Roote & Prokop Mating Scheme Training Package ... Mar 2013
New to flies and need to learn how to design a genetic mating scheme? Thanks to John Roote and Andreas Prokop, there is now a four-part training package available. Explained in their recent G3 paper, this package is designed to ensure that basic knowledge on all key areas is covered in a concise and informative manner. First, a manual introduces the trainee to key topics including fly history, rationale for mating schemes, fly handling, markers and balancers, and transgenic technologies. This self-study section is followed by a practical training session on gender and marker selection, introducing real flies under the dissecting microscope. Next, through self-study of a PowerPoint presentation, trainees are guided step-by-step through a mating scheme. Finally, to consolidate knowledge, trainees are asked to design similar mating schemes reflecting routine tasks in a fly laboratory.
More info about the resource can be found here:
GenBank Release corresponding to FlyBase release 5.48 ... Jan 2013
An update to the Drosophila melanogaster annotations was published by NCBI in January 2013. This update is reflected in GenBank (Jan 3), RefSeq (Jan 16), and Entrez Gene (Jan 17) records. This GenBank release corresponds to release 5.48 of the D. melanogaster genome annotation except for the changes noted below. We plan to continue to submit D. melanogaster annotation updates to NCBI approximately once a year. Exceptions to this timetable will occur when the assembly of the reference D. melanogaster genome is updated and annotations are migrated onto the new assembly.
Differences between the January 2013 GenBank annotations and those in release 5.48 of FlyBase:
The following transcript isoforms are not present in the GenBank submission and were subsequently deleted in annotation release 5.49 of Dmel in FlyBase as genbank validation and curator evaluation determined they were not valid models:
CG17273-RB CG6044-RF su(f)-RH
The following protein isoforms (and their transcripts) were deleted from the Genbank submission and a new isoform (listed) was created due to inadvertently introduced annotation errors that were subsequently corrected and are also reflected in release 5.49 of Dmel in FlyBase:
CG14869-PD deleted - CG14869-PE created CG43248-PB deleted - CG43248-PC created CG8564-PB deleted - CG8564-PC created RhoGAP18B-PG deleted - RhoGAP18B-PH created
The following protein isoforms (and their transcripts) were renamed respectively in the GenBank submission and subsequent FlyBase releases as the listed suffix had been previously used for a different isoform in a previous GenBank submission:
CG8290-PC renamed to CG8290-PD CG9314-PB renamed CG9314-PC CG9855-PB renamed to CG9855-PC Pcmt-PB renamed Pcmt-PC cas-PB renamed cas-PC alien-PC renamed alien-PD numb-PC renamed numb-PD CG30020-PB renamed CG30020-PC Su(dx)-PD renamed Su(dx)-PF Fbp1-PC renamed Fbp1-PD Qtzl-PB renamed Qtzl-PC (CG32758-PB) renamed CG32758-PC CG8908-PC renamed CG8908-PE grp-PE renamed grp-PF CG31710-PB renamed CG31710-PD Sur-8-PC renamed Sur-8-PE Sur-8-PD renamed Sur-8-PF CG13135-PB renamed CG13135-PC CG31139-PB renamed CG31139-PC CG1340-PB renamed CG1340-PC Syt14-PB renamed Syt14-PD toy-PB renamed toy-PC Tm2-PD renamed Tm2-PF Uba1-PB renamed Uba1-PC bun-PI renamed bun-PO CG2614-PB renamed CG2614-PC dik-PB renamed dik-PC CG17724-PC renamed CG17724-PE CG11714-PB renamed CG11714-PC CG12788-PB renamed CG12788-PD
FlyBase 2013 Release Schedule ... Nov 2012
Drosophila Board White Paper 2012 ... Jun 2012