Analysis Pipeline: <br />[https://asap.epfl.ch/ ASAP, EPFL, Lausanne, Switzerland, Swiss Institute of Bioinformatics, Lausanne, Switzerland] | * <b>ASAP</b> Automated Single-cell Analysis Pipeline: <br />[https://asap.epfl.ch/ ASAP, EPFL, Lausanne, Switzerland, Swiss Institute of Bioinformatics, Lausanne, Switzerland] | ||
+ | |||
* <b>cisTopic</b> Probabilistic modelling of cis-regulatory topics from single cell epigenomics data: <br />[https://github.com/aertslab/cisTopic/ cisTopic, VIB Center for Brain & Disease Research, Leuven, Belgium, Department of Human Genetics, KU Leuven, Leuven, Belgium] | * <b>cisTopic</b> Probabilistic modelling of cis-regulatory topics from single cell epigenomics data: <br />[https://github.com/aertslab/cisTopic/ cisTopic, VIB Center for Brain & Disease Research, Leuven, Belgium, Department of Human Genetics, KU Leuven, Leuven, Belgium] | ||
+ | |||
+ | * <b>DeepCMC</b> A Neural Networks (NN) based approach for identifying genes that contain spatial information: <br />[https://github.com/TJU-CMC-Org/SingleCell-DREAM DeepCMC, Thomas Jefferson University, Philadelphia, PA, USA] | ||
+ | |||
+ | * <b>Distmap</b> To spatially map single cell RNA sequencing data by using an existing reference database of in situs: <br />[https://github.com/rajewsky-lab/distmap Distmap, BIMSB and MDC, Berlin, Germany] | ||
+ | |||
* <b>DRscDB</b> DRSC scRNA-seq DataBase: <br />[https://www.flyrnai.org/tools/single_cell/ DRscDB, DRSC, Harvard Medical School, Boston, USA] | * <b>DRscDB</b> DRSC scRNA-seq DataBase: <br />[https://www.flyrnai.org/tools/single_cell/ DRscDB, DRSC, Harvard Medical School, Boston, USA] | ||
+ | |||
+ | * <b>DVEX</b> Drosophila Virtual Expression eXplorer: <br />[https://shiny.mdc-berlin.de/DVEX/ DVEX, BIMSB and MDC, Berlin, Germany] | ||
+ | |||
+ | * <b>Fly Cell Atlas</b> A consortium of Drosophila researchers interested in single-cell genomics, transcriptomics, and epigenomics: <br />[https://flycellatlas.org/ Fly Cell Atlas, Founders - Stein Aerts, Leuven, Belgium, Bart Deplancke, Lausanne, Switzerland, Robert Zinzen, Berlin, Germany] | ||
+ | |||
+ | * <b>Lasso.TopX</b> An approach using the Lasso and ranking statistics for identifying genes that contain spatial information: <br />[https://github.com/TJU-CMC-Org/SingleCell-DREAM/ Lasso.TopX, Thomas Jefferson University, Philadelphia, PA, USA] | ||
+ | |||
+ | * <b>novoSpaRc</b> Predicts locations of single cells in space by solely using single-cell RNA sequencing data.: <br />[https://github.com/rajewsky-lab/novosparc novoSpaRc, Harvard and Broad Institute, Cambridge, MA, USA, Max Delbrück Center, Berlin, Germany] | ||
+ | |||
* <b>SCEA</b> Single Cell Expression Atlas - Single Cell Gene Expression Across Species: <br />[https://www.ebi.ac.uk/gxa/sc SCEA, EMBL-EBI, Hinxton, UK, Sanger Institute, Wellcome Genome Campus, Hinxton, UK, Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK] | * <b>SCEA</b> Single Cell Expression Atlas - Single Cell Gene Expression Across Species: <br />[https://www.ebi.ac.uk/gxa/sc SCEA, EMBL-EBI, Hinxton, UK, Sanger Institute, Wellcome Genome Campus, Hinxton, UK, Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK] | ||
+ | |||
* <b>SCENIC</b> Single-Cell Regulatory Network Inference and Clustering <br />[https://github.com/aertslab/SCENIC SCENIC, Center for Brain & Disease Research, Leuven, Belgium, KU Leuven, Leuven, Belgium, University of Liege, Liege, Belgium, VIB Center for Cancer Biology, Leuven, Belgium] | * <b>SCENIC</b> Single-Cell Regulatory Network Inference and Clustering <br />[https://github.com/aertslab/SCENIC SCENIC, Center for Brain & Disease Research, Leuven, Belgium, KU Leuven, Leuven, Belgium, University of Liege, Liege, Belgium, VIB Center for Cancer Biology, Leuven, Belgium] | ||
+ | |||
* <b>SCope</b> Fast Visualization Tool for Large-Scale and High Dimensional Single-Cell Data <br />[https://github.com/aertslab/SCope SCope, VIB KU Leuven, Leuven, Belgium] | * <b>SCope</b> Fast Visualization Tool for Large-Scale and High Dimensional Single-Cell Data <br />[https://github.com/aertslab/SCope SCope, VIB KU Leuven, Leuven, Belgium] | ||
+ | |||
+ | * <b>Seurat</b> An R package designed for QC, analysis, and exploration of single-cell RNA-seq data.: <br />[https://satijalab.org/seurat/ Seurat, NYU, NY, NY, USA] | ||
+ | |||
* <b>VSN</b> A Repository of Pipelines for Single-Cell Data Analysis in Nextflow DSL2: <br />[https://github.com/vib-singlecell-nf/vsn-pipelines VSN-Pipelines] | * <b>VSN</b> A Repository of Pipelines for Single-Cell Data Analysis in Nextflow DSL2: <br />[https://github.com/vib-singlecell-nf/vsn-pipelines VSN-Pipelines] | ||
=Taxonomy= | =Taxonomy= | ||
− | |||
* <b>FlyPhenomics</b>, phenotypic differences between species of the ''D. melanogaster'' subgroup:<br />[http://www.normalesup.org/~vorgogoz/FlyPhenomics/ FlyPhenomics, Paris, France] | * <b>FlyPhenomics</b>, phenotypic differences between species of the ''D. melanogaster'' subgroup:<br />[http://www.normalesup.org/~vorgogoz/FlyPhenomics/ FlyPhenomics, Paris, France] | ||
* <b>TaxoDros</b> Drosophilidae Taxonomic Database:<br />[https://bioinfo.museum.hokudai.ac.jp/ Drosophilidae Taxonomic Database, Japan] | * <b>TaxoDros</b> Drosophilidae Taxonomic Database:<br />[https://bioinfo.museum.hokudai.ac.jp/ Drosophilidae Taxonomic Database, Japan] | ||
* <b>TaxoDros</b>, the database on Taxonomy of Drosophilidae:<br />[http://www.taxodros.uzh.ch/ TaxoDros, University of Zurich, Switzerland] | * <b>TaxoDros</b>, the database on Taxonomy of Drosophilidae:<br />[http://www.taxodros.uzh.ch/ TaxoDros, University of Zurich, Switzerland] | ||
− | * <b>Taxonomy</b>, NCBI Taxonomy Database:<br />[ | + | * <b>Taxonomy</b>, NCBI Taxonomy Database:<br />[https://www.ncbi.nlm.nih.gov/taxonomy NCBI, Bethesda, MD USA] |
+ | * <b>Taxonomy of Drosophilidae - JDD - Japan Drosophila Database</b> <br />[http://www.drosophila.jp/jdd/index_en.html Japan Drosophila Database (JDD) on Taxonomy, Japan] | ||
=Transcription Regulation Databases and Tools= | =Transcription Regulation Databases and Tools= | ||
− | |||
* <b>CIS-BP</b>, The online library of transcription factors and their DNA binding motifs:<br />[http://cisbp.ccbr.utoronto.ca/ CIS-BP, Hughes Lab, University of Toronto, Toronto, Canada and Weirauch lab, Cincinnati Children's Hospital, Cincinnati, OH, USA] | * <b>CIS-BP</b>, The online library of transcription factors and their DNA binding motifs:<br />[http://cisbp.ccbr.utoronto.ca/ CIS-BP, Hughes Lab, University of Toronto, Toronto, Canada and Weirauch lab, Cincinnati Children's Hospital, Cincinnati, OH, USA] | ||
− | + | * <b>EPD</b>, the Eukaryotic Promoter Database:<br />[https://epd.expasy.org/epd EPD, Epalinges s/Lausanne, Switzerland] | |
− | |||
− | |||
− | * <b>EPD</b>, the Eukaryotic Promoter Database:<br />[ | ||
* <b>Fly Factor Survey</b> - Database of Drosophila TF DNA-binding Specificities: <br />[http://pgfe.umassmed.edu/ffs/ FLy Factor Survey, UMass Medical School, Worcester, MA, USA] | * <b>Fly Factor Survey</b> - Database of Drosophila TF DNA-binding Specificities: <br />[http://pgfe.umassmed.edu/ffs/ FLy Factor Survey, UMass Medical School, Worcester, MA, USA] | ||
− | * <b>FlyReg</b>, DNase I Footprint Database | + | * <b>FlyReg</b>, DNase I Footprint Database - retired<br />[http://www.flyreg.org/ University of Manchester Bioinformatics Resources, University of Manchester, UK] |
− | * <b>JASPAR</b>, A database of transcription factor (TF) binding profiles stored as position frequency matrices and TF flexible models for TFs across multiple species.<br />[ | + | * <b>JASPAR</b>, A database of transcription factor (TF) binding profiles stored as position frequency matrices and TF flexible models for TFs across multiple species.<br />[https://jaspar.elixir.no/ JASPAR] |
− | + | * <b>Neural Network Promoter Prediction for Drosophila</b>:<br />[https://www.fruitfly.org/seq_tools/promoter.html Neural Network Promoter Prediction, Berkeley Drosophila Genome Project, University of California, Berkeley, CA, USA] | |
− | * <b>Neural Network Promoter Prediction for Drosophila</b>:<br />[ | ||
* <b>OnTheFly</b>, Database of Drosophila Transcription Factors and Transcription Factor Binding Sites <br />[https://bhapp.c2b2.columbia.edu/OnTheFly/index.php OnTheFly, Columbia University, NY, USA] | * <b>OnTheFly</b>, Database of Drosophila Transcription Factors and Transcription Factor Binding Sites <br />[https://bhapp.c2b2.columbia.edu/OnTheFly/index.php OnTheFly, Columbia University, NY, USA] | ||
* <b>REDfly</b>, Regulatory Element Database for Drosophila:<br />[http://redfly.ccr.buffalo.edu/ REDfly, University at Buffalo, New York, Buffalo, NY, USA] | * <b>REDfly</b>, Regulatory Element Database for Drosophila:<br />[http://redfly.ccr.buffalo.edu/ REDfly, University at Buffalo, New York, Buffalo, NY, USA] | ||
+ | * <b>ReMap</b>, a large-scale integrative analysis of DNA-binding experiments for Homo sapiens, Mus musculus, Dropshiphila melanogaster, and Arabadopsis thaliana transcriptional regulators based on manual curation of ChIP-seq, ChIP-exo, DAP-seq from public sources:<br />[https://remap.univ-amu.fr/ ReMap, TAGC Inserm, Marseille, France] | ||
* <b>FlyTF.org</b>, The Drosophila Transcription Factor Database:<br />[https://www.mrc-lmb.cam.ac.uk/genomes/FlyTF/old_index.html FlyTF.org, MRC Cambridge, UK] | * <b>FlyTF.org</b>, The Drosophila Transcription Factor Database:<br />[https://www.mrc-lmb.cam.ac.uk/genomes/FlyTF/old_index.html FlyTF.org, MRC Cambridge, UK] | ||
− | * <b>TRANSFAC</b>, A database of transcription factors and their binding sites:<br />[http:// | + | * <b>scEnhancer</b>, A database of single cell enhancer annotation in human, mouse, and fly:<br />[http://enhanceratlas.net/scenhancer/ scEnhancer, Sun Yat-sen University, Shenzhen, China and Johns Hopkins University, Baltimore, MD, USA] |
+ | * <b>TRANSFAC</b>, A database of transcription factors and their binding sites:<br />[http://gene-regulation.com/pub/databases.html#transfac TRANSFAC, Braunschweig, Germany] | ||
* <b>UniPROBE</b>, Universal PBM (protein binding microarray) Resource for Oligonucleotide Binding Evaluation:<br />[http://thebrain.bwh.harvard.edu/uniprobe/ UniPROBE, Bulyk lab, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA] | * <b>UniPROBE</b>, Universal PBM (protein binding microarray) Resource for Oligonucleotide Binding Evaluation:<br />[http://thebrain.bwh.harvard.edu/uniprobe/ UniPROBE, Bulyk lab, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA] | ||
Latest revision as of 13:33, 19 March 2024
Atlases, Images and Videos
- Atlas of Drosophila Development, An Atlas following the main events of embryogenesis and post-embryonic development:
Volker Hartenstein and Interactive Fly, Bethesda, MD, USA - FlyBase Maps, A collection of illustrated and electron micrograph maps of polytene chromosomes for the 12 originally sequenced Drosophila species:
FlyBase Maps - FlyMove, an Image, Movie and Interactive Shockwave Resource:
FlyMove, Muenster, Germany - FlyPNS, D. melanogaster embryonic and larval peripheral nervous system:
FlyPNS, UMPC, Paris, France and Columbia University, NY, USA - FlyView, a Drosophila Image Database:
FlyView, Muenster, Germany - GETDB, a Gal4 enhancer trap database:
GETDB, Kyoto Institute of Technology, Kyoto, Japan - Jove, videos of experimental techniques:
Jove, Journal of Visualized Experiments, USA - MidgutAtlas, Gene expression in five pH-defined regions of the larval midgut of D. melanogaster:
Dow laboratory, University of Glascow, Scotland, UK - PeptideAtlas, a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments:
Seattle Proteome Center, Seattle, Washington, USA - Virtual Fly Brain - A 3D brain browser with point and click searches for neurons, transgenes and phenotypes:
Virtual Fly Brain, Edinburgh and Cambridge, UK
Cell Lines and Cell Culture
- Cellosaurus: A thesaurus of cell lines which attempts to list all cell lines used in biomedical research.
Swiss Institute of Bioinformatics, Geneva, Switzerland - DGRC: Cell Line Catalog:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC: Cell Culture Protocols:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC: Cell Culture FAQ Page:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC: Feeder Cells:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC: Fly Extract:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DRSC: General information about cell lines:
DRSC, Harvard Medical School, Boston, MA, USA - DSRC: Resource information about CRISPR modified cell lines:
DRSC, Harvard Medical School, Boston, MA, USA - DRSC: Protocol information about CRISPR modification of cell lines:
DRSC, Harvard Medical School, Boston, MA, USA
CRISPRs and TALENs
- Addgene sgRNA Libraries: Pooled sgRNA libraries for pooled CRISPR cell screening
Addgene, Watertown, MA, USA - CCTop CRISPR/Cas9 optimum target finder:
Center for Organismal Studies, Heidelberg University, Heidelberg, DE - CHOPCHOP CRISPR/Cas9 target online predictor, mammalian and other major model organisms including fly:
University of Bergen, Bergen, Norway - CRISPR-ERA A Fast and Comprehensive Guide RNA Design Tool for Genome Editing, Repression and Activation:
Lei Stanley Qi Lab and Xiaowo Wang Lab, Stanford University, Palo Alto, CA, USA - CRISPR fly design -- reagents, protocols, and results from fly CRISPR/Cas experiments:
Bullolk Lab, MRC Laboratory of Molecular Biology, Cambridge, UK - CRISPResso2 CRISPR Design Tool:
Broad Institute, Cambridge, MA, Harvard Medical School, Boston, MA, Massachusetts General Hospital, Boston, MA, Dana Farber Cancer Center, Boston, MA, Children's Hospital, Boston, MA - CRISPRscan Novel scoring algorithm for selecting sgRNAs
Giraldez Lab, Yale University, New Haven, CT, USA - DGRC Cell lines and vector reagents for CRISPR:
Drosophila Genomics Resource Center (DGRC), Indiana University, Bloomington, IN, USA - DGRC sgRNA libraries:
Drosophila Genomics Resource Center (DGRC), Indiana University, Bloomington, IN, USA - DRSC CRISPR Efficiency Tool for Assessment of Designs:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - DRSC Find CRISPRs, Drosophila CRISPR gRNA design search tool:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - TRiP CRISPR fly stock information:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - DRSC cell-based CRISPR modification:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - E-CRISP, Design of CRISPR Constructs:
DKFZ/Boutros lab, Heildelberg, Germany - E-TALEN, Design of TALEN Constructs:
DKFZ/Boutros lab, Heildelberg, Germany - flyCRISPR, Overview:
O'Connor-Giles, Wildonger, and Harrison Labs, University of Wisconsin-Madison, WI, USA - flyCRISPR, Target Finder:
O'Connor-Giles Lab, University of Wisconsin-Madison, WI, USA - Genome Engineering by CRISPR/Cas9 in Drosophila:
NIG/FLY/Ueda lab, Mishima, Japan - Mojo Hand, A TALEN Design Tool:
Ekker lab, Mayo Clinic, Rochester, MN, USA - TALengineering.org, A Comprehensive Resource for Engineered TAL Effector Technology:
Ekker lab, Joung Lab, MGH, Boston, USA - TRiP: Overview of sgRNA vectors:
TRiP, Harvard Medical School, Boston, MA, USA - Zhang Lab CRISPR Plasmids Available from Addgene CRISPR Plasmids:
Zhang Lab, MIT, Boston, MA, USA
Data Repositories
- Array Express - Functional Genomics Data:
Array Express, EMBL-EBI, Hinxton, UK - Berkeley Drosophila Genome Project (BDGP):
BDGP, University of California, Berkeley, USA - DDBJ, the DNA Data Bank of Japan:
DDBJ, National Institute of Genetics, Mishima, Japan - EMBL-EBI, The European Bioinformatics Institute:
EMBL-EBI, Hinxton, UK - ENA, European Nucleotide Archive:
ENA, European Nucleotide Archive, EMBL-EBI, Hinxton, UK - ENCODE, Encyclopedia of DNA Elements (including modENCODE and modERN):
ENCODE, Stanford University, Stanford, USA - Gene Disruption Project (GDP):
GDP, Baylor College of Medicine, Texas
Schematic drawings of Transposons - GenBank,the NIH genetic sequence database:
GenBank, NCBI, Bethesda, USA - GEO Datasets, Gene Expression Omnibus:
NCBI, Bethesda, USA - modERN, model organism Encyclopedia of Regulatory Networks:
modERN, University of Washington, Seattle, USA - NCBI, National Center for Biotechnology Information:
Bethesda, MD USA - NCBI Genome Data Viewer, A genome view of Drosophila melanogaster:
NCBI, Bethesda, USA - PubMed, Biomedical literature full-text archive:
PubMed, NCBI, Bethesda, USA - PubMed Central, Biomedical literature citations and abstracts:
PubMed Central, NCBI, Bethesda, USA - SRA, Sequence Read Archive:
SRA, NCBI, Bethesda, MD, USA - UCSC Genome Browser Gateway - D. melanogaster:
UCSC Genome Browser, UC Santa Cruz, Santa Cruz, USA
DNA Reagents
- Addgene Highly requested Drosophila plasmids:
Addgene, Watertown, MA, USA - Addgene sgRNA Libraries: Pooled sgRNA libraries for pooled CRISPR cell screening
Addgene, Watertown, MA, USA - DNASU Drosophila plasmids:
DNASU Plasmid Repository, Arizona State University, Tempe, AZ, USA - DGRC DNA Clones Catalog:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC DNA Clone Collections:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC DNA Clone FAQ Page:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC DNA Clone Protocols:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC DNA Vectors Catalog:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC DNA Vectors FAQs Page:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - DGRC DNA Vectors Protocols:
Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA - TRiP: VALIUM and WALIUM plasmid vector sets:
TRiP, Harvard Medical School, Boston, MA, USA - TRiP: Plasmid cloning protocols:
TRiP, Harvard Medical School, Boston, MA, USA - TRiP: Overview of sgRNA vectors:
TRiP, Harvard Medical School, Boston, MA, USA
Drosophila Genomes Data and Metadata
- DrosOmics, a comparative genomics browser to explore omics data in 52 natural populations of D. melanogaster:
Institute of Evolutionary Biology, CSIC, Universitat Pompeu Fabra, Barcelona, Spain - DroSpeGe, a view of Drosophila genome data, with genome maps and BLAST sequence search, for 12 species:
Genome Informatics Lab, Indiana University, Bloomington, Indiana, USA - List of Drosophila reference genome assemblies at FlyBase, NCBI and UCSC. File:DrosophilaGenomeAssemblies.xls (spreadsheet) Updated June 17, 2015
- D. albomicans Genome:
D. albomicans Organism Overview, NCBI, USA
D. albomicans Genome Assembly Report, NCBI, USA
D. albomicans Genome Assembly Report, ENA, UK - D. americana Genome:
D. americana Organism Overview, NCBI, USA
D. americana Genome Assembly Report, NCBI, USA
D. americana Genome Assembly Report, ENA, UK - D. ananassae Genome:
D. ananassae Organism Overview, NCBI, USA
D. ananassae Genome Assembly Report, NCBI, USA
D. ananassae Genome Assembly Report, ENA, UK - D. arizonae Genome:
D. arizonae Organism Overview, NCBI, USA
D. arizonae Genome Assembly and Annotation Report, NCBI, USA
D. arizonae Genome Assembly Report, ENA, UK - D. biarmipes Genome:
D. biarmipes Organism Overview, NCBI, USA
D. biarmipes Genome Assembly Report, NCBI, USA
D. biarmipes Genome Assembly Report, ENA, UK - D. bipectinata Genome:
D. bipectinata Organism Overview, NCBI, USA
D. bipectinata Genome Assembly Report, NCBI, USA
D. bipectinata Genome Assembly Report, ENA, UK - D. busckii Genome:
D. busckii Organism Overview, NCBI, USA
D. busckii Genome Assembly Report, NCBI, USA
D. busckii Genome Assembly Report, ENA, UK - D. erecta Genome:
D. erecta Organism Overview, NCBI, USA
D. erecta Genome Assembly Report, NCBI, USA
D. erecta Genome Assembly Report, ENA, UK - D. elegans Genome:
D. elegans Organism Overview, NCBI, USA
D. elegans Genome Assembly Report, NCBI, USA
D. elegans Genome Assembly Report, ENA, UK - D. eugracilis Genome:
D. eugracilis Organism Overview, NCBI, USA
D. eugracilis Genome Assembly Report, NCBI, USA
D. eugracilis Genome Assembly Report, ENA, UK - D. ficusphila Genome:
D. ficusphila Organism Overview, NCBI, USA
D. ficusphila Genome Assembly Report, NCBI, USA
D. ficusphila Genome Assembly Report, ENA, UK - D. grimshawi Genome:
D. grimshawi Organism Overview, NCBI, USA
D. grimshawi Genome Assembly Report, NCBI, USA
D. grimshawi Genome Assembly Report, ENA, UK - D. kikkawai Genome:
D. kikkawai Organism Overview, NCBI, USA
D. kikkawai Genome Assembly Report, NCBI, USA
D. kikkawai Genome Assembly Report, ENA, UK - D. melanogaster Genome:
D. melanogaster Organism Overview, NCBI, USA
D. melanogaster Genome Assembly Report, NCBI, USA
D. melanogaster Genome Assembly Report, ENA, UK - D. miranda Genome:
D. miranda Organism Overview, NCBI, USA
D. miranda Genome Assembly Report, NCBI, USA
D. miranda Genome Assembly Report, ENA, UK - D. mojavensis Genome:
D. mojavensis Organism Overview, NCBI, USA
D. mojavensis Genome Assembly Report, NCBI, USA
D. mojavensis Genome Assembly Report, ENA, UK - D. navojoa Genome:
D. navojoa Organism Overview, NCBI, USA
D. navojoa Genome Assembly Report, NCBI, USA
D. navojoa Genome Assembly Report, ENA, UK - D. persimilis Genome:
D. persimilis Organism Overview, NCBI, USA
D. persimilis Genome Assembly Report, NCBI, USA
D. persimilis Genome Assembly Report, ENA, UK - D. pseudoobscura Genome:
D. pseudoobscura Organism Overview, NCBI, USA
D. pseudoobscura Genome Assembly Report, NCBI, USA
D. pseudoobscura Genome Assembly Report, ENA, UK - D. rhopaloa Genome:
D. rhopaloa Organism Overview, NCBI, USA
D. rhopaloa Genome Assembly Report, NCBI, USA
D. rhopaloa Genome Assembly Report, ENA, UK - D. sechellia Genome:
D. sechellia Organism Overview, NCBI, USA
D. sechellia Genome Assembly Report, NCBI, USA
D. sechellia Genome Assembly Report, ENA, UK - D. simulans Genome:
D. simulans Organism Overview, NCBI, USA
D. simulans Genome Assembly Report, NCBI, USA
D. simulans Genome Assembly Report, ENA, UK - D. suzukii Genome:
D. suzukii Organism Overview, NCBI, USA
D. suzukii Genome Assembly Report, NCBI, USA
D. suzukii Genome Assembly Report, ENA, UK
D. suzukii SpottedWingFlyBase The Spotted Wing Drosophila Project, UC Davis and Oregon State University, USA - D. takahashii Genome:
D. takahashii Organism Overview, NCBI, USA
D. takahashii Genome Assembly Report, NCBI, USA
D. takahashii Genome Assembly Report, ENA, UK - D. virilis Genome:
D. virilis Organism Overview, NCBI, USA
D. virilis Genome Assembly Report, NCBI, USA
D. virilis Genome Assembly Report, ENA, UK - D. willistoni Genome:
D. willistoni Organism Overview, NCBI, USA
D. willistoni Genome Assembly Report, NCBI, USA
D. willistoni Genome Assembly Report, ENA, UK - D. yakuba Genome:
D. yakuba Organism Overview, NCBI, USA
D. yakuba Genome Assembly Report, NCBI, USA
D. yakuba Genome Assembly Report, ENA, UK]
Gene Expression Databases and Tools
- BDGP in situ, Patterns of gene expression in Drosophila embryogenesis:
Gene expression database, BDGP, University of California, Berkeley, USA - BGee Normal Gene Expression Data in Animals (based on RNA-Seq, Affymetrix, in situ hybridization, and EST data).
SIB, Swiss Institute of Bioinformatics, University of Lausanne, Lausanne, Switzerland - DIGITtally, Finding Genes of Interest DIGITtally:
DIGITtally, University of Glasgow, Glasgow, UK - FlyAtlas2, for exploring how genes are expressed in the tissues of D. melanogaster:
FlyAtlas2, University of Glasgow, Glasgow, UK - FlyExpress, an Expression Pattern Search Engine:
FlyExpress, Arizona State University, USA - Fly-FISH, A Database of Drosophila Embryo mRNA Localizaton Patterns:
Fly-FISH, University of Toronto, Toronto, Canada - FlyGut-seq, a transcriptomic resource that complements the original Flygut website:
Buchon Lab, Cornell University, Ithaca, NY, USA - FlyLight, Expression Patterns of GAL4, LexA, and Split-GAL4 Driver Lines to enable the visualization and precise manipulation of individual cell types in the Drosophila nervous system:
HHMI, Janelia Farms Research Campus - flytrap, a database of P{GAL4} enhancer traps and their expression in brains:
flytrap, University of Edinburgh, UK - Gene Expression Levels by Cell Line (based on modENCODE data):
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - GEO Profiles, Gene Expression Omnibus:
GEO, NCBI, Bethesda, MD USA - MidgutAtlas, Gene expression in five pH-defined regions of the larval midgut of D. melanogaster:
Dow laboratory, University of Glascow, Scotland, UK - RNAMiner, A Bioinformatics Protocol for Mining Large RNA-Seq Transcriptomics Data:
Cheng Lab, University of Missouri, Mizzou, Columbia, MO, USA
Gene Groups
- FlyBase Gene Groups, Manually curated reports on functionally related genes, based on the literature:
Gene group list, FlyBase - FlyBase Signaling Pathways, Manually curated reports on Signaling Pathways, based on the literature:
Pathways list, FlyBase - GLAD, an Online Database of Gene List Annotations for Drosophila:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - Interactive fly, Drosophila genes listed by biochemical function:
Interactive Fly, Thomas Brody and Society for Developmental Biology, MD, USA - Interactive fly, Maternally transcribed genes:
Interactive Fly, Thomas Brody and Society for Developmental Biology, MD, USA - Interactive fly, Zygotically transcribed genes:
Interactive Fly, Thomas Brody and Society for Developmental Biology, MD, USA - KEGG BRITE, Functional hierarchies and binary relationships of biological entities:
KEGG, Kyoto University, Kyoto, Japan and University of Tokyo, Tokyo, Japan - UCSC D. melanogaster Gene Sorter, displays tables of genes related by a variety of characteristics including protein-level homology, similarity of gene expression profiles, and genomic proximity.:
UCSC Genome Informatics Group, University of California, Santa Cruz, Santa Cruz, CA, USA
General Bioinformatics Tools
- Addgene, Early Career Researcher Toolbox: Free Online Molecular Biology Tools:
Addgene, Watertown, MA, USA - ApE, A plasmid Editor:
ApE, University of Utah, Salt Lake City, UT, USA - Bioconductor, Open Source Software for Bioinformatics:
Fred Hutchinson Cancer Research Center, Seattle, USA - Bioz, Insights and recommendations for products, equipment and assays:
Palo Alto, CA, USA - Cytoscape -- Network Data Integration, Analysis, and Visualization in a Box:
Cytoscape, Cytoscape Consortium, Canada, France, and USA - FlyMine, an Integrated Database for Drosophila and Anopheles Genomics:
University of Cambridge, United Kingdom - FlyNet, a network prioritization server for Drosophila melanogaster biology:
NetBioLab, Yonsei University, Seoul, Korea
Gene Set Enrichment Analysis
A summary table of selected GSEA tools with information about set up can be found here
- DAVID, Database for Annotation, Visualization and Integrated Discovery :
DAVID, Laboratory of Human Retrovirology and Immunoinformatics, Frederick, MD, USA] - Enrichr, Human and Mouse Gene Set Enrichment Analysis:
Enrichr, Center for Bioinfomatics, Mount Sinai, New York, NY, USA - MOET, Multi Ontology Enrichment Tool:
MOET, Rat Genome Database - modEnrichr, Model Organism Gene Set Enrichment Analysis:
modEnrichr, Center for Bioinfomatics, Mount Sinai, New York, NY, USA - flyEnrichr, D.melanogaster Gene Set Enrichment Analysis:
flyEnrichr, Center for Bioinfomatics, Mount Sinai, New York, NY, USA - GO term finder, Generic GO Term Finder:
GOTERMFINDER, Lewis-Sigler Institute for Integrative Genomics, Princeton University, New Jersey, NJ, USA - GOC, GO Enrichment Analysis:
Gene Ontology Consortium - GSEA, Gene Set Enrichment Analysis:
GSEA, Broad Institute, Cambridge, MA, USA - g:Profiler, g:Profiler/g:GOSt:
g:Profiler, Institute of Computer Science, University of Tartu, Tartu, Estonia - GOrilla, Gene Ontology enRIchment anaLysis and visuaLizAtion tool:
GOrilla, Technion, Israel Institute of Technology, Israel - PANGEA, PAthway, Network and Gene-set Enrichment Analysis:
PANGEA, DRSC, Harvard Medical School, Boston, MA, USA - PANTHER, PANTHER GO Enrichment Analysis:
PANTHER, University of Southern California, Los Angeles, CA, USA - REVIGO, Reduce + Visualize Gene Ontology:
REVIGO, Division of electronics, Ruđer Bošković Institute, Zagreb, Croatia - ShinyGO, ShinyGO 0.80:
ShinyGO, South Dakota State University, Brookings, SD, USA - WebGestalt, WebGestalt Gene Set Enrichment Analysis:
WebGestalt, Baylor College of Medicine, Houston, TX, USA
Genome Sequencing Projects
- D. pseudoobscura genome project:
D. pseudoobscura Human Genome Sequencing Center, Baylor College of Medicine, USA
Human Disease: Drosophila Models and Orthologous Genes
- AGR -- Alliance of Genome Resources:
Alliance of Genome Resources, RGD, MGD, ZFIN, FlyBase, WormBase, SGD, GO consortium, USA and UK - Bloomington Stock Center, Drosophila and Human Disease page:
Bloomington Stock Center, Bloomington, IN, USA - DIOPT-DIST, Disease-related Ortholog Tool:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - Drosophila as a Model for Human Diseases:
Interactive Fly, Bethesda, MD, USA - Drosophila Models of Human Disease, a blog by Stephanie Mohr and Annette Parks:
flydiseasemodels.blogspot.com/ - FlyNet Human Disease Prioritization:
NetBioLab, Yonsei University, Seoul, Korea - Gene2Function:
Gene2Function, Harvard Medical School, Boston, MA, USA - HuDis, high confidence human disease gene - fly gene ortholog pairs:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - MARRVEL, Model organism Aggregated Resources for Rare Variant ExpLoration:
MARRVEL, Baylor College of Medecine, Houston, TX, USA - MORPHIN, Model ORganism Projected on a Human Integrated gene Network:
MORPHIN, Lee Lab, Yonsei University, Korea and Marcotte Lab, University of Texas at Austin, USA.
Interaction and Pathway Databases
- BioCyc - FLY -- Curated Drosophila melanogaster Pathways:
FlyCyc, Harvard University, Cambridge, USA - BioGRID, The General Repository for Interaction Datasets:
BioGRID, Mount Sinai Hospital, Toronto, Canada - COMPLEAT, protein COMPLex Enrichment Analysis Tool:
COMPLEAT, DRSC, Harvard Medical School, Boston, MA, USA - DPiM, Drosophila Protein interaction Map:
DPiM, Harvard University Medical School, Boston, MA, USA - DroID, Drosophila Interactions Database:
Drosophila Interaction Database, Detroit, USA - FlyBase Signaling Pathways, Manually curated reports on Signaling Pathways, based on the literature:
Pathways list, FlyBase - FlyNet Gene Prioritization:
NetBioLab, Yonsei University, Seoul, Korea - FlyOde, FLY Organ Developmental Network:
FlyOde, Bogazici University, Istanbul, Turkey - HOMER, Software for motif discovery and next-gen sequencing analysis:
HOMER, University of California at San Diego, San Diego, CA, USA - IM Browser, Drosophila Interactions Database:
IM Browser, Wayne State University, Detroit, MI, USA - IntAct, Molecular Interaction Database:
IntAct, EMBL-EBI, Hinxton, UK - KEGG -- Kyoto Encyclopedia of Genes and Genomes:
KEGG, Kyoto, Japan - MIST -- Molecular Interactions Search Tool:
MIST, Harvard Medical School, Boston, MA, USA - Reactome Pathway Database:
Reactome, OICR, Ontario, Canada, EMBL-EBI, UK, NYU Medical Center, NY, USA - SignaLink, an integrated resource to analyze signaling pathway cross-talks, transcription factors, miRNAs and regulatory enzymes.:
SignaLink, Earlham Institute, Norwich, UK - STRING, Known and Predicted Protein-Protein Interactions
STRING, SIB, Switzerland CPR, Denmark, and EMBL, Heidelberg, Germany - WikiPathways, an open, public platform dedicated to the curation of biological pathways by and for the scientific community:
WikiPathways, Gladstone Institute, San Francisco, CA, USA and Maastricht University, The Netherlands
Metabolomics
- FlyMet, A Tissue- and Sex-Specific Metabolomic Atlas and Database :
FlyMet, University of Glasgow, Glasgow, Scotland
Miscellaneous
- AGR -- Alliance of Genome Resources:
Alliance of Genome Resources, RGD, MGD, ZFIN, FlyBase, WormBase, SGD, GO consortium, USA and UK - BioLitMine, Biological Literature Mining Tool for Human and Model Organisms:
BioLitMine, Harvard Medical School, Boston, MA, USA - Bionet Archives, a collection of bionet USENET newsgroups and parallel e-mail lists regarding Drosophila:
Genome Informatics Lab, Indiana University, Bloomington, Indiana, USA - Drosophila App, A mobile application developed by Genesee for use in fly research:
Android - Drosophila Information Service:
DIS, Norman, Oklahoma, USA - Drosophila Species Photographs, Photographs of 133 species of Drosophila:
Ehime University, Matsuyama, Japan - Drosophila Workers Unite! A laboratory manual for working with Drosophila:
Michele Markstein Lab, UMass Amherst, Amherst, MA, USA - The Encyclopedia of North American Drosophilids Volume 1: Drosophilids of the Midwest and Northeast:
by T. Werner, Michigan Technological University, Tessa Steenwinkel, Michigan Technological University, and J.Jaenike, University of Rochester - Fly Art - A compendium of arty imagery, sculptures or poetry inspired by Drosophila or flies in the wider sense.:
University of Manchester, Manchester, UK - Fly Labs and References, a large list of fly labs and recent publications:
Interactive Fly, Bethesda, MD, USA - FlyTree, the Drosophila Researcher Genealogy has been incorporated into the Academic Tree Project:
FlyTree, USA - Fully automated drosophila wing landmarking, Automatically finds more than 110 landmark points and creates a digital twin of the drosophila wing:
Datamarkin, Marseille, France - GenAge -- The Ageing Gene Database:
Human Ageing Genomic Resources, Institute of Integrative Biology, Liverpool, UK - Gene Lookup, Gene and Reagent Lookup:
Gene Lookup, DRSC, Harvard Medical School, Boston, MA, USA - GlyCosmos Portal, a Web portal aiming to integrate the glycosciences with the life sciences. It consists of Standards, Repositories and Data Resources, providing information about genes, proteins, lipids, pathways and diseases.:
GlyCosmos, GlyCosmos is a member of the GlySpace Alliance, consisting of ExPASy Glycomics, GlyCosmos, and GlyGen - Interactive Fly, A cyberspace guide to Drosophila development and metazoan evolution
Interactive Fly, Bethesda, MD, USA - Manchester Fly Facility, Resources - A compendium of information on Drosophila melanogaster as a model organism:
University of Manchester, Manchester, UK - Multiplex Fluorescent mRNA In Situ Hybridization, information on how to perform multiplex fluorescent mRNA in situ hybridization on Drosophila embryos:
Bier and McGinnis Labs, UCSD, San Diego, California, USA - What's hot today: Current papers in developmental biology and gene function:
Interactive Fly, Bethesda, MD, USA - The WWW Virtual Library--Drosophila:
Drosophila Virtual Library
Non-coding (ncRNA) Databases and Tools
General
- NONCODE, An integrated knowledge database dedicated to ncRNAs, especially lncRNAs:
NONCODE, Tsinghua University and Chinese Academy of Sciences, Beijing, China - Rfam, A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements :
Rfam, EMBL-EBI, Hinxton, UK - RNAcentral, Integrated access to a comprehensive and up-to-date set of non-coding RNA sequences provided by a collaborating group of Expert Databases :
RNAcentral, EMBL-EBI, Hinxton, UK
lncRNA
- LncRBase V.2, a database of information about lncRNAs from 8 species (human, mouse, fly, zebrafish, rat, chicken, C.elegans and cow) :
LncRBase, Bose Institute, West Bengal, India
miRNA
General
- miRBase, A searchable database of published miRNA sequences and annotations (current miBase release is 22 - last updated 2018):
miRBase, University of Manchester, UK
- NOTE - mirBase Release 21 (June 2014) uses old reference genome assemblies for D. melanogaster (Dmel_Release_5), D. simulans (Dsim_Release_1) and D. pseudoobscura (Dpse_Release_2). Linkouts from D. melanogaster miRNA genes to the Ensembl Genome browser (which displays Dmel_Release_6) will point to the old "Release_5" coordinates, which will be off the mark for many genes, especially those on X, 2R, 3R and 4 chromosomes.
- miRNEST, An integrated collection of miRNA predictions from high-throughput sequencing experiments and external database annotations (last updated 2015):
miRNEST, The Adam Mickiewicz University in Poznan, Poznan, Poland - TransmiR, A database of transcription factor-microRNA regulation (last updated 2018):
TransmiR, Peking University Health Science Center, Peking, Chin
miRNA Target Prediction
- DIANA microT-CDS, MicroRNA Target Prediction:
DIANA Tools, DIANA LAB, Athens, Greece - MinoTar, Predict microRNA Targets in Coding Sequence (last updated 2010):
MinoTar, DRSC, Harvard Medical School, Boston, MA, USA - TargetScanFly, Prediction of microRNA targets based on miRNA seed region and target conservation (last updated 2012):
TargetScanFly, Whitehead Institute and MIT Dept. of Biology, Cambridge, MA, USA
Validated miRNA Targets
- DIANA TarBase, MicroRNA Target Database (last updated 2017):
DIANA TarBase, DIANA LAB, Athens, Greece - miRTex, A text-mining system that extracts miRNA-gene regulation relations and miRNA-target relations from literature (last updated 2017):
miRTex, University of Delaware, Newark, DE, USA
Comparative miRNA Analysis
- microRNAviewer, A global view of homologous miRNA genes in many species (last updated 2012):
microRNAviewer, Tel Aviv University, Tel Aviv, Israel
rRNA
- SILVA, High quality ribosomal RNA databases (last updated 2020):
SILVA, Max Planck Institute for Marine Microbiology and Jacobs University, Bremen, Germany
tRNA
- tRFdb, A relational database of Transfer RNA related Fragments:
tRFdb, Dutta lab, University of Virginia, Charlottesville, VA, USA
snoRNA
- snOPY, a snoRNA orthological gene database providing comprehensive information about snoRNAs, snoRNA gene loci and target RNAs.:
snOPY, University of Miyazaki, Japan
SRP RNA
- SRPDB, Signal Recognition Particle Database:
SRPDB, University of Texas Health Science Center, San Antonio, USA
Ontology Resources
- BioPortal, a biomedical ontologies repository and browser:
BioPortal, The National Center for Biomedical Ontology, USA - Disease Ontology (DO), ontology of disease terms:
Disease Ontology - Gene Ontology Consortium (GO)
Gene Ontology Consortium - GOrilla -- Gene Ontology enRIchment and anaLysis and viuaLizAtion tool:
GOrilla, Israel - OboEdit, browser for OBO ontologies:
OboEdit - The OBO Foundry, an ontology registry:
OboFoundry - Ontobee, an ontologies browser:
Ontobee, He Group, University of Michigan Medical School, Ann Arbor, Michigan, USA - OLS - Ontology Lookup Service, an ontologies browser:
Ontology Lookup Service, EMBL-EBI, Hinxton, UK - Protege, browser for OWL ontologies:
Protege, Stanford Center for Biomedical Informatics Research, Stanford University School of Medicine, USA - QuickGO, a browser for Gene Ontology terms and annotations:
QuickGO, EMBL-EBI, Hinxton, UK
Orthology Predictions
- Compara, Ensembl Compara provides cross-species resources and analyses, at both the sequence level and the gene level.
Ensembl, Hinxton, UK - DIOPT, DRSC Integrative Ortholog Prediction Tool
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - DIOPT-DIST: DIOPT Diseases and Traits, DRSC Disease Gene Query Tool:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - EggNOG, A database of orthologous groups and functional annotation:
Computational Biology Group, EMBL, Heidelberg, Germany - Gene2Function:
Gene2Function, Harvard Medical School, Boston, MA, USA - Hieranoid, An orthology inference method using a hierarchical approach. Hieranoid performs pairwise orthology analysis using InParanoid at each node in a guide tree as it progresses from its leaves to the root.
Hieranoid, Stockholm Bioinformatics Centre, Stockholm, Sweden - HieranoiDB, An online database of orthologs inferred by Hieranoid 2 for a representative set of proteomes.
HieranoiDB, Stockholm Bioinformatics Centre, Stockholm, Sweden - Homologene, An automated system for constructing putative homology groups from the complete gene sets of a wide range of eukaryotic species.
NCBI, Bethesda, MD, USA - InParanoiDB 9, Ortholog groups with inparalogs for proteins and protein domains
InParanoiDB 9, Stockholm Bioinformatics Centre, Stockholm, Sweden - Isobase, A Database of Functionally Related Orthologs, which incorporates PPI network data in addition to sequence similarity.
MIT, Cambridge, MA, USA - MARRVEL, Model organism Aggregated Resources for Rare Variant ExpLoration:
MARRVEL, Baylor College of Medecine, Houston, TX, USA - OMA Browser, The OMA (“Orthologous MAtrix”) project is a method and database for the inference of orthologs among complete genomes.
Nucl. Acids Res. 43 (D1): D240-D249. doi: 10.1093/nar/gku1158 - OrthoDB, The Hierarchical Catalog of Orthologs
Zdobnov Computational Evolutionary Genomics group, University of Geneva, Geneva, Switzerland - OrthoFinder, A comprehensive platform for comparative genomics that finds orthogroups and orthologs, infers rooted gene trees for all orthogroups and identifies all of the gene duplication events.
University of Oxford, Oxford, UK - OrthoInspector, A software suite for inference of orthologous relationships between protein coding-genes and an online resource to access and query precomputed orthology databases.
ICube Laboratory, Graffenstaden, France - OrthoMCL, Ortholog Groups of Protein Sequences
EuPathDB - PANTHER, PANTHER Classification System
Thomas Lab, University of Southern California, Los Angeles, CA, USA - PhylomeDB, PhylomeDB is a public database for complete catalogs of gene phylogenies (phylomes)
Comparative Genomics Groups at CRB, Barcelona, Spain - Roundup, A large-scale orthology database using the Reciprocal Smallest Distance (RSD) algorithm.
Wall lab, Stanford University, Palo Alto, CA, USA - TreeFam, A database composed of phylogenetic trees inferred from animal genomes. It provides orthology/parology predictions as well the evolutionary history of genes.
EMBL-EBI, Hinxton, UK
Phylogenetic Comparison Tools
- DIOPT, DRSC Integrative Ortholog Prediction Tool:
DRSC/TRiP-FGR, Harvard Medical School, Boston, MA, USA - HomoloGene -- automated system fo constructing putative homology groups:
NCBI, Bethesda, MA, USA - InParanoiDB 9, Ortholog groups with inparalogs for proteins and protein domains
InParanoiDB 9, Stockholm Bioinformatics Centre, Stockholm, Sweden - MANTiS, a phylogenetic framework for multi-species genome comparisons:
MANTiS, Université Libre de Bruxelles, Belgium - OrthoDB, the Hierarchical Catalog of Orthologs:
OrthoDB, University of Geneva, Switzerland - Vista Tools -- Whole Genome Comparative Analysis of the D. melanogaster (CAF1) Genome:
Whole Genome Comparative Analysis of the D. melanogaster (CAF1) Genome, LBNL, USA
Population Biology and Polymorphism Resources
- BDGP Single Nucleotide Polymorphism (SNP) Project:
Berkeley Drosophila Genome Project, University of California, Berkeley, USA - Drosophila Genetics Reference Panel:
DGRP, Baylor College of Medicine, Houston, TX and NC State University, Raleigh, NC USA - Drosophila Genetics Reference Panel 2:
DGRP2, NC State University, Raleigh, NC and Baylor College of Medicine, Houston, TX, USA - Drosophila Genome Nexus: a population genomic resource that provides D. melanogaster genomes from multiple sources
University of Wisconsin, Madison, WI, USA - DSPR, Drosophila Synthetic Population Resource:
DSPR, University of Kansas and UC Irvine, USA - Global Diversity Lines: a Five Continent Reference Panel of Sequenced Drosophila melanogaster Strains
Global Diversity Lines, Cornell University, Ithaca, NY, USA
Protein Analysis and Modification
- AlphaFold Protein Structure Database, AlphaFold DB provides open access to protein structure predictions; An AI system developed by DeepMind that predicts a protein’s 3D structure from its amino acid sequence:
EMBL-EBI, Wellcome Genome Campus, Hinxton, Cambridgeshire, UK - ExPASy, the SIB Bioinformatics Resource Portal which provides access to scientific databases and software tools in different areas of life sciences:
SIB Swiss Institute of Bioinformatics, Genève, Switzerland - GPCRDB, information system for G protein-coupled receptors (GPCRs):
GPCRDB, The GPCRDB (G Protein-Coupled Receptor Data Base) partnership - InterPro protein domain analysis of Drosophila:
InterPro: protein sequence analysis & classification, EBI, UK - iProteinDB Integrated Protein Database of Post Translational Modifications for Drosophila Genes:
iProteinDB, Harvard Medical School, Boston, MA, USA - MEROPS, an information resource for peptidases:
MEROPS, Wellcome Trust Sanger Institute, Hinxton, UK - ModBase, Database of Comparative Protein Structure Models:
ModBase, UCSF, San Francisco, CA, USA - Panther (Protein Analysis THrough Evolutionary Relationships) protein classification system:
Panther Protein Classification System, Applied Biosystems & Celera, USA - PDB, Protein Data Bank:
PDB, The Research Collaboratory for Structural Bioinformatics (RCSB) consortium - PhospoPep, Protein Phosphorylation Database:
Institute for Systems Biology, Seattle, Washington, USA - ProteinProspector, a proteomics tools for mining sequence databases in conjunction with Mass Spectrometry experiments:
UCSF Mass Spectrometry Facility, San Francisco, California, USA - RBPDB, the database of RNA-binding protein specificities:
RBPDB - SMART, Simple Modular Architecture Research Tool:
SMART, Protein Domain Analysis, EMBL, Heidleberg - UniProtKB, a comprehensive catalog of information on proteins:
UniProtKB, The UniProt Consortium
Public Education
- About the history of Drosophila research, An extensive collection of media pertaining to the history of fruit fly research:
Manchester Fly Facility, University of Manchester, UK - droso4schools, An online resource for school lessons using the fruit fly Drosophila:
Manchester Fly Facility, University of Manchester, UK - Drosophila & Computer Programming Game, A scratch computer game based on the Drosophila life cycle:
Manchester Fly Facility, University of Manchester, UK - Experiments with Drosophila for Biology Courses, An e-resource book for laboratory experiments at under- and post-graduate levels and for research projects in Biology courses :
Indian Academy of Sciences, India pdf here - FlyMove, an Image, Movie and Interactive Shockwave Resource:
FlyMove, Muenster, Germany - Lay articles, A collection of lay articles about fly research:
Manchester Fly Facility, University of Manchester, UK - Manchester Fly Facility Public Resource Page:
Manchester Fly Facility, University of Manchester, UK
RNAi
- DRSC-TRiP-FGR, DRSC/TRiP Functional Genomics Resources:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - Search by gene(s) to find hit information for genes and DRSC amplicons in DRSC screens:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - FlyRNAi blog, a DRSC resource concerning RNAi, cell-based assays, Drosophila cell culture, high-throughput screening, & fly biology:
flyrnai.blogspot.com - Fosmid rescue analysis tool for identification of fosmids appropriate for cross-species rescue of RNAi:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - GenomeRNAi, Heidelberg, a database of RNA interference (RNAi) screens:
GenomeRNAi, DKFZ, Heidelberg, Germany - GESS, off-target RNAi prediction:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - Predicted Off-Target Free Sequence Regions:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - RNAiCut, Automated Detection of Significant Genes from Functional Genomic Screens:
Berger Lab, MIT, Boston, MA, USA - RSVP, RNAi Stock Validation & Phenotypes:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - SnapDragon, RNAi Design:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - TRiP, Transgenic RNAi Project:
DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - Tsinghua Fly Center, THFC RNAi Stock Collection for triggering RNAi in soma and germline:
THFC, Tsinghua Fly Center, Beijing, China - UP-TORR Fly, a tool for identifying updated targets of RNAi reagents:
Updated Targets of RNAi Reagents, DRSC-TRiP-FGR, Harvard Medical School, Boston, MA, USA - VDRC, Vienna Drosophila Resource Center:
Genome-wide transgenic Drosophila RNAi libraries, VDRC, Vienna, Austria
Sequence Analysis
- DCPD -- Drosophila Core Promoter Database, A list of 205 Drosophila melanogaster core promoters aligned by their empirically determined transcription start site:
Kadonaga Lab, UCSD, San Diego, California, USA - FlyPrimerBank, a comprehensive qPCR primer database for Drosophila:
FlyPrimerBank, DRSC, Harvard Medical School, Boston, MA, USA - FGENESH, Annotation of animal genomes-genes, promoters, functional motifs, protein sub-cellular localization:
Annotation of genomes, Softberry, USA - Gemme, A fast, scalable and simple method to predict mutational landscapes from natural sequences:
Gemme, Sorbonne University, Paris, France - GenePalette, a tool for genome sequence visualization and navigation:
GenePalette, University of California, San Diego, USA - Genie Gene finder for Drosophila: :
Genie, Berkeley Drosophila Genome Project, University of California, Berkeley, USA - HRMA -- High Resolution Melt Analysis:
HRMA, DRSC, Harvard Medical School, Boston, MA, USA - MEME Suite -- Motif-based Sequence Analysis Tools:
MEME, U. of Queensland, U. of Washington, USA, UCSD, SDSC, NBCR, CBRC, and National Center for Research Resources - RepeatMasker:
RepeatMasker Institute for Systems Biology, Seattle, WA, USA - Splice Site Prediction for Drosophila:
Splice Site Prediction, Berkeley Drosophila Genome Project, University of California, Berkeley, USA - Web Apollo, a collaborative genomic annotation editor:
Web Apollo, LBL, Berkeley, USA
Single Cell RNA-seq
- ASAP Automated Single-cell Analysis Pipeline:
ASAP, EPFL, Lausanne, Switzerland, Swiss Institute of Bioinformatics, Lausanne, Switzerland
- cisTopic Probabilistic modelling of cis-regulatory topics from single cell epigenomics data:
cisTopic, VIB Center for Brain & Disease Research, Leuven, Belgium, Department of Human Genetics, KU Leuven, Leuven, Belgium
- DeepCMC A Neural Networks (NN) based approach for identifying genes that contain spatial information:
DeepCMC, Thomas Jefferson University, Philadelphia, PA, USA
- Distmap To spatially map single cell RNA sequencing data by using an existing reference database of in situs:
Distmap, BIMSB and MDC, Berlin, Germany
- DRscDB DRSC scRNA-seq DataBase:
DRscDB, DRSC, Harvard Medical School, Boston, USA
- DVEX Drosophila Virtual Expression eXplorer:
DVEX, BIMSB and MDC, Berlin, Germany
- Fly Cell Atlas A consortium of Drosophila researchers interested in single-cell genomics, transcriptomics, and epigenomics:
Fly Cell Atlas, Founders - Stein Aerts, Leuven, Belgium, Bart Deplancke, Lausanne, Switzerland, Robert Zinzen, Berlin, Germany
- Lasso.TopX An approach using the Lasso and ranking statistics for identifying genes that contain spatial information:
Lasso.TopX, Thomas Jefferson University, Philadelphia, PA, USA
- novoSpaRc Predicts locations of single cells in space by solely using single-cell RNA sequencing data.:
novoSpaRc, Harvard and Broad Institute, Cambridge, MA, USA, Max Delbrück Center, Berlin, Germany
- SCEA Single Cell Expression Atlas - Single Cell Gene Expression Across Species:
SCEA, EMBL-EBI, Hinxton, UK, Sanger Institute, Wellcome Genome Campus, Hinxton, UK, Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK
- SCENIC Single-Cell Regulatory Network Inference and Clustering
SCENIC, Center for Brain & Disease Research, Leuven, Belgium, KU Leuven, Leuven, Belgium, University of Liege, Liege, Belgium, VIB Center for Cancer Biology, Leuven, Belgium
- SCope Fast Visualization Tool for Large-Scale and High Dimensional Single-Cell Data
SCope, VIB KU Leuven, Leuven, Belgium
- Seurat An R package designed for QC, analysis, and exploration of single-cell RNA-seq data.:
Seurat, NYU, NY, NY, USA
- VSN A Repository of Pipelines for Single-Cell Data Analysis in Nextflow DSL2:
VSN-Pipelines
Taxonomy
- FlyPhenomics, phenotypic differences between species of the D. melanogaster subgroup:
FlyPhenomics, Paris, France - TaxoDros Drosophilidae Taxonomic Database:
Drosophilidae Taxonomic Database, Japan - TaxoDros, the database on Taxonomy of Drosophilidae:
TaxoDros, University of Zurich, Switzerland - Taxonomy, NCBI Taxonomy Database:
NCBI, Bethesda, MD USA - Taxonomy of Drosophilidae - JDD - Japan Drosophila Database
Japan Drosophila Database (JDD) on Taxonomy, Japan
Transcription Regulation Databases and Tools
- CIS-BP, The online library of transcription factors and their DNA binding motifs:
CIS-BP, Hughes Lab, University of Toronto, Toronto, Canada and Weirauch lab, Cincinnati Children's Hospital, Cincinnati, OH, USA - EPD, the Eukaryotic Promoter Database:
EPD, Epalinges s/Lausanne, Switzerland - Fly Factor Survey - Database of Drosophila TF DNA-binding Specificities:
FLy Factor Survey, UMass Medical School, Worcester, MA, USA - FlyReg, DNase I Footprint Database - retired
University of Manchester Bioinformatics Resources, University of Manchester, UK - JASPAR, A database of transcription factor (TF) binding profiles stored as position frequency matrices and TF flexible models for TFs across multiple species.
JASPAR - Neural Network Promoter Prediction for Drosophila:
Neural Network Promoter Prediction, Berkeley Drosophila Genome Project, University of California, Berkeley, CA, USA - OnTheFly, Database of Drosophila Transcription Factors and Transcription Factor Binding Sites
OnTheFly, Columbia University, NY, USA - REDfly, Regulatory Element Database for Drosophila:
REDfly, University at Buffalo, New York, Buffalo, NY, USA - ReMap, a large-scale integrative analysis of DNA-binding experiments for Homo sapiens, Mus musculus, Dropshiphila melanogaster, and Arabadopsis thaliana transcriptional regulators based on manual curation of ChIP-seq, ChIP-exo, DAP-seq from public sources:
ReMap, TAGC Inserm, Marseille, France - FlyTF.org, The Drosophila Transcription Factor Database:
FlyTF.org, MRC Cambridge, UK - scEnhancer, A database of single cell enhancer annotation in human, mouse, and fly:
scEnhancer, Sun Yat-sen University, Shenzhen, China and Johns Hopkins University, Baltimore, MD, USA - TRANSFAC, A database of transcription factors and their binding sites:
TRANSFAC, Braunschweig, Germany - UniPROBE, Universal PBM (protein binding microarray) Resource for Oligonucleotide Binding Evaluation:
UniPROBE, Bulyk lab, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA
Suggest a tool or resource
- Please contact Flybase to suggest a tool or resource for the list.