FlyBase:RNAi

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RNAi Design/Analysis Tools

Resource	Description	Author/Source
E-RNAi	A tool for the design and evaluation of RNAi reagents. It can be used to design and evaluate long dsRNAs (including esiRNAs) as well as siRNAs. Support for multiple species including Drosophila (release 5), mouse, human, and others.	Boutros lab DKFZ Heidelberg, Germany
NEXT-RNAi	Software for the design and evaluation of genome-wide RNAi libraries. It performs all steps; from the prediction of specific and efficient RNAi target sites to the visualization of designed reagents in their genomic context.	Boutros lab DKFZ Heidelberg, Germany
SnapDragon	A tool for the design of long dsRNAs for specific cell-based gene knockdown in Drosophila melanogaster via an RNAi approach.	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA

RNAi Stocks for in vivo studies (fly stocks)

Resource	Description	Author/Source
UP-TORR, Updated Targets of RNAi Reagents	A lookup tool for searching BDSC, TRiP, VDRC and NIG-FLY in vivo RNAi collections.	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA
BDSC RNAi Home Page	Links to lists and sublists of RNAi and miRNA sponge insertions at Bloomington. Most, but not all, of the insertions are under the control of UAS and therefore require GAL4 for expression.	Bloomington Drosophila Stock Center (BDSC) Indiana University Bloomington, IN, USA
NIG-FLY	NIG-FLY in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request.	NIG-FLY National Institute of Genetics Mishima, Japan
TRiP Transgenic RNAi Project	The TRiP Stock Collection contains over 9,000 lines, 1,575 of which correspond to orthologs of human-disease-associated genes. The stocks can be obtained from the BDSC.	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA
THFC, Tsinghua Fly Center	THFC RNAi Stock Collection for triggering RNAi in soma and germline	Tsinghua Fly Center Beijing, China
VDRC, Vienna Drosophila Resource Center	The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries	Vienna Drosophila Resource Center (VDRC) Vienna, Austria

Cell-based RNAi Reagents

Resource	Description	Author/Source
DRSC Libraries	Genome-wide libraries for cell-based RNAi screening and validation (on-site or shipped).	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA
Division of Signaling and Functional Genomics	Genome-wide libraries for cell-based RNAi screening and validation. Includes the resource site GenomeRNAi.	Boutros lab DKFZ Heidelberg, Germany
Sheffield RNAi Screening Facility	The SRSF at Biomedical Science at the University of Sheffield advertises low-cost RNAi screens in Drosophila cells.	University of Sheffield Sheffield, UK

RNAi Screening Centers

Resource	Description	Author/Source
DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR)	DRSC/TRiP Functional Genomics Resources at Harvard Medical School provides high throughput RNAi screens.	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA
NYU RNAi Screening and Automation Core	The NYU RNAi Core Facility at the Langone Medical Center in New York provides high-throughput RNAi screens. Drosophila screens are offered in collaboration with the DRSC.	NYU RNAi Core New York, NY, USA
Sheffield RNAi Screening Facility	The SRSF provides a service for whole-genome RNAi screens in Drosophila cells.	SRSF University of Sheffield Sheffield, UK

RNAi Results, Validation and Phenotypes

Resource	Description	Author/Source
GenomeRNAi	GenomeRNAi is a database containing phenotypes from cell-based and in vivo RNA interference (RNAi) screens in Drosophila.	GenomeRNAi DKFZ Heidelberg, Germany
RSVP	RNAi Stock Validation and Phenotypes for in vivo RNAi studies.	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA
Cell-Based RNAi Screen Summary	A summary of public cell-based DRSC screens.	DRSC/TRiP Functional Genomics Resources (DRSC/TRiP-FGR) Harvard Medical School Boston, MA, USA

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