FlyBase:Drosophila Material Resources

Genomic Clones

BAC Genomic clones and Filters

• One BAC clone library made from DNA of the same y¹ ; cn¹ bw¹ sp¹ stock as was used for the Berkeley Drosophila Genome Project P1 clones is available. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
• BACR clones (18,432 clones in pBACe3.6; average clone size is 160-Kb) are available from BACPAC Resources at: http://bacpac.chori.org/dromel98.htm
• Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P(acman)-CmR-BW vector, a special adaptation of P[acman], are also available from BACPAC Resources see: http://pacmanfly.org/libraries.html
• For information and resources related to the P[acman] recombineering technique see: http://www.pacmanfly.org

P1 Genomic Clones

• As the P1 library is largely obsolete, the BDGP is discouraging the use of P1 clones. If there is a compelling scientific reason that a corresponding BAC is not sufficient, requests for P1 clones can be directed to the BDGP.
• For additional information about the Berkeley Drosophila Genome Project see http://www.fruitfly.org.

Cosmid Genomic Clones and Filters

• EDGP cosmids are available from the Geneservice:
  http://www.geneservice.co.uk/products/clones/drosophila.jsp.
• Cosmids are available either singly or on high-density filters.

The members of the European Drosophila Genome mapping consortium are:

• F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
  Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
• D.M. Glover and R.D.C. Saunders,
  Department of Biochemistry, University of Dundee, Dundee, Scotland.
• M. Ashburner,
  Department of Genetics, University of Cambridge, Cambridge, England.
• J. Modolell,
  Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain. Supported by the Stimulation Action Programme of the European Community,
  with initial funding from the Fondation Schlumberger.

Supported by the Stimulation Action Programme of the European Community, with initial funding from the Fondation Schlumberger.

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.

Contact:

Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources at: http://bacpac.chori.org/drosofosmid.htm

cDNA (EST Clones and Libraries

• Addgene
  Addgene helps labs archive and share their plasmids that have appeared in publications.
  http://www.addgene.org/pgvec1?f=c&cmd=browse
• Berkeley Drosophila Genome Project
  The information is provided by BDGP. If you don't find the information you need here check http://www.fruitfly.org/about/materials/ for possible updates.
• Brian Oliver Laboratory
  Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from the Geneservice in the UK.
  Ordering information can be found at http://www.geneservice.co.uk/products/cdna/dros_cdna.jsp
• cDNA library aliquots
  Aliquots from a number of the BDGP cDNA libraries are available from the labs on this list.
• Clontech
  Clontech (BD Biosciences) offers D. melanogaster embryo and larva 5'-STRETCH cDNA libraries at http://www.clontech.com/products/cat/HTML/1186.shtml
• Drosophila Gene Collection (DGC)
  Contact BACPAC Resources, Organism Resources/Fly Resources/Drosophila Gene Collection (DG/ Open Biosystems, Geneservice, or the DGRC
  for clones in DGCr1.0 or DGCr2.0.
• Drosophila Genomics Resource Center (DGRC)
  Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at http://dgrc.cgb.indiana.edu/
• EST Project cDNA Clones
  Contact the DGRC for cDNA clones from the BDGP EST collection.

Arrays and Primers

• Affymetrix
  The Affymetrix GeneChip Drosophila Genome Array for gene expression analysis based on Release 3.1 annotations can be obtained at http://www.affymetrix.com/products/arrays/specific/fly_2.affx
  
  The Affymetrix Drosophila Tiling 2.0R Array based on Genome Release 5 with comprehensive genome coverage at 38bp resolution can be obtained here: http://www.affymetrix.com/products/arrays/specific/drosophila_tiling2_0r.affx
• Drosophila Genomics Resource Center (DGRC)
  Produces and distributes DNA microarray slides. More information is available at http://dgrc.cgb.indiana.edu/
• FlyChip
  FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at http://www.flychip.org.uk/
• FlyPrimerBank
  An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at www.flyrnai.org/flyprimerbank
• International Drosophila Array Consortium (INDAC)
  An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at http://www.indac.net/
• UK Drosophila Affymetrix Microarray Service
  A publicly funded microarray service for the UK Drosophila research community. More information is available at http://www.mblab.gla.ac.uk/igf/

Stocks

• Bloomington Stock Center
  Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at http://flystocks.bio.indiana.edu
• DrosDel Project
  Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at http://www.drosdel.org.uk/
• Drosophila Gene Search Project
  Information about ordering P{GS} lines can be found at http://kyotofly.kit.jp/stocks/documents/GS_lines.html
• Ehime Drosophila Species Stock Center of Japan
  A collection of wild-type strains of D. melanogaster and strains of some 30 species can be found at http://kyotofly.kit.ac.jp/ehime/
• Exelixis Collection at Harvard Medical School
  Information about ordering the Exelixis stocks at Harvard Medical School can be found at http://drosophila.med.harvard.edu/
• FlyORF
  A collection of well-characterized transgenic D. melanogaster UAS-ORF lines produced and distributed by the Zurich ORFeome Project. http://flyorf.ch/
• FlyTrap Stock Collection
  GFP protein trap lines from the FlyTrap project can be obtained at Yale University, USA at http://flytrap.med.yale.edu/
• FlyView Stock Collection
  Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at http://flyview.uni-muenster.de/
• Gene Disruption Project (GDP)
  Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at http://flypush.imgen.bcm.tmc.edu/pscreen/
• Kyorin-Fly
  A collection of mutant and wildtype stocks of Drosophila species, especially the Drosophila ananassae species subgroup, D. auraria complex, and D. hydei. http://kyotofly.kit.jp/cgi-bin/kyorin/index.cgi
• Kyoto Stock Center
  Information about stocks at the Drosophila Genetic Resource Center (DGRC, Japan) at the Kyoto Institute of Technology, Japan can be found at http://www.dgrc.kit.ac.jp/en/index.html
• NIG-FLY: Fly Stocks of the National Institute of Genetics
  Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at http://www.shigen.nig.ac.jp/fly/nigfly/
• Tübingen Drosophila Stock Collection
  A list of approximately 2,000 Drosophila stocks at the Max-Planck-Institute for Developmental Biology.
• UC San Diego Drosophila Stock Center
  Approximately 270 different Drosophila species at University of San Diego, USA can be obtained at https://stockcenter.ucsd.edu/info/welcome.php
• University of Cambridge FlyProt project
  Annotations, expression patterns and sequence data for the YFP protein trap lines from the FlyProt project can be viewed on the Flannotator at http://www.flyprot.org. Stocks are available from the Kyoto Stock Center.
• Vienna Drosophila RNAi Center
  The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries, at http://stockcenter.vdrc.at/control/main

Expression Vectors

• Drosophila Gateway Vectors
  A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC
• ExpreS2ion Biotechnologies
  ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. ExpreS2ion Biotechnologies

Cell Lines and Hybridomas

• Drosophila Genomics Resource Center (DGRC)
  Collects and distributes cell lines. More information is available at http://dgrc.cgb.indiana.edu/
• Developmental Studies Hybridoma Bank
  The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at http://dshb.biology.uiowa.edu
• ATCC
  The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at http://www.atcc.org/

Antibodies

• Clontech
• Cocalico Biologicals
• Developmental Studies Hybridoma Bank
• Novagen
• Sigma-Aldrich
• US Biological

RNA

Clontech
Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2