FlyBase:Drosophila Material Resources

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Genomic Clones

BAC Genomic clones and Filters

  • The RPCI-98 Drosophila melanogaster BAC Library (17,540 clones in pBACe3.6; average clone size is 160-Kb), which was made from DNA of the same y1 ; cn1 bw1 sp1 stock as that used for the Berkeley Drosophila Genome Project P1 clones, is available from BACPAC Resources. This BACR library was made for the BDGP by K. Osoegawa, A. Mammoser, and P. de Jong.
  • Clones from two genomic BAC libraries (83 kb and 21 kb average clone size) engineered into the attB-P[acman]-CmR-BW vector, a special adaptation of P[acman], are also available from P(acman) Resources.
  • For information and resources related to the P[acman] recombineering technique see: P(acman) Resources

YAC Genomic clones

A complete set of YAC clones is maintained by Ian Duncan and clones may be requested from him.

Contact:

Ian W. Duncan,
Department of Biology,
Box 1137,
Washington University,
St. Louis, MO 63130-4899.
Telephone (1)-314-935-6719;
fax(1)-314-935-4432;
e-mail duncan@biology.wustl.edu.

Sibling Species Genomic Libraries

Fosmid Libraries from BACPAC Resources

Genomic fosmid libraries were constructed for the BDGP by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila erecta, D. pseudoobscura, D. virilis, and D. willistoni. The fosmid libraries are available from BACPAC Resources

cDNA (EST Clones and Libraries)

  • Addgene
    Addgene helps labs archive and share their plasmids that have appeared in publications.
    addgene
  • Berkeley Drosophila Genome Project
    Information on cDNA availability can also be found here BDGP Resources.
  • Brian Oliver Laboratory
    Testis cDNA clones and filters constructed by Justen Andrews in Brian Oliver's laboratory at the NIH are available from GenomeCUBE at Source BioScience in the UK.
    Ordering information can be found at Drosophila cDNA Library NIH.
  • cDNA library aliquots
    Aliquots from a number of the BDGP cDNA libraries are available from the labs on this list.
  • Drosophila Gene Collection (DGC)
    Contact BACPAC Resources, GenomeCUBE, or the DGRC
    for clones in DGC Releases 1,2 or 3.
  • Drosophila Genomics Resource Center (DGRC)
    Collects and distributes DNA clones, including vectors, cDNAs, and ESTs. More information is available at DGRC.
  • EST Project cDNA Clones
    Contact the DGRC for cDNA clones from the BDGP EST collection.

Arrays and Primers

  • Affymetrix
    The Affymetrix GeneChip Drosophila Genome 2.0 Array for gene expression analysis based on Release 3.1 annotations can be obtained at Genome 2.0 Array.
    The Affymetrix GeneChip Drosophila Tiling 2.0R Array based on FlyBase Release 5 with comprehensive genome coverage at 38bp resolution can be obtained at Tiling 2.0R Array.
  • Drosophila Genomics Resource Center (DGRC)
    Produces and distributes DNA microarray slides. More information is available at DGRC.
  • FlyChip
    FlyChip is a publicly funded project to provide a non-profit microarray resource for the UK Drosophila research community. More information is available at FlyChip.
  • International Drosophila Array Consortium (INDAC)
    An international consortium to collaborate in the development and aquisition of microarray resources for the benefit of the entire research community. More information is available at FlyChip_long_oligonucleotide_003 (FL003) - INDAC.
  • UK Drosophila Affymetrix Microarray Service
    A publicly funded microarray service for the UK Drosophila research community. More information is available at FlyChip Affymetrix
  • FlyPrimerBank
    An online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. More information is available at DRSC FlyPrimer Bank

Stocks

  • Bloomington Stock Center
    Information about stocks at the Bloomington Drosophila Stock Center (BDSC) at Indiana University, USA can be obtained at BDSC
  • CRISPR Stocks - Bloomington Stock Center
    CRISPR system stocks for site-specific mutagenesis can be obtained at BDSC.
  • CRISPR Stocks - Description of CRISPR fly design cas9 stocks
    CRISPR fly design.
  • DrosDel Project
    Information about isogenic deficiency lines generated by the DrosDel Project at the University of Cambridge, UK can be found at DrosDel.
  • Drosophila Gene Search Project
    Information about ordering P{GS} lines can be found at Kyoto Stock Center DGGR.
  • DSSC, Drosophila Species Stock Center
    Approximately 270 different Drosophila species at the University of San Diego Drosophila Stock Center can be obtained at DSSC.
  • EHIME-Fly, Ehime Drosophila Species Stock Center of Japan
    A collection of wild-type strains of D. melanogaster and strains of over 130 species can be found at EHIME-Fly.
  • Exelixis Collection at Harvard Medical School
    Information about ordering the Exelixis stocks at Harvard Medical School can be found at Exelixis Collection.
  • FlyORF
    A collection of well-characterized transgenic D. melanogaster UAS-ORF lines produced and distributed by the Zurich ORFeome Project at FlyORF.
  • FlyTrap Stock Collection
    Information about GFP protein trap lines from the FlyTrap project at FlyTrap.
  • FlyView Stock Collection
    Enhancer trap lines from the FlyView project can be obtained at University of Muenster, Germany at FlyView.
  • Gene Disruption Project (GDP)
    Information about insertion lines produced by the Gene Disruption Project (GDP), Baylor College of Medicine, Texas, USA can be found at GDP.
  • Kyorin-Fly
    A collection of mutant and wildtype stocks of Drosophila species, especially the Drosophila ananassae species subgroup, D. auraria complex, and D. hydei. Kyorin-Fly.
  • Kyoto Stock Center, DGGR
    Information about stocks at the Department of Drosophila Genomics and Genetics Resources (DGGR) at the Kyoto Institute of Technology, Japan can be found at Kyoto Stock Center, DGGR.
  • MiMIC RMCE Lines
    Information about a collection of protein trap lines containing the EGFP-FlAsH-Strep-TEV-3xFlag tag using the RMCE technique at />GDP
  • NIG-FLY: Fly Stocks of the National Institute of Genetics
    Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at NIG-Fly.
  • Tsinghua Fly Center
    Information about RNAi lines at the Tsinghua Fly Center (THFC) at the Tsinghua University, Beijing, China can be found at THFC.
  • Vienna Drosophila RNAi Center
    The VDRC at IMP/IMBA in Vienna provides two genome-wide transgenic Drosophila RNAi libraries at VDRC.

CRISPR Vectors

  • CRISPR/Cas Vectors from Simon Bullock Lab
    A set tools for efficient germline and somatic genome engineering in Drosophila melanogaster, available at the Addgene plasmid repository, Cambridge, MA, USA
  • CRISPR Plasmids - CRISPR fly design cas9 plasmids description
    CRISPR fly design, Simon Bullock, MRC, Cambridge, UK

Expression Vectors

  • Drosophila Gateway Vectors
    A set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies, available at the DGRC
  • ExpreS2ion Biotechnologies
    ExpreS2ion Biotechnologies has developed an "improved drosophila S2 insect cell expression system" and are offering services such as protein expression and purification. The ExpreS2 Platform

Cell Lines and Hybridomas

  • Drosophila Genomics Resource Center (DGRC)
    Collects and distributes cell lines. More information is available at DGRC
  • Developmental Studies Hybridoma Bank
    The Developmental Studies Hybridoma Bank at the University of Iowa offers hybridomas and antibodies at DSHB.
  • ATCC
    The American Type Culture Collection (ATCC) provides Schneider's L-2 and L-2/M delta 2-3 cell lines and hybridomas at ATCC.

Antibodies

Transgenic, Targeted Mutation, and Other Services

  • Aktogen behaviour genetic services
    Aktogen at Cambridge University offers a wide range of services to the fruit fly community such as behaviour tests, behaviour and other equipment, custom made genetic constructs and transgenic services. Aktogen
  • BestGene Transgenic Service
    The BestGene Drosophila Embryo Injection service provides partial to full service ranging from DNA preparation, embryo microinjection to balancing crosses. More information is available at BestGene.
  • C-CAMP Centre for Cellular and Molecular Platforms
    A Bangalore-based fly facility housed in NCBS offering Drosophila services ranging from transgenesis to screening. More information is available at C_CAMP.
  • Drosophila TILLING Project
    The goal of Fly-TILL is to rapidly deliver to the Drosophila community EMS-induced mutations in requested genes via high-throughput reverse genetic screens. More information is available at Fly-TILL.
  • Fly Facility
    Fly Facility located at the Universite d'Auvergne Clermont-Ferrand I, France, provides a transgenesis and dsRNA microinjection service. More information is available at Fly Facility.
  • Genetic Services, Inc.
    Genetic Services provides the Drosophila community with services including transgenic production, injection work, genetic screening, stock creation, construct production and consulting. More information is available at Genetic Services, Inc.
  • GenetiCorps Custom Injection Services
    GenetiCorps offers high quality fly injection services to the Drosophila research community. For more information, please visit GenetiCorps.
  • Genetivision, Inc.
    Genetivision offers a transgenic fly service for both delta 2-3 transposase-mediated P-element transgenesis and Phi-C31 mediated site-specific integration. More information is available at Genetivision.
  • The Genome Engineering Production Group
    The GEPG at Harvard Medical School currently builds custom CRISPR sgRNA constructs for knock-out, knock-in or other projects. GEPB
  • GenScript
    A US based company offering gene construction, mutagenesis and subcloning services, as well as a large array of other products and services. GenScript
  • Rainbow Transgenic Flies
    Rainbow Transgenic Flies provides professional microinjection services to the fly community. More information is available at Rainbow Transgenic Flies.
  • Union Biometrica
    COPAS Select flow cytometry instruments are able to analyze and dispense Drosophila embryos on the basis of size and fluorescent signals. More information is available at Union Biometrica.

RNA

Clontech
Clontech of BD Biosciences offers adult, embryo, and larva Poly A RNA at http://www.clontech.com/products/detail.asp?product_id=167106&tabno=2

RNAi

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