Difference between revisions of "FlyBase:Drosophila Material Resources"

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(cDNA (EST Clones and Libraries)
(cDNA (EST Clones and Libraries)
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=cDNA (EST Clones and Libraries=
 
=cDNA (EST Clones and Libraries=
* <span class="bold">Addgene</span><br />Addgene helps labs archive and share their plasmids that have appeared in publications.<br />[http://www.addgene.org/pgvec1?f=c&cmd=browse http://www.addgene.org/pgvec1?f=c&amp;cmd=browse]
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* '''Addgene'''<br />Addgene helps labs archive and share their plasmids that have appeared in publications.<br />[http://www.addgene.org/pgvec1?f=c&cmd=browse http://www.addgene.org/pgvec1?f=c&amp;cmd=browse]
* <span class="bold">Berkeley Drosophila Genome Project</span><br />The information is provided by BDGP. If you don't find the information you need here check http://www.fruitfly.org/about/materials/ for possible updates.
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* '''Berkeley Drosophila Genome Project'''<br />The information is provided by BDGP. If you don't find the information you need here check http://www.fruitfly.org/about/materials/ for possible updates.
* <span class="bold">Brian Oliver Laboratory</span><br />Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from the Geneservice in the UK. <br />Ordering information can be found at http://www.geneservice.co.uk/products/cdna/dros_cdna.jsp
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* '''Brian Oliver Laboratory'''<br />Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from the Geneservice in the UK. <br />Ordering information can be found at http://www.geneservice.co.uk/products/cdna/dros_cdna.jsp
* <span class="bold">cDNA library aliquots</span><br />Aliquots from a number of the BDGP cDNA libraries are available from the labs on this [http://www.fruitfly.org/about/materials/cDNA-list.html list].
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* '''cDNA library aliquots'''<br />Aliquots from a number of the BDGP cDNA libraries are available from the labs on this [http://www.fruitfly.org/about/materials/cDNA-list.html list].
* <span class="bold">Clontech</span><br />Clontech (BD Biosciences) offers ''D. melanogaster'' embryo and larva 5'-STRETCH cDNA libraries at http://www.clontech.com/products/cat/HTML/1186.shtml
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* '''Clontech'''<br />Clontech (BD Biosciences) offers ''D. melanogaster'' embryo and larva 5'-STRETCH cDNA libraries at http://www.clontech.com/products/cat/HTML/1186.shtml
* <span class="bold">Drosophila Gene Collection (DGC)</span><br />Contact [http://bacpac.chori.org/ <span class="bold">BACPAC Resources</span>], [http://www.openbiosystems.com/Genomics/Model Organism Resources/Fly Resources/Drosophila Gene Collection (DG/  <span class="bold">Open Biosystems</span>], [http://www.geneservice.co.uk/products/cdna/DGC.jsp <span class="bold">Geneservice</span>], or the [http://dgrc.cgb.indiana.edu/ <span class="bold">DGRC</span>]<br />for clones in [http://www.fruitfly.org/DGC/DGC.txt DGCr1.0] or [http://www.fruitfly.org/DGC/dgc2.txt DGCr2.0].
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* '''Drosophila Gene Collection (DGC)'''<br />Contact [http://bacpac.chori.org/ '''BACPAC Resources'''], [http://www.openbiosystems.com/Genomics/Model Organism Resources/Fly Resources/Drosophila Gene Collection (DG/  '''Open Biosystems'''], [http://www.geneservice.co.uk/products/cdna/DGC.jsp '''Geneservice'''], or the [http://dgrc.cgb.indiana.edu/ '''DGRC''']<br />for clones in [http://www.fruitfly.org/DGC/DGC.txt DGCr1.0] or [http://www.fruitfly.org/DGC/dgc2.txt DGCr2.0].
* <span class="bold">Drosophila Genomics Resource Center (DGRC)</span><br />Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at http://dgrc.cgb.indiana.edu/
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* '''Drosophila Genomics Resource Center (DGRC)'''<br />Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at http://dgrc.cgb.indiana.edu/
* <span class="bold">EST Project cDNA Clones</span><br />Contact the [http://dgrc.cgb.indiana.edu/ <span class="bold">DGRC</span>] for cDNA clones from the BDGP EST collection.
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* '''EST Project cDNA Clones'''<br />Contact the [http://dgrc.cgb.indiana.edu/ '''DGRC'''] for cDNA clones from the BDGP EST collection.
  
 
=Arrays and Primers=
 
=Arrays and Primers=

Revision as of 15:43, 17 April 2014

Genomic Clones

BAC Genomic clones and Filters

  • One BAC clone library made from DNA of the same y1 ; cn1 bw1 sp1 stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
  • BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
  • Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
  • For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org

P1 Genomic Clones

  • As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
  • For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.

Cosmid Genomic Clones and Filters

The members of the European Drosophila Genome mapping consortium are:

  • F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
    Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
  • D.M. Glover and R.D.C. Saunders,
    Department of Biochemistry, University of Dundee, Dundee, Scotland.
  • M. Ashburner,
    Department of Genetics, University of Cambridge, Cambridge, England.
  • J. Modolell,
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
    with initial funding from the Fondation Schlumberger.

Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.

Contact:

Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm

cDNA (EST Clones and Libraries

Arrays and Primers

Stocks

Expression Vectors

Cell Lines and Hybridomas

Antibodies

RNA

RNAi

Transgenic, Targeted Mutation, and Other Services