Difference between revisions of "FlyBase:Images"

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| [http://fly-fish.ccbr.utoronto.ca/ A database of Drosophila embryo and larvae mRNA localization patterns] || Database of 2D images and annotations of patterns of gene expression and RNA localisation in ''Drosophila'' embryos and larval tissues revealed by fluorescent RNA ''in situ'' hybridisation. || Krause Lab<br/> University of Toronto<br/> Toronto, Ontario, Canada
 
| [http://fly-fish.ccbr.utoronto.ca/ A database of Drosophila embryo and larvae mRNA localization patterns] || Database of 2D images and annotations of patterns of gene expression and RNA localisation in ''Drosophila'' embryos and larval tissues revealed by fluorescent RNA ''in situ'' hybridisation. || Krause Lab<br/> University of Toronto<br/> Toronto, Ontario, Canada
 
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|style="background: #efefef;"|[http://www.flyrnai.org/crispr2/ DRSC Find CRISPR Tool] ||style="background: #efefef;"| A tool for discovering gRNA targets located throughout the fly genome (release 5). Searchable by gene ID, symbol, or chromosome location. Includes options to modify mismatch stringency for off-targets.  ||style="background: #efefef;"| Drosophila RNAi Screening Center (DRSC)<br/> Harvard Medical School<br/> Boston, MA, USA
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|style="background: #efefef;"|[http://tomancak-srv1.mpi-cbg.de/DOT/main.html Dresden Ovary Table] ||style="background: #efefef;"|Database of 3D images and annotations of patterns of RNA localisation in ''Drosophila'' ovaries revealed by fluorescent RNA ''in situ'' hybridisation. ||style="background: #efefef;"| Tomancak lab<br/> Max Planck Institute of Molecular Cell Biology and Genetics<br/>Dresden, Germany
 
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| [http://cas9.cbi.pku.edu.cn/ Cas9 Design] || A CRISPR/Cas9 guide RNA design tool. Support for multiple species including fly (release 5), zebrafish, mouse, human, and others. || Center for Bioinformatics<br/> Peking University<br/> Beijing, China
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| [http://flweb.janelia.org/cgi-bin/flew.cgi Expression Patterns of GAL4 and LexA Driver Lines] || Database of images and annotation of patterns driven by systematically generated enhancer constructs (Rubin collection) in wing imaginal discs, larval nervous system and adult brain.|| Rubin, Truman, Doe, and Mann Labs<br/> Janelia Research Campus<br/> Ashburn, VA, USA
 
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|style="background: #efefef;"| [http://crispr.cos.uni-heidelberg.de/ CCTop] ||style="background: #efefef;"| CRISPR/Cas9 Target online predictor. Support for multiple species including <em>Drosophila</em> (release 5), zebrafish, mouse, human, and others. Notably large set of configurable parameters. ||style="background: #efefef;"| Center for Organismal Studies<br/> University of Heidelberg<br/>Heidelberg, Germany
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|style="background: #efefef;"| [http://enhancers.starklab.org/ Fly Enhancers] ||style="background: #efefef;"| Database of 2D images and annotation of patterns driven by systematically generated enhancer constructs (Dickson collection) in embryos.||style="background: #efefef;"| Stark Lab<br/> Research Institute of Molecular Pathology<br/>Vienna, Austria
 
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| [http://www.e-crisp.org/E-CRISP/designcrispr.html E-CRISP] || gRNA genome-wide target finder, searchable via  gene symbol or sequence. Relaxed, "medium", or strict search options. Support for multiple species including <em>Drosophila</em> (release 5), zebrafish, mouse, human, and others. ||Boutros lab, DKFZ<br/> Heidelberg, Germany
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| [http://insitu.fruitfly.org/cgi-bin/ex/search.pl?ftype=6&ftext=_* CRMs at BDGP] ||Database of images and annotation of patterns driven by systematically generated enhancer constructs (Rubin collection) in embryos.||Berkeley Drosophila Genome Project (BDGP)<br/> Lawrence Berkeley National Laboratory<br/>Berkeley, CA, USA
 
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|style="background: #efefef;"| [https://chopchop.rc.fas.harvard.edu/ CHOPCHOP] ||style="background: #efefef;"| Search for gRNA targets. Support for multiple species including <em>Drosophila</em> (release 5), zebrafish, mouse, human, and others. Configurable options for off-targets, PAM motifs, and 5' requirements. ||style="background: #efefef;"| Schier & Church Labs<br /> Harvard University<br /> Cambridge, MA, USA
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|style="background: #efefef;"| [http://rablibrary.mpi-cbg.de/cgi-bin/rab_overview.pl FLYtRAB] ||style="background: #efefef;"| Expression of 27 (all) endogenously tagged rab proteins in salivary glands, larval brain, wing discs, ovaries, testis and fat body imaged by high resolution confocal microscopy. Visualised in CATMAID. ||style="background: #efefef;"|Eaton and Tomancak Labs<br/> Max Planck Institute of Molecular Cell Biology and Genetics<br/>Dresden, Germany
 
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| [http://crispr.mit.edu/ CRISPR Design] ||Identification of gRNA sites genome wide. Support for multiple species including <em>Drosophila</em> (release 5), zebrafish, mouse, human, and others. || Zhang Lab<br /> MIT<br /> Boston, MA, USA
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| [http://www.flyprot.org/ The FLAnnotator] ||Database of images and annotations of Cambridge protein trap lines.||St Johnston Lab<br/>Wellcome Trust/Cancer Research UK Gurdon Institute<br/>Cambridge, UK
 
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|style="background: #efefef;"| [http://tefor.net/crispor/crispor.cgi CRISPOR] ||style="background: #efefef;"| A program that helps design, evaluate, and clone guide sequences for the CRISPR/Cas9 system. Support for multiple species including several <em>Drosophila</em> (release 5), zebrafish, mouse, human, and others.||style="background: #efefef;"| Tefor<br />France
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|style="background: #efefef;"| [http://flypush.imgen.bcm.tmc.edu/pscreen/rmce/ MiMIC RMCE lines] ||style="background: #efefef;"| Database of images of MiMIC lines in larval CNS (GFP staining).||style="background: #efefef;"| Bellen Lab</br> Baylor College of Medicine <br/>Houston, TX, USA
 
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| [http://www.virtualflybrain.org/site/vfb_site/home.htm Virtual Fly Brain] ||Database of images and annotations of Cambridge protein trap lines.||St Johnston Lab<br/>Wellcome Trust/Cancer Research UK Gurdon Institute<br/>Cambridge, UK
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Revision as of 18:58, 8 August 2015

Popular Resource Categories

All Resources CRISPR RNAi Stocks Antibodies Images

This image-based resources page has been generously contributed by Pavel Tomancak of the Max Planck Institute of Molecular Cell Biology and Genetics.

Imaged Based Resources

Resource Description Author/Source
Patterns of gene expression in Drosophila embryogenesis Database of 2D images and annotations of patterns of gene expression during Drosophila embryogenesis revealed by RNA in situ hybridisation. Berkeley Drosophila Genome Project (BDGP)
Lawrence Berkeley National Laboratory
Berkeley, CA, USA
A database of Drosophila embryo and larvae mRNA localization patterns Database of 2D images and annotations of patterns of gene expression and RNA localisation in Drosophila embryos and larval tissues revealed by fluorescent RNA in situ hybridisation. Krause Lab
University of Toronto
Toronto, Ontario, Canada
Dresden Ovary Table Database of 3D images and annotations of patterns of RNA localisation in Drosophila ovaries revealed by fluorescent RNA in situ hybridisation. Tomancak lab
Max Planck Institute of Molecular Cell Biology and Genetics
Dresden, Germany
Expression Patterns of GAL4 and LexA Driver Lines Database of images and annotation of patterns driven by systematically generated enhancer constructs (Rubin collection) in wing imaginal discs, larval nervous system and adult brain. Rubin, Truman, Doe, and Mann Labs
Janelia Research Campus
Ashburn, VA, USA
Fly Enhancers Database of 2D images and annotation of patterns driven by systematically generated enhancer constructs (Dickson collection) in embryos. Stark Lab
Research Institute of Molecular Pathology
Vienna, Austria
CRMs at BDGP Database of images and annotation of patterns driven by systematically generated enhancer constructs (Rubin collection) in embryos. Berkeley Drosophila Genome Project (BDGP)
Lawrence Berkeley National Laboratory
Berkeley, CA, USA
FLYtRAB Expression of 27 (all) endogenously tagged rab proteins in salivary glands, larval brain, wing discs, ovaries, testis and fat body imaged by high resolution confocal microscopy. Visualised in CATMAID. Eaton and Tomancak Labs
Max Planck Institute of Molecular Cell Biology and Genetics
Dresden, Germany
The FLAnnotator Database of images and annotations of Cambridge protein trap lines. St Johnston Lab
Wellcome Trust/Cancer Research UK Gurdon Institute
Cambridge, UK
MiMIC RMCE lines Database of images of MiMIC lines in larval CNS (GFP staining). Bellen Lab
Baylor College of Medicine
Houston, TX, USA
Virtual Fly Brain Database of images and annotations of Cambridge protein trap lines. St Johnston Lab
Wellcome Trust/Cancer Research UK Gurdon Institute
Cambridge, UK


CRISPR Stocks

Resource Description Author/Source
CRISPR system stocks for site-specific mutagenesis The Bloomington Stock Center's CRISPR/Cas9 page. Includes stocks for expression of both Cas9 nuclease and tracrRNA. Bloomington Drosophila Stock Center (BDSC)
Indiana University
Bloomington, IN, USA
CRISPR Fly Design Detailed information on stocks generated by the CRISPR Fly Design group at the MRC Laboratory of Molecular Biology (available from the BDSC). MRC Laboratory of Molecular Biology
Cambridge, UK
NIG-FLY CRISPR/Cas9 Reagents, protocols, and online tools developed for genome engineering in Drosophila. NIG-FLY
National Institute of Genetics
Mishima, Japan


CRISPR Vectors

Resource Description Author/Source
Addgene CRISPR/Cas9 Plasmids for use in insects A list of available Cas9 & gRNA expression plasmids for use in insects, including Drosophila. Addgene
Cambridge, MA, USA
CRISPR Fly Design Plasmids Descriptions and information regarding the plasmids created by the CRISPR Fly Design group (also deposited at Addgene). MRC Laboratory of Molecular Biology
Cambridge, UK
NIG-FLY CRISPR/Cas9 Reagents, protocols, and online tools developed for genome engineering in Drosophila. NIG-FLY
National Institute of Genetics
Mishima, Japan


Additional Useful CRISPR Links

Resource Description Author/Source
CRISPR Fly Design Reagents, protocols, and results from fly CRISPR/Cas9 experiments. MRC Laboratory of Molecular Biology
Cambridge, UK
flyCRISPR An overview and resource collection for CRISPR gene editing in Drosophila. O'Connor-Giles, Wildonger, and Harrison Labs
University of Wisconsin-Madison
WI, USA
Insect Genomic Technologies Research Coordination Network Discussion and dissemination of advanced technologies for genome modification of insects through symposia, technical workshops, and training fellowships. Institute for Bioscience and Biotechnology Research
University of Maryland
Rockville, MD, USA
FlyRNAi Blog, CRISPR tags A research blog tracking functional genomics techniques in Drosophila, filtered for keyword: CRISPRs. Drosophila RNAi Screening Center (DRSC)
Harvard Medical School
Boston, MA, USA
DRSC Single Cell Cloning Protocol Single-cell cloning method useful for Drosophila cell CRISPR gene editing. Drosophila RNAi Screening Center (DRSC)
Harvard Medical School
Boston, MA, USA
OXfCRISPR Information about protocols and reagents, new refinements of techniques, useful links to other tools, websites, and discussion groups. Liu lab
University of Oxford
Oxford, UK


Selected recent CRISPR method reviews

Selected recent CRISPR method reports for tissue culture cells

Selected recent CRISPR method reports for in vivo


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