FlyBase:Interaction Report

Last Updated: 24 June 2015

The Interaction Report contains information for the interaction between two genes.

This is a field-by-field guide to the information provided in the Interaction Report.

FlyBase attributes data to the publication that reported it, so that users can easily refer back to the original publication if they wish. Thus, where possible in the fields below, the publication(s) that are the source of the information are listed, typically in parentheses to the right of the data.

General Information

Interaction Type	Specifies the type of interaction. Currently, only "physical association" between the products of two genes (protein-protein or RNA-protein) is reported.
Interacting Genes	Indicates the two genes involved in the interaction. Currently, only interactions involving two D. melanogaster genes are reported. Only pair-wise interactions are reported.
FlyBase ID	The FlyBase identifier number of the interaction group, used to uniquely identify the interaction group in the database.

Interaction Network

For each gene in the interaction, a link to the FlyBase "Interactions Browser" is provided that will display all interactions for that gene.

Reported Interactions

Each reported instance of the interaction between the two genes is detailed in its own section. There will be at least one reported interaction instance, but there may be many distinct interaction instances.

<Section title>	The section title is a unique identifier for each reported instance of the interaction. It typically contains the FlyBase publication ID from which the interaction was curated, an arbitrary numerical identifier, and a suffix that is suggestive of the assay methods used (e.g., coIP indicates coimmunoprecipitation, Y2H indicates yeast two hybrid).
Reference	The publication in which this particular instance of the interaction was reported.
Description	The type of interaction. Currently, only "physical association" is reported.
Assay	The methods used to detect the interactions and identify the interacting participants. The terms listed are part of the PSI-MI controlled vocabulary used by FlyBase, which can be viewed on the FlyBase Vocabularies page.
Collection	The large scale dataset/collection from which the interaction instance was derived, if applicable.
Source/Stage	The stage and tissue in which this interaction instance was observed.
Cell line used	The Drosophila cell line from in which this interaction instance was observed.

Participants

Information about each of the two gene products involved in the reported interaction instance.

Corresponds to	The gene name and the gene product (protein or RNA).
Reported as	The synonym used by the authors in referring to the gene product.
Role	The experimental role of the gene product in the reported interaction (for example, bait, prey, enzyme, enzyme target, neutral component).
Note	Any additional notes on the gene product, especially any epitope tags or labels used in the experiment.

Experimental entities

Information on other experimental entities in the reported interaction instance, such as peptides identified or reagents used.

Corresponds to	Name of the entity.
Identifier	A unique identifier for the entity.
Reported	The synonym used by the authors in referring to the entity.
Role	The experimental role of the entity in the reported interaction (for example, reagent or identified peptide).

Subregions with role in interaction

Information on subregions of the gene products important to the reported interaction instance.

Corresponds to	Name of the gene product.
Description	Description of the region of interest as provided by the reference.
Role	The experimental role of the entity in the reported interaction (for example, sufficient binding site, necessary binding site, mutation disrupting mutation).
Coordinates	Coordinates or sequence of the region of interest.
Notes	Additional notes on the region of interest.

Isoform-specific participants

Information on isoform-specificity of the reported interaction instance.

Corresponds to	Name of the gene product.
Description	Description of the isoform provided by the reference.
Role	The experimental role of the entity in the reported interaction.
Notes	Additional notes on the isoform-specificity of the interaction.	Comments concerning this interaction Additional comments on the reported interaction instance. This includes comments on the source of the two gene products tested, the experimental methods used and the authors' interpretation of the results. References A list of publications that discuss the interaction, subdivided into fields by type of publication.