Difference between revisions of "FlyBase:Papers with technical advances"
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! ''FBrf number''!!''PubMed ID''!!''Journal''!!''Title''!!''Author description of technical advance'' | ! ''FBrf number''!!''PubMed ID''!!''Journal''!!''Title''!!''Author description of technical advance'' | ||
|- | |- | ||
− | | FBrf0247772 || [https://pubmed.ncbi.nlm.nih.gov/33443210/ 33443210] || Proc. Natl. Acad. Sci. U.S.A. || Precise genome engineering in Drosophila using prime editing.|| This paper describes reagents (e.g. transgenic flies, plasmids, cloning protocols) to perform prime editing in Drosophila melanogaster. | + | | [https://flybase.org/reports/FBrf0247772 FBrf0247772] || [https://pubmed.ncbi.nlm.nih.gov/33443210/ 33443210] || Proc. Natl. Acad. Sci. U.S.A. || Precise genome engineering in Drosophila using prime editing.|| This paper describes reagents (e.g. transgenic flies, plasmids, cloning protocols) to perform prime editing in Drosophila melanogaster. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247854 || [https://pubmed.ncbi.nlm.nih.gov/31712250/ 31712250] || J. Lipid Res. || Lipidome-wide 13C flux analysis: a novel tool to estimate the turnover of lipids in organisms and cultures.||Metabolic flux analysis of lipids | + | | [https://flybase.org/reports/FBrf0247854 FBrf0247854] || [https://pubmed.ncbi.nlm.nih.gov/31712250/ 31712250] || J. Lipid Res. || Lipidome-wide 13C flux analysis: a novel tool to estimate the turnover of lipids in organisms and cultures.||Metabolic flux analysis of lipids |
|- | |- | ||
− | | FBrf0247762 || [https://pubmed.ncbi.nlm.nih.gov/33149298/ 33149298] || Nature || Neuronal diversity and convergence in a visual system developmental atlas.||Developmental scRNA-seq atlas of the Drosophila optic lobe | + | | [https://flybase.org/reports/FBrf0247762 FBrf0247762] || [https://pubmed.ncbi.nlm.nih.gov/33149298/ 33149298] || Nature || Neuronal diversity and convergence in a visual system developmental atlas.||Developmental scRNA-seq atlas of the Drosophila optic lobe |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247932 || [https://pubmed.ncbi.nlm.nih.gov/33025430/ 33025430] || Environ. Sci. Pollut. Res. Int. || Genes regulating development and behavior exhibited altered expression in Drosophila melanogaster exposed to bisphenol A: use of real-time quantitative PCR (qRT-PCR) and droplet digital PCR (ddPCR) in genotoxicity study.||Application of digital droplet PCR in gene expression study | + | | [https://flybase.org/reports/FBrf0247932 FBrf0247932] || [https://pubmed.ncbi.nlm.nih.gov/33025430/ 33025430] || Environ. Sci. Pollut. Res. Int. || Genes regulating development and behavior exhibited altered expression in Drosophila melanogaster exposed to bisphenol A: use of real-time quantitative PCR (qRT-PCR) and droplet digital PCR (ddPCR) in genotoxicity study.||Application of digital droplet PCR in gene expression study |
|- | |- | ||
− | | FBrf0247834 || [https://pubmed.ncbi.nlm.nih.gov/33346099/ 33346099] || Gene || Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination.||The transgenic sexing strains of Drosophila suzukii based on the testis-specific fluorescent expression. | + | | [https://flybase.org/reports/FBrf0247834 FBrf0247834] || [https://pubmed.ncbi.nlm.nih.gov/33346099/ 33346099] || Gene || Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination.||The transgenic sexing strains of Drosophila suzukii based on the testis-specific fluorescent expression. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247838 || [https://pubmed.ncbi.nlm.nih.gov/33490883/ 33490883] || FASEB Bioadv || Preference and detrimental effects of high fat, sugar, and salt diet in wild-caught Drosophila simulans are reversed by flight exercise.||New model of Flight exercise. New model of the Western Diet | + | | [https://flybase.org/reports/FBrf0247838 FBrf0247838] || [https://pubmed.ncbi.nlm.nih.gov/33490883/ 33490883] || FASEB Bioadv || Preference and detrimental effects of high fat, sugar, and salt diet in wild-caught Drosophila simulans are reversed by flight exercise.||New model of Flight exercise. New model of the Western Diet |
|- | |- | ||
− | | FBrf0247869 || [https://pubmed.ncbi.nlm.nih.gov/33495334/ 33495334] || Proc. Natl. Acad. Sci. U.S.A. || Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid.||Identification of neutral lipid-based secreted structures called microcarriers in the lumen of the male accessory gland, which can be stained by neutral lipid dyes like LipidTox and Nile Red. | + | | [https://flybase.org/reports/FBrf0247869 FBrf0247869] || [https://pubmed.ncbi.nlm.nih.gov/33495334/ 33495334] || Proc. Natl. Acad. Sci. U.S.A. || Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid.||Identification of neutral lipid-based secreted structures called microcarriers in the lumen of the male accessory gland, which can be stained by neutral lipid dyes like LipidTox and Nile Red. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247944 || [https://pubmed.ncbi.nlm.nih.gov/33514834/ 33514834] || Commun. Biol. || The level of oncogenic Ras determines the malignant transformation of Lkb1 mutant tissue in vivo.||Long-term in vivo imaging of Drosophila giant larvae tumor migration and invasion using SiMView microscopy. | + | | [https://flybase.org/reports/FBrf0247944 FBrf0247944] || [https://pubmed.ncbi.nlm.nih.gov/33514834/ 33514834] || Commun. Biol. || The level of oncogenic Ras determines the malignant transformation of Lkb1 mutant tissue in vivo.||Long-term in vivo imaging of Drosophila giant larvae tumor migration and invasion using SiMView microscopy. |
|- | |- | ||
− | | FBrf0247996 || [https://pubmed.ncbi.nlm.nih.gov/33532738/ 33532738] || STAR Protoc || Analyzing muscle structure and function throughout the larval instars in live Drosophila.||Methods to examine the musculature of the Drosophila larva. These methods include analysis of the muscle sarcomeres, particularly in the same larva throughout the different larval instars. This work contains methods to muscle structural changes to muscle functional changes. | + | | [https://flybase.org/reports/FBrf0247996 FBrf0247996] || [https://pubmed.ncbi.nlm.nih.gov/33532738/ 33532738] || STAR Protoc || Analyzing muscle structure and function throughout the larval instars in live Drosophila.||Methods to examine the musculature of the Drosophila larva. These methods include analysis of the muscle sarcomeres, particularly in the same larva throughout the different larval instars. This work contains methods to muscle structural changes to muscle functional changes. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0248021 || [https://pubmed.ncbi.nlm.nih.gov/33537561/ 33537561] || Micropub Biol || Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.||We verified that Drosophila S2R+ adapted to grow in serum-free media is amenable to RNAi interference-mediated gene knockdown. | + | | [https://flybase.org/reports/FBrf0248021 FBrf0248021] || [https://pubmed.ncbi.nlm.nih.gov/33537561/ 33537561] || Micropub Biol || Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.||We verified that Drosophila S2R+ adapted to grow in serum-free media is amenable to RNAi interference-mediated gene knockdown. |
|- | |- | ||
− | | FBrf0248088 || [https://pubmed.ncbi.nlm.nih.gov/33563972/ 33563972] || Nat. Commun. || Dynamic sex chromosome expression in Drosophila male germ cells.||single cell genomics of testes | + | | [https://flybase.org/reports/FBrf0248088 FBrf0248088] || [https://pubmed.ncbi.nlm.nih.gov/33563972/ 33563972] || Nat. Commun. || Dynamic sex chromosome expression in Drosophila male germ cells.||single cell genomics of testes |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0248123 || [https://pubmed.ncbi.nlm.nih.gov/33558234/ 33558234] || Proc. Natl. Acad. Sci. U.S.A. || The Krüppel-like factor Cabut has cell cycle regulatory properties similar to E2F1.||new reporter line and new null allele were generated in this paper | + | | [https://flybase.org/reports/FBrf0248123 FBrf0248123] || [https://pubmed.ncbi.nlm.nih.gov/33558234/ 33558234] || Proc. Natl. Acad. Sci. U.S.A. || The Krüppel-like factor Cabut has cell cycle regulatory properties similar to E2F1.||new reporter line and new null allele were generated in this paper |
|- | |- | ||
− | | FBrf0247919 || [https://pubmed.ncbi.nlm.nih.gov/33239786/ 33239786] || Nature || Neural circuit mechanisms of sexual receptivity in Drosophila females.||split-GAL4 lines labeling specific cell types | + | | [https://flybase.org/reports/FBrf0247919 FBrf0247919] || [https://pubmed.ncbi.nlm.nih.gov/33239786/ 33239786] || Nature || Neural circuit mechanisms of sexual receptivity in Drosophila females.||split-GAL4 lines labeling specific cell types |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0248082 || [https://pubmed.ncbi.nlm.nih.gov/33495632/ 33495632] || Nat. Cell Biol. || Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition.||A translational fidelity reporter (UAS-Fluc-STOP-Nluc) - Drosophila mutant strains (RPS23-R67K, RPS26-KO) | + | | [https://flybase.org/reports/FBrf0248082 FBrf0248082] || [https://pubmed.ncbi.nlm.nih.gov/33495632/ 33495632] || Nat. Cell Biol. || Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition.||A translational fidelity reporter (UAS-Fluc-STOP-Nluc) - Drosophila mutant strains (RPS23-R67K, RPS26-KO) |
|- | |- | ||
− | | FBrf0248109 || [https://pubmed.ncbi.nlm.nih.gov/33558226/ 33558226] || Proc. Natl. Acad. Sci. U.S.A. || Multisensory interactions regulate feeding behavior in Drosophila.||The modified PER assay can deliver stimuli not only for taste, but also for other senses. we made a line that can express the hair plate in legs. | + | | [https://flybase.org/reports/FBrf0248109 FBrf0248109] || [https://pubmed.ncbi.nlm.nih.gov/33558226/ 33558226] || Proc. Natl. Acad. Sci. U.S.A. || Multisensory interactions regulate feeding behavior in Drosophila.||The modified PER assay can deliver stimuli not only for taste, but also for other senses. we made a line that can express the hair plate in legs. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247921 || [https://pubmed.ncbi.nlm.nih.gov/33242000/ 33242000] || eLife || Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory.||UAS-MagIC, fluorescent Mg2+ reporter transgene | + | | [https://flybase.org/reports/FBrf0247921 FBrf0247921] || [https://pubmed.ncbi.nlm.nih.gov/33242000/ 33242000] || eLife || Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory.||UAS-MagIC, fluorescent Mg2+ reporter transgene |
|- | |- | ||
− | | FBrf0248065 || [https://pubmed.ncbi.nlm.nih.gov/33513131/ 33513131] || PLoS Comput. Biol. || Modeling invasion patterns in the glioblastoma battlefield.||Mathematical algoritjm for cytoneme/Tumor microtube expansion | + | | [https://flybase.org/reports/FBrf0248065 FBrf0248065] || [https://pubmed.ncbi.nlm.nih.gov/33513131/ 33513131] || PLoS Comput. Biol. || Modeling invasion patterns in the glioblastoma battlefield.||Mathematical algoritjm for cytoneme/Tumor microtube expansion |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247818 || [https://pubmed.ncbi.nlm.nih.gov/33444322/ 33444322] || PLoS Biol. || Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes.||We developed a new technique called mosaic analysis by gRNA-induced crossing-over (MAGIC) for mosaic analysis with unmodified genomes. We developed the MAGIC toolkit for chromosomal arm 2L. | + | | [https://flybase.org/reports/FBrf0247818 FBrf0247818] || [https://pubmed.ncbi.nlm.nih.gov/33444322/ 33444322] || PLoS Biol. || Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes.||We developed a new technique called mosaic analysis by gRNA-induced crossing-over (MAGIC) for mosaic analysis with unmodified genomes. We developed the MAGIC toolkit for chromosomal arm 2L. |
|} | |} | ||
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! ''FBrf number''!!''PubMed ID''!!''Journal''!!''Title''!!''Author description of technical advance'' | ! ''FBrf number''!!''PubMed ID''!!''Journal''!!''Title''!!''Author description of technical advance'' | ||
|- | |- | ||
− | | FBrf0247452 || [https://pubmed.ncbi.nlm.nih.gov/33273532/ 33273532] || Sci. Rep. || HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.|| Human genes expressed in breast cancer patients are screened for their ability to aberrate development of the Drosophila eye. Transgenic construct UAS-hRPS12 was described in this paper. | + | | [https://flybase.org/reports/FBrf0247452 FBrf0247452] || [https://pubmed.ncbi.nlm.nih.gov/33273532/ 33273532] || Sci. Rep. || HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.|| Human genes expressed in breast cancer patients are screened for their ability to aberrate development of the Drosophila eye. Transgenic construct UAS-hRPS12 was described in this paper. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247305 || [https://pubmed.ncbi.nlm.nih.gov/33196763/ 33196763] || J. Cell Biol. || centrocortin RNA localization to centrosomes is regulated by FMRP and facilitates error-free mitosis.|| Python-based code used to quantify object-to-object distances; e.g., mRNA to centrosomes | + | | [https://flybase.org/reports/FBrf0247305 FBrf0247305] || [https://pubmed.ncbi.nlm.nih.gov/33196763/ 33196763] || J. Cell Biol. || centrocortin RNA localization to centrosomes is regulated by FMRP and facilitates error-free mitosis.|| Python-based code used to quantify object-to-object distances; e.g., mRNA to centrosomes |
|- | |- | ||
− | | FBrf0247435 || [https://pubmed.ncbi.nlm.nih.gov/32815271/ 32815271] || EMBO Rep. || A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence in Drosophila.|| A single cell atlas of the proximal wing disc was built by single cell RNAseq. The myoblast, tracheal and epithelial cells associated with third instar larval wing disc were mapped. Diverse cell types along with specific markers were identified. | + | | [https://flybase.org/reports/FBrf0247435 FBrf0247435] || [https://pubmed.ncbi.nlm.nih.gov/32815271/ 32815271] || EMBO Rep. || A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence in Drosophila.|| A single cell atlas of the proximal wing disc was built by single cell RNAseq. The myoblast, tracheal and epithelial cells associated with third instar larval wing disc were mapped. Diverse cell types along with specific markers were identified. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247504 || [https://pubmed.ncbi.nlm.nih.gov/33168584/ 33168584] || Development || Recruitment of BAF to the nuclear envelope couples the LINC complex to endoreplication.|| Expansion microscopy for larval tissue | + | | [https://flybase.org/reports/FBrf0247504 FBrf0247504] || [https://pubmed.ncbi.nlm.nih.gov/33168584/ 33168584] || Development || Recruitment of BAF to the nuclear envelope couples the LINC complex to endoreplication.|| Expansion microscopy for larval tissue |
|- | |- | ||
− | | FBrf0247550 || [https://pubmed.ncbi.nlm.nih.gov/33321059/ 33321059] || Open Biol. || Identification of FoxP circuits involved in locomotion and object fixation in Drosophila.|| Several new fly lines. In particular: - a GFP-tagged FoxP gene - a null allele - a conditional UAS-gRNA-based knock-out line. | + | | [https://flybase.org/reports/FBrf0247550 FBrf0247550] || [https://pubmed.ncbi.nlm.nih.gov/33321059/ 33321059] || Open Biol. || Identification of FoxP circuits involved in locomotion and object fixation in Drosophila.|| Several new fly lines. In particular: - a GFP-tagged FoxP gene - a null allele - a conditional UAS-gRNA-based knock-out line. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0245063 || [https://pubmed.ncbi.nlm.nih.gov/31978587/ 31978587] || Insect Biochem. Mol. Biol. || Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides.|| nuclear localization signal (NLS) that isolated from D. suzukii transformer gene | + | | [https://flybase.org/reports/FBrf0245063 FBrf0245063] || [https://pubmed.ncbi.nlm.nih.gov/31978587/ 31978587] || Insect Biochem. Mol. Biol. || Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides.|| nuclear localization signal (NLS) that isolated from D. suzukii transformer gene |
|- | |- | ||
− | | FBrf0247583 || [https://pubmed.ncbi.nlm.nih.gov/33339500/ 33339500] || BMC Genet. || Identification and characterization of four Drosophila suzukii cellularization genes and their promoters.|| piggyBac constructs that express DeRed at embryonic stage | + | | [https://flybase.org/reports/FBrf0247583 FBrf0247583] || [https://pubmed.ncbi.nlm.nih.gov/33339500/ 33339500] || BMC Genet. || Identification and characterization of four Drosophila suzukii cellularization genes and their promoters.|| piggyBac constructs that express DeRed at embryonic stage |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247622 || [https://pubmed.ncbi.nlm.nih.gov/33377013/ 33377013] || STAR Protoc || Protocol for a Binary Choice Feeding Assay Using Adult, Axenic Drosophila.|| The protocol describes preparation of adult axenic Drosophila before monitoring their behavior in a two-choice feeding assay, where flies are confronted with an axenic versus a dead or alive bacteria-contaminated feeding solution. | + | | [https://flybase.org/reports/FBrf0247622 FBrf0247622] || [https://pubmed.ncbi.nlm.nih.gov/33377013/ 33377013] || STAR Protoc || Protocol for a Binary Choice Feeding Assay Using Adult, Axenic Drosophila.|| The protocol describes preparation of adult axenic Drosophila before monitoring their behavior in a two-choice feeding assay, where flies are confronted with an axenic versus a dead or alive bacteria-contaminated feeding solution. |
|- | |- | ||
− | | FBrf0247664 || [https://pubmed.ncbi.nlm.nih.gov/33320085/ 33320085] || eLife || A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling.|| CRISPR dual-gRNA transgenic targeting phosphoinositide signaling genes | + | | [https://flybase.org/reports/FBrf0247664 FBrf0247664] || [https://pubmed.ncbi.nlm.nih.gov/33320085/ 33320085] || eLife || A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling.|| CRISPR dual-gRNA transgenic targeting phosphoinositide signaling genes |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247494 || [https://pubmed.ncbi.nlm.nih.gov/33171098/ 33171098] || Dev. Cell ||Lineage-Resolved Enhancer and Promoter Usage during a Time Course of Embryogenesis.|| Tissue- and time-resolved chromatin accessibility during Drosophila embryogenesis | + | | [https://flybase.org/reports/FBrf0247494 FBrf0247494] || [https://pubmed.ncbi.nlm.nih.gov/33171098/ 33171098] || Dev. Cell ||Lineage-Resolved Enhancer and Promoter Usage during a Time Course of Embryogenesis.|| Tissue- and time-resolved chromatin accessibility during Drosophila embryogenesis |
|- | |- | ||
− | | FBrf0247517 || [https://pubmed.ncbi.nlm.nih.gov/33262328/ 33262328] || Nat. Commun. ||Deep learning suggests that gene expression is encoded in all parts of a co-evolving interacting gene regulatory structure.|| We apply deep learning on thousands of mRNA datasets to examine the genetic regulatory code controlling mRNA abundance in model organisms including Drosophila melanogaster, and show that in all organisms mRNA abundance can be predicted directly from DNA sequence. | + | | [https://flybase.org/reports/FBrf0247517 FBrf0247517] || [https://pubmed.ncbi.nlm.nih.gov/33262328/ 33262328] || Nat. Commun. ||Deep learning suggests that gene expression is encoded in all parts of a co-evolving interacting gene regulatory structure.|| We apply deep learning on thousands of mRNA datasets to examine the genetic regulatory code controlling mRNA abundance in model organisms including Drosophila melanogaster, and show that in all organisms mRNA abundance can be predicted directly from DNA sequence. |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247546 || [https://pubmed.ncbi.nlm.nih.gov/33112725/ 33112725] || Mol. Biol. Cell ||ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.|| UAS-driven regulatable fluorescent secretory cargo | + | | [https://flybase.org/reports/FBrf0247546 FBrf0247546] || [https://pubmed.ncbi.nlm.nih.gov/33112725/ 33112725] || Mol. Biol. Cell ||ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.|| UAS-driven regulatable fluorescent secretory cargo |
|- | |- | ||
− | | FBrf0247672 || [https://pubmed.ncbi.nlm.nih.gov/33337563/ 33337563] || FASEB J. ||Regulatory mechanism of daily sleep by miR-276a.|| 1. S2 cell culture and luciferase reporter assay; 2. AGO1 immunoprecipitation (AGO1 IP); 3. Immunofluorescence experiments; | + | | [https://flybase.org/reports/FBrf0247672 FBrf0247672] || [https://pubmed.ncbi.nlm.nih.gov/33337563/ 33337563] || FASEB J. ||Regulatory mechanism of daily sleep by miR-276a.|| 1. S2 cell culture and luciferase reporter assay; 2. AGO1 immunoprecipitation (AGO1 IP); 3. Immunofluorescence experiments; |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247745 || [https://pubmed.ncbi.nlm.nih.gov/33319644/ 33319644] || Fly ||PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup.|| Resource: website for downloading aligned genome sequences from Drosophila pseudoobscura species group | + | | [https://flybase.org/reports/FBrf0247745 FBrf0247745] || [https://pubmed.ncbi.nlm.nih.gov/33319644/ 33319644] || Fly ||PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup.|| Resource: website for downloading aligned genome sequences from Drosophila pseudoobscura species group |
|- | |- | ||
− | | FBrf0247752 || [https://pubmed.ncbi.nlm.nih.gov/33131172/ 33131172] || Insect Mol. Biol. ||Regulation of circadian behavioural output via clock-responsive miR-276b.|| S2 cell culture and luciferase reporter assay; AGO1 immunoprecipitation | + | | [https://flybase.org/reports/FBrf0247752 FBrf0247752] || [https://pubmed.ncbi.nlm.nih.gov/33131172/ 33131172] || Insect Mol. Biol. ||Regulation of circadian behavioural output via clock-responsive miR-276b.|| S2 cell culture and luciferase reporter assay; AGO1 immunoprecipitation |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247665 || [https://pubmed.ncbi.nlm.nih.gov/33234716/ 33234716] || Development ||Postsynaptic cAMP signalling regulates the antagonistic balance of Drosophila glutamate receptor subtypes.|| superresolution imaging of glutamate receptors were obtained with structured illumination microscopy (SIM) | + | | [https://flybase.org/reports/FBrf0247665 FBrf0247665] || [https://pubmed.ncbi.nlm.nih.gov/33234716/ 33234716] || Development ||Postsynaptic cAMP signalling regulates the antagonistic balance of Drosophila glutamate receptor subtypes.|| superresolution imaging of glutamate receptors were obtained with structured illumination microscopy (SIM) |
|- | |- | ||
− | | FBrf0247675 || [https://pubmed.ncbi.nlm.nih.gov/33077913/ 33077913] || Nat. Genet. ||Regulation of single-cell genome organization into TADs and chromatin nanodomains.|| Characterization of novel chromosomal structures in Drosophila embryonic cells, by combining 3D structured illumination microscopy (3D-SIM) and DAPI or FISH staining with a X Oligopaint probe (observation in a haploid context in sorted male embryos using a Y-GFP line). Ext. Fig.6 | + | | [https://flybase.org/reports/FBrf0247675 FBrf0247675] || [https://pubmed.ncbi.nlm.nih.gov/33077913/ 33077913] || Nat. Genet. ||Regulation of single-cell genome organization into TADs and chromatin nanodomains.|| Characterization of novel chromosomal structures in Drosophila embryonic cells, by combining 3D structured illumination microscopy (3D-SIM) and DAPI or FISH staining with a X Oligopaint probe (observation in a haploid context in sorted male embryos using a Y-GFP line). Ext. Fig.6 |
|- bgcolor="lightgrey" | |- bgcolor="lightgrey" | ||
− | | FBrf0247770 || [https://pubmed.ncbi.nlm.nih.gov/33437967/ 33437967] || STAR Protoc ||X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs.|| The methods paper describes detailed procedure for X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs to measure elemental distribution. Our report on the elemental distributions themselves was published in Hu et al. iScience 2020. | + | | [https://flybase.org/reports/FBrf0247770 FBrf0247770] || [https://pubmed.ncbi.nlm.nih.gov/33437967/ 33437967] || STAR Protoc ||X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs.|| The methods paper describes detailed procedure for X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs to measure elemental distribution. Our report on the elemental distributions themselves was published in Hu et al. iScience 2020. |
|} | |} | ||
Revision as of 12:39, 10 March 2021
Overview
Authors of the papers listed below suggest that they include a technical advance, new type of reagent, or resource likely to be useful for other researchers. Author suggestions have been collected via the Fast-Track Your Paper tool since October 2020, with those most recently submitted at the top. Please note, there will be a lag time before listed papers can be found on FlyBase and the submission date does not necessarily match the publication date of the paper.
Technical Advances
2021
Feb 2021
FBrf number | PubMed ID | Journal | Title | Author description of technical advance |
---|---|---|---|---|
FBrf0247772 | 33443210 | Proc. Natl. Acad. Sci. U.S.A. | Precise genome engineering in Drosophila using prime editing. | This paper describes reagents (e.g. transgenic flies, plasmids, cloning protocols) to perform prime editing in Drosophila melanogaster. |
FBrf0247854 | 31712250 | J. Lipid Res. | Lipidome-wide 13C flux analysis: a novel tool to estimate the turnover of lipids in organisms and cultures. | Metabolic flux analysis of lipids |
FBrf0247762 | 33149298 | Nature | Neuronal diversity and convergence in a visual system developmental atlas. | Developmental scRNA-seq atlas of the Drosophila optic lobe |
FBrf0247932 | 33025430 | Environ. Sci. Pollut. Res. Int. | Genes regulating development and behavior exhibited altered expression in Drosophila melanogaster exposed to bisphenol A: use of real-time quantitative PCR (qRT-PCR) and droplet digital PCR (ddPCR) in genotoxicity study. | Application of digital droplet PCR in gene expression study |
FBrf0247834 | 33346099 | Gene | Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination. | The transgenic sexing strains of Drosophila suzukii based on the testis-specific fluorescent expression. |
FBrf0247838 | 33490883 | FASEB Bioadv | Preference and detrimental effects of high fat, sugar, and salt diet in wild-caught Drosophila simulans are reversed by flight exercise. | New model of Flight exercise. New model of the Western Diet |
FBrf0247869 | 33495334 | Proc. Natl. Acad. Sci. U.S.A. | Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid. | Identification of neutral lipid-based secreted structures called microcarriers in the lumen of the male accessory gland, which can be stained by neutral lipid dyes like LipidTox and Nile Red. |
FBrf0247944 | 33514834 | Commun. Biol. | The level of oncogenic Ras determines the malignant transformation of Lkb1 mutant tissue in vivo. | Long-term in vivo imaging of Drosophila giant larvae tumor migration and invasion using SiMView microscopy. |
FBrf0247996 | 33532738 | STAR Protoc | Analyzing muscle structure and function throughout the larval instars in live Drosophila. | Methods to examine the musculature of the Drosophila larva. These methods include analysis of the muscle sarcomeres, particularly in the same larva throughout the different larval instars. This work contains methods to muscle structural changes to muscle functional changes. |
FBrf0248021 | 33537561 | Micropub Biol | Serum-free adapted Drosophila S2R+ line is amenable to RNA interference. | We verified that Drosophila S2R+ adapted to grow in serum-free media is amenable to RNAi interference-mediated gene knockdown. |
FBrf0248088 | 33563972 | Nat. Commun. | Dynamic sex chromosome expression in Drosophila male germ cells. | single cell genomics of testes |
FBrf0248123 | 33558234 | Proc. Natl. Acad. Sci. U.S.A. | The Krüppel-like factor Cabut has cell cycle regulatory properties similar to E2F1. | new reporter line and new null allele were generated in this paper |
FBrf0247919 | 33239786 | Nature | Neural circuit mechanisms of sexual receptivity in Drosophila females. | split-GAL4 lines labeling specific cell types |
FBrf0248082 | 33495632 | Nat. Cell Biol. | Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. | A translational fidelity reporter (UAS-Fluc-STOP-Nluc) - Drosophila mutant strains (RPS23-R67K, RPS26-KO) |
FBrf0248109 | 33558226 | Proc. Natl. Acad. Sci. U.S.A. | Multisensory interactions regulate feeding behavior in Drosophila. | The modified PER assay can deliver stimuli not only for taste, but also for other senses. we made a line that can express the hair plate in legs. |
FBrf0247921 | 33242000 | eLife | Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory. | UAS-MagIC, fluorescent Mg2+ reporter transgene |
FBrf0248065 | 33513131 | PLoS Comput. Biol. | Modeling invasion patterns in the glioblastoma battlefield. | Mathematical algoritjm for cytoneme/Tumor microtube expansion |
FBrf0247818 | 33444322 | PLoS Biol. | Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes. | We developed a new technique called mosaic analysis by gRNA-induced crossing-over (MAGIC) for mosaic analysis with unmodified genomes. We developed the MAGIC toolkit for chromosomal arm 2L. |
Jan 2021
FBrf number | PubMed ID | Journal | Title | Author description of technical advance |
---|---|---|---|---|
FBrf0247452 | 33273532 | Sci. Rep. | HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis. | Human genes expressed in breast cancer patients are screened for their ability to aberrate development of the Drosophila eye. Transgenic construct UAS-hRPS12 was described in this paper. |
FBrf0247305 | 33196763 | J. Cell Biol. | centrocortin RNA localization to centrosomes is regulated by FMRP and facilitates error-free mitosis. | Python-based code used to quantify object-to-object distances; e.g., mRNA to centrosomes |
FBrf0247435 | 32815271 | EMBO Rep. | A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence in Drosophila. | A single cell atlas of the proximal wing disc was built by single cell RNAseq. The myoblast, tracheal and epithelial cells associated with third instar larval wing disc were mapped. Diverse cell types along with specific markers were identified. |
FBrf0247504 | 33168584 | Development | Recruitment of BAF to the nuclear envelope couples the LINC complex to endoreplication. | Expansion microscopy for larval tissue |
FBrf0247550 | 33321059 | Open Biol. | Identification of FoxP circuits involved in locomotion and object fixation in Drosophila. | Several new fly lines. In particular: - a GFP-tagged FoxP gene - a null allele - a conditional UAS-gRNA-based knock-out line. |
FBrf0245063 | 31978587 | Insect Biochem. Mol. Biol. | Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides. | nuclear localization signal (NLS) that isolated from D. suzukii transformer gene |
FBrf0247583 | 33339500 | BMC Genet. | Identification and characterization of four Drosophila suzukii cellularization genes and their promoters. | piggyBac constructs that express DeRed at embryonic stage |
FBrf0247622 | 33377013 | STAR Protoc | Protocol for a Binary Choice Feeding Assay Using Adult, Axenic Drosophila. | The protocol describes preparation of adult axenic Drosophila before monitoring their behavior in a two-choice feeding assay, where flies are confronted with an axenic versus a dead or alive bacteria-contaminated feeding solution. |
FBrf0247664 | 33320085 | eLife | A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling. | CRISPR dual-gRNA transgenic targeting phosphoinositide signaling genes |
FBrf0247494 | 33171098 | Dev. Cell | Lineage-Resolved Enhancer and Promoter Usage during a Time Course of Embryogenesis. | Tissue- and time-resolved chromatin accessibility during Drosophila embryogenesis |
FBrf0247517 | 33262328 | Nat. Commun. | Deep learning suggests that gene expression is encoded in all parts of a co-evolving interacting gene regulatory structure. | We apply deep learning on thousands of mRNA datasets to examine the genetic regulatory code controlling mRNA abundance in model organisms including Drosophila melanogaster, and show that in all organisms mRNA abundance can be predicted directly from DNA sequence. |
FBrf0247546 | 33112725 | Mol. Biol. Cell | ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. | UAS-driven regulatable fluorescent secretory cargo |
FBrf0247672 | 33337563 | FASEB J. | Regulatory mechanism of daily sleep by miR-276a. | 1. S2 cell culture and luciferase reporter assay; 2. AGO1 immunoprecipitation (AGO1 IP); 3. Immunofluorescence experiments; |
FBrf0247745 | 33319644 | Fly | PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup. | Resource: website for downloading aligned genome sequences from Drosophila pseudoobscura species group |
FBrf0247752 | 33131172 | Insect Mol. Biol. | Regulation of circadian behavioural output via clock-responsive miR-276b. | S2 cell culture and luciferase reporter assay; AGO1 immunoprecipitation |
FBrf0247665 | 33234716 | Development | Postsynaptic cAMP signalling regulates the antagonistic balance of Drosophila glutamate receptor subtypes. | superresolution imaging of glutamate receptors were obtained with structured illumination microscopy (SIM) |
FBrf0247675 | 33077913 | Nat. Genet. | Regulation of single-cell genome organization into TADs and chromatin nanodomains. | Characterization of novel chromosomal structures in Drosophila embryonic cells, by combining 3D structured illumination microscopy (3D-SIM) and DAPI or FISH staining with a X Oligopaint probe (observation in a haploid context in sorted male embryos using a Y-GFP line). Ext. Fig.6 |
FBrf0247770 | 33437967 | STAR Protoc | X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs. | The methods paper describes detailed procedure for X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs to measure elemental distribution. Our report on the elemental distributions themselves was published in Hu et al. iScience 2020. |
2020
Dec 2020
FBrf number | PubMed ID | Journal | Title | Author description of technical advance |
---|---|---|---|---|
FBrf0247159 | 32917721 | G3 (Bethesda) | Analysis of Gal4 Expression Patterns in Adult Drosophila Females. | Analysis of Gal4 expression patterns of commonly used Gal4 drivers in adult females. |
FBrf0247182 | 33060173 | Genome Res. | Active retrotransposons help maintain pericentromeric heterochromatin required for faithful cell division. | We defined the origins of repeat-derived RNAs and their specific chromatin locations in Drosophila S2 cells using the global RNAâDNA interaction sequencing method(GRID-seq). |
FBrf0247192 | 33168136 | eLife | The Drosophila Individual Activity Monitoring and Detection System (DIAMonDS). | We have developed a novel automated Drosophila life cycle multi-phase transition (pupariation, eclosion, and death) timing detection and measurement system with high accuracy and high temporal resolution. |
FBrf0247218 | 33176146 | Cell Rep. | Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation. | A hydrogen peroxide probe was detected in vivo through a burnt fluorescent probe (TCFB, 2-Dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran) [Sedgwick et al., 2017]. |
FBrf0247316 | 33113043 | Apoptosis | Functional characterization of the Drosophila suzukii pro-apoptotic genes reaper, head involution defective and grim. | The binary expression of different pro-apoptotic genes using 2A peptides. |
FBrf0247326 | 33016195 | Fly | A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila. | Improved pipeline for CRISPR mediated genome editing in Drosophila species. Generation of new vector to make donor plasmids for homology-directed repair |
FBrf0247327 | 33234068 | Open Biol. | The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species-a potential new tool in entomology. | We describe a novel high throughput mass spectrometry approach to identifying species and sex by their metabolites. We show it works invertebrates generally, and can accurately distinguish closely related Drosophila (e.g. melanogaster and simulans) with potential for mass scoring |
FBrf0247119 | 32973040 | Genome Res. | Transposon expression in the Drosophila brain is driven by neighboring genes and diversifies the neural transcriptome. | Here we introduce TEchim, a new software that extracts chimeric sequencing reads that span transposon-gene breakpoints. In addition, we introduce scHardyWeinberg, a new approach to quantify co-expression levels in high-throughput single-cell sequencing data. |
FBrf0247148 | 33159074 | Nat. Commun. | A single-cell atlas and lineage analysis of the adult Drosophila ovary. | Single-cell RNA-sequencing dataset of the adult Drosophila ovary containing transcriptome profiles for all major ovarian cell types. |
FBrf0247254 | 33184447 | Commun. Biol. | A sensitive mNeonGreen reporter system to measure transcriptional dynamics in Drosophila development. | We developed an optimized version of the bright and fast-maturing fluorescent protein mNeonGreen to serve as a real-time, quantitative reporter of gene/enhancer expression. |
FBrf0247413 | 33274326 | Micropub Biol | Evidence of slightly increased Pol II pausing in UPF1-depleted Drosophila melanogaster cells. | DRB treatment followed by Pol II ChIP |
Nov 2020
FBrf number | PubMed ID | Journal | Title | Author description of technical advance |
---|---|---|---|---|
FBrf0246918 | 32955431 | eLife | AANAT1 functions in astrocytes to regulate sleep homeostasis. | Antibody |
FBrf0246897 | 33036271 | Biomedicines | Humanization of Drosophila Gαo to Model GNAO1 Paediatric Encephalopathies. | Endogenous locus was edited with a two-step CRISPR/Cas9-mediated replacement. |
FBrf0246907 | 32917765 | Biol. Open | Quantifying Drosophila adults with the use of a smartphone. | We optimized, tested, and introduced a new method of automated count of Drosophila adults using the SeedCounter mobile application. The application is available on all mobile devices based on the Android system, and it does not require any additional equipment. |
FBrf0246919 | 32783958 | J. Insect Physiol. | How to use the development of individual Drosophila larvae as a metabolic sensor. | We introduce a developmental 96-well plate assay to use individual Drosophila larvae as tool for metabolic studies. |
FBrf0247073 | 33077914 | Nat. Genet. | CHESS enables quantitative comparison of chromatin contact data and automatic feature extraction. | Novel method to compare 3D chromatin conformation data in Drosophila |
FBrf0247109 | 32814018 | Neuron | Synthesis of Conserved Odor Object Representations in a Random, Divergent-Convergent Network. | Comprehensive calcium imaging from all 2,000 Kenyon cells in the mushroom body at single cell resolution. |
FBrf0247160 | 33155978 | eLife | Modulation of flight and feeding behaviours requires presynaptic IP3Rs in dopaminergic neurons. | Dominant negative form of IP3R protein. It has three mutated amino acids which prevent ligand binding. IP3R being tetramer, requires IP3 binding to all monomers. Hence presence of even single IP3R-DN monomer in tetramer can render whole IP3R protein complex functionless. |
FBrf0247169 | 32916115 | Curr. Biol. | Collective Cell Sorting Requires Contractile Cortical Waves in Germline Cells. | use of Hydrogel for live-imaging of Drosophila ovaries |
FBrf0247175 | 33048531 | Anal. Chem. | Real-Time Measurement of Stimulated Dopamine Release in Compartments of the Adult Drosophila melanogaster Mushroom Body. | This paper uses fast-scan cyclic voltammetry at a carbon-fiber microelectrode to make real-time measurement of stimulated endogenous dopamine release in the mushroom body of adult Drosophila brains using various stimulation techniques |
FBrf0247184 | 33163946 | iScience | Transcription Factor Binding Affinities and DNA Shape Readout. | New measurements of Position weight Matrices (PWMs) Dinucleotide Position weight Matrices (DPWMs) for 13 Drosophila transcription factors and their DNA shape analysis for all shape features. |
FBrf0247019 | 33108408 | PLoS ONE | Three distinct mechanisms, Notch instructive, permissive, and independent, regulate the expression of two different pericardial genes to specify cardiac cell subtypes. | Sequence, features, and schematic of the pWattB-nlacZ reporter construct vector |
FBrf0247101 | 33117817 | Front. Cell Dev. Biol. | An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues. | A new method for immobilizing explanted Drosophila tissue in agarose for use in long-term live imaging. |
FBrf0247149 | 32955048 | Analyst | Improving serotonin fast-scan cyclic voltammetry detection: new waveforms to reduce electrode fouling. | Using new fast-scan cyclic voltammetry serotonin specific waveforms for in vivo detection of serotonin in Drosophila tissue. |
FBrf0247179 | 33090988 | PLoS Biol. | Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio. | We have developed a new protocol to indcue cell cycle arrest in early embryos, and deposited RNA-seq data for WT embryos and embryos arrested in cycle 12 for differernt amount of time. |
FBrf0247228 | 31960114 | Cell. Molec. Life Sci. | Loss of function in the Drosophila clock gene period results in altered intermediary lipid metabolism and increased susceptibility to starvation. | Analysis of the chronometabolome including lipids |
FBrf0247230 | 33177090 | Sci. Adv. | Persistent epigenetic reprogramming of sweet taste by diet. | Transgenic Targeted DamID construct for the gene polycomb-like (Pcl) under the control of a UAS. |
Oct 2020
FBrf number | PubMed ID | Journal | Title | Author description of technical advance |
---|---|---|---|---|
FBrf0246799 | 32013401 | Biochemistry | Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis. | Small molecules called "HACs" alter normal autoprocessing activity of hedgehog precursor protein. Instead of reacting with cholesterol (cholesterolysis), the HACs alter hedgehog precursor autoprocessing toward a side reaction with water (hydrolysis) |
FBrf0246922 | 32901612 | eLife | Topology-driven protein-protein interaction network analysis detects genetic sub-networks regulating reproductive capacity. | This paper shows how computational network analyses based on protein-protein interactions can be used to augment genetic screens to identify genes involved in essential developmental processes. The code are deposited in GitHub https://github.com/extavourlab/hpo_ova_eggL_screen. |
FBrf0246250 | 32652492 | iScience | PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants. | PhotoGal4 is a phytochrome B-based optogenetic switch for fine tuned spatiotemporal control of gene expression in Drosophila explants. PhotoGal4 activates and deactivates gene expression upon red- or far-red-light irradiation. |
FBrf0246808 | 32973185 | Sci. Rep. | A novel setup for simultaneous two-photon functional imaging and precise spectral and spatial visual stimulation in Drosophila. | We developed a unique visual stimulation setup to probe spectral, spatial, & temporal properties using neural activity imaging. Custom optical filters enabled simultaneous two-photon imaging & visual stimulation over a wide range of hues without compromising the temporal rates. |
FBrf0246889 | 32745561 | J. Insect Physiol. | CRISPR/Cas9 mediated disruption of the white gene leads to pigmentation deficiency and copulation failure in Drosophila suzukii. | New white gene mutant strains were generated in Drosophila suzukii and can be used as resources to study the corresponding phenotype. |
FBrf0246845 | 32958931 | Nat. Protoc. | CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells. | This protocol allows generation of homozygous mutant cell lines using an insertion cassette which autocatalytically generates insertion mutations in all alleles. Knockout cells generated using this method can be directly identified by PCR without a need for DNA sequencing. |
FBrf0246864 | 32759315 | J. Virol. | Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission. | Drosophila melanogaster line infected only with galbut virus (FoCo-17 line 30) |
FBrf0246967 | 33064752 | PLoS ONE | Comparative proteomics analysis of dietary restriction in Drosophila. | The isobaric tags for relative and absolute quantification (iTRAQ) technology was chosen to sequence the proteomes of Drosophila |