Difference between revisions of "FlyBase:Drosophila Material Resources"

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* '''Bloomington Stock Center'''<br />Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at [http://flystocks.bio.indiana.edu/ http://flystocks.bio.indiana.edu]
 
* '''Bloomington Stock Center'''<br />Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at [http://flystocks.bio.indiana.edu/ http://flystocks.bio.indiana.edu]
 
* '''CRISPR Stocks - Bloomington Stock Center'''<br />CRISPR system stocks for site-specific mutagenesis can be obtained at http://flystocks.bio.indiana.edu/Browse/misc-browse/CRISPR.htm/
 
* '''CRISPR Stocks - Bloomington Stock Center'''<br />CRISPR system stocks for site-specific mutagenesis can be obtained at http://flystocks.bio.indiana.edu/Browse/misc-browse/CRISPR.htm/
 +
* '''CRISPR Stocks - CRISPR fly design cas9 stocks description'''<br />  http://www.crisprflydesign.org/flies//
 
* '''DrosDel Project'''<br />Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at http://www.drosdel.org.uk/
 
* '''DrosDel Project'''<br />Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at http://www.drosdel.org.uk/
 
* '''Drosophila Gene Search Project'''<br />Information about ordering P{GS} lines can be found at http://kyotofly.kit.jp/stocks/documents/GS_lines.html
 
* '''Drosophila Gene Search Project'''<br />Information about ordering P{GS} lines can be found at http://kyotofly.kit.jp/stocks/documents/GS_lines.html

Revision as of 17:37, 23 November 2015

Genomic Clones

BAC Genomic clones and Filters

  • One BAC clone library made from DNA of the same y1 ; cn1 bw1 sp1 stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
  • BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
  • Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
  • For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org

P1 Genomic Clones

  • As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
  • For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.

Cosmid Genomic Clones and Filters

The members of the European Drosophila Genome mapping consortium are:

  • F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
    Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
  • D.M. Glover and R.D.C. Saunders,
    Department of Biochemistry, University of Dundee, Dundee, Scotland.
  • M. Ashburner,
    Department of Genetics, University of Cambridge, Cambridge, England.
  • J. Modolell,
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
    with initial funding from the Fondation Schlumberger.

Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.

Contact:

Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm

cDNA (EST Clones and Libraries)

Arrays and Primers

Stocks

CRISPR Vectors

  • CRISPR/Cas Vectors from Simon Bullock Lab
    A set tools for efficient germline and somatic genome engineering in Drosophila melanogaster, available at Addgene plasmid repository https://www.addgene.org/Simon_Bullock/, Cambridge, MA, USA

Expression Vectors

  • Drosophila Gateway Vectors
    A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC
  • ExpreS2ion Biotechnologies
    ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. ExpreS2ion Biotechnologies

Cell Lines and Hybridomas

  • Drosophila Genomics Resource Center (DGRC)
    Collects and distributes cell lines. More information is available at http://dgrc.cgb.indiana.edu/
  • Developmental Studies Hybridoma Bank
    The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at http://dshb.biology.uiowa.edu
  • ATCC
    The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at http://www.atcc.org/

Antibodies

Transgenic, Targeted Mutation, and Other Services

  • Aktogen behaviour genetic services
    Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. http://www.aktogen.com/
  • BestGene Transgenic Service
    The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at http://www.thebestgene.com/
  • C-CAMP Centre for Cellular and Molecular Platforms
    A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at http://ccamp.res.in/Fly-facility
  • Drosophila TILLING Project
    The goal of Fly-TILL is to rapidly deliver to the Drosophila community EMS-induced mutations in requested genes via high-throughput reverse genetic screens. More information is available at http://tilling.fhcrc.org/fly/Welcome_to_Fly-TILL.html
  • Fly Facility
    Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at http://www.fly-facility.com/
  • Genetic Services
    Genetic Services provides the Drosophila community with services including transgenic production, injection work, genetic screening, stock creation, construct production and consulting. More information is available at http://www.geneticservices.com
  • GenetiCorps Custom Injection Services
    GenetiCorps offers high quality fly injection services to the Drosophila research community. For more information, please visit http://www.geneticorps.com/
  • Genetivision, Inc.
    Genetivision offers a transgenic fly service for both delta 2-3 transposase-mediated P-element transgenesis and Phi-C31 mediated site-specific integration. More information is available at http://www.genetivision.com/
  • The Genome Engineering Production Group
    The GEPG at Harvard Medical School currently builds custom CRISPR sgRNA constructs for knock-out, knock-in or other projects. http://gepg.hms.harvard.edu/
  • GenScript
    A US based company offering gene construction, mutagenesis and subcloning services, as well as a large array of other products and services. http://www.genscript.com/
  • Rainbow Transgenic Flies
    Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at http://www.rainbowgene.com/
  • Transgenic Drosophila Fly Core
    The Cutaneous Biology Research Center (CRBC) Fly Core at Massachusetts General Hospital/Harvard Medical School provides microinjection services to create transgenic Drosophila fly strains. More information is available at http://www.flycore.com
  • Union Biometrica
    COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at http://www.unionbio.com/
  • VANEDIS Drosophila injection service
    The VANEDIS Drosophila injection service injects embryos with your construct of interest. More information is available at http://www.vanedis.no

RNA

Clontech
Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2

RNAi

Suggest a tool or resource

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