FlyBase:Interaction Report
Last Updated: 24 June 2015
The Interaction Report contains information for the physical interaction of two genes' products (protein or RNA).
This is a field-by-field guide to the information provided in the Interaction Report.
General Information
| Interaction Type | Specifies the type of interaction. Currently, only "physical association" between the products of two genes (protein-protein or RNA-protein) is reported. |
| Interacting Genes | Indicates the two genes involved in the interaction. Currently, only interactions involving two D. melanogaster genes are reported. Only pair-wise interactions are reported. |
| FlyBase ID | The FlyBase identifier number of the physical interaction, used to uniquely identify the interaction in the database. |
Interaction Network
For each gene in the interaction, a link to the FlyBase "Interactions Browser" is provided that will display all physical and genetic interactions for that gene.
Reported Interactions
Each reported instance of the interaction between the two genes is detailed in its own section. There is at least one reported interaction instance per interaction report.
| <Section title> | The section title is a unique identifier for each reported instance of the interaction. It typically contains the FlyBase "FBrf" reference ID for the publication from which the interaction was curated, an arbitrary numerical identifier, and a suffix that is suggestive of the assay methods used (e.g., coIP indicates coimmunoprecipitation, Y2H indicates yeast two hybrid). |
| Reference | The publication in which this particular instance of the interaction was reported. |
| Description | The type of interaction. Currently, only "physical association" is reported. |
| Assay | The methods used to detect the interactions and identify the interacting participants. The terms listed are part of the PSI-MI controlled vocabulary used by FlyBase, which can be viewed on the FlyBase Vocabularies page. |
| Collection | The large scale dataset/collection from which the interaction instance was derived, if applicable. |
| Source/Stage | The stage and tissue in which this interaction instance was observed. |
| Cell line used | The Drosophila cell line from in which this interaction instance was observed. |
Participants
Information about each of the two gene products involved in the reported interaction instance.
| Corresponds to | The gene name and the gene product (protein or RNA). |
| Reported as | The synonym used by the authors in referring to the gene product. |
| Role | The experimental role of the gene product in the reported interaction (for example, bait, prey, enzyme, enzyme target, neutral component). |
| Note | Any additional notes on the gene product, especially any epitope tags or labels used in the experiment. |
Experimental entities
Information on other experimental entities in the reported interaction instance, such as peptides identified or reagents used.
| Corresponds to | Name of the entity. |
| Identifier | A unique identifier for the entity. |
| Reported | The synonym used by the authors in referring to the entity. |
| Role | The experimental role of the entity in the reported interaction (for example, reagent or identified peptide). |
Subregions with role in interaction
Information on subregions of the gene products important to the reported interaction instance.
| Corresponds to | Name of the gene product. |
| Description | Description of the region of interest as provided by the reference. |
| Role | The experimental role of the entity in the reported interaction (for example, sufficient binding site, necessary binding site, mutation disrupting mutation). |
| Coordinates | Coordinates or sequence of the region of interest. |
| Notes | Additional notes on the region of interest. |
Isoform-specific participants
Information on isoform-specificity of the reported interaction instance.
| Corresponds to | Name of the gene product. |
| Description | Description of the isoform provided by the reference. |
| Role | The experimental role of the entity in the reported interaction. |
| Notes | Additional notes on the isoform-specificity of the interaction. |
Comments concerning this interaction
Additional comments on the reported interaction instance. This includes comments on the source of the two gene products tested, the experimental methods used and the authors' interpretation of the results.
References
A list of publications that discuss all reported instances of the interaction, subdivided into fields by type of publication.