FlyBase:ID Validator
Overview
You can use the ID Converter tool to:
(i) validate a set of symbols/IDs, which will update any old symbols/IDs to their current equivalents (where possible); or
(ii) validate and convert a set of symbols/IDs, which will additionally convert the submitted list from one data class to another (where feasible), such as converting a list of allele or transcript IDs to their corresponding gene IDs OR convert certain external IDs (GenBank, UniProt, PubMed) into FlyBase IDs.
(iii) to simply upload ID lists into a HitList for further analysis/processing within FlyBase.
Usage
1. Either type/paste in a set of IDs/symbols into the 'Enter IDs or Symbols:' box, or choose to Upload an file of IDs by clicking the Browse button. Spaces or returns should be used to separate the IDs/symbols (no commas or other text separators). The supported input types include:
- FlyBase IDs (for most data classes)
- FlyBase symbols (for most data classes)
- FlyBase gene annotation symbols (CG#)
- clone names
- PubMed IDs
- GenBank nucleotide/protein accessions
- Uniprot (Swiss-Prot/TrEMBL) accessions
2. Choose to 'Validate Only' or 'Validate and Convert', choosing the desired conversion data class from the drop-down menu. (Note that IDs/symbols pertaining to different data classes (e.g. gene and alleles) may be submitted if choosing to 'Validate Only', but will results in conversion errors if chossing to 'Validate and Convert'.) The available 'convert to' options are:
- Genes
- Alleles
- Aberrations
- Balancers
- Transgenic constructs
- Natural transposons
- Insertions
- Transcripts
- Polypeptides
- Clones
- References
Note that only a subset of all possible conversions make sense - attempting to make non-sensical conversions (e.g. transcripts -> alleles) will result in a blank output table. A table showing common/useful conversion types is shown below.
3. Click on the 'Submit Query' button.
4. The resulting table has three sections:
i) A header line listing the number of:
- Submitted IDs
- Validated/Updated IDs
- Unknown IDs
- Unique converted IDs
ii) Buttons to export/download the final list of converted IDs to:
- a FlyBase HitList
- a local file of unique FB IDs only
- a local file of the conversion table in TSV format
iii) The conversion table, comprising 4 columns showing the:
- Submitted symbol/ID
- Current FlyBase ID
- Converted FlyBase ID
- The FlyBase symbol, hyperlinked to the relevant FlyBase record
The table is color-coded:
- current FlyBase IDs are colored green
- other recognized (i.e. updateable/convertible) IDs/symbols are colored yellow
- unknown (ie. unconvertible) IDs/symbols are colored red
| Input (example) | Output (example) | Logic |
|---|---|---|
| Genes (FBgn ID or FlyBase symbol) | Polypeptides (FBpp ID and symbol) | List all polypeptides corresponding to each gene |
| Genes (FBgn ID or FlyBase symbol) | Alleles (FBal ID and symbol) | List all alleles corresponding to each gene |
| Alleles (FBal ID or FlyBase symbol) | Genes (FBgn ID and FlyBase symbol) | List all genes corresponding to each allele |
| Genes (FBgn ID or FlyBase symbol) | References (FBrf ID) | List all references associated with each gene |
| References (FBrf ID or PMID) | Genes (FBgn ID and FlyBase symbol) | List all genes associated with each reference |
| References (PMID) | References (FBrf ID) | Convert PubMed IDs (PMIDs) to FlyBase reference (FBrf) IDs |
| Genes (GenBank nucleotide accession) | Genes (FBgn ID and FlyBase symbol) | Convert GenBank nucleotide accessions to FlyBase gene IDs |
| Proteins (UniProt or GenBank Protein accession) | Genes (FBgn ID and FlyBase symbol) | Convert external protein accessions to FlyBase gene IDs |