FlyBase:Drosophila Online Resources

Related Projects

GMOD

Gene Ontology (GO)

Reactome

BDGP (Berkeley Drosophila Genome Project)

DGRC (Drosophila Genomics Resource Center)

DIS by issue

FlyExpress

Interactive Fly

modENCODE

Textpresso for Fly

Stock Collections

D. melanogaster Collections

Bloomington

DrosDel

Exelixis (Harvard)

GDP (Baylor)

Kyoto

NIG-Fly

VDRC

Species Collections

Ehime

UC San Diego

Other public collections

Drosophila Gene Search Project

Information about ordering P{GS} lines can be found at http://gsdb.biol.metro-u.ac.jp/~dclust/index.html

Ehime Drosophila Species Stock Center of Japan

A collection of wild-type strains of D. melanogaster and strains of some 30 species can be found at http://kyotofly.kit.ac.jp/ehime/

FlyTrap Stock Collection

GFP protein trap lines from the FlyTrap project can be obtained at Yale University, USA at http://flytrap.med.yale.edu/

FlyView Stock Collection

Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at http://flyview.uni-muenster.de/

Kyorin-Fly

A collection of mutant and wildtype stocks of Drosophila species, especially the Drosophila ananassae species subgroup, D. auraria complex, and D. hydei. http://kyotofly.kit.jp/cgi-bin/kyorin/index.cgi

Tübingen Drosophila Stock Collection

A list of approximately 2,000 Drosophila stocks at the Max-Planck-Institute for Developmental Biology.

UC San Diego Drosophila Stock Center

Approximately 270 different Drosophila species at University of San Diego, USA can be obtained at https://stockcenter.ucsd.edu/info/welcome.php

University of Cambridge FlyProt project

Annotations, expression patterns and sequence data for the YFP protein trap lines from the FlyProt project can be viewed on the Flannotator at http://www.flyprot.org. Stocks are available from the Kyoto Stock Center.

Vectors and Constructs

Genomic Insertion Collections
Gene Disruption Project Constructs

P{PZ} lacZ enhancer trap P{lacW} lacZ enhancer trap P{GT1} gene trap P{SUPor-P} P{EP} mobile activating element P{EPg} mobile activating element P{EPgy2} mobile activating element P{Mae-UAS.6.11} mobile activating element PBac{PB} (Exelixis) PBac{GAL4D,EYFP} GAL4 binary enhancer trap Mi{ET1} GAL4 binary enhancer trap Mi{MIC} GFP gene trap, φC31 target

Drosophila Gene Search Project Constructs

P{GSV1} mobile activating element P{GSV2} mobile activating element P{GSV3} mobile activating element P{GSV6} mobile activating element P{GSV7} mobile activating element

Deletion Generators

P{XP} recombination PBac{RB} recombination PBac{WH} recombination P{RS5} recombination P{RS3} recombination PBac{5HPw[+]} recombination PBac{3HPy[+]} recombination P{wHy} hobo mobilization

Protein Trap Constructs

P{PTT-GA} GFP gene trap P{PTT-GB} GFP gene trap P{PTT-GC} GFP gene trap

Constructs Used for Other Collections

P{A92} lacZ enhancer trap P{lArB} lacZ enhancer trap P{lwB} lacZ enhancer trap P{hsneo} P{ry11} P{GawB} GAL4 binary enhancer trap PBac{SAstopDsRed} Tni\piggyBac mutator pP{MF3} VDRC-1 dsRNA construct collection vector pVALIUM10 TRiP-1 dsRNA construct collection vector

Genome Project Cloning Vectors

cDNA Cloning Vectors pOT2 pOTB7 pCDNA-SK[+] pCR2.1 pFLC-I pSport1-Tag21 BAC Vectors for Large Genomic Clones pBACe3.6

Basic Plasmid Cloning Vectors for P Transformation

Carnegie 20 Derivatives pP{Car20} ry+t7.2 pP{Car20.1} ry+t7.2 pP{DM30} ry+t7.2 CaSpeR Derivatives pP{CaSpeR} w+mC pP{CaSpeR-2} w+mC pP{CaSpeR-3} w+mC pP{CaSpeR-4} w+mC pP{CaSpeR-5} w+mC Others pP{hsneo} Tn\neoRHsp70Bb.PS pP{YES} y+mDint2 pP{W8} w+mW.hs pP{YC1.8} v+t1.8 pP{Car4} -none-

Plasmid Cloning Vectors with a Promoter

pP{CaSpeR-hs/act} Hsp70 pP{CaSpeR-hs} Hsp70 pP{CaSpeR-Hsp70/SV40} Hsp70 pP{Hsp70-CaSpeR} Hsp70 pP{W8H} Hsp70 pP{CaSpeR-act(B)} Act5C pP{CaSpeR-act(R)} Act5C pP{hsneo-act(B)} Act5C pP{hsneo-act(R)} Act5C pP{GMR} GMR (eye-specific) pP{COG} otu (oogenesis)

Plasmid Cloning Vectors with a Reporter Gene

pP{Pelican} LacZ pP{H-Pelican} LacZ pP{GreenPelican} GFP (vital reporter) pP{GreenH-Pelican} GFP (vital reporter) pP{Stinger} GFP (vital reporter) pP{H-Stinger} GFP (vital reporter) pP{RedH-Stinger} RFP (vital reporter) pP{RedH-Pelican} RFP (vital reporter) pP{YellowH-Pelican} YFP (vital reporter) pP{CaSpeR-lacZ} LacZ pP{CaSpeR-betagal} LacZ pP{CaSpeR-AUG-betagal} LacZ pP{DM79} LacZ pP{DM26} CAT pP{HZ50} LacZ pP{HZ50PL} LacZ pP{HZR} LacZ

Transposase Sources

pπ25.7wc P transposase pP{Δ2-3} P transposase pUChsΔ2-3 P transposase H{PΔ2-3} P transposase PBac{Δ2-3.Exel} P transposase pP{HBL1} hobo transposase pP{hsH\T-2} hobo transposase P{hsILMiT} Dhyd\Minos transposase P{Hsp70-PBac\T} Tni\piggyBac transposase P{Tub-PBac\T} Tni\piggyBac transposase

Cell Ablation Vectors and Constructs

pP{RMC4} pP{UAS-RAcs2} P{UAS-hid} P{UAS-hid.C} P{hs-hid} P{UAS-Hsap\KCNJ2.EGFP} P{UAS-NaChBac} P{UAS-Cbβ\DT-A.I} P{GMR-rpr.H} P{UAS-rpr.C} P{GMR-grim}

FLP/FRT Constructs

pP{hsFLP} P{70FLP} P{hsp70-flp} pP{neoFRT} P{FRT(whs)} pP{lacZ.FRT.B} P{lacZ.Act5C.B} pP{wg.FRT.S} pJ35 P{ey-FLP.N} P{ey3.5-FLP.Exel} P{ovo-FLP.R}

GAL4/UAS Cloning Vectors

pP{UAST} pP{UASp} pP{UAS-C5} pP{GawB} pP{WIZ} RNAi, UAS pP{PTGAL} P{GD264} pGaTN pGaTB

GAL4/UAS Target Constructs

P{UAS-lacZ.B} P{UAS-lacZ.Hsp70} P{UAS-lacZ.N} P{UAS-tau-lacZ.B} P{UAS-lacZ.NUC} P{UAS-GFP.S65T} P{UAS-RAcs2} Cell ablation P{AyGAL4} Mosaic analyses

Cell Culture Vectors

pMT-GAL4 pRmHa-1 pRmHa-3 pAct5CSRS pP-Ac pPacPL pBLCAT3 pP{C4cat} pSCAT0 pHGCO p0-CAT pcopneo

Targeted Replacement Vectors and Constructs

pP{W25} ends-out replacement pP{W30} ends-out replacement pP{W35} ends-out replacement P{70I-SceI} source of Scer\SCEI endonuclease P{hsFLP} source of Scer\FLP1 recombinase

φC31 Recombination-mediated genomic insertion
Cloning vectors for donor element

Pacman1-ApR BAC cloning vector Pacman1-CmR BAC cloning vector Pacman2-ApR BAC cloning vector Pacman2-CmR BAC cloning vector Pacman2-CmR-BW BAC cloning vector pUASTattB plasmid cloning vector pwP-Ex2UASTattB plasmid cloning vector; split-white system

Target elements

PBac{y+-attP-3B} target element PBac{y+-attP-9A} target element M{wEx3-6.attP} target element, split-white system M{loxP.attP} target element M{3xP3-RFP.attP} target element M{3xP3-RFP.attP'} target element P{CaryP} target element P{UASTP2} target element

Integrase sources

M{vas-int.B} integrase source (source within genome) M{vas-int.Dm} integrase source (source within genome) M{vas-int.NLS} integrase source (source within genome) M{nos-int.NLS} integrase source (source within genome) M{nos-int.B} integrase source (source within genome) pET11φC31poly(A) template for integrase mRNA production

Gateway recombinational cloning vectors (promoter; tag)

For a complete list of Gateway vectors please see the "Data from Reference" section of this page

Vector Promoter; Tag pP{TWG} UAS; EGFP pP{TGW} UAS; EGFP pAGW Act5C (cell culture); EGFP pAWG Act5C (cell culture); EGFP pAHW Act5C (cell culture); HA1 pP{TMW} UAS; Myc pP{TWM} UAS; Myc pP{TWV} UAS; YFP pP{TVW} UAS; YFP pP{THW} UAS; HA1 pP{TWH} UAS; HA1 pP{TW} UAS; [none] pP{PWG} UASp (female germline); EGFP pP{PGW} UASp (female germline); EGFP

Listing of all resources

Drosophila Network Resources

Comparative Analyses and Genome Databases

   Assembly/Alignment/Annotation of 12 related Drosophila species:
   »Assembly/Alignment/Annotation, LBNL, USA
   BDGP Single Nucleotide Polymorphism (SNP) Project:
   »Berkeley Drosophila Genome Project, University of California, Berkeley, USA
   Berkeley Drosophila Genome Project (BDGP):
   »BDGP, University of California, Berkeley, USA
   BDTNP, ChIP/chip in vivo DNA binding data:
   »Berkeley Drosophila Transcription Network Project, University of California, Berkeley, USA
   CluSTr protein sequence similarity analysis of Drosophila:
   »CluSTr proteome analysis, EBI, UK
   D. pseudoobscura genome project:
   »Human Genome Sequencing Center, Baylor College of Medicine, USA
   D. simulans genome project:
   »Genome Sequencing Center, Washington University, USA
   D. yakuba genome project:
   »Genome Sequencing Center, Washington University, USA
   D.melanogaster UCSC Genome Browser Gateway:
   »University of California, Santa Cruz, USA
   DDBJ, the DNA Data Bank of Japan:
   »DDBJ, National Institute of Genetics, Mishima, Japan
   DNase I Footprint Database:
   »University of Manchester Bioinformatics Resources, University of Manchester, UK
   Drosophila Heterochromatin Genome Project (DHGP):
   »DHGP, Lawrence Berkeley National Laboratory, USA
   Drosophila melanogaster Exon Database (DEDB):
   »DEDB, National University of Singapore
   Drosophila Polymorphism Database:
   »Drosophila Polymorphism Database, Univ. Autònoma de Barcelona, Spain
   Drosophila Population Genomic Project (DPGP):
   »DPGP, University of California, Davis, USA
   Drosophila simulans and yakuba sequencing projects:
   »DPGP, University of California, Davis, USA
   Drosophilidae Taxonomic Database:
   »Drosophilidae Taxonomic Database, Japan
   Drosophilidae Taxonomy - Collection of Papers:
   »Japan Drosophila Database (JDD) on Taxonomy, Japan
   DRSC, Drosophila RNAi Screening Center:
   »DRSC, Harvard Medical School, Boston, MA, USA
   EMBL Nucleotide Sequence Database:
   »EMBL Nucleotide Sequence Database, EBI, Cambridge, UK
   EPD, the Eukaryotic Promoter Database:
   »EPD, Epalinges s/Lausanne, Switzerland
   euGenes, Genomic Information for Eukaryotic Organisms, including Drosophila:
   » euGenes, Indiana University, USA
   EvoPrinter, Identification of Evolutionarily Resilient DNA Sequences
   »NINDS, NIH, Bethesda MD, USA
   FLIGHT, Fly database for the Integration of Genomic and High-Throughput data:
   »FLIGHT Database, Ludwig Institute for Cancer Research, London, UK
   FlyAtlas, the Drosophila adult gene expression atlas:
   »FlyAtlas, University of Glasgow, UK
   FlyMine, an integrated database for Drosophila & Anopheles genomics:
   »University of Cambridge, United Kingdom
   FlyPhenomics, phenotypic differences between species of the D. melanogaster subgroup:
   »FlyPhenomics, Paris, France
   FlySNP Project:
   »FlySNP, Austrian Academy of Sciences, Austria
   GenBank,the NIH genetic sequence database:
   »GenBank, NCBI, Bethesda, MD USA
   GenomeRNAi, Heidelberg, a database of RNA interference (RNAi) screens in cultured Drosophila cells:
   »GenomeRNAi, DKFZ, Heidelberg, Germany
   GEO, NCBI's Gene Expression Omnibus:
   »GEO, NCBI, Bethesda, MD USA
   GPCRDB, information system for G protein-coupled receptors (GPCRs):
   »GPCRDB, The GPCRDB (G Protein-Coupled Receptor Data Base) partnership
   Homophila, Human Disease to Drosophila Gene Database:
   » Homophila, San Diego, USA
   InParanoid, Eukaryotic Ortholog Groups:
   »InParanoid, Karolinska Institute, Sweden
   InterPro protein domain analysis of Drosophila:
   »InterPro proteome analysis, EBI, UK
   MANTiS a phylogenetic framework for multi-species genome comparisons:
   »MANTiS, Université Libre de Bruxelles, Belgium
   MEROPS, an information resource for peptidases:
   »MEROPS, The Sanger Institute, Cambridge, UK
   miRBase, microRNA data:
   »miRBase, The Sanger Institute, Cambridge, UK
   miRNA, Target Gene Prediction:
   »miRNA - Target Prediction, EMBL, Heidelberg, Germany
   MitoDrome, Drosophila melanogaster nuclear genes encoding for mitochondrial proteins:
   »MitoDrome, Bari, Italy
   Mitotic Spindle Assembly Database:
   »Genes Required for Mitotic Spindle Assembly in S2 cells, University of California, San Francisco, USA
   NHGRI Drosophila Genome Sequencing Proposals, Status of Sequencing:
   »National Human Genome Research Institute, Bethesda, MD, USA
   Panther protein classification system:
   »Panther Protein Classification System, Applied Biosystems & Celera, USA
   PDB, Protein Data Bank:
   »PDB, The Research Collaboratory for Structural Bioinformatics (RCSB) consortium
   PhospoPep - phosphorylation site data from D. melanogaster cell lines:
   »The Aebersold group at the ETH in collaboration with the FGCZ in Switzerland, and the ISB, USA.
   Proteome analysis of Drosophila:
   »Proteome analysis, EBI, UK
   PubMed, Biomedical literature citations and abstracts:
   »PubMed, NCBI, Bethesda, MD USA
   REDfly, Regulatory Element Database for Drosophila:
   »REDfly, University at Buffalo, New York, USA
   Rfam, RNA families database of alignments and CMs:
   »Rfam, the Sanger Institute, Cambridge, UK and Janelia Farm, Ashburn, VA, USA
   Sebida, Sex Bias Database:
   »Sebida, MPI Martinsried, Germany
   TaxoDros, the database on Taxonomy of Drosophilidae:
   »TaxoDros, University of Zurich, Switzerland
   The Drosophila Transcription Factor Database:
   »FlyTF.org, MRC Cambridge, UK
   The McGill Drosophila melanogaster Genome Project:
   »McGill Drosophila Genome Project, Canada
   TRANSFAC, A database of transcription factors and their binding sites:
   »TRANSFAC, Braunschweig, Germany
   TransTerm, a Translational Signal Database:
   »TransTerm, Dunedin, New Zealand
   UniProtKB, a comprehensive catalog of information on proteins:
   »UniProtKB, The UniProt Consortium
   Whole genome comparative analysis of D. melanogaster and D. pseudoobscura:
   »Whole genome analysis, LBNL, USA