Difference between revisions of "Resource Portal"
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=== Introduction === | === Introduction === | ||
| − | + | RNAi text to be added | |
| − | === RNAi Design | + | === RNAi Design/Analysis Tools === |
{|cellpadding=5 | {|cellpadding=5 | ||
|- | |- | ||
| − | !style="background: #efefef;"| Resource !!style="background: #efefef;"| | + | !style="background: #efefef;"| Resource !!style="background: #efefef;"| Description !!style="background: #efefef;"| Author/Source |
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| − | |style="background: #efefef; white-space | + | |style="background: #efefef; white-space"| [http://www.flyrnai.org/gess/ GESS]||style="background: #efefef; white-space: nowrap;"| Off-target RNAi prediction ||style="background: #efefef;"| DRSC, Harvard Medical School, Boston, MA, USA |
|- | |- | ||
| − | | [http://www. | + | |style="nowrap;"| [http://www.flyrnai.org/RNAi_find_frag_free.html Predicted Off-Target-Free Sequence Regions] || Enter sequences to find predicted off-target-free regions || DRSC, Harvard Medical School, Boston, MA, USA |
|- | |- | ||
| − | |style="background: #efefef;"| | + | |style="background: #efefef;"| [http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl SnapDragon]| ||style="background: #efefef;"| A tool to facilitate dsRNA design ||style="background: #efefef;"| DRSC, Harvard Medical School, Boston, MA, USA |
|- | |- | ||
| − | | | + | | [http://www.flyrnai.org/up-torr/ UP-TORR] || Updated Targets of RNAi Reagents || DRSC, Harvard Medical School, Boston, MA, USA |
|} | |} | ||
=== RNAi Stocks === | === RNAi Stocks === | ||
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{|cellpadding=5 | {|cellpadding=5 | ||
|- | |- | ||
| − | !style="background: #efefef;"| Resource !!style="background: #efefef;"| | + | !style="background: #efefef;"| Resource !!style="background: #efefef;"| Description !!style="background: #efefef;"| Author/Source |
|- | |- | ||
|style="background: #efefef; white-space: nowrap;"| [http://flystocks.bio.indiana.edu/Browse/misc-browse/CRISPR.htm CRISPR system stocks for site-specific mutagenesis]||style="background: #efefef; white-space: nowrap;"| Bloomington Stock Center ||style="background: #efefef;"| Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam et tincidunt ante. Donec tincidunt gravida velit at iaculis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean rutrum quam eget luctus condimentum. Cras in venenatis lectus, at laoreet augue. | |style="background: #efefef; white-space: nowrap;"| [http://flystocks.bio.indiana.edu/Browse/misc-browse/CRISPR.htm CRISPR system stocks for site-specific mutagenesis]||style="background: #efefef; white-space: nowrap;"| Bloomington Stock Center ||style="background: #efefef;"| Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam et tincidunt ante. Donec tincidunt gravida velit at iaculis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean rutrum quam eget luctus condimentum. Cras in venenatis lectus, at laoreet augue. | ||
Revision as of 16:56, 3 December 2015
**This page is a work-in-progress concept of reformatting our resource and reagent list.**
Popular Resources
| CRISPR | RNAi | Stock Centers | Antibodies | Other Example |
CRISPR
Introduction
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam vel vehicula risus. Ut id magna odio. Proin in bibendum odio, non dictum justo. Cras vitae eros arcu. Vestibulum porttitor nibh sit amet justo venenatis, vel tempor erat fringilla. Sed elementum varius ante. Donec eget neque mi. Vestibulum id nisi sit amet purus auctor porta eu quis elit. Nulla facilisi. Sed semper scelerisque ex, eu aliquet libero sollicitudin a.
gRNA Design Resources
| Resource | Description | Author/Source |
|---|---|---|
| DRSC Find CRISPR Tool | To be filled. | DRSC Harvard Medical School Boston, USA |
| CRISPR Optimal Target Finder | To be filled. | O'Connor-Giles, Wildonger, and Harrison Labs University of Wisconsin-Madison WI, USA |
| E-CRISPR | To be filled. | DKFZ/Boutros Lab Heildelberg, Germany |
| CHOPCHOP | To be filled. | Schier & Church Labs Harvard University Cambridge, USA |
| Genome Engineering | To be filled. | Zhang Lab MIT Boston, MA, USA |
| CRISPRscan | To be filled. | Giraldez Lab Yale University New Haven, USA |
| CRISPOR | To be filled. | Tefor France |
| CRISPR-ERA | To be filled. | Lei Stanley Qi Lab and Xiaowo Wang Lab Stanford University Standford, USA |
CRISPR Stocks
| Resource | Description | Author/Source |
|---|---|---|
| CRISPR system stocks for site-specific mutagenesis | Stocks used to express Cas9 nuclease and/or tracrRNA maternally so that chimeric RNAs (also referred to as guide RNAs) or CRISPR RNAs (crRNAs) can be injected into fly embryos to generate new mutations without the need for helper plasmids, or so that they can be used in combination with chimeric or CRISPR RNAs expressed from transgenic constructs. | Bloomington Stock Center Indiana University Indiana, USA |
| CRISPR Fly Design | To be filled. | MRC - Laboratory of Molecular Biology |
| The TRiP Toolbox Stocks | TRiP Toolbox stocks available at the BDSC. This Toolbox is the set of fly lines used to generate the RNAi transgenic lines, as well as GAL4 lines used by the TRiP that contain UAS-dicer2 to enhance the knock-down. | To be filled. |
CRISPR Vectors
| Resource | Author/Source | Additional Information |
|---|---|---|
| CRISPR system stocks for site-specific mutagenesis | Bloomington Stock Center | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam et tincidunt ante. Donec tincidunt gravida velit at iaculis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean rutrum quam eget luctus condimentum. Cras in venenatis lectus, at laoreet augue. |
| CRISPR Fly Design | MRC - Laboratory of Molecular Biology | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer id purus metus. Cras et lacus vel diam posuere facilisis. Vestibulum lorem metus, dapibus nec porta non, fringilla sit amet eros. |
| Example | Example | Example |
| Example | Example | Example |
CRISPR Methods Publications
Back to top. | Provide Feedback.
RNAi
Introduction
RNAi text to be added
RNAi Design/Analysis Tools
| Resource | Description | Author/Source |
|---|---|---|
| GESS | Off-target RNAi prediction | DRSC, Harvard Medical School, Boston, MA, USA |
| Predicted Off-Target-Free Sequence Regions | Enter sequences to find predicted off-target-free regions | DRSC, Harvard Medical School, Boston, MA, USA |
| SnapDragon| | A tool to facilitate dsRNA design | DRSC, Harvard Medical School, Boston, MA, USA |
| UP-TORR | Updated Targets of RNAi Reagents | DRSC, Harvard Medical School, Boston, MA, USA |
RNAi Stocks
| Resource | Description | Author/Source |
|---|---|---|
| CRISPR system stocks for site-specific mutagenesis | Bloomington Stock Center | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam et tincidunt ante. Donec tincidunt gravida velit at iaculis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean rutrum quam eget luctus condimentum. Cras in venenatis lectus, at laoreet augue. |
| CRISPR Fly Design | MRC - Laboratory of Molecular Biology | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer id purus metus. Cras et lacus vel diam posuere facilisis. Vestibulum lorem metus, dapibus nec porta non, fringilla sit amet eros. |
| Example | Example | Example |
| Example | Example | Example |
Back to top. | Provide Feedback.
Stock Centers
List of Drosophila Stock Centers
| Resource | Author/Source | Additional Information |
|---|---|---|
| CRISPR system stocks for site-specific mutagenesis | Bloomington Stock Center | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam et tincidunt ante. Donec tincidunt gravida velit at iaculis. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean rutrum quam eget luctus condimentum. Cras in venenatis lectus, at laoreet augue. |
| CRISPR Fly Design | MRC - Laboratory of Molecular Biology | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer id purus metus. Cras et lacus vel diam posuere facilisis. Vestibulum lorem metus, dapibus nec porta non, fringilla sit amet eros. |
| Example | Example | Example |
| Example | Example | Example |
Back to top. | Provide Feedback.
Additional Resources
- This section would contain links to additional resource pages or a copy+pasted version of the old resource page.