FlyBase:Vectors and Constructs
Revision as of 06:47, 15 May 2015 by Lauraponting (talk | contribs)
Genomic Insertion Collections
Gene Disruption Project Constructs
| P{PZ} | lacZ enhancer trap |
| P{lacW} | lacZ enhancer trap |
| P{GT1} | gene trap |
| P{SUPor-P} | |
| P{EP} | mobile activating element |
| P{EPg} | mobile activating element |
| P{EPgy2} | mobile activating element |
| P{Mae-UAS.6.11} | mobile activating element |
| PBac{PB} | (Exelixis) |
| PBac{GAL4D,EYFP} | GAL4 binary enhancer trap |
| Mi{ET1} | GAL4 binary enhancer trap |
| Mi{MIC} | GFP gene trap, φC31 target |
Drosophila Gene Search Project Constructs
| P{GSV1} | mobile activating element |
| P{GSV2} | mobile activating element |
| P{GSV3} | mobile activating element |
| P{GSV6} | mobile activating element |
| P{GSV7} | mobile activating element |
Deletion Generators
| P{XP} | recombination |
| PBac{RB} | recombination |
| PBac{WH} | recombination |
| P{RS5} | recombination |
| P{RS3} | recombination |
| PBac{5HPw[+]} | recombination |
| PBac{3HPy[+]} | recombination |
| P{wHy} | hobo mobilization |
Protein Trap Constructs
| P{PTT-GA} | GFP gene trap |
| P{PTT-GB} | GFP gene trap |
| P{PTT-GC} | GFP gene trap |
Constructs Used for Other Collections
| P{A92} | lacZ enhancer trap |
| P{lArB} | lacZ enhancer trap |
| P{lwB} | lacZ enhancer trap |
| P{hsneo} | |
| P{ry11} | |
| P{GawB} | GAL4 binary enhancer trap |
| PBac{SAstopDsRed} | Tni\piggyBac mutator |
| pP{MF3} | VDRC-1 dsRNA construct collection vector |
| pVALIUM10 | TRiP-1 dsRNA construct collection vector |
Genome Project Cloning Vectors
cDNA Cloning Vectors
| pOT2 | |
| pOTB7 | |
| pCDNA-SK[+] | |
| pCR2.1 | |
| pFLC-I | |
| pSport1-Tag21 |
BAC Vectors for Large Genomic Clones
| pBACe3.6 |
Basic Plasmid Cloning Vectors for P Transformation
Carnegie 20 Derivatives
| pP{Car20} | ry+t7.2 |
| pP{Car20.1} | ry+t7.2 |
| pP{DM30} | ry+t7.2 |
CaSpeR Derivatives
| pP{CaSpeR} | w+mC |
| pP{CaSpeR-2} | w+mC |
| pP{CaSpeR-3} | w+mC |
| pP{CaSpeR-4} | w+mC |
| pP{CaSpeR-5} | w+mC |
Others
| pP{hsneo} | Tn\neoRHsp70Bb.PS |
| pP{YES} | y+mDint2 |
| pP{W8} | w+mW.hs |
| pP{YC1.8} | v+t1.8 |
| pP{Car4} | -none- |
Plasmid Cloning Vectors with a Promoter
| pP{CaSpeR-hs/act} | Hsp70 |
| pP{CaSpeR-hs} | Hsp70 |
| pP{CaSpeR-Hsp70/SV40} | Hsp70 |
| pP{Hsp70-CaSpeR} | Hsp70 |
| pP{W8H} | Hsp70 |
| pP{CaSpeR-act(B)} | Act5C |
| pP{CaSpeR-act(R)} | Act5C |
| pP{hsneo-act(B)} | Act5C |
| pP{hsneo-act(R)} | Act5C |
| pP{GMR} | GMR (eye-specific) |
| pP{COG} | otu (oogenesis) |
Plasmid Cloning Vectors with a Reporter Gene
| pP{Pelican} | LacZ |
| pP{H-Pelican} | LacZ |
| pP{GreenPelican} | GFP (vital reporter) |
| pP{GreenH-Pelican} | GFP (vital reporter) |
| pP{Stinger} | GFP (vital reporter) |
| pP{H-Stinger} | GFP (vital reporter) |
| pP{RedH-Stinger} | RFP (vital reporter) |
| pP{RedH-Pelican} | RFP (vital reporter) |
| pP{YellowH-Pelican} | YFP (vital reporter) |
| pP{CaSpeR-lacZ} | LacZ |
| pP{CaSpeR-betagal} | LacZ |
| pP{CaSpeR-AUG-betagal} | LacZ |
| pP{DM79} | LacZ |
| pP{DM26} | CAT |
| pP{HZ50} | LacZ |
| pP{HZ50PL} | LacZ |
| pP{HZR} | LacZ |
Transposase Sources
| pπ25.7wc | P transposase |
| pP{Δ2-3} | P transposase |
| pUChsΔ2-3 | P transposase |
| H{PΔ2-3} | P transposase |
| PBac{Δ2-3.Exel} | P transposase |
| pP{HBL1} | hobo transposase |
| pP{hsH\T-2} | hobo transposase |
| P{hsILMiT} | Dhyd\Minos transposase |
| P{Hsp70-PBac\T} | Tni\piggyBac transposase |
| P{Tub-PBac\T} | Tni\piggyBac transposase |
Cell Ablation Vectors and Constructs
| pP{RMC4} | |
| pP{UAS-RAcs2} | |
| P{UAS-hid} | |
| P{UAS-hid.C} | |
| P{hs-hid} | |
| P{UAS-Hsap\KCNJ2.EGFP} | |
| P{UAS-NaChBac} | |
| P{UAS-Cbβ\DT-A.I} | |
| P{GMR-rpr.H} | |
| P{UAS-rpr.C} | |
| P{GMR-grim} |
FLP/FRT Constructs
| pP{hsFLP} | |
| P{70FLP} | |
| P{hsp70-flp} | |
| pP{neoFRT} | |
| P{FRT(w+t1.8)} | |
| pP{lacZ.FRT.B} | |
| P{lacZ.Act5C.B} | |
| pP{wg.FRT.S} | |
| pJ35 | |
| P{ey-FLP.N} | |
| P{ey3.5-FLP.Exel} | |
| P{ovo-FLP.R} |
GAL4/UAS Cloning Vectors
| pP{UAST} | |
| pP{UASp} | |
| pP{UAS-C5} | |
| pP{GawB} | |
| pP{WIZ} | RNAi, UAS |
| pP{PTGAL} | |
| P{GD264} | |
| pGaTN | |
| pGaTB |
GAL4/UAS Target Constructs
| P{UAS-lacZ.B} | |
| P{UAS-lacZ.Hsp70} | |
| P{UAS-lacZ.N} | |
| P{UAS-tau-lacZ.B} | |
| P{UAS-lacZ.NUC} | |
| P{UAS-GFP.S65T} | |
| P{UAS-RAcs2} | Cell ablation |
| P{AyGAL4} | Mosaic analyses |
Cell Culture Vectors
| pMT-GAL4 | |
| pRmHa-1 | |
| pRmHa-3 | |
| pAct5CSRS | |
| pP-Ac | |
| pPacPL | |
| pBLCAT3 | |
| pP{C4cat} | |
| pSCAT0 | |
| pHGCO | |
| p0-CAT | |
| pcopneo |
Targeted Replacement Vectors and Constructs
| pP{W25} | ends-out replacement |
| pP{W30} | ends-out replacement |
| pP{W35} | ends-out replacement |
| P{70I-SceI} | source of Scer\SCEI endonuclease |
| P{hsFLP} | source of Scer\FLP1 recombinase |
φC31 Recombination-mediated genomic insertion
Cloning vectors for donor element
| Pacman1-ApR | BAC cloning vector |
| Pacman1-CmR | BAC cloning vector |
| Pacman2-ApR | BAC cloning vector |
| Pacman2-CmR | BAC cloning vector |
| Pacman2-CmR-BW | BAC cloning vector |
| pUASTattB | plasmid cloning vector |
| pwP-Ex2UASTattB | plasmid cloning vector; split-white system |
Target elements
| PBac{y+-attP-3B} | target element |
| PBac{y+-attP-9A} | target element |
| M{wEx3-6.attP} | target element, split-white system |
| M{loxP.attP} | target element |
| M{3xP3-RFP.attP} | target element |
| M{3xP3-RFP.attP'} | target element |
| P{CaryP} | target element |
| P{UASTP2} | target element |
Integrase sources
| M{vas-int.B} | integrase source (source within genome) |
| M{vas-int.Dm} | integrase source (source within genome) |
| M{vas-int.NLS} | integrase source (source within genome) |
| M{nos-int.NLS} | integrase source (source within genome) |
| M{nos-int.B} | integrase source (source within genome) |
| pET11φC31poly(A) | template for integrase mRNA production |
Gateway recombinational cloning vectors (promoter; tag)
For a complete list of Gateway vectors please see the "Data from Reference" section of this page
| Vector | Promoter; Tag |
|---|---|
| pP{TWG} | UAS; EGFP |
| pP{TGW} | UAS; EGFP |
| pAGW | Act5C (cell culture); EGFP |
| pAWG | Act5C (cell culture); EGFP |
| pAHW | Act5C (cell culture); HA1 |
| pP{TMW} | UAS; Myc |
| pP{TWM} | UAS; Myc |
| pP{TWV} | UAS; YFP |
| pP{TVW} | UAS; YFP |
| pP{THW} | UAS; HA1 |
| pP{TWH} | UAS; HA1 |
| pP{TW} | UAS; [none] |
| pP{PWG} | UASp (female germline); EGFP |
| pP{PGW} | UASp (female germline); EGFP |