Difference between revisions of "FlyBase:Drosophila Material Resources"

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* '''Bloomington Stock Center'''<br />Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at [http://flystocks.bio.indiana.edu/ http://flystocks.bio.indiana.edu]
* '''DrosDel Project'''<br />Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at http://www.drosdel.org.uk/
* '''Drosophila Gene Search Project'''<br />Information about ordering P{GS} lines can be found at http://kyotofly.kit.jp/stocks/documents/GS_lines.html
* '''Ehime Drosophila Species Stock Center of Japan'''<br />A collection of wild-type strains of ''D. melanogaster'' and strains of some 30 species can be found at http://kyotofly.kit.ac.jp/ehime/
* '''Exelixis Collection at Harvard Medical School'''<br />Information about ordering the Exelixis stocks at Harvard Medical School can be found at http://drosophila.med.harvard.edu/
* '''FlyORF'''<br />A collection of well-characterized transgenic ''D. melanogaster'' UAS-ORF lines produced and distributed by the Zurich ORFeome Project. http://flyorf.ch/
* '''FlyTrap Stock Collection'''<br />GFP protein trap lines from the FlyTrap project can be obtained at Yale University, USA at http://flytrap.med.yale.edu/
* '''FlyView Stock Collection'''<br />Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at http://flyview.uni-muenster.de/
* '''Gene Disruption Project (GDP)'''<br />Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at http://flypush.imgen.bcm.tmc.edu/pscreen/
* '''Kyorin-Fly'''<br />A collection of mutant and wildtype stocks of Drosophila species, especially the ''Drosophila ananassae'' species subgroup, ''D. auraria'' complex, and ''D. hydei''. http://kyotofly.kit.jp/cgi-bin/kyorin/index.cgi
* '''Kyoto Stock Center'''<br />Information about stocks at the Drosophila Genetic Resource Center (DGRC, Japan) at the Kyoto Institute of Technology, Japan can be found at http://www.dgrc.kit.ac.jp/en/index.html
* '''NIG-FLY: Fly Stocks of the National Institute of Genetics'''<br />Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at http://www.shigen.nig.ac.jp/fly/nigfly/
* '''Tübingen Drosophila Stock Collection'''<br />A [http://www.eb.tuebingen.mpg.de/departments/3-genetics/drosophila/drosophila-stock-collection/drosophila-stock-collection?set_language=en&cl=en list of approximately 2,000 Drosophila stocks] at the Max-Planck-Institute for Developmental Biology.
* '''UC San Diego Drosophila Stock Center'''<br />Approximately 270 different Drosophila species at University of San Diego, USA can be obtained at https://stockcenter.ucsd.edu/info/welcome.php
* '''University of Cambridge FlyProt project'''<br /> Annotations, expression patterns and sequence data for the YFP protein trap lines from the FlyProt project can be viewed on the Flannotator at http://www.flyprot.org. Stocks are available from the [http://kyotofly.kit.jp/cgi-bin/stocks/index.cgi Kyoto Stock Center.]
* '''Vienna Drosophila RNAi Center'''<br /> The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic <span class="italic">Drosophila''' RNAi libraries, at http://stockcenter.vdrc.at/control/main
=Expression Vectors=
=Expression Vectors=
=Cell Lines and Hybridomas=
=Cell Lines and Hybridomas=

Revision as of 15:46, 17 April 2014

Genomic Clones

BAC Genomic clones and Filters

  • One BAC clone library made from DNA of the same y1 ; cn1 bw1 sp1 stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
  • BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
  • Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
  • For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org

P1 Genomic Clones

  • As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
  • For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.

Cosmid Genomic Clones and Filters

The members of the European Drosophila Genome mapping consortium are:

  • F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
    Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
  • D.M. Glover and R.D.C. Saunders,
    Department of Biochemistry, University of Dundee, Dundee, Scotland.
  • M. Ashburner,
    Department of Genetics, University of Cambridge, Cambridge, England.
  • J. Modolell,
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
    with initial funding from the Fondation Schlumberger.

Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.


Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm

cDNA (EST Clones and Libraries

Arrays and Primers

  • Affymetrix
    The Affymetrix GeneChip Drosophila Genome Array for gene expression analysis based on Release 3.1 annotations can be obtained at http://www.affymetrix.com/products/arrays/specific/fly_2.affx

    The Affymetrix Drosophila Tiling 2.0R Array based on Genome Release 5 with comprehensive genome coverage at 38bp resolution can be obtained here: http://www.affymetrix.com/products/arrays/specific/drosophila_tiling2_0r.affx
  • Drosophila Genomics Resource Center (DGRC)
    Produces and distributes DNA microarray slides. More information is available at http://dgrc.cgb.indiana.edu/
  • FlyChip
    FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/
  • FlyPrimerBank
    An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at www.flyrnai.org/flyprimerbank
  • International Drosophila Array Consortium (INDAC)
    An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at http://www.indac.net/
  • UK Drosophila Affymetrix Microarray Service
    A publicly funded microarray service for the UK Drosophila research community. More information is available at http://www.mblab.gla.ac.uk/igf/


Expression Vectors

Cell Lines and Hybridomas




=Transgenic, Targeted Mutation, and Other Services=