Difference between revisions of "FlyBase:Drosophila Material Resources"

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* '''Berkeley Screening Center'''<br /> The BSC at the UC Berkeley Department of Molecular and Cell Biology provides access to and training for high-throughput technology. The BSC has several mammalian libraries and in collaboration with the DRSC, offers full-genome and sub-library dsRNA screening in <span class="italic">Drosophila''' cells. http://bsc.berkeley.edu
* '''Drosophila RNAi Screening Center'''<br /> The Drosophila RNAi Screening Center at Harvard Medical School provides high throughput RNAi screens. http://flyrnai.org/RNAi_index.html
* '''FLIGHT'''<br /> FLIGHT is an online compilation of <span class="italic">Drosophila''' RNAi screens. http://flight.icr.ac.uk/
* '''Gene Silencing Platform'''<br /> The GSP at CIC bioGUNE provides cell-based screens using RNAi. http://cicbiogune.es/secciones/investigacion/plataformas_tecnologicas.php?idioma=en
* '''Geneservice'''<br /> Geneservice, in collaboration with Cyclacel Limited, offers a collection of 13,600 RNAi constructs in its Drosophila genome RNAi library. http://www.geneservice.co.uk/products/rnai/Dros_RNAi.jsp
* '''Helmholtz RNAi Screening Platform'''<br /> The Helmholtz RNAi Screening Platform at the University of Heidelberg provides RNAi screening in and computational tools for <span class="italic">Drosophila''' and human cells. http://b110-wiki.dkfz.de/signaling/wiki/display/rnaiwiki.
* '''NIG-FLY RNAi Resources'''<br /> NIG-Fly in Mishima, Japan is part of a consortium with Kyoto-Fly and two other stock centers, and distributes RNAi flies on request. [http://www.shigen.nig.ac.jp/fly/nigfly/index.jsp http://www.shigen.nig.ac.jp/fly/nigfly/0]
* '''NYU RNAi Core'''<br /> The NYU RNAi Core Facility at the Langone Medical Center in New York provides high-throughput RNAi screens. <span class="italic">Drosophila''' screens are offered in collaboration with the DRSC. http://rnainy.med.nyu.edu/
* '''Open Biosystems'''<br /> Open Biosystems provides the Drosophila RNAi Collection version 1.0, a collection of dsDNA constructs for generation of dsRNA. http://www.openbiosystems.com/RNAi/DrosophilaRNAicollections/
* '''Sheffield RNAi Screening Facility'''<br /> The SRSF at Biomedical Science at the University of Sheffield advertises low-cost RNAi screens in <span class="italic">Drosophila''' cells. http://www.rnai.group.shef.ac.uk/
* '''Transgenic RNAi Project'''<br /> TRiP at the Harvard Medical School plans to generate 6,250 transgenic RNAi lines targeted to attP2 on chromosome 3. Stocks are distributed through BDSC. http://www.flyrnai.org/TRiP-HOME.html
* '''Vienna Drosophila RNAi Center'''<br /> The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic <span class="italic">Drosophila''' RNAi libraries. http://www.vdrc.at
=Transgenic, Targeted Mutation, and Other Services=
=Transgenic, Targeted Mutation, and Other Services=

Revision as of 15:55, 17 April 2014

Genomic Clones

BAC Genomic clones and Filters

  • One BAC clone library made from DNA of the same y1 ; cn1 bw1 sp1 stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
  • BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
  • Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
  • For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org

P1 Genomic Clones

  • As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
  • For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.

Cosmid Genomic Clones and Filters

The members of the European Drosophila Genome mapping consortium are:

  • F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
    Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
  • D.M. Glover and R.D.C. Saunders,
    Department of Biochemistry, University of Dundee, Dundee, Scotland.
  • M. Ashburner,
    Department of Genetics, University of Cambridge, Cambridge, England.
  • J. Modolell,
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
    with initial funding from the Fondation Schlumberger.

Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.


Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm

cDNA (EST Clones and Libraries

Arrays and Primers

  • Affymetrix
    The Affymetrix GeneChip Drosophila Genome Array for gene expression analysis based on Release 3.1 annotations can be obtained at http://www.affymetrix.com/products/arrays/specific/fly_2.affx

    The Affymetrix Drosophila Tiling 2.0R Array based on Genome Release 5 with comprehensive genome coverage at 38bp resolution can be obtained here: http://www.affymetrix.com/products/arrays/specific/drosophila_tiling2_0r.affx
  • Drosophila Genomics Resource Center (DGRC)
    Produces and distributes DNA microarray slides. More information is available at http://dgrc.cgb.indiana.edu/
  • FlyChip
    FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/
  • FlyPrimerBank
    An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at www.flyrnai.org/flyprimerbank
  • International Drosophila Array Consortium (INDAC)
    An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at http://www.indac.net/
  • UK Drosophila Affymetrix Microarray Service
    A publicly funded microarray service for the UK Drosophila research community. More information is available at http://www.mblab.gla.ac.uk/igf/


Expression Vectors

  • Drosophila Gateway Vectors
    A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC
  • ExpreS2ion Biotechnologies
    ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. ExpreS2ion Biotechnologies

Cell Lines and Hybridomas

  • Drosophila Genomics Resource Center (DGRC)
    Collects and distributes cell lines. More information is available at http://dgrc.cgb.indiana.edu/
  • Developmental Studies Hybridoma Bank
    The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at http://dshb.biology.uiowa.edu
  • ATCC
    The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at http://www.atcc.org/



Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2


=Transgenic, Targeted Mutation, and Other Services=