Difference between revisions of "FlyBase:Antibodies"

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=Popular Resource Categories=
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= Popular Resource Categories =
  
 
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|style="text-align: center; padding: 15px;"| <big>[[FlyBase:External_Resources|All Resources]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:CRISPR|CRISPR]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Stocks|Stocks]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:RNAi|RNAi]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Model_Organism_Databases|Model Organism<br/> Databases]]</big>
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|style="text-align: center; padding: 15px;"| <big>[[FlyBase:External_Resources|All Resources]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:CRISPR|CRISPR]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:ScRNA-Seq|ScRNA-Seq]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:RNAi|RNAi]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Stocks|Stocks]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase: Antibodies|Antibodies]]</big>||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Neuroscience|Neuroscience]]</big>
 
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|style="text-align: center; padding: 20px;"| <big>[[FlyBase:Neuroscience|Neuroscience]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase: Antibodies|Antibodies]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Images|Images]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Maps|Maps]]</big> ||style="text-align: center; padding: 20px;"| <big>[http://www.flyrnai.org/tools/protocols/web/ Protocols]</big>
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{|class="wikitable"
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|style="text-align: center; padding: 20px;"| <big>[[FlyBase:Model_Organism_Databases|Model Organism<br/> Databases]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Images|Images]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Maps|Maps]]</big> ||style="text-align: center; padding: 20px;"| <big>[http://www.flyrnai.org/tools/protocols/web/ Protocols]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:Papers_with_technical_advances|Papers with<br/> Technical Advances]]</big> ||style="text-align: center; padding: 20px;"| <big>[[FlyBase:GSEA|Gene Set<br/> Enrichment Tools]]</big>
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__TOC__
 
__TOC__
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=Review antibodies tested in <em>Drosophila</em>.=
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[[File:Pabmabs-logo.png|600px|center]]
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pAbmAbs, a self-described Tripadvisor for antibodies, is a nonprofit resource to help researchers identify antibodies that work well, or do not work well, for their particular application by cataloguing both positive and negative community-submitted antibody reviews. FlyBase is working with pAbmAbs in an effort to increase the number of reviews submitted by ''Drosophila'' researchers and thereby increase pAbmAbs’ usefulness to the fly community. Submitting antibody reviews is easy and takes only a few minutes; we’ve even compiled some guidelines for you below. For your convenience, you may fill in this [[:File:Review sheet.xlsx|spreadsheet]] and email it to [mailto:molgaard@pabmabs.com Simon at pAbmAbs] for 'concierge' entry into the pAbmAbs database. Alternatively, you may go to the [http://pabmabs.com/ pAbmAbs website] and enter antibody reviews individually yourself.
 +
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'''Suggested details to include in antibody reviews:'''
 +
:*Antibody name
 +
:*Source/Vendor
 +
:*Catalog number
 +
:*Original antigen
 +
:*Drosophila target/ortholog
 +
:*Assay
 +
:*Tissue/Cell line
 +
:*Antibody dilution/concentration
 +
:*Important experimental details (e.g. fixation conditions)
 +
:*Reference, if published
 +
 +
Please note that all submitted reviews are screened by pAbmAbs so there may be a delay between submission and appearance on the website.
 +
 +
=Metasearches for antibodies.=
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{|cellpadding=5
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|-
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!style="background: #efefef;"| Resource
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|-
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|style="background: #efefef;"| [https://www.biocompare.com/Antibodies/ BioCompare Antibody Search Tool]
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|-
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|style="background: #efefef;"| [https://landing.benchsci.com/academic BenchSci AI Literature Screen for Antibodies]
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|}
  
 
=Links to collections of <em>Drosophila</em>-specific antibodies.=
 
=Links to collections of <em>Drosophila</em>-specific antibodies.=
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|style="background: #efefef;"| [http://www.abbiotec.com/antibodies?populate=&field_antibody_subcategory_tid=All&field_isotype_value=All&field_species_reactivity_value=Ds&field_applications_value=All&field_protein_family_tid=All Abbiotec] ||style="background: #efefef;"|San Diego, CA, USA
 
|style="background: #efefef;"| [http://www.abbiotec.com/antibodies?populate=&field_antibody_subcategory_tid=All&field_isotype_value=All&field_species_reactivity_value=Ds&field_applications_value=All&field_protein_family_tid=All Abbiotec] ||style="background: #efefef;"|San Diego, CA, USA
 
|-
 
|-
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|[http://www.abcam.com/products?keywords=drosophila&selected.classification=Primary+antibodies&selected.reactivity=Fruit+fly+(Drosophila+melanogaster) Abcam] ||Cambridge, MA, USA
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|[http://www.abcam.com/products?selected.classification=Primary+antibodies&selected.immunogenSpecies=Drosophila+melanogaster Abcam] ||Cambridge, MA, USA
 
|-
 
|-
 
|style="background: #efefef;"|[https://us.acris-antibodies.com/antibodies/primary-antibodies.htm?ab_ag_reactivity=Dros Acris Antibodies] ||style="background: #efefef;"|San Diego, CA, USA<br/> Herford, Germany
 
|style="background: #efefef;"|[https://us.acris-antibodies.com/antibodies/primary-antibodies.htm?ab_ag_reactivity=Dros Acris Antibodies] ||style="background: #efefef;"|San Diego, CA, USA<br/> Herford, Germany
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|style="background: #efefef;"|[http://www.antibodypedia.com/explore/drosophila Antibodypedia] ||style="background: #efefef;"| Vancouver, BC, Canada<br/>
 
|style="background: #efefef;"|[http://www.antibodypedia.com/explore/drosophila Antibodypedia] ||style="background: #efefef;"| Vancouver, BC, Canada<br/>
 
|-
 
|-
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|[http://antibodyregistry.org/search?q=Drosophila Antibody Registry] || Park Laboratory<br/> Harvard Medical School<br/> Boston, MA, USA
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|[http://antibodyregistry.org/search?q=Drosophila Antibody Registry] || RINL Resource Identification Initiative<br/>
 
|-
 
|-
 
|style="background: #efefef;"|[http://compbio.med.harvard.edu/antibodies/species/1 Antibody Validation Database] ||style="background: #efefef;"| University of California San Diego<br/> San Diego, CA, USA
 
|style="background: #efefef;"|[http://compbio.med.harvard.edu/antibodies/species/1 Antibody Validation Database] ||style="background: #efefef;"| University of California San Diego<br/> San Diego, CA, USA
 
|-
 
|-
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|[http://www.biorbyt.com/products/antibodies?speciesselect=51 Biorbyt] || Cambridge, UK<br/>  
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|[https://www.biorbyt.com/solr/index/?q=Drosophila Biorbyt] || Cambridge, UK<br/>  
 
|-
 
|-
 
|style="background: #efefef;"|[http://www.cellsignal.com/browse/d-melanogaster?N=4294956287+4294964832+4294960387 Cell Signaling Technology] ||style="background: #efefef;"| Danvers, MA, USA<br/> International Locations
 
|style="background: #efefef;"|[http://www.cellsignal.com/browse/d-melanogaster?N=4294956287+4294964832+4294960387 Cell Signaling Technology] ||style="background: #efefef;"| Danvers, MA, USA<br/> International Locations
 
|-
 
|-
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| [http://dshb.biology.uiowa.edu/Antibody-Collections/Drosophila-antigens Developmental Studies Hybridoma Bank (DSHB)] || University of Iowa<br/> Iowa City, IA, USA
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| [https://modelorg-ab.creative-biolabs.com/category-d-melanogaster-25.htm Creative Biolabs Modelorg Antibody]|| Shirley, NY, USA
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|-
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|style="background: #efefef;"|[http://dshb.biology.uiowa.edu/Antibody-Collections/Drosophila-antigens Developmental Studies Hybridoma Bank (DSHB)] || style="background: #efefef;"| University of Iowa<br/> Iowa City, IA, USA
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|-
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| [http://fabgennix.com/epages/862cc93a-7f43-4d98-8516-1e11a7720fd8.sf/en_US/?ObjectID=81707&ViewAction=ViewFaceted&FacetValue_CategoryID=81707&FacetValue_PreDefString_en_1540487778=D.+melanogaster&CurrencyID=USD&CurrencyID=USD&FacetRange_ListPrice=&FacetRange_ListPrice= FabGennix] || Frisco, TX, USA
 +
|-
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|style="background: #efefef;"|[https://www.genetex.com/Product?category=0&keyword=drosophila&type=01&page=1 GeneTex] || style="background: #efefef;"|Irvine, CA, USA<br/>International Locations
 +
|-
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| [http://www.emdmillipore.com/US/en/search/drosophila?search=&TrackingSearchType=SB+-+Search+Box&SearchContextPageletUUID=&SearchTerm=drosophila MilliporeSigma]|| International Locations
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|-
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|style="background: #efefef;"| [http://www.novusbio.com/drosophilaantibodies.html Novus]||style="background: #efefef;"| Littleton, CO, USA
 
|-
 
|-
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|style="background: #efefef;"|[http://fabgennix.com/epages/862cc93a-7f43-4d98-8516-1e11a7720fd8.sf/en_US/?ObjectID=81707&ViewAction=ViewFaceted&FacetValue_CategoryID=81707&FacetValue_PreDefString_en_1540487778=D.+melanogaster&CurrencyID=USD&CurrencyID=USD&FacetRange_ListPrice=&FacetRange_ListPrice= FabGennix] ||style="background: #efefef;"| Frisco, TX, USA
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| [https://www.prosci-inc.com/products/?s=Drosophila&filter_species-reactivity=drosophila ProSci] || El Cajon, CA, USA
 
|-
 
|-
−
| [http://www.genetex.com/Web/search/Catalog.aspx?Type=1&Category=494&Reactivity=Drosophila&Page=1 GeneTex] || Irvine, CA, USA<br/>International Locations
+
|style="background: #efefef;"| [https://www.rndsystems.com/search?keywords=primary%20antibodies&species=Drosophila R&D Systems]||style="background: #efefef;"|  Minneapolis, MN, USA
 
|-
 
|-
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|style="background: #efefef;"|[http://www.novusbio.com/drosophilaantibodies.html Novus]||style="background: #efefef;"| Littleton, CO, USA
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| [https://www.rndsystems.com/search?keywords=primary%20antibodies&species=Drosophila R&D Systems]|| Minneapolis, MN, USA
 
|-
 
|-
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| [http://www.prosci-inc.com/primary-antibodies.html?species_reactivity=601 ProSci] || Poway, CA, USA
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|style="background: #efefef;"| [http://www.scbt.com/section/primary_antibodies_non-mammalian/drosophila_proteins.html Santa Cruz Biotechnology]||style="background: #efefef;"| Dallas, Texas, USA
 
|-
 
|-
−
|style="background: #efefef;"| [https://www.rndsystems.com/search?keywords=primary%20antibodies&species=Drosophila R&D Systems]||style="background: #efefef;"| Minneapolis, MN, USA
+
| [https://www.sigmaaldrich.com/US/en/search/antibodies?facet=facet_web_species_reactivity%3A%3CI%3EDrosophila%3C%2FI%3E&focus=products&page=1&perpage=30&sort=relevance&term=antibodies&type=product Sigma Aldrich] ([http://www.sigmaaldrich.com/catalog/search?interface=All&N=0+14574647+4294666151&mode=partialmax&focus=product&lang=en&region=US additional link])|| St. Louis, MO, USA <br/> International Locations
 
|-
 
|-
−
| [http://www.scbt.com/section/primary_antibodies_non-mammalian/drosophila_proteins.html Santa Cruz Biotechnology]|| Dallas, Texas, USA
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|style="background: #efefef;"|[https://www.thermofisher.com/antibody/primary/query/Drosophila Thermo Fisher] || style="background: #efefef;"|Waltham, MA, USA
 
|-
 
|-
−
|style="background: #efefef;"| [http://www.sigmaaldrich.com/catalog/search?interface=All&N=0+14574647+4294666762&mode=partialmax&focus=product&lang=en&region=US Sigma Aldrich] ([http://www.sigmaaldrich.com/catalog/search?interface=All&N=0+14574647+4294666151&mode=partialmax&focus=product&lang=en&region=US additional link])||style="background: #efefef;"| St. Louis, MO, USA <br/> International Locations
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| [https://www.usbio.net/search/antibodies/?q=drosophila US Biological] || International Locations
 
|}
 
|}
  
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!style="background: #efefef;"| Resource !!style="background: #efefef;"| Author/Source
 
!style="background: #efefef;"| Resource !!style="background: #efefef;"| Author/Source
 
|-
 
|-
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|style="background: #efefef;"| [http://www.abgent.com/ Abgent] ||style="background: #efefef; whitespace:no-wrap;"|San Diego, CA, USA <br/> Suzhou city, Jiangsu Province, China
+
|style="background: #efefef;"| [https://www.abcepta.com/news-announcements/Abgent-Antibody-Custom-Services-News Abgent] ||style="background: #efefef; whitespace:no-wrap;"|San Diego, CA, USA <br/> Suzhou city, Jiangsu Province, China
 
|-
 
|-
 
|[http://www.antibodies-online.com/ antibodies-online] ||International Locations
 
|[http://www.antibodies-online.com/ antibodies-online] ||International Locations
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|[https://www.lsbio.com/ LifeSpan BioSciences] ||Seattle, WA, USA
 
|[https://www.lsbio.com/ LifeSpan BioSciences] ||Seattle, WA, USA
 
|-
 
|-
−
|style="background: #efefef;"|[https://www.thermofisher.com/us/en/home.html Thermo Fisher] || style="background: #efefef;"|Waltham, MA, USA
 
 
|}
 
|}
  

Latest revision as of 21:19, 11 October 2023

Popular Resource Categories

All Resources CRISPR ScRNA-Seq RNAi Stocks Antibodies Neuroscience
Model Organism
Databases		 Images Maps Protocols Papers with
Technical Advances		 Gene Set
Enrichment Tools

Review antibodies tested in Drosophila.

Pabmabs-logo.png

pAbmAbs, a self-described Tripadvisor for antibodies, is a nonprofit resource to help researchers identify antibodies that work well, or do not work well, for their particular application by cataloguing both positive and negative community-submitted antibody reviews. FlyBase is working with pAbmAbs in an effort to increase the number of reviews submitted by Drosophila researchers and thereby increase pAbmAbs’ usefulness to the fly community. Submitting antibody reviews is easy and takes only a few minutes; we’ve even compiled some guidelines for you below. For your convenience, you may fill in this spreadsheet and email it to Simon at pAbmAbs for 'concierge' entry into the pAbmAbs database. Alternatively, you may go to the pAbmAbs website and enter antibody reviews individually yourself.

Suggested details to include in antibody reviews:

  • Antibody name
  • Source/Vendor
  • Catalog number
  • Original antigen
  • Drosophila target/ortholog
  • Assay
  • Tissue/Cell line
  • Antibody dilution/concentration
  • Important experimental details (e.g. fixation conditions)
  • Reference, if published

Please note that all submitted reviews are screened by pAbmAbs so there may be a delay between submission and appearance on the website.

Metasearches for antibodies.

Resource
BioCompare Antibody Search Tool
BenchSci AI Literature Screen for Antibodies

Links to collections of Drosophila-specific antibodies.

Resource Author/Source
Abbiotec San Diego, CA, USA
Abcam Cambridge, MA, USA
Acris Antibodies San Diego, CA, USA
Herford, Germany
Active Motif Carlsbad, CA, USA
International Locations
Antibodypedia Vancouver, BC, Canada
Antibody Registry RINL Resource Identification Initiative
Antibody Validation Database University of California San Diego
San Diego, CA, USA
Biorbyt Cambridge, UK
Cell Signaling Technology Danvers, MA, USA
International Locations
Creative Biolabs Modelorg Antibody Shirley, NY, USA
Developmental Studies Hybridoma Bank (DSHB) University of Iowa
Iowa City, IA, USA
FabGennix Frisco, TX, USA
GeneTex Irvine, CA, USA
International Locations
MilliporeSigma International Locations
Novus Littleton, CO, USA
ProSci El Cajon, CA, USA
R&D Systems Minneapolis, MN, USA
R&D Systems Minneapolis, MN, USA
Santa Cruz Biotechnology Dallas, Texas, USA
Sigma Aldrich (additional link) St. Louis, MO, USA
International Locations
Thermo Fisher Waltham, MA, USA
US Biological International Locations


Links to vendors whose collections include Drosophila-specific antibodies.

The table below contains non-direct Drosophila antibody links; additional searching is required from the vendor website.

Resource Author/Source
Abgent San Diego, CA, USA
Suzhou city, Jiangsu Province, China
antibodies-online International Locations
Enzo Life Sciences Farmingdale, NY, USA
LifeSpan BioSciences Seattle, WA, USA


Lab-generated antibodies in Gene Reports

Antibodies generated by research laboratories for specific proteins are listed in their respective Gene Report under "Stocks and Reagents" → "Antibody Information".
For example, the following screenshot is taken from the Notch gene report:

Antibodies.png

If no antibodies exist or they have not yet been curated, this section will be empty.


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